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The map label for this gene is infC

Identifier: 150398669

GI number: 150398669

Start: 3690912

End: 3691310

Strand: Direct

Name: infC

Synonym: Smed_3483

Alternate gene names: 150398669

Gene position: 3690912-3691310 (Clockwise)

Preceding gene: 150398666

Following gene: 150398670

Centisome position: 97.59

GC content: 56.64

Gene sequence:

>399_bases
ATGGCGGACGAGGCCGGTCTCGATCTGGTGGAGATTGCACCGAACTCCGATCCGCCGGTGTGCAAGATTCTCGACCTCGG
CAAGCTGAAATACGCGAACCAGAAGAAAGCCGCCGAGGCGCGCAAGAAGCAGAAGATCGTCGAGATCAAGGAAATCAAGA
TGCGCCCCAACATCGACACCCATGATTATGAGGTGAAGATGAAGGCCATGAACCGCTTCTTCGAGGAAGGCGACAAGGTC
AAGGTGACGCTGAAGTTCCGCGGCCGCGAAATGGCCCACCAGGAACTCGGCATGAAACTTCTGATGCAGGTTAAGGACGA
CACCCAGGAAATCGCCAAGGTGGAAGCCGAACCCAAGCTCGAAGGCCGCCAGATGATGATGGTGCTTGCGCCGAAGTGA

Upstream 100 bases:

>100_bases
AGGACCGCGCGCAAACAAGGAAATCCGGGTCCCCCGGGTTCAGCTTATCGATGCCGAAGGCCAGAACTCGGGCATCGTTC
CGATCGATGAGGCGCTCCGC

Downstream 100 bases:

>100_bases
TCGCGGAACCTGATCGGACAGGATGCGCTTAGGGAAAGCCGCCCCGGGGGAGGAGTCCGTGGGGCGGCTTTCTTTTTTGG
GAGGTCTTCTCCCCATTTAT

Product: translation initiation factor IF-3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MADEAGLDLVEIAPNSDPPVCKILDLGKLKYANQKKAAEARKKQKIVEIKEIKMRPNIDTHDYEVKMKAMNRFFEEGDKV
KVTLKFRGREMAHQELGMKLLMQVKDDTQEIAKVEAEPKLEGRQMMMVLAPK

Sequences:

>Translated_132_residues
MADEAGLDLVEIAPNSDPPVCKILDLGKLKYANQKKAAEARKKQKIVEIKEIKMRPNIDTHDYEVKMKAMNRFFEEGDKV
KVTLKFRGREMAHQELGMKLLMQVKDDTQEIAKVEAEPKLEGRQMMMVLAPK
>Mature_131_residues
ADEAGLDLVEIAPNSDPPVCKILDLGKLKYANQKKAAEARKKQKIVEIKEIKMRPNIDTHDYEVKMKAMNRFFEEGDKVK
VTLKFRGREMAHQELGMKLLMQVKDDTQEIAKVEAEPKLEGRQMMMVLAPK

Specific function: IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins

COG id: COG0290

COG function: function code J; Translation initiation factor 3 (IF-3)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-3 family

Homologues:

Organism=Escherichia coli, GI1788012, Length=132, Percent_Identity=61.3636363636364, Blast_Score=164, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IF3_RHIME (Q92ST3)

Other databases:

- EMBL:   AL591688
- RefSeq:   NP_384388.1
- ProteinModelPortal:   Q92ST3
- GeneID:   1231916
- GenomeReviews:   AL591688_GR
- KEGG:   sme:SMc00362
- NMPDR:   fig|266834.1.peg.1576
- HOGENOM:   HBG744268
- ProtClustDB:   PRK00028
- BioCyc:   SMEL266834:SMC00362-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00080
- InterPro:   IPR001288
- InterPro:   IPR019815
- InterPro:   IPR019814
- Gene3D:   G3DSA:3.10.20.80
- Gene3D:   G3DSA:3.30.110.10
- PANTHER:   PTHR10938
- TIGRFAMs:   TIGR00168

Pfam domain/function: PF00707 IF3_C; PF05198 IF3_N; SSF54364 IF3; SSF55200 IF3

EC number: NA

Molecular weight: Translated: 15142; Mature: 15011

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00938 IF3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
7.6 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
6.9 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEAGLDLVEIAPNSDPPVCKILDLGKLKYANQKKAAEARKKQKIVEIKEIKMRPNIDT
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCC
HDYEVKMKAMNRFFEEGDKVKVTLKFRGREMAHQELGMKLLMQVKDDTQEIAKVEAEPKL
CCCEEHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
EGRQMMMVLAPK
CCCEEEEEEECC
>Mature Secondary Structure 
ADEAGLDLVEIAPNSDPPVCKILDLGKLKYANQKKAAEARKKQKIVEIKEIKMRPNIDT
CCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCC
HDYEVKMKAMNRFFEEGDKVKVTLKFRGREMAHQELGMKLLMQVKDDTQEIAKVEAEPKL
CCCEEHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
EGRQMMMVLAPK
CCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430