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The map label for this gene is pbpI [H]

Identifier: 152976792

GI number: 152976792

Start: 3131407

End: 3133161

Strand: Reverse

Name: pbpI [H]

Synonym: Bcer98_3090

Alternate gene names: 152976792

Gene position: 3133161-3131407 (Counterclockwise)

Preceding gene: 152976793

Following gene: 152976791

Centisome position: 76.66

GC content: 36.92

Gene sequence:

>1755_bases
ATGAAAATGAAGCGAAGAATTGTTATCATTTTATTATGTTTTACATGTATTATGCTCCTTTTGCTTGGTAGACTGGCGCA
AATACAACTTATATCTACTGAATCATTTACAGATAGGCAAATTAATTTAATTGAAAAGAGTGTCACCCAGCGTACACAAG
CTTTTACAGTGGATGAAGGGAGGGGGCATTTTGTTGATCGAAATGGAGAAAGAATCGGTGAAAAAAGCTATCCCGCTTTA
ATAATTTTTCCGTTCTTGCAAATAAAAAATGACATGTTACAAAAAATTGCGCATATCATTGGTGTGTCAGGGCAAGACAT
ACATTTGCAAATGAAAAATAAAAGAAAACCATTCCTATTTCAAAGAGGTGATCAGCCGTTTCAGTTAACGAAAGAACAGA
TGGAAAAGATAAATCGTGAAAATATGTTAGGGATGGTAGCGACTGAAGTAAGGTTAAAACAAACATCCGAAGCAGAACAT
TTCATTGGTGTAGTAGGAGAAAATGAGAGGGAGTTTCAAAAACGATATGGAAAGAAACATAAGTTATCTAATCAAACCCC
TGTCGGTATTTCAGGGTTACAACAGTCATTTGATGAATTTTTAATGACTGATGGTGAAGCAAAAATATTATATCAAGTGG
ATAGACGGGGGGAGCCCATTTTTGGAAAGCATGCGAAACTTACCTCTCCTGGAAATCCGTTTTATCCTGTCACGATTCAA
ACAACGCTTCATAAACGGATGCAACAAAAAGCAGAAGCGCTCCTGGAAATTTATAGGATGAAAAGGGGCGGATTAGTACT
ATTAGATGTGGAAAATAATGAAATTTTAGCTATGGTAAGTAAGCCGTCTATACAGCAAAATGATAAGAGTACATATGAAC
AGGCGTTGGAAAACCAAATGGTAACACCTCATTTTCCAGGGTCTATTTTTAAGACGGTGATAGCTGCGGCAGCAATAGAT
CAAAAATTACTTCAGCCTAACAGAATGTTTAATTGTGATACAGATTTGTATGGTGAAGATATTCCAGAAGTAATGATGGG
AAAATTAAACTTTAAAGAGAGTTTTGCTAGAAGTTGTAACCGAACGTTTGCTGAACTCGGGAATGAATTAATGCAGCTTG
ATAAAAGTGTGATGGAAACATATTTAAAAGCGCTCGGTGGAAGTGGGGACGTTGGCTGGACGGGACCGGTATTTCATGCA
TTAAAGTTTAAACAGTTGCCAGAAGAAAAAAAAGCTGTTGTTTGGGGGGAGGAAGAAAATAAAAGAAGCTATAAAGCAAT
TGCACAAACTGCAATTGGACAAAAAGATGTACGAATTTCGCCGTTAGCAGTCGCAAATATGATGGCAACGATTGCTCGGG
GCGGAGAGAAACGTGAAGTAAAGGCTGTAAAAGAAATTCGATATAAGAATGGAACGAGTTTTTTTCAATTTAAAGATCAC
AAGGTAGAAGGAAAGCGCCTTTCTTATACAACGATAACAAAGTTACAACAATTGTTACGGAGTGTAGTAACAATGAAGGA
AGGGACGGGTTCAGCTTTTCAAGGCTTACCTGTATCAGTTGCTGGAAAGTCCGGTACAGCACAAACCGGAAAGGGAAATA
AAGTAAATCGATGGTTCGCTGGTTATTTTCCTTATGATAACCCTAAATATGCTTTAGTTGTAGTGGATATTGAAACAACT
CAAAACCAAAATATAGTAACATCCATTTTCAAAGAGTTTGTAGAAGCGATTTATCAATTAGAGCATGCACAGTAA

Upstream 100 bases:

>100_bases
TGAGGAAGTTTTTAAAGCAACCAGCAAATCAAAGGTAATAGGAGGTTTTGTATAAAAAGAATAATGCCTCGTCTAAACTG
AAAAGACGAGGTGTTTTTTT

Downstream 100 bases:

>100_bases
TCTTGCAATTGTATGTAAAATGTTTTCAGTTTGTGAAAAAATGTTTGTAATTGTTCAACCTTTTTAGATTTAATGTTACA
ATAGCAAGTAGGAAAAACTG

Product: penicillin-binding protein transpeptidase

Products: NA

Alternate protein names: PBP-4B; PBP4b; Penicillin-binding protein I [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MKMKRRIVIILLCFTCIMLLLLGRLAQIQLISTESFTDRQINLIEKSVTQRTQAFTVDEGRGHFVDRNGERIGEKSYPAL
IIFPFLQIKNDMLQKIAHIIGVSGQDIHLQMKNKRKPFLFQRGDQPFQLTKEQMEKINRENMLGMVATEVRLKQTSEAEH
FIGVVGENEREFQKRYGKKHKLSNQTPVGISGLQQSFDEFLMTDGEAKILYQVDRRGEPIFGKHAKLTSPGNPFYPVTIQ
TTLHKRMQQKAEALLEIYRMKRGGLVLLDVENNEILAMVSKPSIQQNDKSTYEQALENQMVTPHFPGSIFKTVIAAAAID
QKLLQPNRMFNCDTDLYGEDIPEVMMGKLNFKESFARSCNRTFAELGNELMQLDKSVMETYLKALGGSGDVGWTGPVFHA
LKFKQLPEEKKAVVWGEEENKRSYKAIAQTAIGQKDVRISPLAVANMMATIARGGEKREVKAVKEIRYKNGTSFFQFKDH
KVEGKRLSYTTITKLQQLLRSVVTMKEGTGSAFQGLPVSVAGKSGTAQTGKGNKVNRWFAGYFPYDNPKYALVVVDIETT
QNQNIVTSIFKEFVEAIYQLEHAQ

Sequences:

>Translated_584_residues
MKMKRRIVIILLCFTCIMLLLLGRLAQIQLISTESFTDRQINLIEKSVTQRTQAFTVDEGRGHFVDRNGERIGEKSYPAL
IIFPFLQIKNDMLQKIAHIIGVSGQDIHLQMKNKRKPFLFQRGDQPFQLTKEQMEKINRENMLGMVATEVRLKQTSEAEH
FIGVVGENEREFQKRYGKKHKLSNQTPVGISGLQQSFDEFLMTDGEAKILYQVDRRGEPIFGKHAKLTSPGNPFYPVTIQ
TTLHKRMQQKAEALLEIYRMKRGGLVLLDVENNEILAMVSKPSIQQNDKSTYEQALENQMVTPHFPGSIFKTVIAAAAID
QKLLQPNRMFNCDTDLYGEDIPEVMMGKLNFKESFARSCNRTFAELGNELMQLDKSVMETYLKALGGSGDVGWTGPVFHA
LKFKQLPEEKKAVVWGEEENKRSYKAIAQTAIGQKDVRISPLAVANMMATIARGGEKREVKAVKEIRYKNGTSFFQFKDH
KVEGKRLSYTTITKLQQLLRSVVTMKEGTGSAFQGLPVSVAGKSGTAQTGKGNKVNRWFAGYFPYDNPKYALVVVDIETT
QNQNIVTSIFKEFVEAIYQLEHAQ
>Mature_584_residues
MKMKRRIVIILLCFTCIMLLLLGRLAQIQLISTESFTDRQINLIEKSVTQRTQAFTVDEGRGHFVDRNGERIGEKSYPAL
IIFPFLQIKNDMLQKIAHIIGVSGQDIHLQMKNKRKPFLFQRGDQPFQLTKEQMEKINRENMLGMVATEVRLKQTSEAEH
FIGVVGENEREFQKRYGKKHKLSNQTPVGISGLQQSFDEFLMTDGEAKILYQVDRRGEPIFGKHAKLTSPGNPFYPVTIQ
TTLHKRMQQKAEALLEIYRMKRGGLVLLDVENNEILAMVSKPSIQQNDKSTYEQALENQMVTPHFPGSIFKTVIAAAAID
QKLLQPNRMFNCDTDLYGEDIPEVMMGKLNFKESFARSCNRTFAELGNELMQLDKSVMETYLKALGGSGDVGWTGPVFHA
LKFKQLPEEKKAVVWGEEENKRSYKAIAQTAIGQKDVRISPLAVANMMATIARGGEKREVKAVKEIRYKNGTSFFQFKDH
KVEGKRLSYTTITKLQQLLRSVVTMKEGTGSAFQGLPVSVAGKSGTAQTGKGNKVNRWFAGYFPYDNPKYALVVVDIETT
QNQNIVTSIFKEFVEAIYQLEHAQ

Specific function: Penicillin-binding protein with an unknown catalytic activity. Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active site of these p

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=569, Percent_Identity=23.1985940246046, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 66245; Mature: 66245

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKRRIVIILLCFTCIMLLLLGRLAQIQLISTESFTDRQINLIEKSVTQRTQAFTVDEG
CCCCHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEECC
RGHFVDRNGERIGEKSYPALIIFPFLQIKNDMLQKIAHIIGVSGQDIHLQMKNKRKPFLF
CCCEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEE
QRGDQPFQLTKEQMEKINRENMLGMVATEVRLKQTSEAEHFIGVVGENEREFQKRYGKKH
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCHH
KLSNQTPVGISGLQQSFDEFLMTDGEAKILYQVDRRGEPIFGKHAKLTSPGNPFYPVTIQ
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEECCCCCCEEEEEEH
TTLHKRMQQKAEALLEIYRMKRGGLVLLDVENNEILAMVSKPSIQQNDKSTYEQALENQM
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCC
VTPHFPGSIFKTVIAAAAIDQKLLQPNRMFNCDTDLYGEDIPEVMMGKLNFKESFARSCN
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
RTFAELGNELMQLDKSVMETYLKALGGSGDVGWTGPVFHALKFKQLPEEKKAVVWGEEEN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCC
KRSYKAIAQTAIGQKDVRISPLAVANMMATIARGGEKREVKAVKEIRYKNGTSFFQFKDH
HHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECC
KVEGKRLSYTTITKLQQLLRSVVTMKEGTGSAFQGLPVSVAGKSGTAQTGKGNKVNRWFA
CCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEE
GYFPYDNPKYALVVVDIETTQNQNIVTSIFKEFVEAIYQLEHAQ
EECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKMKRRIVIILLCFTCIMLLLLGRLAQIQLISTESFTDRQINLIEKSVTQRTQAFTVDEG
CCCCHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEECC
RGHFVDRNGERIGEKSYPALIIFPFLQIKNDMLQKIAHIIGVSGQDIHLQMKNKRKPFLF
CCCEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEE
QRGDQPFQLTKEQMEKINRENMLGMVATEVRLKQTSEAEHFIGVVGENEREFQKRYGKKH
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCHH
KLSNQTPVGISGLQQSFDEFLMTDGEAKILYQVDRRGEPIFGKHAKLTSPGNPFYPVTIQ
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEECCCCCCEEEEEEH
TTLHKRMQQKAEALLEIYRMKRGGLVLLDVENNEILAMVSKPSIQQNDKSTYEQALENQM
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCC
VTPHFPGSIFKTVIAAAAIDQKLLQPNRMFNCDTDLYGEDIPEVMMGKLNFKESFARSCN
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
RTFAELGNELMQLDKSVMETYLKALGGSGDVGWTGPVFHALKFKQLPEEKKAVVWGEEEN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCC
KRSYKAIAQTAIGQKDVRISPLAVANMMATIARGGEKREVKAVKEIRYKNGTSFFQFKDH
HHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECC
KVEGKRLSYTTITKLQQLLRSVVTMKEGTGSAFQGLPVSVAGKSGTAQTGKGNKVNRWFA
CCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEE
GYFPYDNPKYALVVVDIETTQNQNIVTSIFKEFVEAIYQLEHAQ
EECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]