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The map label for this gene is prdA [H]

Identifier: 153931385

GI number: 153931385

Start: 2486591

End: 2488702

Strand: Reverse

Name: prdA [H]

Synonym: CLB_2349

Alternate gene names: 153931385

Gene position: 2488702-2486591 (Counterclockwise)

Preceding gene: 153933696

Following gene: 153931867

Centisome position: 64.42

GC content: 33.62

Gene sequence:

>2112_bases
ATGTCAATGACAGCTGAACATGCAGAAGAATTAAAAAATGAACATGCTGTAGTTTGTTGTAGAACAGAAGAAGGAACAAT
ATTATCAGCAGATAATTTAGAAGATCCAGAAATATTTCCGGATATGGTAGATTCAGGACTATTAACTATCCCAGATAATT
GTTTGAAAGTTGGAGAAGTAATAGGTGCTAAATTGTTGAAAACTGTAGATTCATTAACTCCACTAACTTCAGATATAGTA
GAGGGTGCTAAAAAAATAGGCGAAGAAGATAAAGAAGATTCTACTACTGAAGAAGCATTATTGACTGAGGGAAATGAAAA
AGCAGTTTTAAAATATAACCTAAAAGCAGGAGATATTATAAAAGCTAGTGACTTAGAAAACCCAATGCATTTCCCTAAAC
TTGTAGATTCTCTTTTAATTTCATTAGATGAAAGAGTACTTACAAGAGAAGAAGTTGTAGGGGCTACATTAAATGTAGAT
ACACCAGCTTTAACTCCTGTAACACCAGATATATTAGAAGGATTTGAGGAGGAAGTAAACATGAGTGTTGATTCAAATGC
AACAGTAAGTGGTGGAACTTTAAGAATCAAAATTGCTGAAGGTAGAGGAATTGATATAGAAGTTCCTTTAAATGGCAATG
TAGGTGCTGGGAAATCAGCAAAGGTTCCAACAGTAAAGGCTGAAAAAGGTGAAGTAGTAGCAGCTCCAGCTGTAGAAGCC
AACCAAGAAGTTAAATTAGAAGAAAAAGTTATAAGAAGTCTTACTAGAAAACACTATAAAATAGACAAAATAGAATTTGG
CCCTGAAACAAAAATAGAAGGAACAACTCTTTATATAAGAGAAGGAGTTTCTGAAGATGCTAAAAAAGTTAGTCCACTTG
TAAAAAAAGTAGAAATAGATATAATAACTCCAGATAAATACAACACTTATAGTGAAACTATAATGGATGTACAACCAATT
GCTACTAAAGAAGGGGATAACAAATTAGGAACAGGTGCAACTAGAGTATTAGACGGTGTTATTGTAATGGTAACAGGAAC
AGATGAAAATGGTGTTCAAATAGGTGAATTTGGTTCTTCAGAAGGTATATTAGAAGAAAATATTATGTTTAATAGACCAG
GATCACCAGATAAAGGTGAAATATTCATCAAAACACAAGTAACTATAAAAGAAAAAACTAATATGGAAAGACCAGGTCCT
ATGGCTGCTCATAAAGCAACTGATTTCATAACTCAAGAAATAAGGGAAGCTATGAAAGCTTTAGAGGATGAGTCCTTAGT
AGTTAACACTGAAACTTTTGAACAAGTAAGAAGACCAGGAAAGAAAAAAGTTGTAGTTGTTAAAGAAATAATGGGTCAAG
GTGCTATGCACGACAATTTAATATTACCAGTAGAACCAGTAGGTTATTTAGGCGGTAAGCCAAATGTTGATTTAGGAAAT
GTTCCAGTAATGCTTTCACCACTTGAAGTATTAGATGGTGGTATACATGCATTAACTTGTATTGGACCTGCTTCTAAAGA
ATGTTCAAGACATTATTGGAGAGAACCTCTTGTAATGGAATGCATGAAGGATGAAGAATTAGATTTATGCGGAGTTATAT
TTGTAGGAAGTCCACAAATTAACAGTGAAAAATTCTATGTATCAGAAAGAATGGGTATGATGGTAGAAGCTTTAGATGTT
GATGGTGCTTTTGTTACAACTGAAGGCTTTGGAAATAACCACATTGACTTTGCAAGTCACGTAGAACAAATAGGTATGAG
AGGAATACCATGTGTAGCATTCTCATTCTGTGCAGTCCAAGGCGCTCTAGTTGTTGGTAATAAATACATGAAATATATGA
TAGATAATAACAAATCAGAAGGCGGAATAGAAAACGAAGTGCTAGAATGCAACACATTATGCCCAGAAGATGCTATAAGA
GGCGTTGAAATGCTTAAAGCTGCTATGACTGGAGAAGATGTTAAAAAACCAGAAAGAGCTTGGAATGCAAATGTTAAGGA
AAATAACTTAGAATTAATAGAAAAAGCTGAAAATGTGAAAATAGAAAGAGTCTTAAATGAAACTTCAATACCAATGAGTG
AAAAGAGAAAAGAAAAATACAGCACAAAGTAG

Upstream 100 bases:

>100_bases
ATATCATCATACTGACCATATATTTAGAACAATTGATTATACATTAACAAGAAGACTTAAAGAGGAAGAAAAATAATAAA
AAGTTAGGAGGTAATTTGAG

Downstream 100 bases:

>100_bases
GGATATAAATGGATTTAAAATACGGTGATAAGATCATAGAAATGGAAGGCGTTATCGTAGAAGTATCCGATGGTGCAGTA
GCTATAGATTTTAAAGGAAG

Product: D-proline reductase, PrdA proprotein

Products: NA

Alternate protein names: D-proline reductase 45 kDa subunit; D-proline reductase 23 kDa subunit [H]

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MSMTAEHAEELKNEHAVVCCRTEEGTILSADNLEDPEIFPDMVDSGLLTIPDNCLKVGEVIGAKLLKTVDSLTPLTSDIV
EGAKKIGEEDKEDSTTEEALLTEGNEKAVLKYNLKAGDIIKASDLENPMHFPKLVDSLLISLDERVLTREEVVGATLNVD
TPALTPVTPDILEGFEEEVNMSVDSNATVSGGTLRIKIAEGRGIDIEVPLNGNVGAGKSAKVPTVKAEKGEVVAAPAVEA
NQEVKLEEKVIRSLTRKHYKIDKIEFGPETKIEGTTLYIREGVSEDAKKVSPLVKKVEIDIITPDKYNTYSETIMDVQPI
ATKEGDNKLGTGATRVLDGVIVMVTGTDENGVQIGEFGSSEGILEENIMFNRPGSPDKGEIFIKTQVTIKEKTNMERPGP
MAAHKATDFITQEIREAMKALEDESLVVNTETFEQVRRPGKKKVVVVKEIMGQGAMHDNLILPVEPVGYLGGKPNVDLGN
VPVMLSPLEVLDGGIHALTCIGPASKECSRHYWREPLVMECMKDEELDLCGVIFVGSPQINSEKFYVSERMGMMVEALDV
DGAFVTTEGFGNNHIDFASHVEQIGMRGIPCVAFSFCAVQGALVVGNKYMKYMIDNNKSEGGIENEVLECNTLCPEDAIR
GVEMLKAAMTGEDVKKPERAWNANVKENNLELIEKAENVKIERVLNETSIPMSEKRKEKYSTK

Sequences:

>Translated_703_residues
MSMTAEHAEELKNEHAVVCCRTEEGTILSADNLEDPEIFPDMVDSGLLTIPDNCLKVGEVIGAKLLKTVDSLTPLTSDIV
EGAKKIGEEDKEDSTTEEALLTEGNEKAVLKYNLKAGDIIKASDLENPMHFPKLVDSLLISLDERVLTREEVVGATLNVD
TPALTPVTPDILEGFEEEVNMSVDSNATVSGGTLRIKIAEGRGIDIEVPLNGNVGAGKSAKVPTVKAEKGEVVAAPAVEA
NQEVKLEEKVIRSLTRKHYKIDKIEFGPETKIEGTTLYIREGVSEDAKKVSPLVKKVEIDIITPDKYNTYSETIMDVQPI
ATKEGDNKLGTGATRVLDGVIVMVTGTDENGVQIGEFGSSEGILEENIMFNRPGSPDKGEIFIKTQVTIKEKTNMERPGP
MAAHKATDFITQEIREAMKALEDESLVVNTETFEQVRRPGKKKVVVVKEIMGQGAMHDNLILPVEPVGYLGGKPNVDLGN
VPVMLSPLEVLDGGIHALTCIGPASKECSRHYWREPLVMECMKDEELDLCGVIFVGSPQINSEKFYVSERMGMMVEALDV
DGAFVTTEGFGNNHIDFASHVEQIGMRGIPCVAFSFCAVQGALVVGNKYMKYMIDNNKSEGGIENEVLECNTLCPEDAIR
GVEMLKAAMTGEDVKKPERAWNANVKENNLELIEKAENVKIERVLNETSIPMSEKRKEKYSTK
>Mature_702_residues
SMTAEHAEELKNEHAVVCCRTEEGTILSADNLEDPEIFPDMVDSGLLTIPDNCLKVGEVIGAKLLKTVDSLTPLTSDIVE
GAKKIGEEDKEDSTTEEALLTEGNEKAVLKYNLKAGDIIKASDLENPMHFPKLVDSLLISLDERVLTREEVVGATLNVDT
PALTPVTPDILEGFEEEVNMSVDSNATVSGGTLRIKIAEGRGIDIEVPLNGNVGAGKSAKVPTVKAEKGEVVAAPAVEAN
QEVKLEEKVIRSLTRKHYKIDKIEFGPETKIEGTTLYIREGVSEDAKKVSPLVKKVEIDIITPDKYNTYSETIMDVQPIA
TKEGDNKLGTGATRVLDGVIVMVTGTDENGVQIGEFGSSEGILEENIMFNRPGSPDKGEIFIKTQVTIKEKTNMERPGPM
AAHKATDFITQEIREAMKALEDESLVVNTETFEQVRRPGKKKVVVVKEIMGQGAMHDNLILPVEPVGYLGGKPNVDLGNV
PVMLSPLEVLDGGIHALTCIGPASKECSRHYWREPLVMECMKDEELDLCGVIFVGSPQINSEKFYVSERMGMMVEALDVD
GAFVTTEGFGNNHIDFASHVEQIGMRGIPCVAFSFCAVQGALVVGNKYMKYMIDNNKSEGGIENEVLECNTLCPEDAIRG
VEMLKAAMTGEDVKKPERAWNANVKENNLELIEKAENVKIERVLNETSIPMSEKRKEKYSTK

Specific function: D-proline reductase catalyzes the reductive cleavage of a C-N bond in D-proline resulting in the formation of 5- aminovalerate. The alpha subunit has been shown to bind D-proline, presumably via the pyruvoyl group [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015417 [H]

Pfam domain/function: PF09338 Gly_reductase [H]

EC number: =1.21.4.1 [H]

Molecular weight: Translated: 76869; Mature: 76738

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTAEHAEELKNEHAVVCCRTEEGTILSADNLEDPEIFPDMVDSGLLTIPDNCLKVGEV
CCCCHHHHHHHCCCCEEEEEECCCCCEEECCCCCCCCCCHHHHCCCCEECCHHHHHHHHH
IGAKLLKTVDSLTPLTSDIVEGAKKIGEEDKEDSTTEEALLTEGNEKAVLKYNLKAGDII
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCEE
KASDLENPMHFPKLVDSLLISLDERVLTREEVVGATLNVDTPALTPVTPDILEGFEEEVN
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHCHHHHCC
MSVDSNATVSGGTLRIKIAEGRGIDIEVPLNGNVGAGKSAKVPTVKAEKGEVVAAPAVEA
CCCCCCCEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCCEEEECCCCC
NQEVKLEEKVIRSLTRKHYKIDKIEFGPETKIEGTTLYIREGVSEDAKKVSPLVKKVEID
CCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHEEEEE
IITPDKYNTYSETIMDVQPIATKEGDNKLGTGATRVLDGVIVMVTGTDENGVQIGEFGSS
EECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCEEECCCCCC
EGILEENIMFNRPGSPDKGEIFIKTQVTIKEKTNMERPGPMAAHKATDFITQEIREAMKA
CCCCCCCEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LEDESLVVNTETFEQVRRPGKKKVVVVKEIMGQGAMHDNLILPVEPVGYLGGKPNVDLGN
CCCCCEEEEHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCC
VPVMLSPLEVLDGGIHALTCIGPASKECSRHYWREPLVMECMKDEELDLCGVIFVGSPQI
CCEEECCHHHHCCCCEEEEEECCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCCC
NSEKFYVSERMGMMVEALDVDGAFVTTEGFGNNHIDFASHVEQIGMRGIPCVAFSFCAVQ
CCCEEEHHHHHCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
GALVVGNKYMKYMIDNNKSEGGIENEVLECNTLCPEDAIRGVEMLKAAMTGEDVKKPERA
CEEEECCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCHHH
WNANVKENNLELIEKAENVKIERVLNETSIPMSEKRKEKYSTK
HCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SMTAEHAEELKNEHAVVCCRTEEGTILSADNLEDPEIFPDMVDSGLLTIPDNCLKVGEV
CCCHHHHHHHCCCCEEEEEECCCCCEEECCCCCCCCCCHHHHCCCCEECCHHHHHHHHH
IGAKLLKTVDSLTPLTSDIVEGAKKIGEEDKEDSTTEEALLTEGNEKAVLKYNLKAGDII
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCEE
KASDLENPMHFPKLVDSLLISLDERVLTREEVVGATLNVDTPALTPVTPDILEGFEEEVN
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHCHHHHCC
MSVDSNATVSGGTLRIKIAEGRGIDIEVPLNGNVGAGKSAKVPTVKAEKGEVVAAPAVEA
CCCCCCCEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCCEEEECCCCC
NQEVKLEEKVIRSLTRKHYKIDKIEFGPETKIEGTTLYIREGVSEDAKKVSPLVKKVEID
CCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHEEEEE
IITPDKYNTYSETIMDVQPIATKEGDNKLGTGATRVLDGVIVMVTGTDENGVQIGEFGSS
EECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCEEECCCCCC
EGILEENIMFNRPGSPDKGEIFIKTQVTIKEKTNMERPGPMAAHKATDFITQEIREAMKA
CCCCCCCEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LEDESLVVNTETFEQVRRPGKKKVVVVKEIMGQGAMHDNLILPVEPVGYLGGKPNVDLGN
CCCCCEEEEHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCC
VPVMLSPLEVLDGGIHALTCIGPASKECSRHYWREPLVMECMKDEELDLCGVIFVGSPQI
CCEEECCHHHHCCCCEEEEEECCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCCC
NSEKFYVSERMGMMVEALDVDGAFVTTEGFGNNHIDFASHVEQIGMRGIPCVAFSFCAVQ
CCCEEEHHHHHCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
GALVVGNKYMKYMIDNNKSEGGIENEVLECNTLCPEDAIRGVEMLKAAMTGEDVKKPERA
CEEEECCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCHHH
WNANVKENNLELIEKAENVKIERVLNETSIPMSEKRKEKYSTK
HCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10085076 [H]