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The map label for this gene is hslU [H]

Identifier: 150398458

GI number: 150398458

Start: 3444191

End: 3445498

Strand: Direct

Name: hslU [H]

Synonym: Smed_3266

Alternate gene names: 150398458

Gene position: 3444191-3445498 (Clockwise)

Preceding gene: 150398457

Following gene: 150398459

Centisome position: 91.07

GC content: 62.69

Gene sequence:

>1308_bases
ATGAGCAGTTTTTCACCCAGGGAAATCGTATCGGAACTCGACCGCTACATTATCGGCCAGAAGGACGCGAAGCGCGCCGT
TGCCATTGCGCTGCGCAACCGATGGCGCCGCCAGCAGCTCAGCGACGAACTGCGCGACGAGGTCATGCCCAAGAATATCT
TGATGATCGGCCCGACCGGCGTCGGCAAAACGGAAATTTCCCGCCGCCTCGCCAAGCTCGCCGGCGCGCCTTTCGTCAAG
GTGGAGGCCACGAAGTTCACGGAAGTCGGCTATGTCGGCCGCGATGTCGAACAGATCGTGCGCGATCTCGTCGAGGTCGG
CATCAGCCTCGTGCGCGAGAAGCGGCGCGCCGAAGTCAAGGCGAAAGCTCATCAGAATGCCGAGGAGCGGGTTCTCGATG
CTCTGGTCGGCACCACCGCCTCGCCGGCGACCCGCGACTCCTTCCGCAAGAAACTGCGCGCCAACGAGCTCGACGACAAG
GAGATCGAGGTCGATGTCGCGGAAACCGGCAACCCCGGCGGCGCCTTCGAGATTCCGGGGATGCCGGGCGCCAATATCGG
CGTCCTGAACCTCTCGGAAATGTTCGGCAAGGCGCTGGGTGGCAGGACCAGGAAGATCAAGACCACGGTCAAGGACTCCT
ATGCGCTGCTGGTCAACGACGAGTCGGACAAGCTGCTCGACAACGAGCAGATCCAGCGTGAGGCGGTGGCGTCGGCGGAA
AATGACGGCATCGTTTTCCTGGACGAGATCGACAAGATCGCGACCCGTGAAGGCGGTATCGGCGCCGGCGTTTCACGCGA
GGGGGTGCAGCGCGACCTCCTGCCACTGGTCGAGGGCACGACGGTGGCCACGAAATACGGACCTGTGAAGACCGATCACA
TCCTGTTTATCGCTTCAGGTGCCTTCCACGTCGCCAAACCCTCGGATCTGCTGCCGGAGCTGCAGGGTCGTCTGCCGATC
CGCGTGGAGCTTCGCGCGCTCACGAAGGAGGATTTCCGCCGAATCCTGACGGAGACGGAAGCGAGTCTCATCCGTCAGTA
CAAGGCGCTGCTCGAAACCGAGGGAGTGACCCTCGATTTCACCGAGGACGCGATCGATGCGCTCGCGGAAGTCGCGGTCC
AGCTTAACTCCAATGTCGAGAATATCGGCGCACGCCGCCTGCAGACGGTGATGGAGCGCGTGCTCGACGACGTCTCCTTC
AATGCTCCCGATCGCGGCGGCCAGGGGGTCACGATCGATGCCGATTACGTCCGCGTGCATGTCGGCGATCTGGCGGCAAA
TACCGACCTGTCGCGCTACATCCTCTGA

Upstream 100 bases:

>100_bases
CCTATTCGAAGGCCGGTTTCGTACCCTTTGGCGAATGGATCGACAAGTCCGGTCGAACCCTCCTTATGGAGCTCAAGCGG
ACGGAATTTGAGAGGAATTG

Downstream 100 bases:

>100_bases
CCCTTCATTCGTCAGGGACTGGTCGATCACCGAATTGTGGGGCGCCTGTCACTCGACAGGCGCCTTCATTTTTTCTAGAC
CGTCAGCAGCCGTTAAGAAT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSSFSPREIVSELDRYIIGQKDAKRAVAIALRNRWRRQQLSDELRDEVMPKNILMIGPTGVGKTEISRRLAKLAGAPFVK
VEATKFTEVGYVGRDVEQIVRDLVEVGISLVREKRRAEVKAKAHQNAEERVLDALVGTTASPATRDSFRKKLRANELDDK
EIEVDVAETGNPGGAFEIPGMPGANIGVLNLSEMFGKALGGRTRKIKTTVKDSYALLVNDESDKLLDNEQIQREAVASAE
NDGIVFLDEIDKIATREGGIGAGVSREGVQRDLLPLVEGTTVATKYGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPI
RVELRALTKEDFRRILTETEASLIRQYKALLETEGVTLDFTEDAIDALAEVAVQLNSNVENIGARRLQTVMERVLDDVSF
NAPDRGGQGVTIDADYVRVHVGDLAANTDLSRYIL

Sequences:

>Translated_435_residues
MSSFSPREIVSELDRYIIGQKDAKRAVAIALRNRWRRQQLSDELRDEVMPKNILMIGPTGVGKTEISRRLAKLAGAPFVK
VEATKFTEVGYVGRDVEQIVRDLVEVGISLVREKRRAEVKAKAHQNAEERVLDALVGTTASPATRDSFRKKLRANELDDK
EIEVDVAETGNPGGAFEIPGMPGANIGVLNLSEMFGKALGGRTRKIKTTVKDSYALLVNDESDKLLDNEQIQREAVASAE
NDGIVFLDEIDKIATREGGIGAGVSREGVQRDLLPLVEGTTVATKYGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPI
RVELRALTKEDFRRILTETEASLIRQYKALLETEGVTLDFTEDAIDALAEVAVQLNSNVENIGARRLQTVMERVLDDVSF
NAPDRGGQGVTIDADYVRVHVGDLAANTDLSRYIL
>Mature_434_residues
SSFSPREIVSELDRYIIGQKDAKRAVAIALRNRWRRQQLSDELRDEVMPKNILMIGPTGVGKTEISRRLAKLAGAPFVKV
EATKFTEVGYVGRDVEQIVRDLVEVGISLVREKRRAEVKAKAHQNAEERVLDALVGTTASPATRDSFRKKLRANELDDKE
IEVDVAETGNPGGAFEIPGMPGANIGVLNLSEMFGKALGGRTRKIKTTVKDSYALLVNDESDKLLDNEQIQREAVASAEN
DGIVFLDEIDKIATREGGIGAGVSREGVQRDLLPLVEGTTVATKYGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPIR
VELRALTKEDFRRILTETEASLIRQYKALLETEGVTLDFTEDAIDALAEVAVQLNSNVENIGARRLQTVMERVLDDVSFN
APDRGGQGVTIDADYVRVHVGDLAANTDLSRYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=108, Percent_Identity=35.1851851851852, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1790366, Length=447, Percent_Identity=58.8366890380313, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1786642, Length=102, Percent_Identity=45.0980392156863, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319704, Length=141, Percent_Identity=32.6241134751773, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648291, Length=246, Percent_Identity=32.1138211382114, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24648289, Length=246, Percent_Identity=32.1138211382114, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 47810; Mature: 47679

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFSPREIVSELDRYIIGQKDAKRAVAIALRNRWRRQQLSDELRDEVMPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
VGKTEISRRLAKLAGAPFVKVEATKFTEVGYVGRDVEQIVRDLVEVGISLVREKRRAEVK
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKAHQNAEERVLDALVGTTASPATRDSFRKKLRANELDDKEIEVDVAETGNPGGAFEIPG
HHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEECCC
MPGANIGVLNLSEMFGKALGGRTRKIKTTVKDSYALLVNDESDKLLDNEQIQREAVASAE
CCCCCEEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCHHHHCHHHHHHHHHHCCC
NDGIVFLDEIDKIATREGGIGAGVSREGVQRDLLPLVEGTTVATKYGPVKTDHILFIASG
CCCEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECC
AFHVAKPSDLLPELQGRLPIRVELRALTKEDFRRILTETEASLIRQYKALLETEGVTLDF
CEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TEDAIDALAEVAVQLNSNVENIGARRLQTVMERVLDDVSFNAPDRGGQGVTIDADYVRVH
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEE
VGDLAANTDLSRYIL
ECCHHCCCCHHHHCC
>Mature Secondary Structure 
SSFSPREIVSELDRYIIGQKDAKRAVAIALRNRWRRQQLSDELRDEVMPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
VGKTEISRRLAKLAGAPFVKVEATKFTEVGYVGRDVEQIVRDLVEVGISLVREKRRAEVK
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKAHQNAEERVLDALVGTTASPATRDSFRKKLRANELDDKEIEVDVAETGNPGGAFEIPG
HHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEECCC
MPGANIGVLNLSEMFGKALGGRTRKIKTTVKDSYALLVNDESDKLLDNEQIQREAVASAE
CCCCCEEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCHHHHCHHHHHHHHHHCCC
NDGIVFLDEIDKIATREGGIGAGVSREGVQRDLLPLVEGTTVATKYGPVKTDHILFIASG
CCCEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECC
AFHVAKPSDLLPELQGRLPIRVELRALTKEDFRRILTETEASLIRQYKALLETEGVTLDF
CEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TEDAIDALAEVAVQLNSNVENIGARRLQTVMERVLDDVSFNAPDRGGQGVTIDADYVRVH
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEE
VGDLAANTDLSRYIL
ECCHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]