Click here to switch to the map view.

The map label for this gene is yfiJ [H]

Identifier: 152974057

GI number: 152974057

Start: 224480

End: 225673

Strand: Direct

Name: yfiJ [H]

Synonym: Bcer98_0213

Alternate gene names: 152974057

Gene position: 224480-225673 (Clockwise)

Preceding gene: 152974056

Following gene: 152974058

Centisome position: 5.49

GC content: 34.34

Gene sequence:

>1194_bases
ATGTTAAAGTATGCACGTATCATTACGATTATTATGATCTGTTTAGTATATATCAACAATGTTTCACGTGGAACGATAGG
TTTGCAAGTATTTATTGGTATGGCATGTTTCATTTATATTTTGAATCATGTATTGCTCTTTCAGCCACAAAAAGGAAAAA
AACGTGATTTTTATATATTTCTAACCAATGGAATTGTTACAGCAATGCTTGGATTTTTATTTCCTGAAACATCTTTATAC
TTGATTATATTTGGGATTGAAGCAGTAGGAATATTTATTCACACATGGCGGAAAGAAGTTGTATATTTTTTTGTGAGTTT
TTTCTTTATATGTTGGTTTTCTATTTTGTTATATACATATCAATATACAGGGAAGGTAGAATTGGATACAAACGCCATTA
ACTTTATGTTTGTACTCTTTAGTGCTTTAGTAGGGAGTTTAATAAGAAAACTGACCGTAGCACATCAAACGGTTGATGAA
CAGTATGAAAAATTAATATCATCTCATACGGCTTTAAAAGAAGCACATGAACAACTACGGCTTTATGCTAAAGAAGTAGA
AGAATTAACTGCTGTTCGGGAACGAAACGATATTGCTAGAGAAATTCATGATACAGTAGGTCATAATATGACAGCTTTAC
TTGTACAACTGCAACTTGCCGAAGCTTTATGGAAACAAAAGTCAAATGTGACAGAAGATGTTTTGCGTACATGCCATGAA
TTAGCTAAGAAATCTCTTCAAGAGGTAAGAGCTTCGGTACGTGCACTAAAGGAAGAAAAGAATTTAGGGAATATAGTAGA
GAGTATGCGAGAAATGTTAAGGGAGTTTTCAAAAGCGGCGACAGTGCAAGTAACCTTTCAATTAAAAGGAGATCCGATTG
TTATTCCGTTATCATTACAGCCAACTCTACTACGTATTATGCAAGAATCTTTAACAAATGCGAAACGTCACGGAAAAGCT
AGTGTATGTGAAGTTGAATTATTTTGTGACAGTGAAACAGTAATGTTATCGATTTCTGATAATGGCATCGGCATGAATGA
AGTGAGCCCTGGTTTTGGATTAATGAACATGAAAGAACGCATAGAGGAACATGGAGGAGTAATTCGGTTTGAAAGTGAGA
TAGAGAAGGGATTTCGGCTAAAAGCTGAGTTCCCGTTAAAAGAAAAAACATGGGTAATAGGAGGAACGAAATGA

Upstream 100 bases:

>100_bases
TAAGAGAGGTGACTTGTTTTTCATCTATCTGTTTGTAATAAAATGGTATAATTTTTTTGTTATAGGTGAAATCCACATTT
TAAAATGCGAGGAGAATTGT

Downstream 100 bases:

>100_bases
TTCGTATTATGATTGTGGACGATCAGCCATTGATTCGTGACGGTTTAGCAATGTTATTAAACTTGCGCCCAGAACTTGAG
GTGGTAGGGACAGCAAGTGA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MLKYARIITIIMICLVYINNVSRGTIGLQVFIGMACFIYILNHVLLFQPQKGKKRDFYIFLTNGIVTAMLGFLFPETSLY
LIIFGIEAVGIFIHTWRKEVVYFFVSFFFICWFSILLYTYQYTGKVELDTNAINFMFVLFSALVGSLIRKLTVAHQTVDE
QYEKLISSHTALKEAHEQLRLYAKEVEELTAVRERNDIAREIHDTVGHNMTALLVQLQLAEALWKQKSNVTEDVLRTCHE
LAKKSLQEVRASVRALKEEKNLGNIVESMREMLREFSKAATVQVTFQLKGDPIVIPLSLQPTLLRIMQESLTNAKRHGKA
SVCEVELFCDSETVMLSISDNGIGMNEVSPGFGLMNMKERIEEHGGVIRFESEIEKGFRLKAEFPLKEKTWVIGGTK

Sequences:

>Translated_397_residues
MLKYARIITIIMICLVYINNVSRGTIGLQVFIGMACFIYILNHVLLFQPQKGKKRDFYIFLTNGIVTAMLGFLFPETSLY
LIIFGIEAVGIFIHTWRKEVVYFFVSFFFICWFSILLYTYQYTGKVELDTNAINFMFVLFSALVGSLIRKLTVAHQTVDE
QYEKLISSHTALKEAHEQLRLYAKEVEELTAVRERNDIAREIHDTVGHNMTALLVQLQLAEALWKQKSNVTEDVLRTCHE
LAKKSLQEVRASVRALKEEKNLGNIVESMREMLREFSKAATVQVTFQLKGDPIVIPLSLQPTLLRIMQESLTNAKRHGKA
SVCEVELFCDSETVMLSISDNGIGMNEVSPGFGLMNMKERIEEHGGVIRFESEIEKGFRLKAEFPLKEKTWVIGGTK
>Mature_397_residues
MLKYARIITIIMICLVYINNVSRGTIGLQVFIGMACFIYILNHVLLFQPQKGKKRDFYIFLTNGIVTAMLGFLFPETSLY
LIIFGIEAVGIFIHTWRKEVVYFFVSFFFICWFSILLYTYQYTGKVELDTNAINFMFVLFSALVGSLIRKLTVAHQTVDE
QYEKLISSHTALKEAHEQLRLYAKEVEELTAVRERNDIAREIHDTVGHNMTALLVQLQLAEALWKQKSNVTEDVLRTCHE
LAKKSLQEVRASVRALKEEKNLGNIVESMREMLREFSKAATVQVTFQLKGDPIVIPLSLQPTLLRIMQESLTNAKRHGKA
SVCEVELFCDSETVMLSISDNGIGMNEVSPGFGLMNMKERIEEHGGVIRFESEIEKGFRLKAEFPLKEKTWVIGGTK

Specific function: Member of the two-component regulatory system yfiJ/yfiK. Probably activates yfiK by phosphorylation [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=195, Percent_Identity=25.6410256410256, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082311, Length=195, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011712 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45416; Mature: 45416

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKYARIITIIMICLVYINNVSRGTIGLQVFIGMACFIYILNHVLLFQPQKGKKRDFYIF
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEE
LTNGIVTAMLGFLFPETSLYLIIFGIEAVGIFIHTWRKEVVYFFVSFFFICWFSILLYTY
EECCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYTGKVELDTNAINFMFVLFSALVGSLIRKLTVAHQTVDEQYEKLISSHTALKEAHEQLR
HCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYAKEVEELTAVRERNDIAREIHDTVGHNMTALLVQLQLAEALWKQKSNVTEDVLRTCHE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LAKKSLQEVRASVRALKEEKNLGNIVESMREMLREFSKAATVQVTFQLKGDPIVIPLSLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCEEEEEECCC
PTLLRIMQESLTNAKRHGKASVCEVELFCDSETVMLSISDNGIGMNEVSPGFGLMNMKER
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHH
IEEHGGVIRFESEIEKGFRLKAEFPLKEKTWVIGGTK
HHHHCCEEEEHHHHHCCCEEEECCCCCCCEEEECCCC
>Mature Secondary Structure
MLKYARIITIIMICLVYINNVSRGTIGLQVFIGMACFIYILNHVLLFQPQKGKKRDFYIF
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEE
LTNGIVTAMLGFLFPETSLYLIIFGIEAVGIFIHTWRKEVVYFFVSFFFICWFSILLYTY
EECCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYTGKVELDTNAINFMFVLFSALVGSLIRKLTVAHQTVDEQYEKLISSHTALKEAHEQLR
HCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYAKEVEELTAVRERNDIAREIHDTVGHNMTALLVQLQLAEALWKQKSNVTEDVLRTCHE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LAKKSLQEVRASVRALKEEKNLGNIVESMREMLREFSKAATVQVTFQLKGDPIVIPLSLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCEEEEEECCC
PTLLRIMQESLTNAKRHGKASVCEVELFCDSETVMLSISDNGIGMNEVSPGFGLMNMKER
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHH
IEEHGGVIRFESEIEKGFRLKAEFPLKEKTWVIGGTK
HHHHCCEEEEHHHHHCCCEEEECCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]