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The map label for this gene is mltA [H]

Identifier: 150398409

GI number: 150398409

Start: 3390954

End: 3392072

Strand: Direct

Name: mltA [H]

Synonym: Smed_3217

Alternate gene names: 150398409

Gene position: 3390954-3392072 (Clockwise)

Preceding gene: 150398408

Following gene: 150398410

Centisome position: 89.66

GC content: 62.73

Gene sequence:

>1119_bases
ATGGCTTTTGATCTCGAGCCGGTCTCCTTTTCGGAGCTGCCAGGCTGGTTTGCGGACGACCCGGCGCCGGTGATCGCTTC
ACTTCGCCGCTGTCACCGGCATGTAACGACGGTGAAGCCGTATAAGACCGGTTCGCTCGGGCTTTCGGTGGAGGACATGT
TGCCGGCCTTCGAGGCGGCGGGTGGGGATTTCGCCAATGCGGCGGCCGCACGTGCTTTTTTCGAGGCCTGGTTCGTTCCG
TTTCGTATCCGCCCGAAAGGGAAACGGGCGGGCTTCGTCACCGCCTTCTACGAGCCCGAGATCGAAGTCCGGGAAGAGCC
GGATGCGAATTTCCGCTTTCCATTATACCGGCGCCCCTCCGACCTCGTCGATATAAACGACGCAAACCGGCCGGAGGGGA
TGGACCCCTATTTCGCTTTCGGATGCCTTCGTGACGGCAGGATCGAAGAATATCCGGATCGCTGCGCCATCGAAGAGGGC
TTTCTCGCCGGGCGCGGGCTGGAGATCGCCTATGCCCGCTCCAAGGTCGACGTCTTTTTCGTCCATGTGCAGGGGGCAGC
CCGGCTGAACTATCCGGACGGGTCGCGTCGGCGCATCACCTATGCCGCAAAAACCGGGCATCGCTTCACCGCGATCGGCA
AACTGTTGATCGAGCGTGGAGAGATCGACGCCGCCACCGTGTCGATGGCCAGTATTCGTGAATGGCTCGCGGCCCATCCG
GACAGGACGGATGAGGTTCTTCGGCATAACCGGTCCTTCATCTTTTTCCGCGAGGCGCCGGTCGAGAAAGATGATCTCGG
CCCCGTCGCTGCTGCCAAGGTGCCGCTCGAGCCGGGCCGGTCGCTTGCGGTGGACCGACTCATCCACACTTTCGGCGTTC
CTTTTTACATCGCGAGCGAGACGCTGACCCATCTCGACGGAGGAAATTCCTTCGGCCGCCTGATGCTCGCGCTGGACACC
GGCTCTGCCATTGTCGGCCCGGCCCGTGGCGATATTTTCACCGGTTCCGGCGACGCGGCGGGGGATCTCGCCGGTTCCGT
GCGCAACGAAGCAGACTTCTTCATTCTCGTGCCCCTTGCGGCCGCGGCCCGATATCGCCGGCAGTCCAGTCATGGCTAG

Upstream 100 bases:

>100_bases
TGAACGATCTGTGGACCTTCTCGCGTGACATCCGTTCACGCGACCCGAACTGGAAGCTGATCGCTACCGAATCTGAAAAC
TGACCGGGAAGCGGGCGGCC

Downstream 100 bases:

>100_bases
GGAAAAGAAACTCTCCGCCGAGGACCGGATTCTCTGGGGGAAGGTGGCGCGCTCGGCGCGCCCGATGCCCGGGCGGCTGG
ATGATCTCGAGGAACTGCTC

Product: MltA domain-containing protein

Products: Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase A [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MAFDLEPVSFSELPGWFADDPAPVIASLRRCHRHVTTVKPYKTGSLGLSVEDMLPAFEAAGGDFANAAAARAFFEAWFVP
FRIRPKGKRAGFVTAFYEPEIEVREEPDANFRFPLYRRPSDLVDINDANRPEGMDPYFAFGCLRDGRIEEYPDRCAIEEG
FLAGRGLEIAYARSKVDVFFVHVQGAARLNYPDGSRRRITYAAKTGHRFTAIGKLLIERGEIDAATVSMASIREWLAAHP
DRTDEVLRHNRSFIFFREAPVEKDDLGPVAAAKVPLEPGRSLAVDRLIHTFGVPFYIASETLTHLDGGNSFGRLMLALDT
GSAIVGPARGDIFTGSGDAAGDLAGSVRNEADFFILVPLAAAARYRRQSSHG

Sequences:

>Translated_372_residues
MAFDLEPVSFSELPGWFADDPAPVIASLRRCHRHVTTVKPYKTGSLGLSVEDMLPAFEAAGGDFANAAAARAFFEAWFVP
FRIRPKGKRAGFVTAFYEPEIEVREEPDANFRFPLYRRPSDLVDINDANRPEGMDPYFAFGCLRDGRIEEYPDRCAIEEG
FLAGRGLEIAYARSKVDVFFVHVQGAARLNYPDGSRRRITYAAKTGHRFTAIGKLLIERGEIDAATVSMASIREWLAAHP
DRTDEVLRHNRSFIFFREAPVEKDDLGPVAAAKVPLEPGRSLAVDRLIHTFGVPFYIASETLTHLDGGNSFGRLMLALDT
GSAIVGPARGDIFTGSGDAAGDLAGSVRNEADFFILVPLAAAARYRRQSSHG
>Mature_371_residues
AFDLEPVSFSELPGWFADDPAPVIASLRRCHRHVTTVKPYKTGSLGLSVEDMLPAFEAAGGDFANAAAARAFFEAWFVPF
RIRPKGKRAGFVTAFYEPEIEVREEPDANFRFPLYRRPSDLVDINDANRPEGMDPYFAFGCLRDGRIEEYPDRCAIEEGF
LAGRGLEIAYARSKVDVFFVHVQGAARLNYPDGSRRRITYAAKTGHRFTAIGKLLIERGEIDAATVSMASIREWLAAHPD
RTDEVLRHNRSFIFFREAPVEKDDLGPVAAAKVPLEPGRSLAVDRLIHTFGVPFYIASETLTHLDGGNSFGRLMLALDTG
SAIVGPARGDIFTGSGDAAGDLAGSVRNEADFFILVPLAAAARYRRQSSHG

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi [H]

COG id: COG2821

COG function: function code M; Membrane-bound lytic murein transglycosylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789179, Length=273, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010611
- InterPro:   IPR014733
- InterPro:   IPR005300 [H]

Pfam domain/function: PF06725 3D; PF03562 MltA [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40961; Mature: 40829

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFDLEPVSFSELPGWFADDPAPVIASLRRCHRHVTTVKPYKTGSLGLSVEDMLPAFEAA
CCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHC
GGDFANAAAARAFFEAWFVPFRIRPKGKRAGFVTAFYEPEIEVREEPDANFRFPLYRRPS
CCCHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCCEECCCC
DLVDINDANRPEGMDPYFAFGCLRDGRIEEYPDRCAIEEGFLAGRGLEIAYARSKVDVFF
CEEECCCCCCCCCCCCHHEEEECCCCCCCCCCCHHCCCCCCCCCCCEEEEEECCCEEEEE
VHVQGAARLNYPDGSRRRITYAAKTGHRFTAIGKLLIERGEIDAATVSMASIREWLAAHP
EEEECCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
DRTDEVLRHNRSFIFFREAPVEKDDLGPVAAAKVPLEPGRSLAVDRLIHTFGVPFYIASE
CCHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEECC
TLTHLDGGNSFGRLMLALDTGSAIVGPARGDIFTGSGDAAGDLAGSVRNEADFFILVPLA
HHHHCCCCCCCCEEEEEEECCCEEECCCCCCEEECCCCCCHHHHHHCCCCCCEEEEEEHH
AAARYRRQSSHG
HHHHHHHHCCCC
>Mature Secondary Structure 
AFDLEPVSFSELPGWFADDPAPVIASLRRCHRHVTTVKPYKTGSLGLSVEDMLPAFEAA
CCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHC
GGDFANAAAARAFFEAWFVPFRIRPKGKRAGFVTAFYEPEIEVREEPDANFRFPLYRRPS
CCCHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCCEECCCC
DLVDINDANRPEGMDPYFAFGCLRDGRIEEYPDRCAIEEGFLAGRGLEIAYARSKVDVFF
CEEECCCCCCCCCCCCHHEEEECCCCCCCCCCCHHCCCCCCCCCCCEEEEEECCCEEEEE
VHVQGAARLNYPDGSRRRITYAAKTGHRFTAIGKLLIERGEIDAATVSMASIREWLAAHP
EEEECCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
DRTDEVLRHNRSFIFFREAPVEKDDLGPVAAAKVPLEPGRSLAVDRLIHTFGVPFYIASE
CCHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEECC
TLTHLDGGNSFGRLMLALDTGSAIVGPARGDIFTGSGDAAGDLAGSVRNEADFFILVPLA
HHHHCCCCCCCCEEEEEEECCCEEECCCCCCEEECCCCCCHHHHHHCCCCCCEEEEEEHH
AAARYRRQSSHG
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]