Click here to switch to the map view.

The map label for this gene is glnT [H]

Identifier: 152977317

GI number: 152977317

Start: 3701540

End: 3702982

Strand: Reverse

Name: glnT [H]

Synonym: Bcer98_3642

Alternate gene names: 152977317

Gene position: 3702982-3701540 (Counterclockwise)

Preceding gene: 152977319

Following gene: 152977316

Centisome position: 90.6

GC content: 36.8

Gene sequence:

>1443_bases
ATGGAAATGTTAGCGAGATTGATTACGGATATAAATAATGTTCTTTGGACATATATTATGATAGGTATGTTAATTAGTAT
TGGCATTTATTTTTCTTTACGAGTTAATTTTGTTCAAATTCGCTATTTTGGTGAAATGATTCGCTTACTAGGAGATGGAG
CGAGTTCGAAAACACGTAAAGCACAAAAGGAAAAGAATGGCGTTTCGTCATTCCAAGCGTTTTGTATGAGTACAGCTTCT
CGTGTAGGTACAGGTAATTTAGCAGGTGTGGCAATTGCGATTTCAACTGGGGGACCTGGGGCTGTATTTTGGATGTGGCT
GATTGCGATAATTGGTGGTGCATCTGCTTTTGTAGAAAGTACATTAGCGCAAATTTACAAAGTGAAAGATGGAAATACGT
TCCGCGGGGGACCGGCGTATTATATGGAAAAAGGACTAAATAAGCGCTGGCTCGGTGCTATTTTCTCTATTTTAATCACA
GTTAGTTTTGGTTTGATTTTTAATGCGGTACAGTCAAATACTGTGGCAGCAGCATTTGATGGCGCATTTCATACAGATAG
TAGATTAGTCGGCATTGTAATGGCTGGACTGTTAGCAATTATTATTTTTGGTGGGGTAAAACGAATTGCTCGCGCTGTCG
AAATGATTGTTCCTGTTATGGCGCTTATTTATATAGGAGTTGCCTTGTTCGTAGTGATTACAAATATTACAGAGATTCCA
ACTTTATTTAAAGAAATTATTTTACATGCTTTTGGGATTAAAGAAGTTGTTGGTGGTGGATTTGGAGCGGCTATTTTACT
AGGGGTAAAACGTGGATTGTTTTCAAATGAAGCTGGTATGGGGAGCGCGCCAAACGCAGCAGCAACTGCGAATGTAACAC
ATCCCGTCAAACAAGGGTTTATCCAAACATTAGGGGTATTTACAGATACATTGCTAATTTGTAGTTGTACAGCATTTATT
ATTTTGTTATCAGATGTATCAGGATTAGCTAATACAAATGGTATCCAATTAACACAACAAGCATTAAGTCAACATATTGG
ATCTTGGGCCTCTATTTTTGTAGCAGTTGCAATTTTCTTATTTGCATTTAGTTCACTTGTTGGGAATTACTATTATGGTG
AAACAAATATTGAATTCTTAAATGGAAGTAAAGGGATATTGATCGTTTACCGAATTGCAGTGCTTGGTATGGTTATACTT
GGTTCGGTAGCTACAATTCAAATTGTATGGGATTTAGCAGACCTATTTATGGGGATTATGGCTGTTATTAATTTAGTGGC
AATTGTATTTCTATCTAAGTTTGCTTTTACAGCATTATCAGACTATGTAAAACAAAAGAAACAAGGAAGAGACCCAGTGT
TTCATGCAAGTTCGATTCCGGGGCTAGAAAATACAGAGTGCTGGGAAGATTCTAAAGTAGAAGAAAAAGAACAAACTATG
TAA

Upstream 100 bases:

>100_bases
TTGTGTCATAAAACCTAACATTTTTTTGTCTAAACTTGTTGATTCTTAAAATTTTTTAGAATATAATCTTCTTATTGTTA
AGAGAGGGGGAAGTGATTCG

Downstream 100 bases:

>100_bases
AAAGGAGGTATCAGAGAAAATCTGATACCTTTTTTATATCTTTATAAAAGACGAACATTAATAATTTTTTTATATTCATT
GTAATTTTTTTCGGAAATAT

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MEMLARLITDINNVLWTYIMIGMLISIGIYFSLRVNFVQIRYFGEMIRLLGDGASSKTRKAQKEKNGVSSFQAFCMSTAS
RVGTGNLAGVAIAISTGGPGAVFWMWLIAIIGGASAFVESTLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSILIT
VSFGLIFNAVQSNTVAAAFDGAFHTDSRLVGIVMAGLLAIIIFGGVKRIARAVEMIVPVMALIYIGVALFVVITNITEIP
TLFKEIILHAFGIKEVVGGGFGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGFIQTLGVFTDTLLICSCTAFI
ILLSDVSGLANTNGIQLTQQALSQHIGSWASIFVAVAIFLFAFSSLVGNYYYGETNIEFLNGSKGILIVYRIAVLGMVIL
GSVATIQIVWDLADLFMGIMAVINLVAIVFLSKFAFTALSDYVKQKKQGRDPVFHASSIPGLENTECWEDSKVEEKEQTM

Sequences:

>Translated_480_residues
MEMLARLITDINNVLWTYIMIGMLISIGIYFSLRVNFVQIRYFGEMIRLLGDGASSKTRKAQKEKNGVSSFQAFCMSTAS
RVGTGNLAGVAIAISTGGPGAVFWMWLIAIIGGASAFVESTLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSILIT
VSFGLIFNAVQSNTVAAAFDGAFHTDSRLVGIVMAGLLAIIIFGGVKRIARAVEMIVPVMALIYIGVALFVVITNITEIP
TLFKEIILHAFGIKEVVGGGFGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGFIQTLGVFTDTLLICSCTAFI
ILLSDVSGLANTNGIQLTQQALSQHIGSWASIFVAVAIFLFAFSSLVGNYYYGETNIEFLNGSKGILIVYRIAVLGMVIL
GSVATIQIVWDLADLFMGIMAVINLVAIVFLSKFAFTALSDYVKQKKQGRDPVFHASSIPGLENTECWEDSKVEEKEQTM
>Mature_480_residues
MEMLARLITDINNVLWTYIMIGMLISIGIYFSLRVNFVQIRYFGEMIRLLGDGASSKTRKAQKEKNGVSSFQAFCMSTAS
RVGTGNLAGVAIAISTGGPGAVFWMWLIAIIGGASAFVESTLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSILIT
VSFGLIFNAVQSNTVAAAFDGAFHTDSRLVGIVMAGLLAIIIFGGVKRIARAVEMIVPVMALIYIGVALFVVITNITEIP
TLFKEIILHAFGIKEVVGGGFGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGFIQTLGVFTDTLLICSCTAFI
ILLSDVSGLANTNGIQLTQQALSQHIGSWASIFVAVAIFLFAFSSLVGNYYYGETNIEFLNGSKGILIVYRIAVLGMVIL
GSVATIQIVWDLADLFMGIMAVINLVAIVFLSKFAFTALSDYVKQKKQGRDPVFHASSIPGLENTECWEDSKVEEKEQTM

Specific function: Probably functions as a sodium/glutamine symporter for glutamine uptake [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=477, Percent_Identity=42.3480083857442, Blast_Score=353, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51629; Mature: 51629

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLARLITDINNVLWTYIMIGMLISIGIYFSLRVNFVQIRYFGEMIRLLGDGASSKTRK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHHH
AQKEKNGVSSFQAFCMSTASRVGTGNLAGVAIAISTGGPGAVFWMWLIAIIGGASAFVES
HHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHH
TLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSILITVSFGLIFNAVQSNTVAAAFD
HHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GAFHTDSRLVGIVMAGLLAIIIFGGVKRIARAVEMIVPVMALIYIGVALFVVITNITEIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLFKEIILHAFGIKEVVGGGFGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
IQTLGVFTDTLLICSCTAFIILLSDVSGLANTNGIQLTQQALSQHIGSWASIFVAVAIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FAFSSLVGNYYYGETNIEFLNGSKGILIVYRIAVLGMVILGSVATIQIVWDLADLFMGIM
HHHHHHHCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVINLVAIVFLSKFAFTALSDYVKQKKQGRDPVFHASSIPGLENTECWEDSKVEEKEQTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MEMLARLITDINNVLWTYIMIGMLISIGIYFSLRVNFVQIRYFGEMIRLLGDGASSKTRK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHHH
AQKEKNGVSSFQAFCMSTASRVGTGNLAGVAIAISTGGPGAVFWMWLIAIIGGASAFVES
HHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHH
TLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSILITVSFGLIFNAVQSNTVAAAFD
HHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GAFHTDSRLVGIVMAGLLAIIIFGGVKRIARAVEMIVPVMALIYIGVALFVVITNITEIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLFKEIILHAFGIKEVVGGGFGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
IQTLGVFTDTLLICSCTAFIILLSDVSGLANTNGIQLTQQALSQHIGSWASIFVAVAIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FAFSSLVGNYYYGETNIEFLNGSKGILIVYRIAVLGMVILGSVATIQIVWDLADLFMGIM
HHHHHHHCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVINLVAIVFLSKFAFTALSDYVKQKKQGRDPVFHASSIPGLENTECWEDSKVEEKEQTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]