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The map label for this gene is rumB [H]

Identifier: 152969447

GI number: 152969447

Start: 995105

End: 996232

Strand: Direct

Name: rumB [H]

Synonym: KPN_00890

Alternate gene names: 152969447

Gene position: 995105-996232 (Clockwise)

Preceding gene: 152969446

Following gene: 152969448

Centisome position: 18.72

GC content: 63.56

Gene sequence:

>1128_bases
ATGCATTGCGCACTCTACGACGCCGGCTGTTGCCGCTCCTGTCAATGGCTGGAGCGGCCGGTCCCGCAACAGCTCGCCGA
TAAAATGGCCGATCTGCGCACGCTGCTGGCGGCGATCCCGGTCGCAGCCTGGGGCGAGCCGGTCAGCGGGCCGGAGGCGG
CTTTCCGTAACAAGGCTAAAATGGTGGTCAGCGGCAGCGTTGAGCGTCCGCTGCTGGGGATGCTGTACCGCGATGGCACT
CCGGTGGACCTCACCGACTGCCCGCTCTATCCCGCCGAGTTTGCCCCGGTTTTTGCTGCCCTGAAGCCCTTTATCGCCCG
CGCCGGGTTAACACCTTACAACGTCGCGCGCAAACGCGGGGAGCTGAAATACCTGCTGCTGACCGCCAGCCAGCAGGGGG
AGATGATGCTGCGCTTCGTGCTGCGCTCAGAGGCCAAGGTGGCTCAGCTGCGCGCCGCGCTGCCGTGGCTACAGGCACAG
CTGCCGCAGCTCAGCGTGATCACGGCGAATATCCAGCCGGTGCATATGGCAATCATGGAAGGTGAGCAGGAGATCTTCTT
CACCGAACAGCAGGCGCTGGGGGAGGTCTTTAATGGGGTGCCGCTGTGGATCCGCCCGCAAAGCTTCTTTCAGACCAATC
CGGCGGTAGCCAGCCGGCTGTACGCCACGGCCCGCGACTGGGTGCGCGCGCTGCCGGTCCAGCATATGTGGGATCTGTTT
TGCGGCGTTGGGGGATTTGGCCTGCACTGCGCGACGCCGACGATGCGCCTGACCGGTATCGAGATCGCCCCGGAAGCCAT
CGCCTGCGCCAGACAGTCAGCCGCGCAGCTGGGGCTCAGCAATCTGCATTTTCAGGCGCTGGACTCCACGCAGTTCGCCA
CTCACGAAGCGGATGCCCCGCAGCTGGTGCTGGTTAACCCGCCGCGCCGCGGTATTGGCGCTGAACTATGCGACTATTTA
AGCCGCATGGCGCCGCCCTATATTATCTATTCCAGCTGTAACGCCCGCACCATGGCGACCGATATCGCTCGTCTTAGCGG
CTATCGCGTTGAGCGGGTCCAGCTGTTTGATATGTTCCCCCACACCGCCCACTACGAAGTGCTGACCCTGCTGGTCCGCG
AAGTTTAA

Upstream 100 bases:

>100_bases
TGGTTCTGACGCTGCTGTTCGTGCCGGCGAACTGCCGTCGCTTTTTCCGCCTGCAATAACGGTGATACAATCCCCGCCCC
TGGATTTTTGAAGGTCAATT

Downstream 100 bases:

>100_bases
ACAAAATGTGAAGGCTTTGCGCCGCTGAAATAGGGTAAGCTTGAGGCAGCATGTTTTGTTATTGGTGGGGGGAATCTAAT
GGCCAAAACCATTCTGCTGG

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MHCALYDAGCCRSCQWLERPVPQQLADKMADLRTLLAAIPVAAWGEPVSGPEAAFRNKAKMVVSGSVERPLLGMLYRDGT
PVDLTDCPLYPAEFAPVFAALKPFIARAGLTPYNVARKRGELKYLLLTASQQGEMMLRFVLRSEAKVAQLRAALPWLQAQ
LPQLSVITANIQPVHMAIMEGEQEIFFTEQQALGEVFNGVPLWIRPQSFFQTNPAVASRLYATARDWVRALPVQHMWDLF
CGVGGFGLHCATPTMRLTGIEIAPEAIACARQSAAQLGLSNLHFQALDSTQFATHEADAPQLVLVNPPRRGIGAELCDYL
SRMAPPYIIYSSCNARTMATDIARLSGYRVERVQLFDMFPHTAHYEVLTLLVREV

Sequences:

>Translated_375_residues
MHCALYDAGCCRSCQWLERPVPQQLADKMADLRTLLAAIPVAAWGEPVSGPEAAFRNKAKMVVSGSVERPLLGMLYRDGT
PVDLTDCPLYPAEFAPVFAALKPFIARAGLTPYNVARKRGELKYLLLTASQQGEMMLRFVLRSEAKVAQLRAALPWLQAQ
LPQLSVITANIQPVHMAIMEGEQEIFFTEQQALGEVFNGVPLWIRPQSFFQTNPAVASRLYATARDWVRALPVQHMWDLF
CGVGGFGLHCATPTMRLTGIEIAPEAIACARQSAAQLGLSNLHFQALDSTQFATHEADAPQLVLVNPPRRGIGAELCDYL
SRMAPPYIIYSSCNARTMATDIARLSGYRVERVQLFDMFPHTAHYEVLTLLVREV
>Mature_375_residues
MHCALYDAGCCRSCQWLERPVPQQLADKMADLRTLLAAIPVAAWGEPVSGPEAAFRNKAKMVVSGSVERPLLGMLYRDGT
PVDLTDCPLYPAEFAPVFAALKPFIARAGLTPYNVARKRGELKYLLLTASQQGEMMLRFVLRSEAKVAQLRAALPWLQAQ
LPQLSVITANIQPVHMAIMEGEQEIFFTEQQALGEVFNGVPLWIRPQSFFQTNPAVASRLYATARDWVRALPVQHMWDLF
CGVGGFGLHCATPTMRLTGIEIAPEAIACARQSAAQLGLSNLHFQALDSTQFATHEADAPQLVLVNPPRRGIGAELCDYL
SRMAPPYIIYSSCNARTMATDIARLSGYRVERVQLFDMFPHTAHYEVLTLLVREV

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=190, Percent_Identity=31.0526315789474, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI51173878, Length=190, Percent_Identity=31.0526315789474, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI269784678, Length=389, Percent_Identity=23.1362467866324, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI269784680, Length=355, Percent_Identity=23.3802816901408, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI269784676, Length=355, Percent_Identity=23.3802816901408, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI20149688, Length=355, Percent_Identity=23.3802816901408, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1787083, Length=375, Percent_Identity=80.2666666666667, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=385, Percent_Identity=27.5324675324675, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790403, Length=185, Percent_Identity=28.6486486486486, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24666579, Length=368, Percent_Identity=26.0869565217391, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 41545; Mature: 41545

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCALYDAGCCRSCQWLERPVPQQLADKMADLRTLLAAIPVAAWGEPVSGPEAAFRNKAK
CCEEEECCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCE
MVVSGSVERPLLGMLYRDGTPVDLTDCPLYPAEFAPVFAALKPFIARAGLTPYNVARKRG
EEEECCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYLLLTASQQGEMMLRFVLRSEAKVAQLRAALPWLQAQLPQLSVITANIQPVHMAIME
CEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEC
GEQEIFFTEQQALGEVFNGVPLWIRPQSFFQTNPAVASRLYATARDWVRALPVQHMWDLF
CCCCEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
CGVGGFGLHCATPTMRLTGIEIAPEAIACARQSAAQLGLSNLHFQALDSTQFATHEADAP
HCCCCCCEEECCCCEEEECCEECHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCC
QLVLVNPPRRGIGAELCDYLSRMAPPYIIYSSCNARTMATDIARLSGYRVERVQLFDMFP
EEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEHHCC
HTAHYEVLTLLVREV
CCHHHHHHHHHHHCC
>Mature Secondary Structure
MHCALYDAGCCRSCQWLERPVPQQLADKMADLRTLLAAIPVAAWGEPVSGPEAAFRNKAK
CCEEEECCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCE
MVVSGSVERPLLGMLYRDGTPVDLTDCPLYPAEFAPVFAALKPFIARAGLTPYNVARKRG
EEEECCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYLLLTASQQGEMMLRFVLRSEAKVAQLRAALPWLQAQLPQLSVITANIQPVHMAIME
CEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEC
GEQEIFFTEQQALGEVFNGVPLWIRPQSFFQTNPAVASRLYATARDWVRALPVQHMWDLF
CCCCEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
CGVGGFGLHCATPTMRLTGIEIAPEAIACARQSAAQLGLSNLHFQALDSTQFATHEADAP
HCCCCCCEEECCCCEEEECCEECHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCC
QLVLVNPPRRGIGAELCDYLSRMAPPYIIYSSCNARTMATDIARLSGYRVERVQLFDMFP
EEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEHHCC
HTAHYEVLTLLVREV
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA