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The map label for this gene is vhuA [H]

Identifier: 150401638

GI number: 150401638

Start: 1285097

End: 1286353

Strand: Direct

Name: vhuA [H]

Synonym: Maeo_1215

Alternate gene names: 150401638

Gene position: 1285097-1286353 (Clockwise)

Preceding gene: 150401637

Following gene: 150401639

Centisome position: 81.88

GC content: 37.71

Gene sequence:

>1257_bases
ATGAGTAAAATTACAATTGAACCAATATCCCGAATTGAAGGGCATGGGAAAGTTACAATTACATTGGACGACTCAGGAGC
TCCCACCGATGTTAAGTTGCACATAACAGCACTAAGAGGATTTGAACAATTTGTTGTTGGAAGACCCGCAGAGGAGGTTC
CAAGGATTGTCCCAAGAATCTGCGGTATATGTCAAACTCCACATCACTTAGCATCCGTTAAAGCTGTTGAGGCAGCATGG
GATGCTGAGGTTCCAAGTGCAGCTAACAAAGTGAGGGAATTAATGCATTTAGGAAATATGATTCACAGTCATTCATTACA
TTTCTATTTCCTGGCTGCTCCTGATTTTGTAATGGGCCCAGATTCAAACCCCGCTACAAGAAACATAGTTGGAGTAATCG
GGGAAGCTCCTGATGTCGCAAAACAGGCAATAGCTTTAAGAAAATTTGGTCAAACCATAGTAGCAACCACCGGGGGAAGA
CCAATACACCCGGTAACAGGTATTCCAGGGGGATTGTCAAAATCATTTGCAGAAGCTGATAGAGATGCATTTTTGGCTGA
AGTAGATACAATGATTGAATATGCTCAAAATGGAGTAGCATTAATTAATTCATTAAATGAAAAATACGCAGATGCTACTA
AATCATTGGGTGTAATTGATACTTGGTATTTAGGAATGGTTAAAGATGGAAAACATAATTTCTATGAAGATACACTTAGA
TTTGTATCTCCTGATGGAAAAACAAAACAAGAATTCAAACCAGCCGAATATCTTGATAATATTGGAGAGCATGTTGTAGA
ACACAGCTATTCAAAATATCCATACAATAAAAAAGTAGGATATCCTGATGGATTATATAGGGTAGGTCCATTGCCAATGC
TAAATGTATGTGATAGTATGGCAACACCACTTGCAGAAGAAGCAAGAAAAGAATTTGTTGAAACATTCGGAGCTCCTGCA
AATCAATCATTAGCTTACAACCATGCAAGATTAATAGAAATATTGGCAGCATGCGAAAGAGTTAAAGAATTGTTAAATGA
TGGAGAAATTACTTCAACCGATGTTAAAGCAGAAGTTCAACCAAAGGCAGGAAATGGTGTTGGAGCAATATATGCACCAA
GAGGAACGCTATTCCACAACTATGAAACAGATGACAATGGAATAGTTATAAAAGGTAATATGATTGTTGCTACTACCCAC
AATGTGCCAACAATGGAAAAGGCAATTCAACAAGCTGCAAAAGAAATATTTAAATAA

Upstream 100 bases:

>100_bases
ATCTGCCATATTTATATTGGCGACCATTATTGGAAGATAATTTTATAATTATTTAATCTCATTATATTTATATGTTATAA
TTAATACACGGTGAATCCAA

Downstream 100 bases:

>100_bases
ATCTCAAAATTTTCGGAGAAAATTTTGAATTTTGAAATATGGAGATAATTGATTATTATATATATTATTTGCACATTGGT
AAATTATCAATTAATGAATA

Product: nickel-dependent hydrogenase large subunit

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSKITIEPISRIEGHGKVTITLDDSGAPTDVKLHITALRGFEQFVVGRPAEEVPRIVPRICGICQTPHHLASVKAVEAAW
DAEVPSAANKVRELMHLGNMIHSHSLHFYFLAAPDFVMGPDSNPATRNIVGVIGEAPDVAKQAIALRKFGQTIVATTGGR
PIHPVTGIPGGLSKSFAEADRDAFLAEVDTMIEYAQNGVALINSLNEKYADATKSLGVIDTWYLGMVKDGKHNFYEDTLR
FVSPDGKTKQEFKPAEYLDNIGEHVVEHSYSKYPYNKKVGYPDGLYRVGPLPMLNVCDSMATPLAEEARKEFVETFGAPA
NQSLAYNHARLIEILAACERVKELLNDGEITSTDVKAEVQPKAGNGVGAIYAPRGTLFHNYETDDNGIVIKGNMIVATTH
NVPTMEKAIQQAAKEIFK

Sequences:

>Translated_418_residues
MSKITIEPISRIEGHGKVTITLDDSGAPTDVKLHITALRGFEQFVVGRPAEEVPRIVPRICGICQTPHHLASVKAVEAAW
DAEVPSAANKVRELMHLGNMIHSHSLHFYFLAAPDFVMGPDSNPATRNIVGVIGEAPDVAKQAIALRKFGQTIVATTGGR
PIHPVTGIPGGLSKSFAEADRDAFLAEVDTMIEYAQNGVALINSLNEKYADATKSLGVIDTWYLGMVKDGKHNFYEDTLR
FVSPDGKTKQEFKPAEYLDNIGEHVVEHSYSKYPYNKKVGYPDGLYRVGPLPMLNVCDSMATPLAEEARKEFVETFGAPA
NQSLAYNHARLIEILAACERVKELLNDGEITSTDVKAEVQPKAGNGVGAIYAPRGTLFHNYETDDNGIVIKGNMIVATTH
NVPTMEKAIQQAAKEIFK
>Mature_417_residues
SKITIEPISRIEGHGKVTITLDDSGAPTDVKLHITALRGFEQFVVGRPAEEVPRIVPRICGICQTPHHLASVKAVEAAWD
AEVPSAANKVRELMHLGNMIHSHSLHFYFLAAPDFVMGPDSNPATRNIVGVIGEAPDVAKQAIALRKFGQTIVATTGGRP
IHPVTGIPGGLSKSFAEADRDAFLAEVDTMIEYAQNGVALINSLNEKYADATKSLGVIDTWYLGMVKDGKHNFYEDTLRF
VSPDGKTKQEFKPAEYLDNIGEHVVEHSYSKYPYNKKVGYPDGLYRVGPLPMLNVCDSMATPLAEEARKEFVETFGAPAN
QSLAYNHARLIEILAACERVKELLNDGEITSTDVKAEVQPKAGNGVGAIYAPRGTLFHNYETDDNGIVIKGNMIVATTHN
VPTMEKAIQQAAKEIFK

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=115, Percent_Identity=38.2608695652174, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787207, Length=115, Percent_Identity=37.3913043478261, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.98.1

Molecular weight: Translated: 45591; Mature: 45460

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITIEPISRIEGHGKVTITLDDSGAPTDVKLHITALRGFEQFVVGRPAEEVPRIVPRI
CCCEEECHHHHCCCCCEEEEEECCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHHHHHH
CGICQTPHHLASVKAVEAAWDAEVPSAANKVRELMHLGNMIHSHSLHFYFLAAPDFVMGP
HHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEECC
DSNPATRNIVGVIGEAPDVAKQAIALRKFGQTIVATTGGRPIHPVTGIPGGLSKSFAEAD
CCCCCCHHHHEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHH
RDAFLAEVDTMIEYAQNGVALINSLNEKYADATKSLGVIDTWYLGMVKDGKHNFYEDTLR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCHHHHHHHH
FVSPDGKTKQEFKPAEYLDNIGEHVVEHSYSKYPYNKKVGYPDGLYRVGPLPMLNVCDSM
HCCCCCCCHHHCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEECCCCCHHHHHHHH
ATPLAEEARKEFVETFGAPANQSLAYNHARLIEILAACERVKELLNDGEITSTDVKAEVQ
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEC
PKAGNGVGAIYAPRGTLFHNYETDDNGIVIKGNMIVATTHNVPTMEKAIQQAAKEIFK
CCCCCCCEEEEECCCCEEECCCCCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKITIEPISRIEGHGKVTITLDDSGAPTDVKLHITALRGFEQFVVGRPAEEVPRIVPRI
CCEEECHHHHCCCCCEEEEEECCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHHHHHH
CGICQTPHHLASVKAVEAAWDAEVPSAANKVRELMHLGNMIHSHSLHFYFLAAPDFVMGP
HHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEECC
DSNPATRNIVGVIGEAPDVAKQAIALRKFGQTIVATTGGRPIHPVTGIPGGLSKSFAEAD
CCCCCCHHHHEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHH
RDAFLAEVDTMIEYAQNGVALINSLNEKYADATKSLGVIDTWYLGMVKDGKHNFYEDTLR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCHHHHHHHH
FVSPDGKTKQEFKPAEYLDNIGEHVVEHSYSKYPYNKKVGYPDGLYRVGPLPMLNVCDSM
HCCCCCCCHHHCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEECCCCCHHHHHHHH
ATPLAEEARKEFVETFGAPANQSLAYNHARLIEILAACERVKELLNDGEITSTDVKAEVQ
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEC
PKAGNGVGAIYAPRGTLFHNYETDDNGIVIKGNMIVATTHNVPTMEKAIQQAAKEIFK
CCCCCCCEEEEECCCCEEECCCCCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1603063 [H]