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The map label for this gene is ppsA [H]

Identifier: 150400706

GI number: 150400706

Start: 296426

End: 298735

Strand: Direct

Name: ppsA [H]

Synonym: Maeo_0271

Alternate gene names: 150400706

Gene position: 296426-298735 (Clockwise)

Preceding gene: 150400704

Following gene: 150400707

Centisome position: 18.89

GC content: 36.54

Gene sequence:

>2310_bases
ATGACATTAATCGCATGGTTAGATGACCTTTCAAATGAAGATGTGAATATTGCAGGAGGAAAAGGAGCTTCGCTTGGTGA
AATGTGGAATGCGGGGTTGCCTGTTCCCCCAGCATTTGTAGTAACCGCAGAAGCATATAGATATTTTATAAATGAAACGA
AATTAAATAATAAAATAATGGAAGTTTTAGAAGGGCTTGATGTTAATAACAATGACGAATTAACTAAAAAATCAGCAGAA
GTGAGAAAATTAATAGAATCTGCTGAAATGCCAACTGATTTAAAAATTGCCATTGTAGAAAGCTATAGTAAATTAGGGCA
AATGACTGGTACTGATGAAACATTTGTAGCAGTTAGGAGCTCCGCCACAGCTGAGGATTTACCTGATGCAAGTTTTGCAG
GGCAACAAGATACATATTTAAATATGAAAGGAGCTGATGATGTAGTAGATGCCGTTCAAAGATGTTTTGCATCATTATTT
ACTCCAAGAGCTGTATTTTATAGGGAACAGAAAGGATTTGACCACTTTGAGGTTGCGTTAGCTGCAGTAGTTCAAAAAAT
GGTAAATGCCGATAAAGCAGGTGTTATGTTTACTGTAAATCCCATAAACCACAACTACGATGAAATTGTAATCGAAGGAG
CTTGGGGATTGGGAGAAGGTGTTGTTAGTGGAACAGTTAGTCCAGATACATATATTGTTAGCAAGGATAAAAAAATATTG
GATGTATATATTGCTACAAAAGAAACAATGTTTGAAAAGGACGAAAATGGAATAACAAGAGAAATTCCAACACCTGATGA
ATTAAAAGATAAAAGAGTATTGTCAGATGAAGAATTATTGAAATTAGTAGATAAAGGATTAGTAATTGAAAAACATTACA
ATAGACCAATGGACATAGAGTGGGCATTGGAAAATGAGGGCGTATATATGCTTCAAGCAAGGCCAATTACTACGCTAGAT
GAAAAACCAGCAAAAACAGGAGGGGGAGCAAAACCTCCAAGTTCAGAAGATGCTGGTGGAAATATAATTTTAAAAGGAAT
TGGAGCATCACCTGGAACAGCATCAGGAACTGTAAAAATATTAAATAAAATTGAAGAAATTGATAAAATTAAAGAAGGCG
ACATATTAGTTACAAAAATGACAACTCCCGACATGGTCCCCGCAATGAAAAAAGCAAGTGCTATTGTTACCAATGATGGA
GGATTGACATGTCACGCTGCAATCATATCTCGAGAATTGGGAACACCTTGTGTAGTCGGAACTAAAAAGGCCACGGATGT
TTTAAAAGAAGGCACCATTATCACCATAGATGGTGAAAAAGGAGTGGTTTATGAAGGAGAATTGGCTAAAAAAGAAGAAG
CAGTTCAAGCCAGAGTGGTTTATCAGGGAGCTCCCCTTATCACAGCAACAGAAATAAAGGTAAATGTCTCTATGCCAGAA
GTTGCTGAAAGAGCAGCCGCAACTAATGCGGATGGAGTAGGGCTTTTAAGAGCAGAACATATGATATTAGGAACAGGAGT
TCATCCATTGAAAGTATTGAAAGATAAAGGAGAAGAAGGTCTAATAAATGTATTTGCCGATGGAATTAGAGTTGTGGCAG
ATGCATTCTATCCAAGAACCATTACTTACAGAACCCTCGATGCCCCAACAGATGAATTTAGAGGATTAGAAGGTGGAGAG
GACGAGCCAGAAGAGCACAACCCAATGCTTGGATGGAGAGGAATTAGAAGAGGATTAGACCAGCCTGAAATATTAAGATG
TGAATTATTGGCAGTTAAAAAATTGCTTAATGAAGGATATAAAAATATAATGGTCATGATTCCTCTGGTATCAAGTGTAG
ATGAAATTAAAAAAGTTAGGAAGCTTGCGGATACCGTAGGTTTAAGACTTGGAAAAGATGTAGAATTTGGAGTTATGGTC
GAAACTCCTGCGGCAGCTCTAATTATCGAAGATATAGTAGAAACTGATATCGACTTTGTGAGTTTGGGAACAAATGATTT
GACCCAATATACAATTGCTATCGATAGAAATAACGAATATGTTGCTAAATATTATAGGGAAAATCACCCTGCTGTATTGA
AATTAATAGAGCATGTGATAAAAACCTGTAAAAAGAAAGGAGTAAAAACATCAATTTGTGGTCAAGCTGGAAGCAGACCT
GCAATTGTGGAAAAACTCGTAGAATGGGGGATTACAAGCATATCCTCAAATATTGATGCCATAGATACAATTAGGCAGAC
TGTGGCAAGAACAGAACAGAGAATTATTCTTGACATTATAAGAGATAGTAAAAATAGAAGTGGATTGTAA

Upstream 100 bases:

>100_bases
TATGAGCTATATAATATAGGTGCACATATAATTATAAGATAATATTAAAAACTATGAGCTACATAGTATATAGTTCCCAT
ATCAAGAGAGGAGAAAAAAT

Downstream 100 bases:

>100_bases
ATATATTAAATAAAAAATAATAAATAAAATTTCTTTTTTTATCCTGAGCAAAGCGAAGGATATAGAAAATCCTTGTGATT
TTTTTTATTTTTTTAATTTT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 769; Mature: 768

Protein sequence:

>769_residues
MTLIAWLDDLSNEDVNIAGGKGASLGEMWNAGLPVPPAFVVTAEAYRYFINETKLNNKIMEVLEGLDVNNNDELTKKSAE
VRKLIESAEMPTDLKIAIVESYSKLGQMTGTDETFVAVRSSATAEDLPDASFAGQQDTYLNMKGADDVVDAVQRCFASLF
TPRAVFYREQKGFDHFEVALAAVVQKMVNADKAGVMFTVNPINHNYDEIVIEGAWGLGEGVVSGTVSPDTYIVSKDKKIL
DVYIATKETMFEKDENGITREIPTPDELKDKRVLSDEELLKLVDKGLVIEKHYNRPMDIEWALENEGVYMLQARPITTLD
EKPAKTGGGAKPPSSEDAGGNIILKGIGASPGTASGTVKILNKIEEIDKIKEGDILVTKMTTPDMVPAMKKASAIVTNDG
GLTCHAAIISRELGTPCVVGTKKATDVLKEGTIITIDGEKGVVYEGELAKKEEAVQARVVYQGAPLITATEIKVNVSMPE
VAERAAATNADGVGLLRAEHMILGTGVHPLKVLKDKGEEGLINVFADGIRVVADAFYPRTITYRTLDAPTDEFRGLEGGE
DEPEEHNPMLGWRGIRRGLDQPEILRCELLAVKKLLNEGYKNIMVMIPLVSSVDEIKKVRKLADTVGLRLGKDVEFGVMV
ETPAAALIIEDIVETDIDFVSLGTNDLTQYTIAIDRNNEYVAKYYRENHPAVLKLIEHVIKTCKKKGVKTSICGQAGSRP
AIVEKLVEWGITSISSNIDAIDTIRQTVARTEQRIILDIIRDSKNRSGL

Sequences:

>Translated_769_residues
MTLIAWLDDLSNEDVNIAGGKGASLGEMWNAGLPVPPAFVVTAEAYRYFINETKLNNKIMEVLEGLDVNNNDELTKKSAE
VRKLIESAEMPTDLKIAIVESYSKLGQMTGTDETFVAVRSSATAEDLPDASFAGQQDTYLNMKGADDVVDAVQRCFASLF
TPRAVFYREQKGFDHFEVALAAVVQKMVNADKAGVMFTVNPINHNYDEIVIEGAWGLGEGVVSGTVSPDTYIVSKDKKIL
DVYIATKETMFEKDENGITREIPTPDELKDKRVLSDEELLKLVDKGLVIEKHYNRPMDIEWALENEGVYMLQARPITTLD
EKPAKTGGGAKPPSSEDAGGNIILKGIGASPGTASGTVKILNKIEEIDKIKEGDILVTKMTTPDMVPAMKKASAIVTNDG
GLTCHAAIISRELGTPCVVGTKKATDVLKEGTIITIDGEKGVVYEGELAKKEEAVQARVVYQGAPLITATEIKVNVSMPE
VAERAAATNADGVGLLRAEHMILGTGVHPLKVLKDKGEEGLINVFADGIRVVADAFYPRTITYRTLDAPTDEFRGLEGGE
DEPEEHNPMLGWRGIRRGLDQPEILRCELLAVKKLLNEGYKNIMVMIPLVSSVDEIKKVRKLADTVGLRLGKDVEFGVMV
ETPAAALIIEDIVETDIDFVSLGTNDLTQYTIAIDRNNEYVAKYYRENHPAVLKLIEHVIKTCKKKGVKTSICGQAGSRP
AIVEKLVEWGITSISSNIDAIDTIRQTVARTEQRIILDIIRDSKNRSGL
>Mature_768_residues
TLIAWLDDLSNEDVNIAGGKGASLGEMWNAGLPVPPAFVVTAEAYRYFINETKLNNKIMEVLEGLDVNNNDELTKKSAEV
RKLIESAEMPTDLKIAIVESYSKLGQMTGTDETFVAVRSSATAEDLPDASFAGQQDTYLNMKGADDVVDAVQRCFASLFT
PRAVFYREQKGFDHFEVALAAVVQKMVNADKAGVMFTVNPINHNYDEIVIEGAWGLGEGVVSGTVSPDTYIVSKDKKILD
VYIATKETMFEKDENGITREIPTPDELKDKRVLSDEELLKLVDKGLVIEKHYNRPMDIEWALENEGVYMLQARPITTLDE
KPAKTGGGAKPPSSEDAGGNIILKGIGASPGTASGTVKILNKIEEIDKIKEGDILVTKMTTPDMVPAMKKASAIVTNDGG
LTCHAAIISRELGTPCVVGTKKATDVLKEGTIITIDGEKGVVYEGELAKKEEAVQARVVYQGAPLITATEIKVNVSMPEV
AERAAATNADGVGLLRAEHMILGTGVHPLKVLKDKGEEGLINVFADGIRVVADAFYPRTITYRTLDAPTDEFRGLEGGED
EPEEHNPMLGWRGIRRGLDQPEILRCELLAVKKLLNEGYKNIMVMIPLVSSVDEIKKVRKLADTVGLRLGKDVEFGVMVE
TPAAALIIEDIVETDIDFVSLGTNDLTQYTIAIDRNNEYVAKYYRENHPAVLKLIEHVIKTCKKKGVKTSICGQAGSRPA
IVEKLVEWGITSISSNIDAIDTIRQTVARTEQRIILDIIRDSKNRSGL

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=800, Percent_Identity=38.125, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1788756, Length=420, Percent_Identity=32.3809523809524, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1789193, Length=371, Percent_Identity=31.266846361186, Blast_Score=162, Evalue=9e-41,
Organism=Escherichia coli, GI48994992, Length=379, Percent_Identity=28.4960422163588, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788726, Length=379, Percent_Identity=26.1213720316623, Blast_Score=119, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17564524, Length=326, Percent_Identity=29.1411042944785, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 83993; Mature: 83862

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIAWLDDLSNEDVNIAGGKGASLGEMWNAGLPVPPAFVVTAEAYRYFINETKLNNKIM
CEEEEEECCCCCCCEEEECCCCCCHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
EVLEGLDVNNNDELTKKSAEVRKLIESAEMPTDLKIAIVESYSKLGQMTGTDETFVAVRS
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCCEEEEEEC
SATAEDLPDASFAGQQDTYLNMKGADDVVDAVQRCFASLFTPRAVFYREQKGFDHFEVAL
CCCHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCHHHEEHHHCCCCHHHHHH
AAVVQKMVNADKAGVMFTVNPINHNYDEIVIEGAWGLGEGVVSGTVSPDTYIVSKDKKIL
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCEECCCCCCEEEEECCCEEE
DVYIATKETMFEKDENGITREIPTPDELKDKRVLSDEELLKLVDKGLVIEKHYNRPMDIE
EEEEECHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCEE
WALENEGVYMLQARPITTLDEKPAKTGGGAKPPSSEDAGGNIILKGIGASPGTASGTVKI
EEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHH
LNKIEEIDKIKEGDILVTKMTTPDMVPAMKKASAIVTNDGGLTCHAAIISRELGTPCVVG
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCEEEHHHHHHHCCCCEEEC
TKKATDVLKEGTIITIDGEKGVVYEGELAKKEEAVQARVVYQGAPLITATEIKVNVSMPE
CHHHHHHHHCCCEEEEECCCCEEECCCHHHHHHHHEEEEEECCCCEEEEEEEEEECCCHH
VAERAAATNADGVGLLRAEHMILGTGVHPLKVLKDKGEEGLINVFADGIRVVADAFYPRT
HHHHHHCCCCCCCEEEEECEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCE
ITYRTLDAPTDEFRGLEGGEDEPEEHNPMLGWRGIRRGLDQPEILRCELLAVKKLLNEGY
EEEEECCCCHHHHCCCCCCCCCCHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
KNIMVMIPLVSSVDEIKKVRKLADTVGLRLGKDVEFGVMVETPAAALIIEDIVETDIDFV
HEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEE
SLGTNDLTQYTIAIDRNNEYVAKYYRENHPAVLKLIEHVIKTCKKKGVKTSICGQAGSRP
ECCCCCCEEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCH
AIVEKLVEWGITSISSNIDAIDTIRQTVARTEQRIILDIIRDSKNRSGL
HHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TLIAWLDDLSNEDVNIAGGKGASLGEMWNAGLPVPPAFVVTAEAYRYFINETKLNNKIM
EEEEEECCCCCCCEEEECCCCCCHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
EVLEGLDVNNNDELTKKSAEVRKLIESAEMPTDLKIAIVESYSKLGQMTGTDETFVAVRS
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCCEEEEEEC
SATAEDLPDASFAGQQDTYLNMKGADDVVDAVQRCFASLFTPRAVFYREQKGFDHFEVAL
CCCHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCHHHEEHHHCCCCHHHHHH
AAVVQKMVNADKAGVMFTVNPINHNYDEIVIEGAWGLGEGVVSGTVSPDTYIVSKDKKIL
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCEECCCCCCEEEEECCCEEE
DVYIATKETMFEKDENGITREIPTPDELKDKRVLSDEELLKLVDKGLVIEKHYNRPMDIE
EEEEECHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCEE
WALENEGVYMLQARPITTLDEKPAKTGGGAKPPSSEDAGGNIILKGIGASPGTASGTVKI
EEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHH
LNKIEEIDKIKEGDILVTKMTTPDMVPAMKKASAIVTNDGGLTCHAAIISRELGTPCVVG
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCEEEHHHHHHHCCCCEEEC
TKKATDVLKEGTIITIDGEKGVVYEGELAKKEEAVQARVVYQGAPLITATEIKVNVSMPE
CHHHHHHHHCCCEEEEECCCCEEECCCHHHHHHHHEEEEEECCCCEEEEEEEEEECCCHH
VAERAAATNADGVGLLRAEHMILGTGVHPLKVLKDKGEEGLINVFADGIRVVADAFYPRT
HHHHHHCCCCCCCEEEEECEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCE
ITYRTLDAPTDEFRGLEGGEDEPEEHNPMLGWRGIRRGLDQPEILRCELLAVKKLLNEGY
EEEEECCCCHHHHCCCCCCCCCCHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
KNIMVMIPLVSSVDEIKKVRKLADTVGLRLGKDVEFGVMVETPAAALIIEDIVETDIDFV
HEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEE
SLGTNDLTQYTIAIDRNNEYVAKYYRENHPAVLKLIEHVIKTCKKKGVKTSICGQAGSRP
ECCCCCCEEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCH
AIVEKLVEWGITSISSNIDAIDTIRQTVARTEQRIILDIIRDSKNRSGL
HHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]