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The map label for this gene is hisA

Identifier: 150401623

GI number: 150401623

Start: 1268895

End: 1269620

Strand: Direct

Name: hisA

Synonym: Maeo_1200

Alternate gene names: 150401623

Gene position: 1268895-1269620 (Clockwise)

Preceding gene: 150401622

Following gene: 150401624

Centisome position: 80.85

GC content: 33.2

Gene sequence:

>726_bases
ATGATAGTAATTCCAGCAGTAGATATAAAAAATAAAAAATGTGTTCAGTTGATACAGGGAAATCCTGATAAAAAACATAT
AGAATTAGATAATCCATTGGAAGTTGCAGAAAAATGGATAAAATGCGGGGCTGAAATGATACATTTGGTTGATTTAGATA
GTGCCATATATGGAACTGATACAAATAAAGAAACCATAAAAAAAATTATAGAATCTGTTCCCGTTCCCGTTGAAGTGGGC
GGAGGGGTAAGAACCGTAGAAGATGCCCTAAATTTAATAGACCTTGGAGCAGAACGAGTAATATTGGGGACATCGGCTGT
AAAAAATCCAGAGATAATAACGGAGCTCCTTGAAAAAGGAATAAATTCTGAAAAGATAATGGTGGCGTTGGATGCAAAAG
ATGGAAAAGTTGTAATAAAGGGTTGGATGGAGAAAACAGAATATACTCCAATAGAAATTGGTAAAATACTTGAAGAAAAA
GGTGCAGGAAGTATATTATTTACTAATGTAGATGTAGAAGGACTATTGGACGGAATAGACATTGAACCTGTTAAAAAATT
AGTAGATGAGTTAAATATTCCTATAATTGCATCTGGGGGAATAACTACATATGACGACCTTCTGAAATTAAAAGAAATTG
GGGTTGATGGTGTTGTTGTTGGTTCTGCAATATATAAGGAAATGATGGATTTGAAAAAAGCAATAAAAATAGTAAAAGGT
GAATAA

Upstream 100 bases:

>100_bases
GATTGACTATGCAGTATATTCGGAGCTCCAATATAACAATAATATCAATACCAATATTAAATTAATATTAAATCTTTTCA
AACACACGCATGGTGAAAAT

Downstream 100 bases:

>100_bases
ATGAATAATAAAATAATAACTGTTGGACATATAGCATTGGATTATATATTTAATGTAGCGGAGCTCCCTAAACCAAATAC
CTCCGTTCAAATACCTACGG

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MIVIPAVDIKNKKCVQLIQGNPDKKHIELDNPLEVAEKWIKCGAEMIHLVDLDSAIYGTDTNKETIKKIIESVPVPVEVG
GGVRTVEDALNLIDLGAERVILGTSAVKNPEIITELLEKGINSEKIMVALDAKDGKVVIKGWMEKTEYTPIEIGKILEEK
GAGSILFTNVDVEGLLDGIDIEPVKKLVDELNIPIIASGGITTYDDLLKLKEIGVDGVVVGSAIYKEMMDLKKAIKIVKG
E

Sequences:

>Translated_241_residues
MIVIPAVDIKNKKCVQLIQGNPDKKHIELDNPLEVAEKWIKCGAEMIHLVDLDSAIYGTDTNKETIKKIIESVPVPVEVG
GGVRTVEDALNLIDLGAERVILGTSAVKNPEIITELLEKGINSEKIMVALDAKDGKVVIKGWMEKTEYTPIEIGKILEEK
GAGSILFTNVDVEGLLDGIDIEPVKKLVDELNIPIIASGGITTYDDLLKLKEIGVDGVVVGSAIYKEMMDLKKAIKIVKG
E
>Mature_241_residues
MIVIPAVDIKNKKCVQLIQGNPDKKHIELDNPLEVAEKWIKCGAEMIHLVDLDSAIYGTDTNKETIKKIIESVPVPVEVG
GGVRTVEDALNLIDLGAERVILGTSAVKNPEIITELLEKGINSEKIMVALDAKDGKVVIKGWMEKTEYTPIEIGKILEEK
GAGSILFTNVDVEGLLDGIDIEPVKKLVDELNIPIIASGGITTYDDLLKLKEIGVDGVVVGSAIYKEMMDLKKAIKIVKG
E

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=30.4166666666667, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788336, Length=240, Percent_Identity=23.75, Blast_Score=100, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_META3 (A6UWA5)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325389.1
- ProteinModelPortal:   A6UWA5
- SMR:   A6UWA5
- STRING:   A6UWA5
- GeneID:   5327464
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1200
- eggNOG:   arNOG08089
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK13585
- BioCyc:   MAEO419665:MAEO_1200-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26177; Mature: 26177

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIPAVDIKNKKCVQLIQGNPDKKHIELDNPLEVAEKWIKCGAEMIHLVDLDSAIYGTD
CEEECCCCCCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCHHEEEEEECCCCEECCC
TNKETIKKIIESVPVPVEVGGGVRTVEDALNLIDLGAERVILGTSAVKNPEIITELLEKG
CCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
INSEKIMVALDAKDGKVVIKGWMEKTEYTPIEIGKILEEKGAGSILFTNVDVEGLLDGID
CCCCEEEEEEECCCCEEEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCC
IEPVKKLVDELNIPIIASGGITTYDDLLKLKEIGVDGVVVGSAIYKEMMDLKKAIKIVKG
HHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure
MIVIPAVDIKNKKCVQLIQGNPDKKHIELDNPLEVAEKWIKCGAEMIHLVDLDSAIYGTD
CEEECCCCCCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCHHEEEEEECCCCEECCC
TNKETIKKIIESVPVPVEVGGGVRTVEDALNLIDLGAERVILGTSAVKNPEIITELLEKG
CCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
INSEKIMVALDAKDGKVVIKGWMEKTEYTPIEIGKILEEKGAGSILFTNVDVEGLLDGID
CCCCEEEEEEECCCCEEEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCC
IEPVKKLVDELNIPIIASGGITTYDDLLKLKEIGVDGVVVGSAIYKEMMDLKKAIKIVKG
HHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA