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The map label for this gene is sigK [H]

Identifier: 153932837

GI number: 153932837

Start: 2593141

End: 2593575

Strand: Reverse

Name: sigK [H]

Synonym: CLB_2480

Alternate gene names: 153932837

Gene position: 2593575-2593141 (Counterclockwise)

Preceding gene: 153932020

Following gene: 153931679

Centisome position: 67.13

GC content: 31.03

Gene sequence:

>435_bases
GTGTTCTTTTTAAGTTACTTATGGGATGTACTAGGAAGTGCATTATTTTTAACAGGCTATGTTACAGGAAATACTTCTTT
CCCCAAGCCTCTAAGTGATGAAGAAGAAAAATACTATTTGGATAGGTTAAAAAAGGGTGATGCTTTAGCAAAAGATATCT
TAGTGGAAAGAAATTTAAGATTAGTGGCTCACATAGTAAAAAAATATTCTTATCCGGGAAAAGATATTGATGATTTAATA
TCTATAGGAACTGTAGGTCTTATAAAAGCTATAGACTCTTTTGATAATTCAAAGGGTACTAGACTTGCCACTTATGCAGC
AAGATGTATAGAAAATGAAATATTAATGCTCATAAGAAATAATAAAAAAACTAAAGGTGAGGTATATCTCCAAGATCCTA
TAGGAGTAGACAAAGAAGGAAATGAATACCCTTAA

Upstream 100 bases:

>100_bases
ATTGTCTTTTAATGCCTAATGGAAAATATGATTTTAAATATGCAACTAGTACAAGCTTATTGAATATTATATTAATAAGC
ACTATTAGGAGGAGCAGCTT

Downstream 100 bases:

>100_bases
ACATGTTATATGTTTAAGGGTTTTAATTTAGGAAATACCTTGATTTTGAAGTCATTCCCCTTTTTATTCTTAGTATATTC
TATTTTAAAAATCAAGGTCT

Product: RNA polymerase sigma-K factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLRLVAHIVKKYSYPGKDIDDLI
SIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRNNKKTKGEVYLQDPIGVDKEGNEYP

Sequences:

>Translated_144_residues
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLRLVAHIVKKYSYPGKDIDDLI
SIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRNNKKTKGEVYLQDPIGVDKEGNEYP
>Mature_144_residues
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLRLVAHIVKKYSYPGKDIDDLI
SIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRNNKKTKGEVYLQDPIGVDKEGNEYP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=121, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=3e-15,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 16274; Mature: 16274

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLR
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHH
LVAHIVKKYSYPGKDIDDLISIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEEEC
NKKTKGEVYLQDPIGVDKEGNEYP
CCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLR
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHH
LVAHIVKKYSYPGKDIDDLISIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEEEC
NKKTKGEVYLQDPIGVDKEGNEYP
CCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]