Click here to switch to the map view.

The map label for this gene is glyA [H]

Identifier: 150396040

GI number: 150396040

Start: 879563

End: 880858

Strand: Direct

Name: glyA [H]

Synonym: Smed_0816

Alternate gene names: 150396040

Gene position: 879563-880858 (Clockwise)

Preceding gene: 150396039

Following gene: 150396041

Centisome position: 23.26

GC content: 61.27

Gene sequence:

>1296_bases
ATGCCTTCACAGCCAAACGACGCTTTCTTCGCCCGCTCTCTCGCTGACAGCGACCCGGACATCTTCGGTGCGATCGAGAA
GGAGCTGGGTCGCCAGCGCCATGAGATCGAGCTGATTGCCTCCGAGAACATCGTCTCACGGGCCGTGCTCGAGGCGCAAG
GCTCCATCATGACCAACAAATACGCCGAAGGTTACCCGGGAAAGCGCTATTACGGCGGCTGCCAATATGTCGACATCGCC
GAGGAACTGGCGATCGAGCGCGCCAAGAAGCTCTTCGGCGTCGGCTTTGCCAACGTCCAGCCGAATTCGGGCTCGCAGAT
GAACCAGGCGGTTTTCCTGGCGCTCCTGCAGCCGGGCGATACCTTCATGGGCCTCGACCTCAATTCCGGCGGCCACCTCA
CGCACGGATCCCCGGTGAACATGTCGGGCAAATGGTTCAACGTCGTTTCCTACGGCGTGCGCGAAGACGATCACCTCCTC
GACATGGATGACGTTGCCAAGAAGGCGCGCGAGCATAAGCCGAAGCTGATCATCGCGGGCGGAACGGCCTATTCCCGGAT
CTGGGACTGGAAGCGCTTCCGCGAGATCGCCGACGAGGTTGGCGCCTGGCTGATGGTCGACATGGCTCATATTGCCGGGC
TCGTCGCCGGTGATCAGCACCCGTCGCCGTTTCCTCATTGCCACGTCGCGACGACGACCACCCACAAGTCTTTGCGCGGG
CCCCGCGGCGGCATGATCCTCACCAATGACGAGGAAATTGCAAAGAAGATCAATTCCGCGGTTTTCCCCGGCCTTCAGGG
CGGACCGCTGATGCACGTGATCGCAGCCAAGGCCGTCGCGTTCGGCGAAGCGCTGCAGCCTTCCTTCAAGGACTACGCGG
CTCAGGTTGTGAAGAATGCGCGCACGCTTGCCGACACGCTCAAGGCCAATGGGCTGGACATCGTCTCGGGCGGCACCGAC
AACCATCTGATGCTGGTGGATCTGCGCAAGAAGAACGCCACCGGCAAGCGTGCCGAAGCTGCGCTTGGCCGCGCCTATGT
CACCTGCAACAAGAACGGCATTCCCTTTGACCCGGAAAAACCCTTCGTCACATCAGGCGTTCGCCTGGGCACACCGGCCG
GGACGACGCGCGGTTTCAAGGAAGCGGAATTCAAGGAGATCGGCGAATTGATCGTCGAGGTCCTCGACGGCCTCAAGGCT
GCCAATTCCGATGAAGGCAATGCTGCCGTCGAGGCCGGCGTGCGCGAGAAGGTGATGAAGCTCACCGGCCGTTTCCCAAT
GTATGGCTATATGTGA

Upstream 100 bases:

>100_bases
CGCGGCGGATGGCGGAAATGACGCAATCCGCACAGCGGATATTGCTCTCGGCGTGCGAGGAAGCTAAAGAACAGCCACCC
TTCAGAGGAGCCTCGAGCCG

Downstream 100 bases:

>100_bases
TCGGACGGCTGCATGCGCTGCCCCTATTGCGGTTCCGAAGACAGTCAGGTGAAGGATTCCCGTCCGGCGGAGGACGGGAA
CGCCATTCGCCGCCGCCGCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPSQPNDAFFARSLADSDPDIFGAIEKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQYVDIA
EELAIERAKKLFGVGFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREDDHLL
DMDDVAKKAREHKPKLIIAGGTAYSRIWDWKRFREIADEVGAWLMVDMAHIAGLVAGDQHPSPFPHCHVATTTTHKSLRG
PRGGMILTNDEEIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPSFKDYAAQVVKNARTLADTLKANGLDIVSGGTD
NHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFKEAEFKEIGELIVEVLDGLKA
ANSDEGNAAVEAGVREKVMKLTGRFPMYGYM

Sequences:

>Translated_431_residues
MPSQPNDAFFARSLADSDPDIFGAIEKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQYVDIA
EELAIERAKKLFGVGFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREDDHLL
DMDDVAKKAREHKPKLIIAGGTAYSRIWDWKRFREIADEVGAWLMVDMAHIAGLVAGDQHPSPFPHCHVATTTTHKSLRG
PRGGMILTNDEEIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPSFKDYAAQVVKNARTLADTLKANGLDIVSGGTD
NHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFKEAEFKEIGELIVEVLDGLKA
ANSDEGNAAVEAGVREKVMKLTGRFPMYGYM
>Mature_430_residues
PSQPNDAFFARSLADSDPDIFGAIEKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQYVDIAE
ELAIERAKKLFGVGFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREDDHLLD
MDDVAKKAREHKPKLIIAGGTAYSRIWDWKRFREIADEVGAWLMVDMAHIAGLVAGDQHPSPFPHCHVATTTTHKSLRGP
RGGMILTNDEEIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPSFKDYAAQVVKNARTLADTLKANGLDIVSGGTDN
HLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFKEAEFKEIGELIVEVLDGLKAA
NSDEGNAAVEAGVREKVMKLTGRFPMYGYM

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=455, Percent_Identity=45.7142857142857, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI261862352, Length=455, Percent_Identity=45.7142857142857, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI261862350, Length=455, Percent_Identity=45.7142857142857, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI261862348, Length=455, Percent_Identity=45.7142857142857, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI261862346, Length=455, Percent_Identity=45.2747252747253, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI22547186, Length=464, Percent_Identity=45.9051724137931, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI22547189, Length=450, Percent_Identity=43.3333333333333, Blast_Score=313, Evalue=1e-85,
Organism=Escherichia coli, GI1788902, Length=418, Percent_Identity=59.8086124401914, Blast_Score=488, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI25144732, Length=423, Percent_Identity=47.0449172576832, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI25144729, Length=423, Percent_Identity=47.0449172576832, Blast_Score=360, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=420, Percent_Identity=44.047619047619, Blast_Score=352, Evalue=7e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=417, Percent_Identity=44.3645083932854, Blast_Score=335, Evalue=6e-93,
Organism=Drosophila melanogaster, GI24640005, Length=454, Percent_Identity=43.3920704845815, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI221329721, Length=454, Percent_Identity=43.3920704845815, Blast_Score=354, Evalue=8e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46719; Mature: 46588

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSQPNDAFFARSLADSDPDIFGAIEKELGRQRHEIELIASENIVSRAVLEAQGSIMTNK
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHEEEEEECCCHHHHHHHHHCCCEEECC
YAEGYPGKRYYGGCQYVDIAEELAIERAKKLFGVGFANVQPNSGSQMNQAVFLALLQPGD
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEEECCCC
TFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREDDHLLDMDDVAKKAREHKPKLIIAG
EEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEECHHHHHHHHHHCCCCEEEEC
GTAYSRIWDWKRFREIADEVGAWLMVDMAHIAGLVAGDQHPSPFPHCHVATTTTHKSLRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCC
PRGGMILTNDEEIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPSFKDYAAQVVKNA
CCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
RTLADTLKANGLDIVSGGTDNHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEK
HHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCCCCCC
PFVTSGVRLGTPAGTTRGFKEAEFKEIGELIVEVLDGLKAANSDEGNAAVEAGVREKVMK
CCEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LTGRFPMYGYM
HHCCCCCCCCC
>Mature Secondary Structure 
PSQPNDAFFARSLADSDPDIFGAIEKELGRQRHEIELIASENIVSRAVLEAQGSIMTNK
CCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHEEEEEECCCHHHHHHHHHCCCEEECC
YAEGYPGKRYYGGCQYVDIAEELAIERAKKLFGVGFANVQPNSGSQMNQAVFLALLQPGD
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEEECCCC
TFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREDDHLLDMDDVAKKAREHKPKLIIAG
EEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEECHHHHHHHHHHCCCCEEEEC
GTAYSRIWDWKRFREIADEVGAWLMVDMAHIAGLVAGDQHPSPFPHCHVATTTTHKSLRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCC
PRGGMILTNDEEIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALQPSFKDYAAQVVKNA
CCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
RTLADTLKANGLDIVSGGTDNHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEK
HHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCCCCCC
PFVTSGVRLGTPAGTTRGFKEAEFKEIGELIVEVLDGLKAANSDEGNAAVEAGVREKVMK
CCEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LTGRFPMYGYM
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA