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The map label for this gene is azoR [H]

Identifier: 150396187

GI number: 150396187

Start: 1044014

End: 1044634

Strand: Direct

Name: azoR [H]

Synonym: Smed_0966

Alternate gene names: 150396187

Gene position: 1044014-1044634 (Clockwise)

Preceding gene: 150396185

Following gene: 150396188

Centisome position: 27.61

GC content: 60.55

Gene sequence:

>621_bases
ATGAAAATCCTTCATATCGACTCCGGCATTCTCGGCGAACACTCCGTCTCCCGCCGCCTGACGGCAGCGATCGTATCGCA
GATCAAGGCGGACCGACCCGACGCTGACATCACCTATAGGGATCTCGCATCCGAGCGCGTTCCGCACCTGACGGGCGCTC
AGATCATGGCGCCTGCCGATCTGGAAGGAGTGGATCCGGATCTTGCCGCTGACGTGCGCATCGGACGTCAGATGCTCGAG
GAATTCCTCGCCGCTGAAACGGTTGTCGTCGGTGCGCCGATGTACAACTTCTCGATCCCTAGCCAGCTCAAGGCCTGGAT
TGACCGTCTGGCGGTCGCCGGCAAGACCTTTCGCTATAGCGAAGCCGGAGCGGAAGGCCTGGCCAAGGGCAAGAAGGTGA
TCGTGGCCTCGACCCGTGGCGGTCACTATTCCGTGGCGCCGGCCTCCGCCATGGATCACCAGGAAACCTACCTGCGCTCG
GTCTTTGGCTTCTTCGGCATAACGGACATCGAGTTCATCCGCGCAGAGGGTCTGAACCTCGGCGCCGATCAGAAGCAGTT
CGCTATCGCGGAAGCCGAAAAGACGATCGCTGAGGGGAACGTTTTCAAGCTCGCAAGCTAA

Upstream 100 bases:

>100_bases
GTTTCCCTGAGGAAACGGAATGTCGAAGATTCACTGACTTATCGAGGGATTGTTTGCAGATCATATGTGATGCACCGCAA
CGAAAGCAAAGGTGCTTCCC

Downstream 100 bases:

>100_bases
GCAATTCCAGGAAAAGCGCATAGCGGTTTTCCATCCGGAGATACGGGCAACAAAGGGTGGAGCGTTTCAGTCATTCCACG
AATGCTGAACCCCCAGGAAT

Product: NAD(P)H dehydrogenase (quinone)

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKILHIDSGILGEHSVSRRLTAAIVSQIKADRPDADITYRDLASERVPHLTGAQIMAPADLEGVDPDLAADVRIGRQMLE
EFLAAETVVVGAPMYNFSIPSQLKAWIDRLAVAGKTFRYSEAGAEGLAKGKKVIVASTRGGHYSVAPASAMDHQETYLRS
VFGFFGITDIEFIRAEGLNLGADQKQFAIAEAEKTIAEGNVFKLAS

Sequences:

>Translated_206_residues
MKILHIDSGILGEHSVSRRLTAAIVSQIKADRPDADITYRDLASERVPHLTGAQIMAPADLEGVDPDLAADVRIGRQMLE
EFLAAETVVVGAPMYNFSIPSQLKAWIDRLAVAGKTFRYSEAGAEGLAKGKKVIVASTRGGHYSVAPASAMDHQETYLRS
VFGFFGITDIEFIRAEGLNLGADQKQFAIAEAEKTIAEGNVFKLAS
>Mature_206_residues
MKILHIDSGILGEHSVSRRLTAAIVSQIKADRPDADITYRDLASERVPHLTGAQIMAPADLEGVDPDLAADVRIGRQMLE
EFLAAETVVVGAPMYNFSIPSQLKAWIDRLAVAGKTFRYSEAGAEGLAKGKKVIVASTRGGHYSVAPASAMDHQETYLRS
VFGFFGITDIEFIRAEGLNLGADQKQFAIAEAEKTIAEGNVFKLAS

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=197, Percent_Identity=37.5634517766497, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 22219; Mature: 22219

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILHIDSGILGEHSVSRRLTAAIVSQIKADRPDADITYRDLASERVPHLTGAQIMAPAD
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEECCCC
LEGVDPDLAADVRIGRQMLEEFLAAETVVVGAPMYNFSIPSQLKAWIDRLAVAGKTFRYS
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECC
EAGAEGLAKGKKVIVASTRGGHYSVAPASAMDHQETYLRSVFGFFGITDIEFIRAEGLNL
HHCHHHHHCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
GADQKQFAIAEAEKTIAEGNVFKLAS
CCCCHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MKILHIDSGILGEHSVSRRLTAAIVSQIKADRPDADITYRDLASERVPHLTGAQIMAPAD
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEECCCC
LEGVDPDLAADVRIGRQMLEEFLAAETVVVGAPMYNFSIPSQLKAWIDRLAVAGKTFRYS
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECC
EAGAEGLAKGKKVIVASTRGGHYSVAPASAMDHQETYLRSVFGFFGITDIEFIRAEGLNL
HHCHHHHHCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
GADQKQFAIAEAEKTIAEGNVFKLAS
CCCCHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]