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The map label for this gene is ydaB [H]

Identifier: 153932963

GI number: 153932963

Start: 2640902

End: 2642440

Strand: Reverse

Name: ydaB [H]

Synonym: CLB_2525

Alternate gene names: 153932963

Gene position: 2642440-2640902 (Counterclockwise)

Preceding gene: 153933885

Following gene: 153931739

Centisome position: 68.4

GC content: 25.73

Gene sequence:

>1539_bases
ATGAAAGAAAAGGTTACTTTTGGAGATATATATACTGTAATTTTTCAAGAGGAAGATAGTAATAAAGAATGTATAATAGT
CTACAATGATGAAAAAAGGATTGTGTACAATAAAAAACAATATCAAATGTTGATTAATAAAATGAGTAGATTTTTAAAAC
TTCTTTATCCAAATATAAAAGTCGGAGATTGGATTGGTTTGAAATATCATAATAACTATCTCTATTATGCAGTAGTATTT
GGGGCTATGAAATTAGGATGGAATATATTATTACTTGACAATAAAAGTGAAAGTAATAATAGGTATAGATATGAGATAGA
TGTGGAGTTAATTGCTATTTTAGATAATAATATATTAGATAATTTTAATAATGAAGCTATTGATATTCAATGTTTAAATA
GTAAGTTTGAGAAAGATATTTGGTCCAGCAAAGTAGCATTTTTATCTTCTGGAACAACAGGAAAGCCACAAGTTATTATA
TATCATGCTTATGCAATTTTAAATCAAATAAAAAATGTTAAATATGAATTTTTAAGAAATGAGACGTTAGCAGATATTGT
GTATAAAACTGATAATTCGTACAGAAAAATAGTAACTTTTTTACCTATGAATCATATTTTAGGTTTTTTATTACCTTTAA
TTTTCATCACTTTAGACTTAAAGGTTGTTTTTCTATCAGATCAAACACCATATGGTATTATTAAAGGAATAAGAAATGAG
AATGCTTTAGGAAGTTTTGGTGTGCCTATGGTTTGGAAATTAATAAAAAATATATTAAAGAGAAGGTATTCCGAAAATCG
TGAAGGTGTAGATAAATTATTAGGGGAAAATTTTAAAATTATTTTAAGTGGTGGCAGTAAAACAGATGCTTCCATTAGAA
ATAGTTATATTAGAGCAGGAATTGAATTTTTCGTTGGTTATGGATTAACAGAAACTGGTTTTTTATCTATAGGCAAAAGT
GATCTAAATGACTGTGAGTCTGAAGGAAAAGTTTATGATAATTATGAAATTAAAGTTTTAACTTCAGATGGAAGTATAAG
AAATGAAGGTACTGGAGAAATTATCGTAAAATCAGATTCTATATACGCTGCTTCCATAAAAGATAAAAAAGAAATTTGCC
CTAAATTAATTGAAGGCATGTATTATAGAACAGGGGATATCTTTGAGCTAAAAAATAGAAAACTTTATTTTAAAGGTAGG
ATGAAAAATATTATTGTAGATGAATCTGGAGAAAATATATATGCTGAAGAATTAGAAGAATTTTTTAAAGACATGAAGTC
TAAAGGGATTGTATATTTTATATCAGAATATAAAAATAGGGCTACTATGTTCATTGAGAGGAATACACATAAACGCATTT
CAAAGAAATATATTATTGAGCTAATAAGAGAAAAAAATAAGAATCTTCCTATTTCAAAGAAAATATTAAAAGTATTATTT
TTAAATATAAATATTGAATTTACTCAAAAGGGAGATCCTATCCGTATTCTGAAAGAATACAGGGATATTGGTGAAGTATT
TAATATAAAAGGAGTATGA

Upstream 100 bases:

>100_bases
GGAAGATTAAAGACTGGAAGTAAAACTTTGATGTCTGGTGTTGGAGTTGGAATTAATATCGCGACTATCGCAGTAATACT
TTAGTTATGTAGGTGAGTAT

Downstream 100 bases:

>100_bases
TAATTATGATAGATAATAATACAAAAATTGGGCTAAAAAAACAAATTAAAAAATATCTATCAGAGCTAATGGATGTTAAT
ACTGAAGATATTATGGATGA

Product: putative acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKEKVTFGDIYTVIFQEEDSNKECIIVYNDEKRIVYNKKQYQMLINKMSRFLKLLYPNIKVGDWIGLKYHNNYLYYAVVF
GAMKLGWNILLLDNKSESNNRYRYEIDVELIAILDNNILDNFNNEAIDIQCLNSKFEKDIWSSKVAFLSSGTTGKPQVII
YHAYAILNQIKNVKYEFLRNETLADIVYKTDNSYRKIVTFLPMNHILGFLLPLIFITLDLKVVFLSDQTPYGIIKGIRNE
NALGSFGVPMVWKLIKNILKRRYSENREGVDKLLGENFKIILSGGSKTDASIRNSYIRAGIEFFVGYGLTETGFLSIGKS
DLNDCESEGKVYDNYEIKVLTSDGSIRNEGTGEIIVKSDSIYAASIKDKKEICPKLIEGMYYRTGDIFELKNRKLYFKGR
MKNIIVDESGENIYAEELEEFFKDMKSKGIVYFISEYKNRATMFIERNTHKRISKKYIIELIREKNKNLPISKKILKVLF
LNINIEFTQKGDPIRILKEYRDIGEVFNIKGV

Sequences:

>Translated_512_residues
MKEKVTFGDIYTVIFQEEDSNKECIIVYNDEKRIVYNKKQYQMLINKMSRFLKLLYPNIKVGDWIGLKYHNNYLYYAVVF
GAMKLGWNILLLDNKSESNNRYRYEIDVELIAILDNNILDNFNNEAIDIQCLNSKFEKDIWSSKVAFLSSGTTGKPQVII
YHAYAILNQIKNVKYEFLRNETLADIVYKTDNSYRKIVTFLPMNHILGFLLPLIFITLDLKVVFLSDQTPYGIIKGIRNE
NALGSFGVPMVWKLIKNILKRRYSENREGVDKLLGENFKIILSGGSKTDASIRNSYIRAGIEFFVGYGLTETGFLSIGKS
DLNDCESEGKVYDNYEIKVLTSDGSIRNEGTGEIIVKSDSIYAASIKDKKEICPKLIEGMYYRTGDIFELKNRKLYFKGR
MKNIIVDESGENIYAEELEEFFKDMKSKGIVYFISEYKNRATMFIERNTHKRISKKYIIELIREKNKNLPISKKILKVLF
LNINIEFTQKGDPIRILKEYRDIGEVFNIKGV
>Mature_512_residues
MKEKVTFGDIYTVIFQEEDSNKECIIVYNDEKRIVYNKKQYQMLINKMSRFLKLLYPNIKVGDWIGLKYHNNYLYYAVVF
GAMKLGWNILLLDNKSESNNRYRYEIDVELIAILDNNILDNFNNEAIDIQCLNSKFEKDIWSSKVAFLSSGTTGKPQVII
YHAYAILNQIKNVKYEFLRNETLADIVYKTDNSYRKIVTFLPMNHILGFLLPLIFITLDLKVVFLSDQTPYGIIKGIRNE
NALGSFGVPMVWKLIKNILKRRYSENREGVDKLLGENFKIILSGGSKTDASIRNSYIRAGIEFFVGYGLTETGFLSIGKS
DLNDCESEGKVYDNYEIKVLTSDGSIRNEGTGEIIVKSDSIYAASIKDKKEICPKLIEGMYYRTGDIFELKNRKLYFKGR
MKNIIVDESGENIYAEELEEFFKDMKSKGIVYFISEYKNRATMFIERNTHKRISKKYIIELIREKNKNLPISKKILKVLF
LNINIEFTQKGDPIRILKEYRDIGEVFNIKGV

Specific function: Unknown

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 59520; Mature: 59520

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKVTFGDIYTVIFQEEDSNKECIIVYNDEKRIVYNKKQYQMLINKMSRFLKLLYPNIK
CCCCEEECCEEEEEEEECCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHCCCCE
VGDWIGLKYHNNYLYYAVVFGAMKLGWNILLLDNKSESNNRYRYEIDVELIAILDNNILD
ECCEEEEEEECCEEEEHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEEEEEECCHHHC
NFNNEAIDIQCLNSKFEKDIWSSKVAFLSSGTTGKPQVIIYHAYAILNQIKNVKYEFLRN
CCCCCEEEEEEECCHHHHHHHHHHEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
ETLADIVYKTDNSYRKIVTFLPMNHILGFLLPLIFITLDLKVVFLSDQTPYGIIKGIRNE
CCCEEEEEECCCCHHHHEEECCHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHCCCCC
NALGSFGVPMVWKLIKNILKRRYSENREGVDKLLGENFKIILSGGSKTDASIRNSYIRAG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
IEFFVGYGLTETGFLSIGKSDLNDCESEGKVYDNYEIKVLTSDGSIRNEGTGEIIVKSDS
HHHHEECCCCCCCEEEECCCHHHHHHHCCCEEECEEEEEEECCCCCCCCCCCEEEEECCC
IYAASIKDKKEICPKLIEGMYYRTGDIFELKNRKLYFKGRMKNIIVDESGENIYAEELEE
EEEECCCCHHHHHHHHHHCCEECCCCEEEECCCEEEEECCCCEEEEECCCCEEHHHHHHH
FFKDMKSKGIVYFISEYKNRATMFIERNTHKRISKKYIIELIREKNKNLPISKKILKVLF
HHHHHHHCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEE
LNINIEFTQKGDPIRILKEYRDIGEVFNIKGV
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKEKVTFGDIYTVIFQEEDSNKECIIVYNDEKRIVYNKKQYQMLINKMSRFLKLLYPNIK
CCCCEEECCEEEEEEEECCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHCCCCE
VGDWIGLKYHNNYLYYAVVFGAMKLGWNILLLDNKSESNNRYRYEIDVELIAILDNNILD
ECCEEEEEEECCEEEEHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEEEEEECCHHHC
NFNNEAIDIQCLNSKFEKDIWSSKVAFLSSGTTGKPQVIIYHAYAILNQIKNVKYEFLRN
CCCCCEEEEEEECCHHHHHHHHHHEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
ETLADIVYKTDNSYRKIVTFLPMNHILGFLLPLIFITLDLKVVFLSDQTPYGIIKGIRNE
CCCEEEEEECCCCHHHHEEECCHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHCCCCC
NALGSFGVPMVWKLIKNILKRRYSENREGVDKLLGENFKIILSGGSKTDASIRNSYIRAG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
IEFFVGYGLTETGFLSIGKSDLNDCESEGKVYDNYEIKVLTSDGSIRNEGTGEIIVKSDS
HHHHEECCCCCCCEEEECCCHHHHHHHCCCEEECEEEEEEECCCCCCCCCCCEEEEECCC
IYAASIKDKKEICPKLIEGMYYRTGDIFELKNRKLYFKGRMKNIIVDESGENIYAEELEE
EEEECCCCHHHHHHHHHHCCEECCCCEEEECCCEEEEECCCCEEEEECCCCEEHHHHHHH
FFKDMKSKGIVYFISEYKNRATMFIERNTHKRISKKYIIELIREKNKNLPISKKILKVLF
HHHHHHHCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEE
LNINIEFTQKGDPIRILKEYRDIGEVFNIKGV
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]