Click here to switch to the map view.

The map label for this gene is lutR [H]

Identifier: 150398510

GI number: 150398510

Start: 3518696

End: 3519418

Strand: Direct

Name: lutR [H]

Synonym: Smed_3321

Alternate gene names: 150398510

Gene position: 3518696-3519418 (Clockwise)

Preceding gene: 150398507

Following gene: 150398511

Centisome position: 93.04

GC content: 63.07

Gene sequence:

>723_bases
TTGAACGACGTAACATTGAAACTGAAACCGGCGCGGCGGGAGAGGCTCGCAGACGTTCTCTACGGACAGATCCTCGAACA
GATCGTAAGCGGCCAGATGGCCGAAGGCGCGAAACTGCCGTCGGAAGCGCATATCTGCCGCGACTTTCAAGTTTCCCGTC
CGGTCGTGCGAGAGGCGCTGATGCGGTTGCAGGCCGACGGGCTGGTCGTGTCGCGCCAGGGGATCGGCACCTTCGTCAAA
TCGCGTCCGTCCGACAAGCTCACCAGTCTCGCCGCCTCGGCCGAGATCGCGAGCTATCTCAGGGCTTTCGAGCCCCGGCT
CGCGCTCGAGACGGAGTGTGCGGGACTTGCCGCGATGCGCCGTACAAAGGCCCAGCTCAACCAGATAGGCGAGGCGCTGT
CGGCCTTGGAAGGCGCTTTTGCGCGCGGCGAAACCGGATGGGAAGAGGACTTCGCCTTTCACCACGCCGTGGCTGAGGCT
GCGGGAAACGAACTTTTTCCGCGACTTCTGGAGGATTTGCGTGAAATTCTTAGCGGCTCGATGCGCATGGCGCTCGGTCT
GACGCGGCAGGGCTCCGATGACCGCCGCCGCCGTGTCCGGGAAGAGCACCGCAAGATCTACTCTGCCATTTCCGGCCAGA
ACCGGGATCTCGCCCGCCTTTACATGCGCTACCATCTGACGGAATCGCGCGCCCGTATCACCGGGTCCCATGGCGATCTC
TGA

Upstream 100 bases:

>100_bases
TCCGGATTCTTGCGTCTGCTCATTGGCGGCCTCCTTTGCATTTCCAGGCGGGTCGGCTAGATTGCGCCTGTACAGAAAAA
TGACAGGTGAAGGCAGACCC

Downstream 100 bases:

>100_bases
TCCGGTCATGCTTCGACCCATTTTACGCGCTCACCGATAGCTCGATTCGACCATGTCGGACGGCGTTTTCATGCAACCCT
CCCAGCCGATTTCCTGTATA

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MNDVTLKLKPARRERLADVLYGQILEQIVSGQMAEGAKLPSEAHICRDFQVSRPVVREALMRLQADGLVVSRQGIGTFVK
SRPSDKLTSLAASAEIASYLRAFEPRLALETECAGLAAMRRTKAQLNQIGEALSALEGAFARGETGWEEDFAFHHAVAEA
AGNELFPRLLEDLREILSGSMRMALGLTRQGSDDRRRRVREEHRKIYSAISGQNRDLARLYMRYHLTESRARITGSHGDL

Sequences:

>Translated_240_residues
MNDVTLKLKPARRERLADVLYGQILEQIVSGQMAEGAKLPSEAHICRDFQVSRPVVREALMRLQADGLVVSRQGIGTFVK
SRPSDKLTSLAASAEIASYLRAFEPRLALETECAGLAAMRRTKAQLNQIGEALSALEGAFARGETGWEEDFAFHHAVAEA
AGNELFPRLLEDLREILSGSMRMALGLTRQGSDDRRRRVREEHRKIYSAISGQNRDLARLYMRYHLTESRARITGSHGDL
>Mature_240_residues
MNDVTLKLKPARRERLADVLYGQILEQIVSGQMAEGAKLPSEAHICRDFQVSRPVVREALMRLQADGLVVSRQGIGTFVK
SRPSDKLTSLAASAEIASYLRAFEPRLALETECAGLAAMRRTKAQLNQIGEALSALEGAFARGETGWEEDFAFHHAVAEA
AGNELFPRLLEDLREILSGSMRMALGLTRQGSDDRRRRVREEHRKIYSAISGQNRDLARLYMRYHLTESRARITGSHGDL

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=182, Percent_Identity=36.2637362637363, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48994955, Length=202, Percent_Identity=28.2178217821782, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790780, Length=212, Percent_Identity=28.7735849056604, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1790032, Length=230, Percent_Identity=27.3913043478261, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26776; Mature: 26776

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVTLKLKPARRERLADVLYGQILEQIVSGQMAEGAKLPSEAHICRDFQVSRPVVREAL
CCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCHHHHHHH
MRLQADGLVVSRQGIGTFVKSRPSDKLTSLAASAEIASYLRAFEPRLALETECAGLAAMR
HHHHHCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RTKAQLNQIGEALSALEGAFARGETGWEEDFAFHHAVAEAAGNELFPRLLEDLREILSGS
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCH
MRMALGLTRQGSDDRRRRVREEHRKIYSAISGQNRDLARLYMRYHLTESRARITGSHGDL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNDVTLKLKPARRERLADVLYGQILEQIVSGQMAEGAKLPSEAHICRDFQVSRPVVREAL
CCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCHHHHHHH
MRLQADGLVVSRQGIGTFVKSRPSDKLTSLAASAEIASYLRAFEPRLALETECAGLAAMR
HHHHHCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RTKAQLNQIGEALSALEGAFARGETGWEEDFAFHHAVAEAAGNELFPRLLEDLREILSGS
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCH
MRMALGLTRQGSDDRRRRVREEHRKIYSAISGQNRDLARLYMRYHLTESRARITGSHGDL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]