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The map label for this gene is thiK [H]

Identifier: 152969657

GI number: 152969657

Start: 1251857

End: 1252684

Strand: Direct

Name: thiK [H]

Synonym: KPN_01103

Alternate gene names: 152969657

Gene position: 1251857-1252684 (Clockwise)

Preceding gene: 152969656

Following gene: 152969658

Centisome position: 23.55

GC content: 63.04

Gene sequence:

>828_bases
GTGCTGTTCAGCAACAATAAGCTAACGCGCGACGAGCTCTTGTCGCGCTTCTTTCCCCATTATCATCCTGTCGCCTCGCT
GAGCCAGAGTGGCCTCAGCGGCGGCAGTGTGATTATCAGCGACGGCGACCGGCGGCACGTGCTGCGTCAGCCGCACGATC
CGTCTGCCGCCACTTACCATTTTCGCCGTCAGTATCACGCACTCCGCCGACTGCCTGCCAGGCTGGCGCCAACCCCGCTG
TTTTACTCACCCTGCTGGTTGGTGGTGGAGTATTGCGCAGGGGAGGTGAAAAGCGAGTTACCGGCCTCCCCGCAGCTGTC
AGGCTTATTGTATGATTTGCATCAGCAACCGCGGTTTGGCTGGCGGGTTAGCCTCACACCACTGCTGGCCCAGTACTGGC
AGAGGTGCGATCCGGCGCGGCGCAAGCCGCGTTGGCTGCGCTGGCATCAGCGGCTGCGCCGCCAGGGCGAGCCCCGTCCC
CTGCGGCTGGCGCCGCTGCACATGGACGTCCATGCCGGCAATATTATTCATAATGAGTCCGGCCTGAGGCTGATCGACTG
GGAATATGCCGGCGATGGCGATATCGCGCTGGAGCTGGCCGCGGTGTGGATCACGCCTGGCGAGCGCCGTCGGCTGGTAG
AAGCGTATGCCCGGCGGGCGGCCATCGACGCGCAGCAGCTATGGCGGCAGGTGGCGCGCTGGCGCCCGTGGGTACTGCTG
CTGATGGCGGGCTGGTATGAAATGCGCTGGCGGCAGAGCGGGGACCGGCAATTTATCACGCTGGCAGATGAAATATGGTG
TCAGTTAGACAACGAACGTAAAGGATAA

Upstream 100 bases:

>100_bases
TATGTGCTTTACTCCAATGCCACCGGCAACGTCAACGCGCCTGAGCTGAAAATGCAGCTCATGCTGGTGCAAACCGGTGA
GATAATCTGGTCAGGTAAAG

Downstream 100 bases:

>100_bases
GAGAGGTCAGTGTGGGCCCAGTGATGTTGGATGTCGAAGGGTATGAGCTGGATGCGGAAGAGCGAGAAATTCTGGCGCAT
CCGCTGGTGGGAGGGTTGAT

Product: thiamine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLFSNNKLTRDELLSRFFPHYHPVASLSQSGLSGGSVIISDGDRRHVLRQPHDPSAATYHFRRQYHALRRLPARLAPTPL
FYSPCWLVVEYCAGEVKSELPASPQLSGLLYDLHQQPRFGWRVSLTPLLAQYWQRCDPARRKPRWLRWHQRLRRQGEPRP
LRLAPLHMDVHAGNIIHNESGLRLIDWEYAGDGDIALELAAVWITPGERRRLVEAYARRAAIDAQQLWRQVARWRPWVLL
LMAGWYEMRWRQSGDRQFITLADEIWCQLDNERKG

Sequences:

>Translated_275_residues
MLFSNNKLTRDELLSRFFPHYHPVASLSQSGLSGGSVIISDGDRRHVLRQPHDPSAATYHFRRQYHALRRLPARLAPTPL
FYSPCWLVVEYCAGEVKSELPASPQLSGLLYDLHQQPRFGWRVSLTPLLAQYWQRCDPARRKPRWLRWHQRLRRQGEPRP
LRLAPLHMDVHAGNIIHNESGLRLIDWEYAGDGDIALELAAVWITPGERRRLVEAYARRAAIDAQQLWRQVARWRPWVLL
LMAGWYEMRWRQSGDRQFITLADEIWCQLDNERKG
>Mature_275_residues
MLFSNNKLTRDELLSRFFPHYHPVASLSQSGLSGGSVIISDGDRRHVLRQPHDPSAATYHFRRQYHALRRLPARLAPTPL
FYSPCWLVVEYCAGEVKSELPASPQLSGLLYDLHQQPRFGWRVSLTPLLAQYWQRCDPARRKPRWLRWHQRLRRQGEPRP
LRLAPLHMDVHAGNIIHNESGLRLIDWEYAGDGDIALELAAVWITPGERRRLVEAYARRAAIDAQQLWRQVARWRPWVLL
LMAGWYEMRWRQSGDRQFITLADEIWCQLDNERKG

Specific function: Catalyzes the phosphorylation of thiamine to thiamine phosphate [H]

COG id: COG0510

COG function: function code M; Predicted choline kinase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787349, Length=267, Percent_Identity=64.0449438202247, Blast_Score=338, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR014093 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.89 [H]

Molecular weight: Translated: 32295; Mature: 32295

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSNNKLTRDELLSRFFPHYHPVASLSQSGLSGGSVIISDGDRRHVLRQPHDPSAATYH
CCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCCHHHCCCCCCCCHHHHH
FRRQYHALRRLPARLAPTPLFYSPCWLVVEYCAGEVKSELPASPQLSGLLYDLHQQPRFG
HHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
WRVSLTPLLAQYWQRCDPARRKPRWLRWHQRLRRQGEPRPLRLAPLHMDVHAGNIIHNES
EEEEHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCEEECCC
GLRLIDWEYAGDGDIALELAAVWITPGERRRLVEAYARRAAIDAQQLWRQVARWRPWVLL
CCEEEEEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHH
LMAGWYEMRWRQSGDRQFITLADEIWCQLDNERKG
HHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCC
>Mature Secondary Structure
MLFSNNKLTRDELLSRFFPHYHPVASLSQSGLSGGSVIISDGDRRHVLRQPHDPSAATYH
CCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCCHHHCCCCCCCCHHHHH
FRRQYHALRRLPARLAPTPLFYSPCWLVVEYCAGEVKSELPASPQLSGLLYDLHQQPRFG
HHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
WRVSLTPLLAQYWQRCDPARRKPRWLRWHQRLRRQGEPRPLRLAPLHMDVHAGNIIHNES
EEEEHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCEEECCC
GLRLIDWEYAGDGDIALELAAVWITPGERRRLVEAYARRAAIDAQQLWRQVARWRPWVLL
CCEEEEEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHH
LMAGWYEMRWRQSGDRQFITLADEIWCQLDNERKG
HHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA