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The map label for this gene is pgk [H]

Identifier: 152977356

GI number: 152977356

Start: 3740871

End: 3742055

Strand: Reverse

Name: pgk [H]

Synonym: Bcer98_3681

Alternate gene names: 152977356

Gene position: 3742055-3740871 (Counterclockwise)

Preceding gene: 152977357

Following gene: 152977355

Centisome position: 91.56

GC content: 40.42

Gene sequence:

>1185_bases
ATGAACAAAAAATCAATTCGTGACGTAGATTTAAAGGGTAAACGTGTATTTTGCCGCGTTGATTTCAACGTACCTATGAA
AGATGGCAAAATTACAGACGAGACTCGTATTCGTGCAGCTCTTCCTACTATTCAATATTTAGTAGAACAAGGTGCGAAGG
TTATTTTAGCAAGCCACTTAGGCCGTCCAAAAGGTCAAGTAGTAGAAGAAATGCGTTTAACTCCAGTAGCAGTTCGTTTA
GGTGAACTTCTTGGTAAAGACGTGAAAAAAGCGGACGAAGCATTCGGACCAGCTGTACAAGAAATGGTTGCAGCAATGAA
CGAAGGCGACATTTTAGTTCTTGAAAACGTTCGTTTCTATGCGGGCGAAGAAAAGAACGATGCAGAACTTGCGAAAGAAT
TTGCAGCTCTTGCTGATATCTTCGTAAACGATGCATTCGGTGCAGCTCACCGCGCTCATGCTTCTACAGCAGGGATTGCA
GATTACCTACCAGCAGTAGCAGGTTTCTTAATGGAAAAAGAATTAGAAGTACTTGGAAAAGCACTTTCTAATCCAGATCG
TCCATTTACAGCAATCATCGGTGGTGCGAAAGTAAAAGATAAAATCGGTGTAATTCGTCATCTATTAGATAAAGTAGATA
ACTTAATCATCGGTGGAGGTCTTGCTTACACATTCGTAAAAGCATTAGGTCATGAAATTGGTCTATCTCTATGTGAAGAT
GACAAGATTGAACTAGCAAAAGAGTTTATGGCACTTGCAAAAGAAAAAGGTGTAAACTTCTACATGCCAGTTGATGTTGT
AATCACTGAGGAATTCTCTGAAACTGCAACAACTAAAATTGTAGATATCGACTCTATCCCATCAAACTGGGAAGGTGTGG
ACATCGGACCAAAAACACGTGAAATTTATGCAGATGTAATTAAAAACTCTAAGCTTGTTGTATGGAATGGACCAATGGGT
GTATTCGAAATGACTCCATTCTCTAACGGTACAAGAGCAGTAGGACAAGCATTAGCAGATGCAGAAGGTACATACTCTGT
TATCGGCGGTGGTGACTCTGCAGCAGCTGTTGAAAAATTCGGCATGGCTGATAAAATGAGCCACATTTCTACTGGCGGCG
GTGCGTCATTAGAATTTATGGAAGGTAAAGAACTTCCAGGTGTAGTTTGTCTGAATGACAAATAA

Upstream 100 bases:

>100_bases
GGGAGAGGGTTTGTTCCCTCTCTCTTCTTTCGTTTTATAAGCAAATGTGATAAGCTTTTGAATGGGTTTTGTCAATCCTA
ACTAGTCGGAGGGAAATCCA

Downstream 100 bases:

>100_bases
TTAGCAGCCAAAGAAAAAAGGACGGTGCAAAGCATGCGTAAACCAATTATCGCAGGTAACTGGAAAATGAATAAAACTCT
ATCTGAAGCAGTTAGCTTCG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKDGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQVVEEMRLTPVAVRL
GELLGKDVKKADEAFGPAVQEMVAAMNEGDILVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVAGFLMEKELEVLGKALSNPDRPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMALAKEKGVNFYMPVDVVITEEFSETATTKIVDIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFSNGTRAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK

Sequences:

>Translated_394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKDGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQVVEEMRLTPVAVRL
GELLGKDVKKADEAFGPAVQEMVAAMNEGDILVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVAGFLMEKELEVLGKALSNPDRPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMALAKEKGVNFYMPVDVVITEEFSETATTKIVDIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFSNGTRAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
>Mature_394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKDGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQVVEEMRLTPVAVRL
GELLGKDVKKADEAFGPAVQEMVAAMNEGDILVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVAGFLMEKELEVLGKALSNPDRPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMALAKEKGVNFYMPVDVVITEEFSETATTKIVDIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFSNGTRAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=47.1014492753623, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=46.7312348668281, Blast_Score=348, Evalue=4e-96,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=46.9849246231156, Blast_Score=346, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=47.8155339805825, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=49.7572815533981, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=46.4720194647202, Blast_Score=338, Evalue=5e-93,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=46.0240963855422, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42392; Mature: 42392

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSIRDVDLKGKRVFCRVDFNVPMKDGKITDETRIRAALPTIQYLVEQGAKVILASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHC
GRPKGQVVEEMRLTPVAVRLGELLGKDVKKADEAFGPAVQEMVAAMNEGDILVLENVRFY
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEEEE
AGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIADYLPAVAGFLMEKELEVLGK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALSNPDRPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCC
DKIELAKEFMALAKEKGVNFYMPVDVVITEEFSETATTKIVDIDSIPSNWEGVDIGPKTR
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHCCHHEEEEEECCCCCCCCCCCCCCCHH
EIYADVIKNSKLVVWNGPMGVFEMTPFSNGTRAVGQALADAEGTYSVIGGGDSAAAVEKF
HHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
GMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCC
>Mature Secondary Structure
MNKKSIRDVDLKGKRVFCRVDFNVPMKDGKITDETRIRAALPTIQYLVEQGAKVILASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHC
GRPKGQVVEEMRLTPVAVRLGELLGKDVKKADEAFGPAVQEMVAAMNEGDILVLENVRFY
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEEEE
AGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIADYLPAVAGFLMEKELEVLGK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALSNPDRPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCC
DKIELAKEFMALAKEKGVNFYMPVDVVITEEFSETATTKIVDIDSIPSNWEGVDIGPKTR
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHCCHHEEEEEECCCCCCCCCCCCCCCHH
EIYADVIKNSKLVVWNGPMGVFEMTPFSNGTRAVGQALADAEGTYSVIGGGDSAAAVEKF
HHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
GMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA