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The map label for this gene is prfB [H]

Identifier: 152977397

GI number: 152977397

Start: 3779414

End: 3780397

Strand: Reverse

Name: prfB [H]

Synonym: Bcer98_3725

Alternate gene names: 152977397

Gene position: 3780397-3779414 (Counterclockwise)

Preceding gene: 152977398

Following gene: 152977396

Centisome position: 92.5

GC content: 37.09

Gene sequence:

>984_bases
ATGATGGAAGCTGGCTTTTGGGATGACCAACAAGGTGCTCAAACTGTTATTAATGAAGCAAATGCATTAAAAGATATGGT
TGGGAAGTTCCGTCAGCTAGATGAAACATTTGAAAATTTAGAAGTAACACATGAACTGTTAAAAGAAGAGTACGATAAAG
ATTTACATGAGGAATTGGAATCTGAAGTGAAATCTTTAATACAAGAAATGAATGAATACGAATTGCAATTGTTATTAAGT
GATCCTTATGATAAAAATAATGCCATTTTAGAATTGCACCCAGGTGCAGGTGGAACAGAGTCACAAGACTGGGGATCGAT
GTTATTGCGCATGTATACAAGATGGGCTGAAAAGCGCGGATTTAAAGTGGAAACAGTAGATTACTTACCAGGTGATGAAG
CTGGTATTAAGAGTGTGACGCTGTTAATTAAAGGTCATAATGCATACGGTTATTTAAAAGCTGAAAAAGGTGTACATCGT
CTTGTTCGTATTTCACCATTTGATTCTTCAGGTCGTCGCCATACATCATTTGTATCTTGTGAAGTTGTACCTGAATTTAA
TGATGAAGTTGAAATTGAAGTACGTACAGAAGATTTAAAGGTGGATACGTACCGTGCAAGTGGTGCTGGTGGACAGCACG
TTAATACGACAGATTCAGCAGTTCGTATTACACATATACCGACAAATACGATTGTAACTTGTCAATCGGAACGTTCACAA
ATTAAAAACCGTGAGCATGCGATGAAAATGTTAAAAGCAAAATTGTACCAAAAGAAATTAGAAGAGCAACAAGCGGAATT
AGATGAAATCCGTGGGGAACAAAAGGAAATTGGCTGGGGTAGCCAAATTCGTTCTTACGTATTTCACCCATATTCTCTTG
TTAAAGATCATCGTACAAATACAGAGGTTGGTAACGTACAAGCGGTTATGGATGGAGAAATCGACCCATTTATTGATGCA
TATTTACGTTCTCGTATTTCCTAA

Upstream 100 bases:

>100_bases
AGACAGGAATTAGAGAAAATGGCTAAGAGATTAGCGGCTTTTAGGGGGTCTCTTTGACCTTCCTGCAAAAGAAAAGCGCA
TTGCAGAATTAGAAGAAACG

Downstream 100 bases:

>100_bases
AAAGCATACTAAAAATAGTAGCGCAGATCACCACACTATGGTCTGAACTACTATTTTTTATAGTGGAGGAAAATGAAGTG
CAGTAGTGGCTAGCATACAA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MMEAGFWDDQQGAQTVINEANALKDMVGKFRQLDETFENLEVTHELLKEEYDKDLHEELESEVKSLIQEMNEYELQLLLS
DPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCEVVPEFNDEVEIEVRTEDLKVDTYRASGAGGQHVNTTDSAVRITHIPTNTIVTCQSERSQ
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVGNVQAVMDGEIDPFIDA
YLRSRIS

Sequences:

>Translated_327_residues
MMEAGFWDDQQGAQTVINEANALKDMVGKFRQLDETFENLEVTHELLKEEYDKDLHEELESEVKSLIQEMNEYELQLLLS
DPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCEVVPEFNDEVEIEVRTEDLKVDTYRASGAGGQHVNTTDSAVRITHIPTNTIVTCQSERSQ
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVGNVQAVMDGEIDPFIDA
YLRSRIS
>Mature_327_residues
MMEAGFWDDQQGAQTVINEANALKDMVGKFRQLDETFENLEVTHELLKEEYDKDLHEELESEVKSLIQEMNEYELQLLLS
DPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCEVVPEFNDEVEIEVRTEDLKVDTYRASGAGGQHVNTTDSAVRITHIPTNTIVTCQSERSQ
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVGNVQAVMDGEIDPFIDA
YLRSRIS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=305, Percent_Identity=37.3770491803279, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=35.625, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI166795305, Length=209, Percent_Identity=36.8421052631579, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI2367172, Length=318, Percent_Identity=48.4276729559748, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1787462, Length=317, Percent_Identity=36.2776025236593, Blast_Score=191, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=252, Percent_Identity=36.1111111111111, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=315, Percent_Identity=33.968253968254, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19921226, Length=260, Percent_Identity=39.2307692307692, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37417; Mature: 37417

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEAGFWDDQQGAQTVINEANALKDMVGKFRQLDETFENLEVTHELLKEEYDKDLHEELE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
SEVKSLIQEMNEYELQLLLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAEKRG
HHHHHHHHHCCHHEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC
CEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEHHHCCEEEEEECCCCCCCCCCCEEEEE
EVVPEFNDEVEIEVRTEDLKVDTYRASGAGGQHVNTTDSAVRITHIPTNTIVTCQSERSQ
EECCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECHHHHH
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHHCCCCC
TEVGNVQAVMDGEIDPFIDAYLRSRIS
CCCCCEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MMEAGFWDDQQGAQTVINEANALKDMVGKFRQLDETFENLEVTHELLKEEYDKDLHEELE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
SEVKSLIQEMNEYELQLLLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAEKRG
HHHHHHHHHCCHHEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC
CEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEHHHCCEEEEEECCCCCCCCCCCEEEEE
EVVPEFNDEVEIEVRTEDLKVDTYRASGAGGQHVNTTDSAVRITHIPTNTIVTCQSERSQ
EECCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECHHHHH
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHHCCCCC
TEVGNVQAVMDGEIDPFIDAYLRSRIS
CCCCCEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]