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The map label for this gene is folC [H]

Identifier: 152976874

GI number: 152976874

Start: 3221517

End: 3222818

Strand: Reverse

Name: folC [H]

Synonym: Bcer98_3173

Alternate gene names: 152976874

Gene position: 3222818-3221517 (Counterclockwise)

Preceding gene: 152976875

Following gene: 152976873

Centisome position: 78.85

GC content: 36.18

Gene sequence:

>1302_bases
GTGGTACATACATACGAAGAAGCAATTGGCTGGATTCATAGCCGATTAAAATTTGGGATTAAACCGGGATTAGAAAGAAT
GCGGTGGATGCTTGGAGAGCTTGACAATCCAGAGCGGCATATAAAATGTATTCATTTAGCAGGTACAAATGGTAAAGGTT
CGACTTTAACATATATGCGCTATATGCTAGAGGCAGAGACATATAAAGTAGGAACATTTACATCTCCGTATATTGAAACA
TTTAATGAACGGATTAGTGTGAATGGAAAGCCAATTTCAGATCAAGAGATTACTGACCTTGTCAATATAGTAAAGCCTGT
TGTAGAAAAGTTGGATGAGACAGAGTTTGGAGAAGCAACGGAGTTTGAAATTATTACAGTCATGGCAATGTATTATTTCG
GAAAAGTGAACATTTGCGATCTCGTTTTATTTGAAACAGGTCTTGGTGGGCGTTTCGATTCTACTAATGTTGTGTATCCC
GTTTTAACAATCATTACAAATATTGGTCATGACCATATGCATATTCTAGGAAATACATTAGCAGAAATCGCATATGAAAA
AGCGGGAATTATAAAATCAGGGGTACCGGTAATTACGGGAGTGCAAGATGGAGAAGCACTTCAGGTGATTCAAAAGGTTG
CAAATGAAAAGAATGCTAGCCTTTATGAGATAGGAAAACAATTTACAGTCGTGCACCAGCGTTCTAATGAAGAGGGAGAG
CACTTTGACTTTTCTTGTCCATTTGCTTCATTTAAAGATATACAAATTTCTATGAAAGGAGATCATCAAGTAAGAAATGC
AGCACTTGCGTTAATGGCGGTGTTATATTTGAAAACATACATGTCATTTTTAGTGGAAGAAGAAAATATTCGCATAGGTT
TAAGTGAAACGTATTGGATTGGTCGATTCGAAAGATTAAGACGTAATCCAGATGTGATTATAGATGGCGCACATAATCCA
GAAGGCATAGCAAGCCTTGTTCAAACGGCAAAAGCGCATTACAGTGACAAGAATGTGAGTATTTTATTTACGGCTCTTGG
TGATAAAGAACTAAGTCATATGGTAGGACAATTGGAAGCGGTAGCGGATGAAATGATTTTTACAACTTTTGATTTTAATC
GTGCAATCTCTGCTCAAGAATTAGCAAATTATGCAACTAAGGAAACAAAAAAAGTAGTTGAGAATTGGAAAGAGGCAATT
GATCAAAAGTTACTAGAGTTGCAAGAAAATGATGTGTTTATCATAACAGGTTCTCTTTATTTCATCTCAGAAGCTCGAAA
ATATATTGTTGAGAAAAACTAG

Upstream 100 bases:

>100_bases
GACTCCCTATCTTGGTGAGTCTCTTTTATTTGCGTATAATAGAGTTACAGTTCATTTGTGATATTGGTTTGCTCGTTGAT
TGGAAAGGGGATTGACTCGC

Downstream 100 bases:

>100_bases
GATAGCGATAGCTATCCTAGTTTTTTTTTGTGCTACAAATGAAGTCAAATACATATACATAAAGTAACTATATAGGGCAA
TTTGACGAATCTTTTTGACA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MVHTYEEAIGWIHSRLKFGIKPGLERMRWMLGELDNPERHIKCIHLAGTNGKGSTLTYMRYMLEAETYKVGTFTSPYIET
FNERISVNGKPISDQEITDLVNIVKPVVEKLDETEFGEATEFEIITVMAMYYFGKVNICDLVLFETGLGGRFDSTNVVYP
VLTIITNIGHDHMHILGNTLAEIAYEKAGIIKSGVPVITGVQDGEALQVIQKVANEKNASLYEIGKQFTVVHQRSNEEGE
HFDFSCPFASFKDIQISMKGDHQVRNAALALMAVLYLKTYMSFLVEEENIRIGLSETYWIGRFERLRRNPDVIIDGAHNP
EGIASLVQTAKAHYSDKNVSILFTALGDKELSHMVGQLEAVADEMIFTTFDFNRAISAQELANYATKETKKVVENWKEAI
DQKLLELQENDVFIITGSLYFISEARKYIVEKN

Sequences:

>Translated_433_residues
MVHTYEEAIGWIHSRLKFGIKPGLERMRWMLGELDNPERHIKCIHLAGTNGKGSTLTYMRYMLEAETYKVGTFTSPYIET
FNERISVNGKPISDQEITDLVNIVKPVVEKLDETEFGEATEFEIITVMAMYYFGKVNICDLVLFETGLGGRFDSTNVVYP
VLTIITNIGHDHMHILGNTLAEIAYEKAGIIKSGVPVITGVQDGEALQVIQKVANEKNASLYEIGKQFTVVHQRSNEEGE
HFDFSCPFASFKDIQISMKGDHQVRNAALALMAVLYLKTYMSFLVEEENIRIGLSETYWIGRFERLRRNPDVIIDGAHNP
EGIASLVQTAKAHYSDKNVSILFTALGDKELSHMVGQLEAVADEMIFTTFDFNRAISAQELANYATKETKKVVENWKEAI
DQKLLELQENDVFIITGSLYFISEARKYIVEKN
>Mature_433_residues
MVHTYEEAIGWIHSRLKFGIKPGLERMRWMLGELDNPERHIKCIHLAGTNGKGSTLTYMRYMLEAETYKVGTFTSPYIET
FNERISVNGKPISDQEITDLVNIVKPVVEKLDETEFGEATEFEIITVMAMYYFGKVNICDLVLFETGLGGRFDSTNVVYP
VLTIITNIGHDHMHILGNTLAEIAYEKAGIIKSGVPVITGVQDGEALQVIQKVANEKNASLYEIGKQFTVVHQRSNEEGE
HFDFSCPFASFKDIQISMKGDHQVRNAALALMAVLYLKTYMSFLVEEENIRIGLSETYWIGRFERLRRNPDVIIDGAHNP
EGIASLVQTAKAHYSDKNVSILFTALGDKELSHMVGQLEAVADEMIFTTFDFNRAISAQELANYATKETKKVVENWKEAI
DQKLLELQENDVFIITGSLYFISEARKYIVEKN

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=404, Percent_Identity=27.2277227722772, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI66932984, Length=404, Percent_Identity=27.2277227722772, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788654, Length=422, Percent_Identity=27.0142180094787, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17553150, Length=388, Percent_Identity=26.2886597938144, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=391, Percent_Identity=26.0869565217391, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=391, Percent_Identity=26.0869565217391, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=431, Percent_Identity=28.538283062645, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324815, Length=365, Percent_Identity=27.9452054794521, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6322718, Length=367, Percent_Identity=20.9809264305177, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641571, Length=182, Percent_Identity=39.5604395604396, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24581568, Length=187, Percent_Identity=30.4812834224599, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 49008; Mature: 49008

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHTYEEAIGWIHSRLKFGIKPGLERMRWMLGELDNPERHIKCIHLAGTNGKGSTLTYMR
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHH
YMLEAETYKVGTFTSPYIETFNERISVNGKPISDQEITDLVNIVKPVVEKLDETEFGEAT
HHHHHCEEEECCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
EFEIITVMAMYYFGKVNICDLVLFETGLGGRFDSTNVVYPVLTIITNIGHDHMHILGNTL
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
AEIAYEKAGIIKSGVPVITGVQDGEALQVIQKVANEKNASLYEIGKQFTVVHQRSNEEGE
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCCC
HFDFSCPFASFKDIQISMKGDHQVRNAALALMAVLYLKTYMSFLVEEENIRIGLSETYWI
CEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEECCCCCHHH
GRFERLRRNPDVIIDGAHNPEGIASLVQTAKAHYSDKNVSILFTALGDKELSHMVGQLEA
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
VADEMIFTTFDFNRAISAQELANYATKETKKVVENWKEAIDQKLLELQENDVFIITGSLY
HHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
FISEARKYIVEKN
HHHHHHHHHCCCC
>Mature Secondary Structure
MVHTYEEAIGWIHSRLKFGIKPGLERMRWMLGELDNPERHIKCIHLAGTNGKGSTLTYMR
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHH
YMLEAETYKVGTFTSPYIETFNERISVNGKPISDQEITDLVNIVKPVVEKLDETEFGEAT
HHHHHCEEEECCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
EFEIITVMAMYYFGKVNICDLVLFETGLGGRFDSTNVVYPVLTIITNIGHDHMHILGNTL
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
AEIAYEKAGIIKSGVPVITGVQDGEALQVIQKVANEKNASLYEIGKQFTVVHQRSNEEGE
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCCC
HFDFSCPFASFKDIQISMKGDHQVRNAALALMAVLYLKTYMSFLVEEENIRIGLSETYWI
CEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEECCCCCHHH
GRFERLRRNPDVIIDGAHNPEGIASLVQTAKAHYSDKNVSILFTALGDKELSHMVGQLEA
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
VADEMIFTTFDFNRAISAQELANYATKETKKVVENWKEAIDQKLLELQENDVFIITGSLY
HHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
FISEARKYIVEKN
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]