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The map label for this gene is trpB

Identifier: 150401543

GI number: 150401543

Start: 1165132

End: 1166301

Strand: Direct

Name: trpB

Synonym: Maeo_1120

Alternate gene names: 150401543

Gene position: 1165132-1166301 (Clockwise)

Preceding gene: 150401542

Following gene: 150401544

Centisome position: 74.24

GC content: 36.07

Gene sequence:

>1170_bases
ATGGAATATAAATTCGGAGAATATGGAGGTCAATATGTGCCTGAGGTGCTTATGCCGTCATTAAAAGAATTGGAAAAAGC
ATATAAAAAATACAAAGATGACCCAGAATTTAAAGAAGAGCTTGAATACTACTTAAAACAATATGCTGGAAGGGAGACCC
CATTATATTTTGCTGAAAACCTAACAAAAAAGATGGGAGGAGCTAAAATATACCTAAAAAGAGAGGATTTATTATTGGGG
GGAGCTCACAAAATAAACAATAGTTTAGGACAGGCACTACTTGCTAAAAGAATAGGCAAAACAAGAATTATTGCCGAAAC
AGGAGCAGGAGAACATGGGCTATCTACGGCAATGGTTGGAGCTCTATTTGGACTTAAAGCTAAAATATATATGGGAGCAG
TAGATGTTGAAAGACAAAAATTAAATGTTTATAAAATGAGGTTGCACGGTGCAGAGGTTCATGCCGTTCAATCTGGTTCA
AAAACATTAAAAGATGCCATAAATGAAGCACTTAGAGATTGGGTTGAGACCTTTGAAGATACCCATTATATTATTGGTTC
AGCTGTTGGTCCTTATCCATTCCCTTCAATGGTTAGAGATTTCCAGTCAGTAATTGGTAAAGAAGCTAAAAAGCAAATAT
TAGAGGCAGAAGGACGACTTCCAGATAGTATTGTAGCTTGTGTGGGGGGAGGAAGTAATTCTATTGGAATATTTAATGAA
TTTAAACAGGATAAAGAAGTTAAATTAATTGGTGTTGAGGCAGCGGGTGAAGGACTTGACACAGATAGGCACGGAGCAGC
AATATTAAAAGGTAAAAAGGGAGTTTTACATGGTATGCTATCCAAGTTTTTACAAGATGATGATGGACAAATAGCCGAAA
CATACAGTATTTCCGCAGGTTTAGATTATCCAGGAGTAGGTCCAGAACATGCTTATTTAGATGAAATTAAAAGAGTAGAA
TATGCAGGAATTACCGATGTTGAGGCACTTGATGCTTTTTCAACATTGTCAAAAACCGAAGGAATTATCCCAGCTCTTGA
ATCTTCCCATGCTGTTGCCCATGGTATGAAAATTGCCAAAGAAATGGATAAAGATGAAATAATTATTATTAATTTATCGG
GCAGAGGAGATAAAGACATACATACCGTTATGAATTTCATTGAGTTTTAA

Upstream 100 bases:

>100_bases
TGATAAATAAAATATTATTAATTATAATATAATAAGAATATATAATGAATAATTATAATAAATGATTATAAAATTTATTT
AATAAATAATCGGTGATGTT

Downstream 100 bases:

>100_bases
TATATGATATATAATATTATCTATTTATAATCCATTATTATTTATTCATATCATCATCATTGTAAAATATGTGTTATTAT
AAATACAATTATTAAAAGTG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MEYKFGEYGGQYVPEVLMPSLKELEKAYKKYKDDPEFKEELEYYLKQYAGRETPLYFAENLTKKMGGAKIYLKREDLLLG
GAHKINNSLGQALLAKRIGKTRIIAETGAGEHGLSTAMVGALFGLKAKIYMGAVDVERQKLNVYKMRLHGAEVHAVQSGS
KTLKDAINEALRDWVETFEDTHYIIGSAVGPYPFPSMVRDFQSVIGKEAKKQILEAEGRLPDSIVACVGGGSNSIGIFNE
FKQDKEVKLIGVEAAGEGLDTDRHGAAILKGKKGVLHGMLSKFLQDDDGQIAETYSISAGLDYPGVGPEHAYLDEIKRVE
YAGITDVEALDAFSTLSKTEGIIPALESSHAVAHGMKIAKEMDKDEIIIINLSGRGDKDIHTVMNFIEF

Sequences:

>Translated_389_residues
MEYKFGEYGGQYVPEVLMPSLKELEKAYKKYKDDPEFKEELEYYLKQYAGRETPLYFAENLTKKMGGAKIYLKREDLLLG
GAHKINNSLGQALLAKRIGKTRIIAETGAGEHGLSTAMVGALFGLKAKIYMGAVDVERQKLNVYKMRLHGAEVHAVQSGS
KTLKDAINEALRDWVETFEDTHYIIGSAVGPYPFPSMVRDFQSVIGKEAKKQILEAEGRLPDSIVACVGGGSNSIGIFNE
FKQDKEVKLIGVEAAGEGLDTDRHGAAILKGKKGVLHGMLSKFLQDDDGQIAETYSISAGLDYPGVGPEHAYLDEIKRVE
YAGITDVEALDAFSTLSKTEGIIPALESSHAVAHGMKIAKEMDKDEIIIINLSGRGDKDIHTVMNFIEF
>Mature_389_residues
MEYKFGEYGGQYVPEVLMPSLKELEKAYKKYKDDPEFKEELEYYLKQYAGRETPLYFAENLTKKMGGAKIYLKREDLLLG
GAHKINNSLGQALLAKRIGKTRIIAETGAGEHGLSTAMVGALFGLKAKIYMGAVDVERQKLNVYKMRLHGAEVHAVQSGS
KTLKDAINEALRDWVETFEDTHYIIGSAVGPYPFPSMVRDFQSVIGKEAKKQILEAEGRLPDSIVACVGGGSNSIGIFNE
FKQDKEVKLIGVEAAGEGLDTDRHGAAILKGKKGVLHGMLSKFLQDDDGQIAETYSISAGLDYPGVGPEHAYLDEIKRVE
YAGITDVEALDAFSTLSKTEGIIPALESSHAVAHGMKIAKEMDKDEIIIINLSGRGDKDIHTVMNFIEF

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=385, Percent_Identity=58.4415584415584, Blast_Score=475, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6321412, Length=381, Percent_Identity=54.8556430446194, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_META3 (A6UW25)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325309.1
- ProteinModelPortal:   A6UW25
- SMR:   A6UW25
- STRING:   A6UW25
- GeneID:   5327416
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1120
- eggNOG:   arNOG04577
- HOGENOM:   HBG303148
- OMA:   TFENTHY
- ProtClustDB:   PRK04346
- BioCyc:   MAEO419665:MAEO_1120-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 42775; Mature: 42775

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKFGEYGGQYVPEVLMPSLKELEKAYKKYKDDPEFKEELEYYLKQYAGRETPLYFAEN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
LTKKMGGAKIYLKREDLLLGGAHKINNSLGQALLAKRIGKTRIIAETGAGEHGLSTAMVG
HHHHCCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
ALFGLKAKIYMGAVDVERQKLNVYKMRLHGAEVHAVQSGSKTLKDAINEALRDWVETFED
HHHHHHHHEEECCCHHHHHHHHEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHCC
THYIIGSAVGPYPFPSMVRDFQSVIGKEAKKQILEAEGRLPDSIVACVGGGSNSIGIFNE
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCHHHH
FKQDKEVKLIGVEAAGEGLDTDRHGAAILKGKKGVLHGMLSKFLQDDDGQIAETYSISAG
HCCCCCEEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEECC
LDYPGVGPEHAYLDEIKRVEYAGITDVEALDAFSTLSKTEGIIPALESSHAVAHGMKIAK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
EMDKDEIIIINLSGRGDKDIHTVMNFIEF
HCCCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MEYKFGEYGGQYVPEVLMPSLKELEKAYKKYKDDPEFKEELEYYLKQYAGRETPLYFAEN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
LTKKMGGAKIYLKREDLLLGGAHKINNSLGQALLAKRIGKTRIIAETGAGEHGLSTAMVG
HHHHCCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
ALFGLKAKIYMGAVDVERQKLNVYKMRLHGAEVHAVQSGSKTLKDAINEALRDWVETFED
HHHHHHHHEEECCCHHHHHHHHEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHCC
THYIIGSAVGPYPFPSMVRDFQSVIGKEAKKQILEAEGRLPDSIVACVGGGSNSIGIFNE
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCHHHH
FKQDKEVKLIGVEAAGEGLDTDRHGAAILKGKKGVLHGMLSKFLQDDDGQIAETYSISAG
HCCCCCEEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEECC
LDYPGVGPEHAYLDEIKRVEYAGITDVEALDAFSTLSKTEGIIPALESSHAVAHGMKIAK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
EMDKDEIIIINLSGRGDKDIHTVMNFIEF
HCCCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA