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The map label for this gene is recF [H]

Identifier: 150398584

GI number: 150398584

Start: 3600571

End: 3601800

Strand: Direct

Name: recF [H]

Synonym: Smed_3395

Alternate gene names: 150398584

Gene position: 3600571-3601800 (Clockwise)

Preceding gene: 150398579

Following gene: 150398585

Centisome position: 95.21

GC content: 66.34

Gene sequence:

>1230_bases
GTGCGATCCGCTACACGCACAAGGGCGGTCAGCGCCGGATGCCCTTCCGCTTGCCTTGAGGCTGCTTTCGTCCTACCTGA
CAGCAACGTCGAATTCGGACCCGCAATGCCCCACAAGGTCTTTCTCACACGCCTGAAACTGAGCGATTTCCGCAACTATG
CAACGGCGGCGCTCGACCTCGACCAGCGCCATGTGGTGCTGACCGGCGAGAACGGCGCTGGCAAGACCAACCTGATGGAG
GCGATCTCTTTTCTGTCTCCCGGGCGAGGCTTGAGGCGCGCTGCGTATGCCGATGTCGTCCGCGTCGGCGCCGCTGACGG
CTTCTCGGTCTTCGCCGCTGTCGATGGGATGGAGGGGCCGGTGGAGATCGGCACGGGGACTCAGGGATCCGAGGAGGGTC
ATTCGCGCCGCCTCAGAATCAACGGCACTGCGGCGCGCACCGTCGACGAACTCAACGACCACCTCCGGGTGCTCTGGCTG
ACCCCCGCGATGGATGGCCTTTTTACCGGCCCTTCCGCCGACCGCAGGCGCTTTCTCGACCGGCTGGTGCTTTCGCTCGA
TCCCGAGCACGGCCGCCGCGCCAGCGAGTTCGACCGCGCCATGCGCAGCCGCAACAGGCTTCTTTCGGAGTTCCGCCCCG
ATCCCGCCTGGGTATCGGCCATCGAACGCGAAATGGCCGGCCTCGGCATTTCCATGGCGCTTGCCCGCCAGGAGATGCTC
GGCCTGCTTTCCGCTCTCGTCGACCGTAGCAGGACCGACGGGACCTTTCCGTCCGCACGTCTTTCGCTTGCCGGCTTCCT
CGATGACTGCGCCGGTATTCCGGCCTTTGAACTCGAAGAGCGCTATCTCGCAATGCTCGCTGATGGCCGGGCGCGCGATG
CCGCCGCCGGCCGCACGCTCGACGGGCCGCACCGCAGTGATCTCCTGATCCGCCACCGGGAAAAGGACATCGAAGCCGAG
CGTTGCTCGACCGGCGAACAGAAGGCGCTGCTCGTCGGCCTGGTGCTCGCGCATGCACGCCTCGTCGGCGACATGACGGG
CCACGCCCCGGTGCTGCTGCTCGACGAAATCGCCGCCCATCTCGACCAGGGACGCCGTGCCGCCCTCTTCGACCTCGTCG
ATGGGCTCGGCGGCCAGGCCTTCATGACGGGCACCGACCAAACCATGTTCGAGGCGCTCGGGGAGCGCGCGCATTATCTG
GCCGTTGCAAACGGCCGCGTTTCAGGATGA

Upstream 100 bases:

>100_bases
ATTGTCACGCAGTTGTCATATTGTACTTGCACGCTCGGGGAAAGTTCCGATGCGGTCGAAAATAACGGCTTTCCCACAGC
GGATTGGGCGCAAACGGGCC

Downstream 100 bases:

>100_bases
CATGGAGCGAGCGCCGTCCTCACAGGATAACGAAATGAGATGGAGACGCATATGACGACGGGTGCGACACTCGCTCCTTC
GGAGATTGCGCGCTATGCAC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MRSATRTRAVSAGCPSACLEAAFVLPDSNVEFGPAMPHKVFLTRLKLSDFRNYATAALDLDQRHVVLTGENGAGKTNLME
AISFLSPGRGLRRAAYADVVRVGAADGFSVFAAVDGMEGPVEIGTGTQGSEEGHSRRLRINGTAARTVDELNDHLRVLWL
TPAMDGLFTGPSADRRRFLDRLVLSLDPEHGRRASEFDRAMRSRNRLLSEFRPDPAWVSAIEREMAGLGISMALARQEML
GLLSALVDRSRTDGTFPSARLSLAGFLDDCAGIPAFELEERYLAMLADGRARDAAAGRTLDGPHRSDLLIRHREKDIEAE
RCSTGEQKALLVGLVLAHARLVGDMTGHAPVLLLDEIAAHLDQGRRAALFDLVDGLGGQAFMTGTDQTMFEALGERAHYL
AVANGRVSG

Sequences:

>Translated_409_residues
MRSATRTRAVSAGCPSACLEAAFVLPDSNVEFGPAMPHKVFLTRLKLSDFRNYATAALDLDQRHVVLTGENGAGKTNLME
AISFLSPGRGLRRAAYADVVRVGAADGFSVFAAVDGMEGPVEIGTGTQGSEEGHSRRLRINGTAARTVDELNDHLRVLWL
TPAMDGLFTGPSADRRRFLDRLVLSLDPEHGRRASEFDRAMRSRNRLLSEFRPDPAWVSAIEREMAGLGISMALARQEML
GLLSALVDRSRTDGTFPSARLSLAGFLDDCAGIPAFELEERYLAMLADGRARDAAAGRTLDGPHRSDLLIRHREKDIEAE
RCSTGEQKALLVGLVLAHARLVGDMTGHAPVLLLDEIAAHLDQGRRAALFDLVDGLGGQAFMTGTDQTMFEALGERAHYL
AVANGRVSG
>Mature_409_residues
MRSATRTRAVSAGCPSACLEAAFVLPDSNVEFGPAMPHKVFLTRLKLSDFRNYATAALDLDQRHVVLTGENGAGKTNLME
AISFLSPGRGLRRAAYADVVRVGAADGFSVFAAVDGMEGPVEIGTGTQGSEEGHSRRLRINGTAARTVDELNDHLRVLWL
TPAMDGLFTGPSADRRRFLDRLVLSLDPEHGRRASEFDRAMRSRNRLLSEFRPDPAWVSAIEREMAGLGISMALARQEML
GLLSALVDRSRTDGTFPSARLSLAGFLDDCAGIPAFELEERYLAMLADGRARDAAAGRTLDGPHRSDLLIRHREKDIEAE
RCSTGEQKALLVGLVLAHARLVGDMTGHAPVLLLDEIAAHLDQGRRAALFDLVDGLGGQAFMTGTDQTMFEALGERAHYL
AVANGRVSG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=371, Percent_Identity=28.0323450134771, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 44218; Mature: 44218

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSATRTRAVSAGCPSACLEAAFVLPDSNVEFGPAMPHKVFLTRLKLSDFRNYATAALDL
CCCCHHHHHHCCCCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEC
DQRHVVLTGENGAGKTNLMEAISFLSPGRGLRRAAYADVVRVGAADGFSVFAAVDGMEGP
CCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
VEIGTGTQGSEEGHSRRLRINGTAARTVDELNDHLRVLWLTPAMDGLFTGPSADRRRFLD
EEECCCCCCCCCCCCCEEEECCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHH
RLVLSLDPEHGRRASEFDRAMRSRNRLLSEFRPDPAWVSAIEREMAGLGISMALARQEML
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
GLLSALVDRSRTDGTFPSARLSLAGFLDDCAGIPAFELEERYLAMLADGRARDAAAGRTL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCCCC
DGPHRSDLLIRHREKDIEAERCSTGEQKALLVGLVLAHARLVGDMTGHAPVLLLDEIAAH
CCCCCCCHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LDQGRRAALFDLVDGLGGQAFMTGTDQTMFEALGERAHYLAVANGRVSG
HCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MRSATRTRAVSAGCPSACLEAAFVLPDSNVEFGPAMPHKVFLTRLKLSDFRNYATAALDL
CCCCHHHHHHCCCCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEC
DQRHVVLTGENGAGKTNLMEAISFLSPGRGLRRAAYADVVRVGAADGFSVFAAVDGMEGP
CCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
VEIGTGTQGSEEGHSRRLRINGTAARTVDELNDHLRVLWLTPAMDGLFTGPSADRRRFLD
EEECCCCCCCCCCCCCEEEECCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHH
RLVLSLDPEHGRRASEFDRAMRSRNRLLSEFRPDPAWVSAIEREMAGLGISMALARQEML
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
GLLSALVDRSRTDGTFPSARLSLAGFLDDCAGIPAFELEERYLAMLADGRARDAAAGRTL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCCCC
DGPHRSDLLIRHREKDIEAERCSTGEQKALLVGLVLAHARLVGDMTGHAPVLLLDEIAAH
CCCCCCCHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LDQGRRAALFDLVDGLGGQAFMTGTDQTMFEALGERAHYLAVANGRVSG
HCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9696772 [H]