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The map label for this gene is cwlD [H]

Identifier: 152973992

GI number: 152973992

Start: 146975

End: 147688

Strand: Direct

Name: cwlD [H]

Synonym: Bcer98_0140

Alternate gene names: 152973992

Gene position: 146975-147688 (Clockwise)

Preceding gene: 152973991

Following gene: 152973993

Centisome position: 3.6

GC content: 38.38

Gene sequence:

>714_bases
ATGAAGAGAATTCGAATAATCTCTTTAGTGCTAGCTGCGATTATATTGTTTTTCTTGATAAAACAGGAATTGCAAATTAC
AAAGTCGTGGCGGGCCTGGAATTTACCCCTATCAGGAAAGGTAATTGTGCTTGATGCTGGACATGGTGGGCCAGATGGGG
GAGCGGTTGGTGGGAAAGATATTGTAGAAAAAAATATTACACTTGAAATTACAAAAATGCTGCAAGACTACTTACAGGAA
CAAGGGGCTTTGGTGATTTTAACGAGAGAAGGAGACTATGATTTAGCCGATAAAGATACAAAATCGTATAGTAGGCGTAA
GGCAGAAGATTTAAAAAAACGTGTAGCAATGATTAATAAGCCAGATGTGGATTTTTTTGTGAGTATTCATTTGAACGCTT
TGCCTAGTAATAGCTCAAAGGGAGCGCAAACATTTTATCACCGCTCTTTGCTTGAAAATGAACGAGCGGCAAAGTTTATA
CAAGCTGAACTGCGAACGAGCTTAGAGAATACGAACCGTTCCGCTAAAACAATTTCGCATGTCTATTTACTTAAGTATGC
TAAGACTCCAGGTACATTGGTTGAGGCTGGTTTCTTATCAAATGTAAATGAAAGGTATATGTTAAATTCGGAAAAATATC
AGCAAAAAGTAGCAGCTGCTATTTATCGCGGGATTTTGCGCTATTTCACAGAAAAAGGAAACCCTCCTGAATGA

Upstream 100 bases:

>100_bases
AGGTGAAAGAAGATATCGTTTAAAGTTGTATTAAGAGAAGCATAAACTTTCCTCTTGTCCCATATAAGTAATTAGAGGGA
CTAGCTGGAGGAGGAAAGGT

Downstream 100 bases:

>100_bases
GGAGGGTTTTTGCGTTTCTCTCATTAACGAAGTTTTCGGAAAATATGTTATACTGGAAATGTAAACGCATTTATTTGAAG
GAAAAGAGGATAAACCCAAT

Product: N-acetylmuramoyl-L-alanine amidase CwlD

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKRIRIISLVLAAIILFFLIKQELQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIVEKNITLEITKMLQDYLQE
QGALVILTREGDYDLADKDTKSYSRRKAEDLKKRVAMINKPDVDFFVSIHLNALPSNSSKGAQTFYHRSLLENERAAKFI
QAELRTSLENTNRSAKTISHVYLLKYAKTPGTLVEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE

Sequences:

>Translated_237_residues
MKRIRIISLVLAAIILFFLIKQELQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIVEKNITLEITKMLQDYLQE
QGALVILTREGDYDLADKDTKSYSRRKAEDLKKRVAMINKPDVDFFVSIHLNALPSNSSKGAQTFYHRSLLENERAAKFI
QAELRTSLENTNRSAKTISHVYLLKYAKTPGTLVEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE
>Mature_237_residues
MKRIRIISLVLAAIILFFLIKQELQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIVEKNITLEITKMLQDYLQE
QGALVILTREGDYDLADKDTKSYSRRKAEDLKKRVAMINKPDVDFFVSIHLNALPSNSSKGAQTFYHRSLLENERAAKFI
QAELRTSLENTNRSAKTISHVYLLKYAKTPGTLVEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE

Specific function: Cell-Wall Hydrolase Probably Involved In Cell-Wall Hydrolysis, Septation Or Recycling. [C]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=238, Percent_Identity=27.7310924369748, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082163, Length=235, Percent_Identity=28.5106382978723, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=27.3504273504274, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26734; Mature: 26734

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIRIISLVLAAIILFFLIKQELQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEECCCCCCCCCCCCCHH
IVEKNITLEITKMLQDYLQEQGALVILTREGDYDLADKDTKSYSRRKAEDLKKRVAMINK
HHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PDVDFFVSIHLNALPSNSSKGAQTFYHRSLLENERAAKFIQAELRTSLENTNRSAKTISH
CCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VYLLKYAKTPGTLVEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE
HHHHHCCCCCCHHHHHHHHHCCCHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRIRIISLVLAAIILFFLIKQELQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEECCCCCCCCCCCCCHH
IVEKNITLEITKMLQDYLQEQGALVILTREGDYDLADKDTKSYSRRKAEDLKKRVAMINK
HHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PDVDFFVSIHLNALPSNSSKGAQTFYHRSLLENERAAKFIQAELRTSLENTNRSAKTISH
CCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VYLLKYAKTPGTLVEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE
HHHHHCCCCCCHHHHHHHHHCCCHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]