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The map label for this gene is sigL [H]

Identifier: 152977360

GI number: 152977360

Start: 3744642

End: 3745949

Strand: Reverse

Name: sigL [H]

Synonym: Bcer98_3685

Alternate gene names: 152977360

Gene position: 3745949-3744642 (Counterclockwise)

Preceding gene: 152977370

Following gene: 152977359

Centisome position: 91.65

GC content: 36.85

Gene sequence:

>1308_bases
TTGAAGGCAAGTCTTTTACAAGAACAAAGCTTACGTTTGGCAATGACACAAGAGTTGAGGCAAGCGATTACAATGCTCCA
GTACAATGTACAAGAATTGTCGGAGTTTTTGTATGAGCAATCGCTAGAGAATCCCCTTATTGAGTTAGGGTGTTTTGAGA
GGGAAAGAAAAAAGAGCTCTAGCAAAAGTAAAAGTACGAGCAAGCAAGTTGATAATCAAATGGAGATCTATAGTGTGGAT
TCTAAAACGATTCAGCAACATTTATTAAATCAAATACAGTATTATAAAATAGATGAAGAGCATCGGAAGACAGCTTCTTT
TATCATTATGAACCTAGATGAAAATGGGTATCTAGAAGAAACGAATGAAGAATTAGCTGAACTACTTACCGTGCCCATCG
ATGTCGTTGAACGTTCTGTAGAGATTGTCCAATCACTAGAGCCAGCAGGGGTAGGAGCGCGGAATATTCAAGAGTGTCTA
GTGATCCAATTGAAGCGTTTACAAAAGGGAAGTGTACTTGCAGAGAGGATTGTAAGTGAATATTTTTCTTATTTTGTGAG
GAAAGATTGGCGAAAACTAATCCAATTGCTGAAATGTAATAATGAAGAATTACAAGAGGCGGTTGATTGTATTACATCAT
TACAACCAAAGCCCGGACTTGCATTTTCATCTGAAAAGCCACTATATATCGTTCCTGACATGGCAGTGAAAAAAGAGGGA
GAACGGCTTATTTTACAAATGAATGAAGGAAATATGCCGAGAATTGAAATTCATTCAGAATATAGTGCACTTCTGAATAA
TAGTGAGAGTGAAGTTGCTTCTTATGTTTCTGAGAAGTATCAGCATGTGCAATGGATTATGCGCAGTTTGAAACAGCGGA
AACAAACGCTTTTACAAGTGATGGAAATGATCATGAAGAAGCAACATGATTTCTTTTGGAGAGGACCGGAACATTTAAAG
CCGCTTTCTTTGAAAGAAGTAGCGGAAGAGTTAGGTGTACATGAATCTACAATTAGTCGTGCTACACGTAACAAATATGT
ACAAACGCCATATGGGTTGTTTGAGATGAAATCATTCTTTAGTAATGCAGTCATTACAACAGAAGATGAAGCAATCTCAA
CAAAACGTGTAAAACAATTGATTCAGACGCTGATAGAAAAAGAAAATAAGAAAAAGCCACTTTCAGATCAAAAAATTTCG
AAATTGTTAGAAGAAGAGCATGAGGTTGTAATTTCAAGAAGAACGGTCGCGAAATATCGGGAACAAATGCACATTCCTGC
ATCTTCTTTGAGAAAAACAATTGGATAG

Upstream 100 bases:

>100_bases
ATTAATACATATAAATTAATAATATTTTGGAATGGGTGTCTGCGTTAGGATTCTCCTTTTACCAAACTTATTGTACAATA
GAAACAAGGGGGAGATCAGT

Downstream 100 bases:

>100_bases
GTGAAGAAGATGAAGATTATTTTATATACGAAAACAGACTGTGGTCTTTGTGTAAAGGCAAAACAAGTTTTGAATGAAGT
GCAAAAGGTGTATTCTTTTC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGCFERERKKSSSKSKSTSKQVDNQMEIYSVD
SKTIQQHLLNQIQYYKIDEEHRKTASFIIMNLDENGYLEETNEELAELLTVPIDVVERSVEIVQSLEPAGVGARNIQECL
VIQLKRLQKGSVLAERIVSEYFSYFVRKDWRKLIQLLKCNNEELQEAVDCITSLQPKPGLAFSSEKPLYIVPDMAVKKEG
ERLILQMNEGNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQVMEMIMKKQHDFFWRGPEHLK
PLSLKEVAEELGVHESTISRATRNKYVQTPYGLFEMKSFFSNAVITTEDEAISTKRVKQLIQTLIEKENKKKPLSDQKIS
KLLEEEHEVVISRRTVAKYREQMHIPASSLRKTIG

Sequences:

>Translated_435_residues
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGCFERERKKSSSKSKSTSKQVDNQMEIYSVD
SKTIQQHLLNQIQYYKIDEEHRKTASFIIMNLDENGYLEETNEELAELLTVPIDVVERSVEIVQSLEPAGVGARNIQECL
VIQLKRLQKGSVLAERIVSEYFSYFVRKDWRKLIQLLKCNNEELQEAVDCITSLQPKPGLAFSSEKPLYIVPDMAVKKEG
ERLILQMNEGNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQVMEMIMKKQHDFFWRGPEHLK
PLSLKEVAEELGVHESTISRATRNKYVQTPYGLFEMKSFFSNAVITTEDEAISTKRVKQLIQTLIEKENKKKPLSDQKIS
KLLEEEHEVVISRRTVAKYREQMHIPASSLRKTIG
>Mature_435_residues
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGCFERERKKSSSKSKSTSKQVDNQMEIYSVD
SKTIQQHLLNQIQYYKIDEEHRKTASFIIMNLDENGYLEETNEELAELLTVPIDVVERSVEIVQSLEPAGVGARNIQECL
VIQLKRLQKGSVLAERIVSEYFSYFVRKDWRKLIQLLKCNNEELQEAVDCITSLQPKPGLAFSSEKPLYIVPDMAVKKEG
ERLILQMNEGNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQVMEMIMKKQHDFFWRGPEHLK
PLSLKEVAEELGVHESTISRATRNKYVQTPYGLFEMKSFFSNAVITTEDEAISTKRVKQLIQTLIEKENKKKPLSDQKIS
KLLEEEHEVVISRRTVAKYREQMHIPASSLRKTIG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=476, Percent_Identity=33.1932773109244, Blast_Score=269, Evalue=2e-73,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 50598; Mature: 50598

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGCFERERKKSS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHH
SKSKSTSKQVDNQMEIYSVDSKTIQQHLLNQIQYYKIDEEHRKTASFIIMNLDENGYLEE
HHHHHHHHHHCCCHHEEECCHHHHHHHHHHHHHHCCCCHHHHHCEEEEEEECCCCCCCCH
TNEELAELLTVPIDVVERSVEIVQSLEPAGVGARNIQECLVIQLKRLQKGSVLAERIVSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YFSYFVRKDWRKLIQLLKCNNEELQEAVDCITSLQPKPGLAFSSEKPLYIVPDMAVKKEG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHCCC
ERLILQMNEGNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQV
CEEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEMIMKKQHDFFWRGPEHLKPLSLKEVAEELGVHESTISRATRNKYVQTPYGLFEMKSFF
HHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
SNAVITTEDEAISTKRVKQLIQTLIEKENKKKPLSDQKISKLLEEEHEVVISRRTVAKYR
CCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQMHIPASSLRKTIG
HHHCCCHHHHHHHCC
>Mature Secondary Structure
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGCFERERKKSS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHH
SKSKSTSKQVDNQMEIYSVDSKTIQQHLLNQIQYYKIDEEHRKTASFIIMNLDENGYLEE
HHHHHHHHHHCCCHHEEECCHHHHHHHHHHHHHHCCCCHHHHHCEEEEEEECCCCCCCCH
TNEELAELLTVPIDVVERSVEIVQSLEPAGVGARNIQECLVIQLKRLQKGSVLAERIVSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YFSYFVRKDWRKLIQLLKCNNEELQEAVDCITSLQPKPGLAFSSEKPLYIVPDMAVKKEG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHCCC
ERLILQMNEGNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQV
CEEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEMIMKKQHDFFWRGPEHLKPLSLKEVAEELGVHESTISRATRNKYVQTPYGLFEMKSFF
HHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
SNAVITTEDEAISTKRVKQLIQTLIEKENKKKPLSDQKISKLLEEEHEVVISRRTVAKYR
CCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQMHIPASSLRKTIG
HHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377 [H]