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The map label for this gene is gspE [C]

Identifier: 150398544

GI number: 150398544

Start: 3554032

End: 3555498

Strand: Direct

Name: gspE [C]

Synonym: Smed_3355

Alternate gene names: 150398544

Gene position: 3554032-3555498 (Clockwise)

Preceding gene: 150398543

Following gene: 150398545

Centisome position: 93.97

GC content: 63.46

Gene sequence:

>1467_bases
ATGTTTGGCAAACGCGGAAATGAAGGTCCTGGCAAGGGTGGTGCACGCGGTTTCACACCTGCGCCTTCAATACCCGCGGT
CGAGCTGGCGGTTGTCGAGCGTCCCTCGGCCCCTGATTATGGAGAGCCGGCCGCTCCGCCATCCCGGCCGCAGGCGGCAG
CGCCCCCTCAGCGCCGGCGCCCGGCGCGGGCAGAGGATTACTACGACACGAAATCGCAGGTCTTCTCCGCGCTGATCGAC
ACGATCGACCTGTCGCAGCTCTCCAAGCTCGATATCGAGAGCGCGCGCGAGGAAATTCGCGACATCGTCAACGACATCAT
CACCATCAAGAATTTCGCGATGTCGATCTCGGAGCAGGAGGAACTGCTCGACGACATCTGCAACGACGTGCTCGGCTACG
GACCGCTGGAGCCGCTGCTCGCGCGCGACGATATCGCCGACATCATGGTCAACGGCGCCGGGCAAACCTTCATCGAAGTG
GGCGGGAAGGTCGAAGAATCGGAGATACGGTTCCGCGACAACGGGCAACTCCTGTCGATCTGCCAGCGCATCGTCAGTCA
GGTGGGCCGCCGCGTAGACGAGTCCAGCCCGATCTGCGACGCGCGTCTGCCGGATGGCTCGCGTGTCAACGTCATCGCCC
CGCCGCTCGCGATCGACGGCACGGCACTCACGATCCGCAAGTTCAAAAAGGACAAGCTGACGCTCGAGCAGCTGGTACGT
TTCGGCTCGGTCACACCGGAGGCCGCGGTGCTGCTGCAGATCATCGGCCGCGTCCGCTGCAACATCGTCATCTCCGGCGG
TACCGGCTCCGGCAAGACGACGCTGCTCAACTGCCTGACGCGCTATATAGACAGCAACGAGCGCATCATCACCTGCGAAG
ACTCCGCCGAACTGCAATTGCAGCAGCCGCATGTGGTCCGTCTCGAGACTCGCCCGCCGAACATCGAGGGCGAGGGCGAG
ATCACCATGCGCGACCTGGTGAAGAACTGCCTGCGCATGCGCCCGGAGCGCATCATCGTCGGCGAGGTGCGCGGCCCGGA
AGTCTTCGATCTGCTGCAGGCGATGAACACCGGCCATGACGGATCGATGGGAACCATCCACGCGAACACGCCGCGCGAAT
GCCTGAGCCGAATGGAATCGATGATCGCCATGGGCGGTTACACCCTGCCTGCCAGGACCGTGCGCGAAATCATCTCCGGC
TCGGTGGACGTCATCATCCAGGCATCGCGCCTGCGCGACGGTTCGCGCCGGATCACCCACATCACCGAGGTCGTCGGCAT
GGAAGGCGACGTAATCATCACACAGGATCTGATGCGCTACGAGATCGACGGCGAGGATGCCAATGGCCGGATTGTCGGCC
GCCACGTTTCGACCGGGATAGGCCGGCCGCATTTCTGGGACCGGGCCCGCTACTTCAACGAGGACAAGCGGCTCGCCGCG
ACACTCGATGCGATGGAAAAGCAATAG

Upstream 100 bases:

>100_bases
GGCCTCGGCAAGGTTCTGGCAAAGCTCGGAAAGCGGTAGCGGCAATATTATCGATCCTGCATCCGCAGGTAACGAGTGCT
GAAGCAATTGGAATGAAGTC

Downstream 100 bases:

>100_bases
AGCGGGATGAGGCCGCTGCTCTAGCGCAGCATACTTCTGACGCGAGGGGACAGACCGAGCGATGTTCGGCATAGACATCA
CCGTCCTGGGATTGGCCGGC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MFGKRGNEGPGKGGARGFTPAPSIPAVELAVVERPSAPDYGEPAAPPSRPQAAAPPQRRRPARAEDYYDTKSQVFSALID
TIDLSQLSKLDIESAREEIRDIVNDIITIKNFAMSISEQEELLDDICNDVLGYGPLEPLLARDDIADIMVNGAGQTFIEV
GGKVEESEIRFRDNGQLLSICQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKFKKDKLTLEQLVR
FGSVTPEAAVLLQIIGRVRCNIVISGGTGSGKTTLLNCLTRYIDSNERIITCEDSAELQLQQPHVVRLETRPPNIEGEGE
ITMRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHDGSMGTIHANTPRECLSRMESMIAMGGYTLPARTVREIISG
SVDVIIQASRLRDGSRRITHITEVVGMEGDVIITQDLMRYEIDGEDANGRIVGRHVSTGIGRPHFWDRARYFNEDKRLAA
TLDAMEKQ

Sequences:

>Translated_488_residues
MFGKRGNEGPGKGGARGFTPAPSIPAVELAVVERPSAPDYGEPAAPPSRPQAAAPPQRRRPARAEDYYDTKSQVFSALID
TIDLSQLSKLDIESAREEIRDIVNDIITIKNFAMSISEQEELLDDICNDVLGYGPLEPLLARDDIADIMVNGAGQTFIEV
GGKVEESEIRFRDNGQLLSICQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKFKKDKLTLEQLVR
FGSVTPEAAVLLQIIGRVRCNIVISGGTGSGKTTLLNCLTRYIDSNERIITCEDSAELQLQQPHVVRLETRPPNIEGEGE
ITMRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHDGSMGTIHANTPRECLSRMESMIAMGGYTLPARTVREIISG
SVDVIIQASRLRDGSRRITHITEVVGMEGDVIITQDLMRYEIDGEDANGRIVGRHVSTGIGRPHFWDRARYFNEDKRLAA
TLDAMEKQ
>Mature_488_residues
MFGKRGNEGPGKGGARGFTPAPSIPAVELAVVERPSAPDYGEPAAPPSRPQAAAPPQRRRPARAEDYYDTKSQVFSALID
TIDLSQLSKLDIESAREEIRDIVNDIITIKNFAMSISEQEELLDDICNDVLGYGPLEPLLARDDIADIMVNGAGQTFIEV
GGKVEESEIRFRDNGQLLSICQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKFKKDKLTLEQLVR
FGSVTPEAAVLLQIIGRVRCNIVISGGTGSGKTTLLNCLTRYIDSNERIITCEDSAELQLQQPHVVRLETRPPNIEGEGE
ITMRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHDGSMGTIHANTPRECLSRMESMIAMGGYTLPARTVREIISG
SVDVIIQASRLRDGSRRITHITEVVGMEGDVIITQDLMRYEIDGEDANGRIVGRHVSTGIGRPHFWDRARYFNEDKRLAA
TLDAMEKQ

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 53721; Mature: 53721

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKRGNEGPGKGGARGFTPAPSIPAVELAVVERPSAPDYGEPAAPPSRPQAAAPPQRRR
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCC
PARAEDYYDTKSQVFSALIDTIDLSQLSKLDIESAREEIRDIVNDIITIKNFAMSISEQE
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLDDICNDVLGYGPLEPLLARDDIADIMVNGAGQTFIEVGGKVEESEIRFRDNGQLLSI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCEECCCEEECCCCHHHHH
CQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKFKKDKLTLEQLVR
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEECCCEEEHHHHHHHHHHHHHHHH
FGSVTPEAAVLLQIIGRVRCNIVISGGTGSGKTTLLNCLTRYIDSNERIITCEDSAELQL
HCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEE
QQPHVVRLETRPPNIEGEGEITMRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHD
CCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCC
GSMGTIHANTPRECLSRMESMIAMGGYTLPARTVREIISGSVDVIIQASRLRDGSRRITH
CCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEHHHCCCCCHHHHH
ITEVVGMEGDVIITQDLMRYEIDGEDANGRIVGRHVSTGIGRPHFWDRARYFNEDKRLAA
HHHHHCCCCCEEEECCHHHEEECCCCCCCEEEEEEHHCCCCCCCHHHHHHHHCCCHHHHH
TLDAMEKQ
HHHHHHCC
>Mature Secondary Structure
MFGKRGNEGPGKGGARGFTPAPSIPAVELAVVERPSAPDYGEPAAPPSRPQAAAPPQRRR
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCC
PARAEDYYDTKSQVFSALIDTIDLSQLSKLDIESAREEIRDIVNDIITIKNFAMSISEQE
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLDDICNDVLGYGPLEPLLARDDIADIMVNGAGQTFIEVGGKVEESEIRFRDNGQLLSI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCEECCCEEECCCCHHHHH
CQRIVSQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGTALTIRKFKKDKLTLEQLVR
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEECCCEEEHHHHHHHHHHHHHHHH
FGSVTPEAAVLLQIIGRVRCNIVISGGTGSGKTTLLNCLTRYIDSNERIITCEDSAELQL
HCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEE
QQPHVVRLETRPPNIEGEGEITMRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHD
CCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCC
GSMGTIHANTPRECLSRMESMIAMGGYTLPARTVREIISGSVDVIIQASRLRDGSRRITH
CCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEHHHCCCCCHHHHH
ITEVVGMEGDVIITQDLMRYEIDGEDANGRIVGRHVSTGIGRPHFWDRARYFNEDKRLAA
HHHHHCCCCCEEEECCHHHEEECCCCCCCEEEEEEHHCCCCCCCHHHHHHHHCCCHHHHH
TLDAMEKQ
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]