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The map label for this gene is asnC [H]

Identifier: 152976944

GI number: 152976944

Start: 3311847

End: 3313238

Strand: Reverse

Name: asnC [H]

Synonym: Bcer98_3247

Alternate gene names: 152976944

Gene position: 3313238-3311847 (Counterclockwise)

Preceding gene: 152976945

Following gene: 152976943

Centisome position: 81.07

GC content: 38.86

Gene sequence:

>1392_bases
ATGGAAAACACATTAGTAAAAAGTCTGTACAGAGAAACAGATAAATATGTAGATCAAAAAGTGCAAGTATCTGGTTGGAT
TCGTAACTTACGTGATTCAAAAGTATTTGGCTTTATCGAATTAAATGATGGTAGCTTCTTTAAAAGTGTACAAATCGTTT
TTGATACAGAATTAGATAACTTTAAGGAAATTACAAAATTACCGCTTAGCTCCTCTATTAGAGTAGAAGGGAAGTTCATT
GCAACACCTACTGCAAAACAACCATTTGAAATTAAAGCAGAAAAAATCGAAATTGAAGGCTTATCAGATTCTGATTACCC
ACTTCAAAAGAAACGCCATACGTTTGAATACTTACGTACAATCGCTCACTTACGTCCAAGAACAAATGCATTCTCTGCAA
CGTTCCGTGTGCGTTCTATCGCAGCTTACGCGATTCATAAGTTCTTCCAGGAGCGCGGCTTTGTCCATGTTCATACACCA
ATTATTACTGGTAGCGATACAGAAGGTGCAGGTGAAATGTTCCGCGTAACAACACATGACATGAACAACCTACCAAAAGG
AGAAGATGGACAAGTAGACGATTCGAAAGACTTCTTCGGTAGAGAAACAAACTTAACAGTAAGTGGTCAACTTCCTGCTG
AAGCTTATGCATTAGCGTTCCGTGATGTATATACATTCGGACCTACATTCCGTGCGGAAAACTCAAACACAACTCGTCAT
GCAGCTGAGTTCTGGATGGTGGAGCCGGAAATTGCATTTGCTGAACTGGAAGATGTAATGGATCTTACAGAAGATATGCT
AAAATATGCAATGAAATACGTGTTAGAGCATGCACCAGAAGAAATGGAATTCTTCAACAAATTTGTTGATAAAACAGTTC
TTGAGCGCATGAACAATGTCATTAACTCTGACTTTGGTCGCATCACATACACAGAAGCAATTAAGGTACTTCAAGAATCA
GGTGCTGACTTCAAATACCCAGTAGAATGGGGCATTGATTTACAAACAGAGCATGAAAGATATTTATCAGAACAAGTCTT
TAAACGTCCTGTATTCGTAACAGACTATCCAAAAGATATTAAAGCATTCTACATGCGTATGAACGATGATGGTAAAACAG
TAGCTGCTACTGATCTTCTTGTTCCTGGCATCGGCGAATTAATCGGCGGAAGTCAACGTGAAGAAAGAATGGATGTTTTA
GTAGACAGAATCAAAGAATTAGGCATGAAAGAAGAAGACTACTGGTGGTACTTAGAACTTAGAAAATACGGCGGTACAAA
ACACGCTGGATTCGGTCTAGGCTTTGAACGTTTCTTAATGTACATCACTGGTATGGGGAATATCCGTGACGTAATTCCAT
TCCCAAGAACGCCAGGTTCTGCGGAGTTTTAA

Upstream 100 bases:

>100_bases
CTTTATGACAAATTGGGTGGTACCGCGAAGTTTAACCTTTCGTCCCTTTATGGATGAAAGGTTTTTTTGTATTTAAATAT
TCGTAAAGGAGACATTCACT

Downstream 100 bases:

>100_bases
AATATGAAAAAAGCGAGAGGAAGGCCCTCTCGCTTTTTCGTATAGATTTTTATTGAAAAGCAAAAAGTAACGTGAAGGGG
TGAATAGGAATGAGTAAAAA

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MENTLVKSLYRETDKYVDQKVQVSGWIRNLRDSKVFGFIELNDGSFFKSVQIVFDTELDNFKEITKLPLSSSIRVEGKFI
ATPTAKQPFEIKAEKIEIEGLSDSDYPLQKKRHTFEYLRTIAHLRPRTNAFSATFRVRSIAAYAIHKFFQERGFVHVHTP
IITGSDTEGAGEMFRVTTHDMNNLPKGEDGQVDDSKDFFGRETNLTVSGQLPAEAYALAFRDVYTFGPTFRAENSNTTRH
AAEFWMVEPEIAFAELEDVMDLTEDMLKYAMKYVLEHAPEEMEFFNKFVDKTVLERMNNVINSDFGRITYTEAIKVLQES
GADFKYPVEWGIDLQTEHERYLSEQVFKRPVFVTDYPKDIKAFYMRMNDDGKTVAATDLLVPGIGELIGGSQREERMDVL
VDRIKELGMKEEDYWWYLELRKYGGTKHAGFGLGFERFLMYITGMGNIRDVIPFPRTPGSAEF

Sequences:

>Translated_463_residues
MENTLVKSLYRETDKYVDQKVQVSGWIRNLRDSKVFGFIELNDGSFFKSVQIVFDTELDNFKEITKLPLSSSIRVEGKFI
ATPTAKQPFEIKAEKIEIEGLSDSDYPLQKKRHTFEYLRTIAHLRPRTNAFSATFRVRSIAAYAIHKFFQERGFVHVHTP
IITGSDTEGAGEMFRVTTHDMNNLPKGEDGQVDDSKDFFGRETNLTVSGQLPAEAYALAFRDVYTFGPTFRAENSNTTRH
AAEFWMVEPEIAFAELEDVMDLTEDMLKYAMKYVLEHAPEEMEFFNKFVDKTVLERMNNVINSDFGRITYTEAIKVLQES
GADFKYPVEWGIDLQTEHERYLSEQVFKRPVFVTDYPKDIKAFYMRMNDDGKTVAATDLLVPGIGELIGGSQREERMDVL
VDRIKELGMKEEDYWWYLELRKYGGTKHAGFGLGFERFLMYITGMGNIRDVIPFPRTPGSAEF
>Mature_463_residues
MENTLVKSLYRETDKYVDQKVQVSGWIRNLRDSKVFGFIELNDGSFFKSVQIVFDTELDNFKEITKLPLSSSIRVEGKFI
ATPTAKQPFEIKAEKIEIEGLSDSDYPLQKKRHTFEYLRTIAHLRPRTNAFSATFRVRSIAAYAIHKFFQERGFVHVHTP
IITGSDTEGAGEMFRVTTHDMNNLPKGEDGQVDDSKDFFGRETNLTVSGQLPAEAYALAFRDVYTFGPTFRAENSNTTRH
AAEFWMVEPEIAFAELEDVMDLTEDMLKYAMKYVLEHAPEEMEFFNKFVDKTVLERMNNVINSDFGRITYTEAIKVLQES
GADFKYPVEWGIDLQTEHERYLSEQVFKRPVFVTDYPKDIKAFYMRMNDDGKTVAATDLLVPGIGELIGGSQREERMDVL
VDRIKELGMKEEDYWWYLELRKYGGTKHAGFGLGFERFLMYITGMGNIRDVIPFPRTPGSAEF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=448, Percent_Identity=45.9821428571429, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI4758762, Length=455, Percent_Identity=29.2307692307692, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI45439306, Length=341, Percent_Identity=28.7390029325513, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1787161, Length=451, Percent_Identity=57.4279379157428, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1790571, Length=426, Percent_Identity=26.9953051643192, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789256, Length=421, Percent_Identity=25.1781472684086, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788173, Length=109, Percent_Identity=35.7798165137615, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71984122, Length=467, Percent_Identity=29.9785867237687, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17556372, Length=425, Percent_Identity=31.7647058823529, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17551876, Length=349, Percent_Identity=28.6532951289398, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319872, Length=488, Percent_Identity=38.9344262295082, Blast_Score=320, Evalue=4e-88,
Organism=Saccharomyces cerevisiae, GI6321807, Length=463, Percent_Identity=30.0215982721382, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=346, Percent_Identity=29.7687861271676, Blast_Score=123, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922416, Length=455, Percent_Identity=36.9230769230769, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI19921528, Length=457, Percent_Identity=28.6652078774617, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17136276, Length=350, Percent_Identity=28.2857142857143, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 53415; Mature: 53415

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTLVKSLYRETDKYVDQKVQVSGWIRNLRDSKVFGFIELNDGSFFKSVQIVFDTELDN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEEEECCCHH
FKEITKLPLSSSIRVEGKFIATPTAKQPFEIKAEKIEIEGLSDSDYPLQKKRHTFEYLRT
HHHHHHCCCCCCEEECEEEEECCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
IAHLRPRTNAFSATFRVRSIAAYAIHKFFQERGFVHVHTPIITGSDTEGAGEMFRVTTHD
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCCCEEEEEECC
MNNLPKGEDGQVDDSKDFFGRETNLTVSGQLPAEAYALAFRDVYTFGPTFRAENSNTTRH
CCCCCCCCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCHHH
AAEFWMVEPEIAFAELEDVMDLTEDMLKYAMKYVLEHAPEEMEFFNKFVDKTVLERMNNV
HHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
INSDFGRITYTEAIKVLQESGADFKYPVEWGIDLQTEHERYLSEQVFKRPVFVTDYPKDI
HCCCCCCEEHHHHHHHHHHCCCCEEECCEECCCCCHHHHHHHHHHHHHCCCEEECCCHHH
KAFYMRMNDDGKTVAATDLLVPGIGELIGGSQREERMDVLVDRIKELGMKEEDYWWYLEL
EEEEEEECCCCCEEEEHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEE
RKYGGTKHAGFGLGFERFLMYITGMGNIRDVIPFPRTPGSAEF
EECCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure
MENTLVKSLYRETDKYVDQKVQVSGWIRNLRDSKVFGFIELNDGSFFKSVQIVFDTELDN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEEEECCCHH
FKEITKLPLSSSIRVEGKFIATPTAKQPFEIKAEKIEIEGLSDSDYPLQKKRHTFEYLRT
HHHHHHCCCCCCEEECEEEEECCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
IAHLRPRTNAFSATFRVRSIAAYAIHKFFQERGFVHVHTPIITGSDTEGAGEMFRVTTHD
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCCCEEEEEECC
MNNLPKGEDGQVDDSKDFFGRETNLTVSGQLPAEAYALAFRDVYTFGPTFRAENSNTTRH
CCCCCCCCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCHHH
AAEFWMVEPEIAFAELEDVMDLTEDMLKYAMKYVLEHAPEEMEFFNKFVDKTVLERMNNV
HHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
INSDFGRITYTEAIKVLQESGADFKYPVEWGIDLQTEHERYLSEQVFKRPVFVTDYPKDI
HCCCCCCEEHHHHHHHHHHCCCCEEECCEECCCCCHHHHHHHHHHHHHCCCEEECCCHHH
KAFYMRMNDDGKTVAATDLLVPGIGELIGGSQREERMDVLVDRIKELGMKEEDYWWYLEL
EEEEEEECCCCCEEEEHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEE
RKYGGTKHAGFGLGFERFLMYITGMGNIRDVIPFPRTPGSAEF
EECCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]