Definition Clostridium botulinum A str. ATCC 19397, complete genome.
Accession NC_009697
Length 3,863,450

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The map label for this gene is pps [H]

Identifier: 153930898

GI number: 153930898

Start: 2638139

End: 2640616

Strand: Reverse

Name: pps [H]

Synonym: CLB_2523

Alternate gene names: 153930898

Gene position: 2640616-2638139 (Counterclockwise)

Preceding gene: 153931739

Following gene: 153932640

Centisome position: 68.35

GC content: 28.53

Gene sequence:

>2478_bases
ATGGACACTAAAAATATTATTGGTATAAAAGATGCACGGGAAAATCAAATTGATATTGTAGGAGGAAAAGGGGCTAATTT
AGGACTAATGATTTCATGTGGCATTCCTGTTCCAGATGGCTTTATTGTAACGGCTAATGCATATAAAAATTTTTTAAAAA
GTAATGGTATATTAGAAATAATAGAACAAAAAATTAGTAATATAGATAAAGAAACTTTATCTATAAAGGATAAAACTGAA
GAGATAAGAAATTTAATTTTAAAAGCTGAGTTCCCAAAGGACTTAAAAGAAGATATATTGAGTAGATTTAATAAATTTAA
GAGACCTATTCACTTAGCAGTAAGATCTTCGGCAACAGCCGAAGATTTACCGGAAGCAAGTTTTGCAGGGCAACAAGAGA
CATATTTGAATATTATGAACAAGGAAGATTTATTTGTATCAATAAAAAAGTGCTTTTCTTCTCTTTGGAGCATTAGGGCA
TTTTCATATAGAACTAATAATGGATATGATCATTTGAATGTTGGTATAGCAGTTGTTATTCAAGAAATGATAGAATCTGA
TATTTCTGGAGTTATGTTTACATCTAACCCAATAACCGCTGAAAAGGAAATAGTGATAGATGCATCTTATAATTTGGGAG
AAGCCATAGTATCAGGAAAAGTTACGCCAGATAATTATGTTCTTGATAAAAATGGAGAAGAAATAGCTTTCACTCTTGGA
AGTAAGGAAATTTCAGTTGTATATTCAGATAAAGGAACTGTGGTTGTAAATAATTCTTCTGATATAAGAGAAAGAAGATG
TCTTCATAATGAAAATTTAAGAGAATTATTTGATATGGCCTTAAAGATAGAAGGCTTATATAAAAAAACTATGGATATAG
AATGGGCAATTAAAAATAAAAAAGTATATATTCTTCAGGCACGCCCAATTACAACTATAAAGAAAAAACAGAATAAAAAT
ACCAGAAAGCAGTACTCACAAAAAGAAAAAAAGTATTTAAATAATATGATAGAACATTTTCCAGTTGCATGTTATCCACT
AGATTATGAAATTGCAATGATACTTCAAAATATGAAATTTGAACTTTTTAAGGAGGTAGGACTGAAAATAGAGAGTCCTA
TATATATGGATAATATGGGTATAATCCATATGAATAAAATTAGGTGGAAAGTAAATCCAAAAATTTTACGCTTTTTTAAA
GTTTATTATGAATATAAAAATAATGAGAAGAATGTTGAATATGGAGAAAAGATTTTAGATTCTTGTAAAAAAGAACTCAA
AGTAATAGAACAAAATATAAATGAAAATAGTTATAAATTTTCTCTTCGTGATATAGATAAATATATGAGTGATATTATTT
CAATTCATGAAAGAATTTCTTTTGCAAGGTTTAGATATTTTCTTTTCCCATCTGTTATTATTGGCGAAAAACTTGATAAA
GAACTTAAAGCTATAAATCCAAATTATACAGAGTATGATCTCTTATCAGATTTAGATTATGTAACAGTAGATATGAATAA
GGATATTGATATTTTATCAAAAACTTTATCAAGAAATGAAGAACTAATAAAGGATTTAATAAAAGGGGCAAGCTATGCAT
TTATAAAAGAAAAATATGATTATGATTCAATAGAAATAAAAGAGTTTTTTAATAAATATGGTTGGAAGTCTAATTATTGT
TGTATTCCATTCTCTTCATCTTCTTGGAATGAGGAACCAAATAGATTTATAAAACTTCTTAAAATAACCATTTCAGAGAA
TAAAGAAAATGAAGAAAATAAATCTGGAAAGTACATGGGTATATTGAAAGAGTTGGAGGAAAGTAAAGGTAAAAAAAGAT
TTAAGAAAACAATGGGAGATATTACTTATTATAGACTAGCTCATGTTAGACGTGAGGAAAGTCAATATCTTTGGGAAACA
GGATTTGGATTATTGAGGTTGCTTTTAAAACAAATAGAAGATTTGTTAAATACAAAGAAAGAAGATTTGCTATATTTGTC
TTTTCAAGAGTTAAGAAAAGTAATAGCTATTGGTAAGTTAGAGCATGAATATAGAGAATTAATTTCATATAGGAAAAATC
ACCGTAAGGAGACTGAAGAACTTTGGGAGCAGATGGCATTAGATGCCTTTTATGATAATGAGAAGAATATTTTCAGGGGA
ATAAGCGGAAGTTCAGGTGTAGTGAGAGGTAGAGTTTGTATTATAAAAAATCATACTGAATTTGATAAACTGAAAGAAGA
TAATATTCTTATATGTCCATATACTGATCCTGAGTGGACCCCTCTTTTTAAAATAGCAAAAGGAGTTGTTTCAGATACAG
GTGGAGCTTTATCGCACGCAGCTATTGTTGCACGTGAATATAATATCCCAGCGGTATTGGGTGTTGGAAATGCCACAGTA
AATTTATCGGATGGGGAATATATATTATTAGACGGAAATAATGGTATTGTAAAGCGATTATCAGAGGAAATAATATGA

Upstream 100 bases:

>100_bases
GATGCTACAGTAGATCTTTATAAATCAATATCTCTGAATGAAATAGTTGAGAATATTGAAAAGACTAAAATTGATTTAGA
AGATATAGTGGGGTGAAAAA

Downstream 100 bases:

>100_bases
CAAAGAATATAGAAGACAAACAGGGAAAATTTGAAGAACTTACTAAGACGCCAATTTCCAAATTAATATTGAAAATGGCT
ATACCGTCTATAATGGCAAT

Product: putative phosphoenolpyruvate synthase

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 825; Mature: 825

Protein sequence:

>825_residues
MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEIIEQKISNIDKETLSIKDKTE
EIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATAEDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRA
FSYRTNNGYDHLNVGIAVVIQEMIESDISGVMFTSNPITAEKEIVIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG
SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKTMDIEWAIKNKKVYILQARPITTIKKKQNKN
TRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKFELFKEVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFK
VYYEYKNNEKNVEYGEKILDSCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK
ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYDYDSIEIKEFFNKYGWKSNYC
CIPFSSSSWNEEPNRFIKLLKITISENKENEENKSGKYMGILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWET
GFGLLRLLLKQIEDLLNTKKEDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKETEELWEQMALDAFYDNEKNIFRG
ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHAAIVAREYNIPAVLGVGNATV
NLSDGEYILLDGNNGIVKRLSEEII

Sequences:

>Translated_825_residues
MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEIIEQKISNIDKETLSIKDKTE
EIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATAEDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRA
FSYRTNNGYDHLNVGIAVVIQEMIESDISGVMFTSNPITAEKEIVIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG
SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKTMDIEWAIKNKKVYILQARPITTIKKKQNKN
TRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKFELFKEVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFK
VYYEYKNNEKNVEYGEKILDSCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK
ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYDYDSIEIKEFFNKYGWKSNYC
CIPFSSSSWNEEPNRFIKLLKITISENKENEENKSGKYMGILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWET
GFGLLRLLLKQIEDLLNTKKEDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKETEELWEQMALDAFYDNEKNIFRG
ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHAAIVAREYNIPAVLGVGNATV
NLSDGEYILLDGNNGIVKRLSEEII
>Mature_825_residues
MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEIIEQKISNIDKETLSIKDKTE
EIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATAEDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRA
FSYRTNNGYDHLNVGIAVVIQEMIESDISGVMFTSNPITAEKEIVIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG
SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKTMDIEWAIKNKKVYILQARPITTIKKKQNKN
TRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKFELFKEVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFK
VYYEYKNNEKNVEYGEKILDSCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK
ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYDYDSIEIKEFFNKYGWKSNYC
CIPFSSSSWNEEPNRFIKLLKITISENKENEENKSGKYMGILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWET
GFGLLRLLLKQIEDLLNTKKEDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKETEELWEQMALDAFYDNEKNIFRG
ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHAAIVAREYNIPAVLGVGNATV
NLSDGEYILLDGNNGIVKRLSEEII

Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=356, Percent_Identity=35.6741573033708, Blast_Score=196, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17564524, Length=896, Percent_Identity=26.5625, Blast_Score=196, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 95435; Mature: 95435

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEI
CCCCCEEECCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECHHHHHHHHCCCHHHH
IEQKISNIDKETLSIKDKTEEIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATA
HHHHHHCCCHHHEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCH
EDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRAFSYRTNNGYDHLNVGIAVVI
HHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHH
QEMIESDISGVMFTSNPITAEKEIVIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG
HHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEECCCCCEEEEEEC
SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKTMDIEWAIKNK
CCEEEEEEECCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECC
KVYILQARPITTIKKKQNKNTRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKF
EEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH
ELFKEVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFKVYYEYKNNEKNVEYGEKILD
HHHHHHCCEECCCEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYD
HHHHCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEHHCCC
YDSIEIKEFFNKYGWKSNYCCIPFSSSSWNEEPNRFIKLLKITISENKENEENKSGKYMG
CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCHHHH
ILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWETGFGLLRLLLKQIEDLLNTKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKETEELWEQMALDAFYDNEKNIFRG
HHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCC
ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHA
CCCCCCEEEEEEEEEECCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHH
AIVAREYNIPAVLGVGNATVNLSDGEYILLDGNNGIVKRLSEEII
HHEEEECCCCEEEECCCEEEEECCCCEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MDTKNIIGIKDARENQIDIVGGKGANLGLMISCGIPVPDGFIVTANAYKNFLKSNGILEI
CCCCCEEECCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECHHHHHHHHCCCHHHH
IEQKISNIDKETLSIKDKTEEIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATA
HHHHHHCCCHHHEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCH
EDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRAFSYRTNNGYDHLNVGIAVVI
HHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHH
QEMIESDISGVMFTSNPITAEKEIVIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG
HHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEECCCCCEEEEEEC
SKEISVVYSDKGTVVVNNSSDIRERRCLHNENLRELFDMALKIEGLYKKTMDIEWAIKNK
CCEEEEEEECCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECC
KVYILQARPITTIKKKQNKNTRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKF
EEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH
ELFKEVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFKVYYEYKNNEKNVEYGEKILD
HHHHHHCCEECCCEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SCKKELKVIEQNINENSYKFSLRDIDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYD
HHHHCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEHHCCC
YDSIEIKEFFNKYGWKSNYCCIPFSSSSWNEEPNRFIKLLKITISENKENEENKSGKYMG
CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCHHHH
ILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWETGFGLLRLLLKQIEDLLNTKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKETEELWEQMALDAFYDNEKNIFRG
HHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCC
ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHA
CCCCCCEEEEEEEEEECCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHH
AIVAREYNIPAVLGVGNATVNLSDGEYILLDGNNGIVKRLSEEII
HHEEEECCCCEEEECCCEEEEECCCCEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.083 {pyruvate}} 0.028 {ATP}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7704256 [H]