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The map label for this gene is dnaB [H]

Identifier: 152976962

GI number: 152976962

Start: 3331624

End: 3333024

Strand: Reverse

Name: dnaB [H]

Synonym: Bcer98_3265

Alternate gene names: 152976962

Gene position: 3333024-3331624 (Counterclockwise)

Preceding gene: 152976963

Following gene: 152976961

Centisome position: 81.55

GC content: 35.69

Gene sequence:

>1401_bases
ATGGAAAAACAGTCATGGATGGAGCTATTGCCAATTGATCGTTATAAAGTAAGCGCAAAAGGGTTGTTGCATAGCTATGA
CCGGAAAGTATTAACCATGCTATACCAACCTTTAGTAGGTAGCAAAGCTTTTAGTTTATACATGACACTATGGGGGGAGT
TGGAACAAGATCGCTTGTTTGGTAAAGAGAATACGCACCACTCTCTTATGGTAACCATGCAAATGCAGCTTCCTGAAATA
TATGAGGAACGAAGAAAGCTAGAAGCAATTGGGCTTTTAAATGTCTATATCAAAAAGGAAAAAGACATTCGTATGTTTAT
ATATGAACTGCAACCACCATTATCTCCAAAACAGTTTTTTGATGATATTGTTTTAAGTATTTTCTTATACAATAGGTTAA
GTAGAACGAAGTACAACCAAGTAAAAAAATATTTCTTAGAAGAAACGTTTGATTTTACTTCATATGAGAATATTACGCAT
TCTTTTAATGATGTATTTGGTTCGTTTAATCCGGAACAGTTTGAACATGTACAAGAAGAACTTCGTATTCCAAAGGTAAC
AGTGATGCCGAAAAATGAAACAGGAAATGCACCTGCAATTTGGAATGATTTCTTTGATTTTTCACTATTTGTTGAAGGAT
TATCGGCGCTTGTTCCTAAAAAATCAATTACAGAACAAGTTCGTGAATGCGTGATTACACTAGCTTATGTATACGGAGTG
GATGTGTTATCCATGCAAAATATTGTTCTTGGTGCAATGACGGAAATGCAAACAATTGATATGGAACGTCTTCGTAAAGG
GGCTCGTGATTGGTATCAATTTGAACATGGACAAGCACTTCCGGTATTAAGTGAACGTGTACAACCTCATTCAGCACGAA
CAATGGCGGAGAAAGAACCTTCTACGCAAGAAGAGTTGTTAATAAAACAATTAGAAGAAATTTCACCAAAGCAGTTATTA
AAAGAAATTTCAGGTGGCGCAGAGGCAACAAAGGCAGATTTACAAATTGTAGAAGATGTTATGTTGAATCAAAAATTAGC
ACCGGGAGTTGTGAATGTTCTTATTTATTATGTAATGTTGCGTTCTGATATGAAACTTGCTAAAACATATGTTGAAAAGA
TTGCAGGTCATTGGGCAAGGAAAAAAGTAAGCACAGTATCAGAAGCGATGGCTTTAGCGAAAGAAGAAAATCGTCAATAT
CAAGAATGGGCGGAGACAAAGAAAAAGGGACGTCCATCGACAAAAACAGTACGAAAAGAAATGGTACCGGATTGGCTGAA
AGCAGAATCAAAAGAATCATCGTCAGATAAGAAAAAGGCAAGTTCAAAACGAAAAACGAACAAGCTAGAAGAAGAACGAA
AGAGATTAGAAGAAGAATTGAAAAAATATAAGCGTGATTAA

Upstream 100 bases:

>100_bases
TAGAAGCGTAAGCTAATAGCTCTTTTTCACTAAGAAGTTCTATAAGGTGAACACATGGTAGATGTTAGGGTAGAATGGAT
GAAAGAAAGGAATTTCAAGG

Downstream 100 bases:

>100_bases
TAGAGGAGAATGAACGCTCTTTGAGGTGAAAGAATGGAGCATATTCAAAATTCATTTGCAAAATTAATGCAAAATGAAAA
CTTTAAAAATAGATATGAAA

Product: replication initiation and membrane attachment family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MEKQSWMELLPIDRYKVSAKGLLHSYDRKVLTMLYQPLVGSKAFSLYMTLWGELEQDRLFGKENTHHSLMVTMQMQLPEI
YEERRKLEAIGLLNVYIKKEKDIRMFIYELQPPLSPKQFFDDIVLSIFLYNRLSRTKYNQVKKYFLEETFDFTSYENITH
SFNDVFGSFNPEQFEHVQEELRIPKVTVMPKNETGNAPAIWNDFFDFSLFVEGLSALVPKKSITEQVRECVITLAYVYGV
DVLSMQNIVLGAMTEMQTIDMERLRKGARDWYQFEHGQALPVLSERVQPHSARTMAEKEPSTQEELLIKQLEEISPKQLL
KEISGGAEATKADLQIVEDVMLNQKLAPGVVNVLIYYVMLRSDMKLAKTYVEKIAGHWARKKVSTVSEAMALAKEENRQY
QEWAETKKKGRPSTKTVRKEMVPDWLKAESKESSSDKKKASSKRKTNKLEEERKRLEEELKKYKRD

Sequences:

>Translated_466_residues
MEKQSWMELLPIDRYKVSAKGLLHSYDRKVLTMLYQPLVGSKAFSLYMTLWGELEQDRLFGKENTHHSLMVTMQMQLPEI
YEERRKLEAIGLLNVYIKKEKDIRMFIYELQPPLSPKQFFDDIVLSIFLYNRLSRTKYNQVKKYFLEETFDFTSYENITH
SFNDVFGSFNPEQFEHVQEELRIPKVTVMPKNETGNAPAIWNDFFDFSLFVEGLSALVPKKSITEQVRECVITLAYVYGV
DVLSMQNIVLGAMTEMQTIDMERLRKGARDWYQFEHGQALPVLSERVQPHSARTMAEKEPSTQEELLIKQLEEISPKQLL
KEISGGAEATKADLQIVEDVMLNQKLAPGVVNVLIYYVMLRSDMKLAKTYVEKIAGHWARKKVSTVSEAMALAKEENRQY
QEWAETKKKGRPSTKTVRKEMVPDWLKAESKESSSDKKKASSKRKTNKLEEERKRLEEELKKYKRD
>Mature_466_residues
MEKQSWMELLPIDRYKVSAKGLLHSYDRKVLTMLYQPLVGSKAFSLYMTLWGELEQDRLFGKENTHHSLMVTMQMQLPEI
YEERRKLEAIGLLNVYIKKEKDIRMFIYELQPPLSPKQFFDDIVLSIFLYNRLSRTKYNQVKKYFLEETFDFTSYENITH
SFNDVFGSFNPEQFEHVQEELRIPKVTVMPKNETGNAPAIWNDFFDFSLFVEGLSALVPKKSITEQVRECVITLAYVYGV
DVLSMQNIVLGAMTEMQTIDMERLRKGARDWYQFEHGQALPVLSERVQPHSARTMAEKEPSTQEELLIKQLEEISPKQLL
KEISGGAEATKADLQIVEDVMLNQKLAPGVVNVLIYYVMLRSDMKLAKTYVEKIAGHWARKKVSTVSEAMALAKEENRQY
QEWAETKKKGRPSTKTVRKEMVPDWLKAESKESSSDKKKASSKRKTNKLEEERKRLEEELKKYKRD

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 [H]

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006343 [H]

Pfam domain/function: PF07261 DnaB_2 [H]

EC number: NA

Molecular weight: Translated: 54467; Mature: 54467

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQSWMELLPIDRYKVSAKGLLHSYDRKVLTMLYQPLVGSKAFSLYMTLWGELEQDRLF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
GKENTHHSLMVTMQMQLPEIYEERRKLEAIGLLNVYIKKEKDIRMFIYELQPPLSPKQFF
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCHHHHH
DDIVLSIFLYNRLSRTKYNQVKKYFLEETFDFTSYENITHSFNDVFGSFNPEQFEHVQEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LRIPKVTVMPKNETGNAPAIWNDFFDFSLFVEGLSALVPKKSITEQVRECVITLAYVYGV
HCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DVLSMQNIVLGAMTEMQTIDMERLRKGARDWYQFEHGQALPVLSERVQPHSARTMAEKEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCC
STQEELLIKQLEEISPKQLLKEISGGAEATKADLQIVEDVMLNQKLAPGVVNVLIYYVML
CHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RSDMKLAKTYVEKIAGHWARKKVSTVSEAMALAKEENRQYQEWAETKKKGRPSTKTVRKE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MVPDWLKAESKESSSDKKKASSKRKTNKLEEERKRLEEELKKYKRD
HCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKQSWMELLPIDRYKVSAKGLLHSYDRKVLTMLYQPLVGSKAFSLYMTLWGELEQDRLF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
GKENTHHSLMVTMQMQLPEIYEERRKLEAIGLLNVYIKKEKDIRMFIYELQPPLSPKQFF
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCHHHHH
DDIVLSIFLYNRLSRTKYNQVKKYFLEETFDFTSYENITHSFNDVFGSFNPEQFEHVQEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LRIPKVTVMPKNETGNAPAIWNDFFDFSLFVEGLSALVPKKSITEQVRECVITLAYVYGV
HCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DVLSMQNIVLGAMTEMQTIDMERLRKGARDWYQFEHGQALPVLSERVQPHSARTMAEKEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCC
STQEELLIKQLEEISPKQLLKEISGGAEATKADLQIVEDVMLNQKLAPGVVNVLIYYVML
CHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RSDMKLAKTYVEKIAGHWARKKVSTVSEAMALAKEENRQYQEWAETKKKGRPSTKTVRKE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MVPDWLKAESKESSSDKKKASSKRKTNKLEEERKRLEEELKKYKRD
HCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377 [H]