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The map label for this gene is phoB [H]

Identifier: 152976773

GI number: 152976773

Start: 3114001

End: 3115386

Strand: Reverse

Name: phoB [H]

Synonym: Bcer98_3069

Alternate gene names: 152976773

Gene position: 3115386-3114001 (Counterclockwise)

Preceding gene: 152976776

Following gene: 152976771

Centisome position: 76.23

GC content: 37.81

Gene sequence:

>1386_bases
GTGAAGAAAATGATAAAGAAAGCATGGCCATTTGCTGTTGTTGCATCGTTAGCAATTACTTCAGTTGCAACATGGAACTT
TACACATTCGGAAACAATTAAAGCAGATAGCAAAGAGAATAACGCGAAAATCAAGAACGTTATTGTATTAATTGGGGATG
GGATGGGCCCTTCTTATATGACAGCTCATCGTTATATGAAGGACGATCCAAAAACATTTGGAATGGAGCCGACAGAATTT
GATAAACATCTTGTAGGGACGCAAAAAACATATCCAGAAGATGAGCATGAAAATATTACGGACTCTGCATCGGCTGCAAC
AGCAATGTCAGCTGGTATCAAAACATATAATGCAGCAATTTCAGTTGATAATGATAAAGCGGAAGTCAAAACCGTGCTTG
AGCAGGCAAAAGAACAAGGGAAAGCAACAGGGTTAGTTGCGACTTCTGAAATTACGCATGCAACGCCAGCTGCGTTTGGT
GCTCATGATACGAACCGTAAAAATATGGATGGTATTGCAGATGATTACTTTGATGAAAAAATTAATGGAAAACATAAAAT
AGATGTTATGCTTGGTGGCGGCATGAAAAACTTTGTGAGAAAGGATCGTAACCTTACAGAGGAATTTAAAAAATCTGGTT
ATAGTTATGTAACAGATCGAGAGCAATTGTTAAAGGATAAAAACGATCAAATTCTTGGTTTATTTGCACCAGGTGGTTTA
GATAAAATGATTGATCGCAATGATAAAACACCTTCATTAGAAGAAATGACAAATGCAGCAATTGATCGCTTAAAGAAAAA
TGATAAAGGTTTCTTCTTGATGGTAGAAGGGAGCCAAATCGATTGGGCTGGTCACGATAATGATGTTGTTGCAGCGATGA
GCGAAATGGAAGACTTTGAAAAAGCATTTAAAGCAGCAATTGAATTTGCGAAAAAGGATAAGCATACGTTAGTTGTAGCA
ACGGCTGATCATTCTACAGGAGGCTTCTCTTTAGGCGCAAATGGTGAATATAACTTTAAAGTAGATCCTCTGAAAGCTGC
TAAACGTACGCCAGATTTCATGGCAAATGAAATTGCAAAAGGTGCCAATGTAGAAGAGACATTGAAAAAGTATATTGATT
TAGAACTAACGCCAGAAGAAATTCAAGCAGTAAATGAGATTGCTCCGTCAAAAGATGTGACGAAGATTGATAACGCAATT
GAAAATATCTTCAATAAACGATCTGTTACGGGATGGACAACAGGCGGACATACCGGCGAGGATGTAAATGTATATGCATT
TGGTCCAGGTAAATACTTATTCTCAGGTGTTCAAGAAAATACAAACTTAGCAAAACGTGTATTTGATATTGTGGGTGGTG
GTGACCCGAATAAAGGTAGACGCTAA

Upstream 100 bases:

>100_bases
ACATAACTTAACAAAATGTTTACGTGAGCCTAATCTTTTCTTAACAGTTTTTCAGTAGATTGGGTAGTGGGAACTGAATA
ATCTGTTGGGGGGACAAAAA

Downstream 100 bases:

>100_bases
TTGTGAAAAAAAGCGAGGCAATTTGCCTCGCTTTTTTATCCCGCTGTTTTAGGGAAGCCTGATTGGTGAGGGCTCATAAT
CAGTGGGGGATGAAGAAAAC

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKMIKKAWPFAVVASLAITSVATWNFTHSETIKADSKENNAKIKNVIVLIGDGMGPSYMTAHRYMKDDPKTFGMEPTEF
DKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAISVDNDKAEVKTVLEQAKEQGKATGLVATSEITHATPAAFG
AHDTNRKNMDGIADDYFDEKINGKHKIDVMLGGGMKNFVRKDRNLTEEFKKSGYSYVTDREQLLKDKNDQILGLFAPGGL
DKMIDRNDKTPSLEEMTNAAIDRLKKNDKGFFLMVEGSQIDWAGHDNDVVAAMSEMEDFEKAFKAAIEFAKKDKHTLVVA
TADHSTGGFSLGANGEYNFKVDPLKAAKRTPDFMANEIAKGANVEETLKKYIDLELTPEEIQAVNEIAPSKDVTKIDNAI
ENIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR

Sequences:

>Translated_461_residues
MKKMIKKAWPFAVVASLAITSVATWNFTHSETIKADSKENNAKIKNVIVLIGDGMGPSYMTAHRYMKDDPKTFGMEPTEF
DKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAISVDNDKAEVKTVLEQAKEQGKATGLVATSEITHATPAAFG
AHDTNRKNMDGIADDYFDEKINGKHKIDVMLGGGMKNFVRKDRNLTEEFKKSGYSYVTDREQLLKDKNDQILGLFAPGGL
DKMIDRNDKTPSLEEMTNAAIDRLKKNDKGFFLMVEGSQIDWAGHDNDVVAAMSEMEDFEKAFKAAIEFAKKDKHTLVVA
TADHSTGGFSLGANGEYNFKVDPLKAAKRTPDFMANEIAKGANVEETLKKYIDLELTPEEIQAVNEIAPSKDVTKIDNAI
ENIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR
>Mature_461_residues
MKKMIKKAWPFAVVASLAITSVATWNFTHSETIKADSKENNAKIKNVIVLIGDGMGPSYMTAHRYMKDDPKTFGMEPTEF
DKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAISVDNDKAEVKTVLEQAKEQGKATGLVATSEITHATPAAFG
AHDTNRKNMDGIADDYFDEKINGKHKIDVMLGGGMKNFVRKDRNLTEEFKKSGYSYVTDREQLLKDKNDQILGLFAPGGL
DKMIDRNDKTPSLEEMTNAAIDRLKKNDKGFFLMVEGSQIDWAGHDNDVVAAMSEMEDFEKAFKAAIEFAKKDKHTLVVA
TADHSTGGFSLGANGEYNFKVDPLKAAKRTPDFMANEIAKGANVEETLKKYIDLELTPEEIQAVNEIAPSKDVTKIDNAI
ENIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI94721246, Length=476, Percent_Identity=31.7226890756302, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI157266296, Length=480, Percent_Identity=31.4583333333333, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI157266292, Length=478, Percent_Identity=30.1255230125523, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI116734717, Length=473, Percent_Identity=30.0211416490486, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI294660770, Length=446, Percent_Identity=29.5964125560538, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI294660772, Length=415, Percent_Identity=28.1927710843373, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI48994877, Length=335, Percent_Identity=37.6119402985075, Blast_Score=171, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6320689, Length=448, Percent_Identity=34.375, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21355981, Length=459, Percent_Identity=31.1546840958606, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI85725200, Length=474, Percent_Identity=29.746835443038, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI85815833, Length=474, Percent_Identity=29.746835443038, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21355151, Length=481, Percent_Identity=29.1060291060291, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI18859923, Length=430, Percent_Identity=29.0697674418605, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24651554, Length=478, Percent_Identity=31.7991631799163, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921912, Length=456, Percent_Identity=31.140350877193, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24585213, Length=480, Percent_Identity=29.375, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI21358067, Length=470, Percent_Identity=29.3617021276596, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355149, Length=470, Percent_Identity=29.3617021276596, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24657835, Length=465, Percent_Identity=29.247311827957, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24651556, Length=418, Percent_Identity=31.5789473684211, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24657827, Length=456, Percent_Identity=30.2631578947368, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24657842, Length=457, Percent_Identity=27.7899343544858, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 50690; Mature: 50690

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMIKKAWPFAVVASLAITSVATWNFTHSETIKADSKENNAKIKNVIVLIGDGMGPSYM
CHHHHHHHCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHH
TAHRYMKDDPKTFGMEPTEFDKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAI
HHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEE
SVDNDKAEVKTVLEQAKEQGKATGLVATSEITHATPAAFGAHDTNRKNMDGIADDYFDEK
EECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
INGKHKIDVMLGGGMKNFVRKDRNLTEEFKKSGYSYVTDREQLLKDKNDQILGLFAPGGL
CCCCEEEEEEECCCHHHHHHHHCCHHHHHHHCCCCEECCHHHHHCCCCCCEEEEECCCCH
DKMIDRNDKTPSLEEMTNAAIDRLKKNDKGFFLMVEGSQIDWAGHDNDVVAAMSEMEDFE
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHH
KAFKAAIEFAKKDKHTLVVATADHSTGGFSLGANGEYNFKVDPLKAAKRTPDFMANEIAK
HHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHHC
GANVEETLKKYIDLELTPEEIQAVNEIAPSKDVTKIDNAIENIFNKRSVTGWTTGGHTGE
CCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR
CEEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKMIKKAWPFAVVASLAITSVATWNFTHSETIKADSKENNAKIKNVIVLIGDGMGPSYM
CHHHHHHHCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHH
TAHRYMKDDPKTFGMEPTEFDKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAI
HHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEE
SVDNDKAEVKTVLEQAKEQGKATGLVATSEITHATPAAFGAHDTNRKNMDGIADDYFDEK
EECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
INGKHKIDVMLGGGMKNFVRKDRNLTEEFKKSGYSYVTDREQLLKDKNDQILGLFAPGGL
CCCCEEEEEEECCCHHHHHHHHCCHHHHHHHCCCCEECCHHHHHCCCCCCEEEEECCCCH
DKMIDRNDKTPSLEEMTNAAIDRLKKNDKGFFLMVEGSQIDWAGHDNDVVAAMSEMEDFE
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHH
KAFKAAIEFAKKDKHTLVVATADHSTGGFSLGANGEYNFKVDPLKAAKRTPDFMANEIAK
HHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHHC
GANVEETLKKYIDLELTPEEIQAVNEIAPSKDVTKIDNAIENIFNKRSVTGWTTGGHTGE
CCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR
CEEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]