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The map label for this gene is purD [H]

Identifier: 150401744

GI number: 150401744

Start: 1384930

End: 1386267

Strand: Direct

Name: purD [H]

Synonym: Maeo_1322

Alternate gene names: 150401744

Gene position: 1384930-1386267 (Clockwise)

Preceding gene: 150401728

Following gene: 150401745

Centisome position: 88.24

GC content: 33.71

Gene sequence:

>1338_bases
ATGAAAATTTTACTTATTGGCGGTGGAGCTCGGGAGCATGCTATTGCTCAGGCATTAAAAAGAAATCCTTCAACAGAATT
ATATACTCTTATGAAAAATAAAAATCCAGGGATATTTGCTATTTCTGAAAAAGTATCATTTAACAGTGAAACCGATTTAG
ATGCAATAAAATCATTTGCGGAAGAAATAAAACCAGAATTAGTAGTTATAGGTCCGGAAGCTCCCCTTGGAGTTGGGGCT
TCGGATATGTTATGGGACATGGGAGCTCCCACAGTAGGTCCAAAAAAATTACCTGCTCAAATTGAGACAAGCAAAGAATT
TATGAGAAATTTATTTAAAAAATACAACATAAAAGGCTCATTAAAATATGGTGCATTTAATCAATATGATGGAGTAGAAG
ACTTTATAGACTCAATGACAGAATTAAATATGGCCGTAGTAGTTAAACCTGCGGGATTAACTGGTGGAAAAGGTGTAAAG
GTAGTAGGGGAGCAATTAAAAGACAACGAAGAAGCAAAAGAATATGCAAAAGAGATATTCGAAAAAAGTATAGGGGGCGG
ACAATTAATAATTGAGGAAAAGCTTGTAGGTGTGGAATTTACACTTCATGGGTTTGTAGATGGAAACAATATTGTATTTA
TGCCACCAGTTCAAGACCATCCTCATGCATATGAAGATGACGAAGGACCTATAACAGGAGGTATGGGTTCATATTCTTGC
CCAGACCATAATTTGCCATTTTTAACAGATAATGACTTAAATAATGCAAAGGAAATAATGAAAAATACCGTAGAGGCAAT
TAAAAAAGAAGTTGGAGAATATAAAGGATTTTTATATGGTCAATTTATGCTAACTGTTGATGGGCCTAAAATAATAGAAT
ATAATGCGAGGTTCGGAGACCCTGAGGCAATGAATTTACTACCCATATTAAAAACCGACTTTGTAGAAGTATGTTTGGCC
ATAGCAAATGGTGAATTAGATAAAATAAATATTGAATTTGACAATAAAGCCACAGTTTGTAAATATCTTGTTCCAGACGG
ATATCCACTTAATCCAGTAAAAGACAAAGAAATATTTGTTGAAGAAGATAAAATTGCCGAAACTGGTGCAATATTAAATT
ATGCTTCAATAAATGAAAAAGATGGTAAATTATATATTACTGGTTCAAGAAGTGCCTCTGTTGTGGGGGTTGCCCCTACA
ATTGAAGAAGCTGAAAAAATTGCAGAAAATGCTGTTCAATATATAAAAGGAGCCGTATTTTATAGGAAAGATATTGGAAC
AGAAAAATTAATTAATAAAAGAATAGTAAGAATGAAGGAGCTCCGGGGCAATAATTAA

Upstream 100 bases:

>100_bases
ATAATATACTATAAAATATAATATACTATAAAAAATATTATAAAACAATGAATATTAATTAAATAGAATTATACTACAAG
TACAAATTAATGGTGATGTT

Downstream 100 bases:

>100_bases
TTAATAATATTATGGTATTTATGCCAATAAAATAATTATATAGTCAATCTTCGGACTATTTCACTAAATCATTTTTTATA
GTTAAACTAAATTTCTTTAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKILLIGGGAREHAIAQALKRNPSTELYTLMKNKNPGIFAISEKVSFNSETDLDAIKSFAEEIKPELVVIGPEAPLGVGA
SDMLWDMGAPTVGPKKLPAQIETSKEFMRNLFKKYNIKGSLKYGAFNQYDGVEDFIDSMTELNMAVVVKPAGLTGGKGVK
VVGEQLKDNEEAKEYAKEIFEKSIGGGQLIIEEKLVGVEFTLHGFVDGNNIVFMPPVQDHPHAYEDDEGPITGGMGSYSC
PDHNLPFLTDNDLNNAKEIMKNTVEAIKKEVGEYKGFLYGQFMLTVDGPKIIEYNARFGDPEAMNLLPILKTDFVEVCLA
IANGELDKINIEFDNKATVCKYLVPDGYPLNPVKDKEIFVEEDKIAETGAILNYASINEKDGKLYITGSRSASVVGVAPT
IEEAEKIAENAVQYIKGAVFYRKDIGTEKLINKRIVRMKELRGNN

Sequences:

>Translated_445_residues
MKILLIGGGAREHAIAQALKRNPSTELYTLMKNKNPGIFAISEKVSFNSETDLDAIKSFAEEIKPELVVIGPEAPLGVGA
SDMLWDMGAPTVGPKKLPAQIETSKEFMRNLFKKYNIKGSLKYGAFNQYDGVEDFIDSMTELNMAVVVKPAGLTGGKGVK
VVGEQLKDNEEAKEYAKEIFEKSIGGGQLIIEEKLVGVEFTLHGFVDGNNIVFMPPVQDHPHAYEDDEGPITGGMGSYSC
PDHNLPFLTDNDLNNAKEIMKNTVEAIKKEVGEYKGFLYGQFMLTVDGPKIIEYNARFGDPEAMNLLPILKTDFVEVCLA
IANGELDKINIEFDNKATVCKYLVPDGYPLNPVKDKEIFVEEDKIAETGAILNYASINEKDGKLYITGSRSASVVGVAPT
IEEAEKIAENAVQYIKGAVFYRKDIGTEKLINKRIVRMKELRGNN
>Mature_445_residues
MKILLIGGGAREHAIAQALKRNPSTELYTLMKNKNPGIFAISEKVSFNSETDLDAIKSFAEEIKPELVVIGPEAPLGVGA
SDMLWDMGAPTVGPKKLPAQIETSKEFMRNLFKKYNIKGSLKYGAFNQYDGVEDFIDSMTELNMAVVVKPAGLTGGKGVK
VVGEQLKDNEEAKEYAKEIFEKSIGGGQLIIEEKLVGVEFTLHGFVDGNNIVFMPPVQDHPHAYEDDEGPITGGMGSYSC
PDHNLPFLTDNDLNNAKEIMKNTVEAIKKEVGEYKGFLYGQFMLTVDGPKIIEYNARFGDPEAMNLLPILKTDFVEVCLA
IANGELDKINIEFDNKATVCKYLVPDGYPLNPVKDKEIFVEEDKIAETGAILNYASINEKDGKLYITGSRSASVVGVAPT
IEEAEKIAENAVQYIKGAVFYRKDIGTEKLINKRIVRMKELRGNN

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=436, Percent_Identity=36.0091743119266, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI209869995, Length=436, Percent_Identity=36.0091743119266, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI209869993, Length=436, Percent_Identity=36.0091743119266, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI28416899, Length=436, Percent_Identity=36.0091743119266, Blast_Score=218, Evalue=1e-56,
Organism=Escherichia coli, GI1790438, Length=436, Percent_Identity=32.5688073394495, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17567511, Length=442, Percent_Identity=31.6742081447964, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321203, Length=455, Percent_Identity=29.8901098901099, Blast_Score=176, Evalue=5e-45,
Organism=Drosophila melanogaster, GI62471619, Length=363, Percent_Identity=36.0881542699724, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24582400, Length=360, Percent_Identity=36.1111111111111, Blast_Score=188, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 49007; Mature: 49007

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLIGGGAREHAIAQALKRNPSTELYTLMKNKNPGIFAISEKVSFNSETDLDAIKSFA
CEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHH
EEIKPELVVIGPEAPLGVGASDMLWDMGAPTVGPKKLPAQIETSKEFMRNLFKKYNIKGS
HHCCCCEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
LKYGAFNQYDGVEDFIDSMTELNMAVVVKPAGLTGGKGVKVVGEQLKDNEEAKEYAKEIF
EEECCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
EKSIGGGQLIIEEKLVGVEFTLHGFVDGNNIVFMPPVQDHPHAYEDDEGPITGGMGSYSC
HHCCCCCEEEEEEEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCEECCCCCCCC
PDHNLPFLTDNDLNNAKEIMKNTVEAIKKEVGEYKGFLYGQFMLTVDGPKIIEYNARFGD
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCEEEEECCCCCC
PEAMNLLPILKTDFVEVCLAIANGELDKINIEFDNKATVCKYLVPDGYPLNPVKDKEIFV
CCHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEE
EEDKIAETGAILNYASINEKDGKLYITGSRSASVVGVAPTIEEAEKIAENAVQYIKGAVF
ECCHHHHCCCEEEEECCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHE
YRKDIGTEKLINKRIVRMKELRGNN
EECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKILLIGGGAREHAIAQALKRNPSTELYTLMKNKNPGIFAISEKVSFNSETDLDAIKSFA
CEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHH
EEIKPELVVIGPEAPLGVGASDMLWDMGAPTVGPKKLPAQIETSKEFMRNLFKKYNIKGS
HHCCCCEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
LKYGAFNQYDGVEDFIDSMTELNMAVVVKPAGLTGGKGVKVVGEQLKDNEEAKEYAKEIF
EEECCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
EKSIGGGQLIIEEKLVGVEFTLHGFVDGNNIVFMPPVQDHPHAYEDDEGPITGGMGSYSC
HHCCCCCEEEEEEEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCEECCCCCCCC
PDHNLPFLTDNDLNNAKEIMKNTVEAIKKEVGEYKGFLYGQFMLTVDGPKIIEYNARFGD
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCEEEEECCCCCC
PEAMNLLPILKTDFVEVCLAIANGELDKINIEFDNKATVCKYLVPDGYPLNPVKDKEIFV
CCHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEE
EEDKIAETGAILNYASINEKDGKLYITGSRSASVVGVAPTIEEAEKIAENAVQYIKGAVF
ECCHHHHCCCEEEEECCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHE
YRKDIGTEKLINKRIVRMKELRGNN
EECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA