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The map label for this gene is ldh [H]

Identifier: 152977270

GI number: 152977270

Start: 3648376

End: 3649326

Strand: Reverse

Name: ldh [H]

Synonym: Bcer98_3591

Alternate gene names: 152977270

Gene position: 3649326-3648376 (Counterclockwise)

Preceding gene: 152977271

Following gene: 152977269

Centisome position: 89.29

GC content: 35.33

Gene sequence:

>951_bases
ATGAATAGAAACACAAGAAAAATCGCAATTATTGGAACTGGATTAGTTGGGTCAAGCTGTGCGTATTCAATTGTGAATCA
AGGCATCTGTGAAGAATTAGCATTAATTGATATAGACCATGAACGTGCAGTTGGTGAAGCGATGGATTTATCACACTGTA
TTAACTTTACAAATACAAGAACAAAAGTATATGCAGGTACGTATGAAAACTGCAAAGACATGGACATTGTTATTATTACA
GCAGGTCCAGCGCCAAAGCCTGGGCAAAGTCGATTAGATACTTTAGGGGCAAGTGCAAAAATTATGGAAAGTGTTGTAGC
TTGTGTAATGAAAAGTGGATTTGATGGCATTTTCTTAATTGCTTCAAACCCAGTTGATATTATTACGTATCAAGTTTGGA
AATTATCTGGTTTACCAAGAAATCGTGTGATTGGTACTGGAACATCGTTAGATTCCTCTCGATTAAGAACGATTTTATCT
GAAATGTTAGAAGTGGATCCTCGTAGTATTCACGGTTATTCATTAGGGGAACATGGTGATTCTCAAATGGTTGCTTGGTC
TCATGTAACAGTGGGAGGGAAACCTATTTTACAAATTTTAGAGGAAGGGAAAGATCGTTTTGGTGAAATTGACTTAGATG
CAATTGTGGAAAAAACTGCAAAAGCTGGTTGGGAAATTTATAAACGAAAAGGAACTACTTATTATGGTATTGGCAACTCA
CTCGCATATATTGTAAAATCTATCTTTAATGATGATTATCGTGTAATCGCCGTTTCAGCTATTTTAGATGGTGAATATGG
TGAGTATGATATTTGTACAGGGGTACCAGCGATTATTACAAGAGATGGTGTGAAAGAAGTTGTTGAACTAAACTTAACAG
AAACTGAAGAAAGTCGATTTGCAAAGTCAAATGATATTTTACGTGATTATATGAAAACAATTGGTTACTAA

Upstream 100 bases:

>100_bases
AGAATGCTCAAAATTTCACAATGTATCCATGTTTTCTATATTTGAAATCTTTATAATAATAAGTGAAATTAGATTACCGA
TTCAATTAGGAGAGATTATT

Downstream 100 bases:

>100_bases
ACTAGGAGAAGGTGAAACAGATGAAGGAATATATTATGTCTCGTGTTTTTAAAGGTTCTGCCGGAATTGCACAAGGTATT
TTTGTATCTCTCGGAATTGG

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 3 [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNRNTRKIAIIGTGLVGSSCAYSIVNQGICEELALIDIDHERAVGEAMDLSHCINFTNTRTKVYAGTYENCKDMDIVIIT
AGPAPKPGQSRLDTLGASAKIMESVVACVMKSGFDGIFLIASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILS
EMLEVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEGKDRFGEIDLDAIVEKTAKAGWEIYKRKGTTYYGIGNS
LAYIVKSIFNDDYRVIAVSAILDGEYGEYDICTGVPAIITRDGVKEVVELNLTETEESRFAKSNDILRDYMKTIGY

Sequences:

>Translated_316_residues
MNRNTRKIAIIGTGLVGSSCAYSIVNQGICEELALIDIDHERAVGEAMDLSHCINFTNTRTKVYAGTYENCKDMDIVIIT
AGPAPKPGQSRLDTLGASAKIMESVVACVMKSGFDGIFLIASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILS
EMLEVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEGKDRFGEIDLDAIVEKTAKAGWEIYKRKGTTYYGIGNS
LAYIVKSIFNDDYRVIAVSAILDGEYGEYDICTGVPAIITRDGVKEVVELNLTETEESRFAKSNDILRDYMKTIGY
>Mature_316_residues
MNRNTRKIAIIGTGLVGSSCAYSIVNQGICEELALIDIDHERAVGEAMDLSHCINFTNTRTKVYAGTYENCKDMDIVIIT
AGPAPKPGQSRLDTLGASAKIMESVVACVMKSGFDGIFLIASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILS
EMLEVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEGKDRFGEIDLDAIVEKTAKAGWEIYKRKGTTYYGIGNS
LAYIVKSIFNDDYRVIAVSAILDGEYGEYDICTGVPAIITRDGVKEVVELNLTETEESRFAKSNDILRDYMKTIGY

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=38.1410256410256, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=38.1410256410256, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=39.297124600639, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=39.297124600639, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI291575128, Length=312, Percent_Identity=36.2179487179487, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI4557032, Length=312, Percent_Identity=36.2179487179487, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI47059044, Length=310, Percent_Identity=38.0645161290323, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI221136809, Length=310, Percent_Identity=38.0645161290323, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI15082234, Length=305, Percent_Identity=37.3770491803279, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI260099727, Length=187, Percent_Identity=43.8502673796791, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI260099725, Length=187, Percent_Identity=43.8502673796791, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI207028494, Length=199, Percent_Identity=39.1959798994975, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI103472011, Length=294, Percent_Identity=29.9319727891156, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI103472015, Length=186, Percent_Identity=32.258064516129, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI21735621, Length=239, Percent_Identity=28.4518828451883, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1789632, Length=296, Percent_Identity=27.027027027027, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17535107, Length=298, Percent_Identity=36.241610738255, Blast_Score=193, Evalue=8e-50,
Organism=Drosophila melanogaster, GI17136226, Length=317, Percent_Identity=36.5930599369085, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI45550422, Length=306, Percent_Identity=31.3725490196078, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24647881, Length=240, Percent_Identity=28.75, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24663599, Length=315, Percent_Identity=26.3492063492064, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34639; Mature: 34639

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRNTRKIAIIGTGLVGSSCAYSIVNQGICEELALIDIDHERAVGEAMDLSHCINFTNTR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCCC
TKVYAGTYENCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVACVMKSGFDGIFLI
EEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEE
ASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLEVDPRSIHGYSLGEHGD
ECCCCEEEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SQMVAWSHVTVGGKPILQILEEGKDRFGEIDLDAIVEKTAKAGWEIYKRKGTTYYGIGNS
CCEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHH
LAYIVKSIFNDDYRVIAVSAILDGEYGEYDICTGVPAIITRDGVKEVVELNLTETEESRF
HHHHHHHHHCCCEEEEEEEEEECCCCCCEEHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
AKSNDILRDYMKTIGY
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRNTRKIAIIGTGLVGSSCAYSIVNQGICEELALIDIDHERAVGEAMDLSHCINFTNTR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCCC
TKVYAGTYENCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVACVMKSGFDGIFLI
EEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEE
ASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLEVDPRSIHGYSLGEHGD
ECCCCEEEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SQMVAWSHVTVGGKPILQILEEGKDRFGEIDLDAIVEKTAKAGWEIYKRKGTTYYGIGNS
CCEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHH
LAYIVKSIFNDDYRVIAVSAILDGEYGEYDICTGVPAIITRDGVKEVVELNLTETEESRF
HHHHHHHHHCCCEEEEEEEEEECCCCCCEEHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
AKSNDILRDYMKTIGY
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]