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The map label for this gene is barA [C]

Identifier: 150395841

GI number: 150395841

Start: 664192

End: 665769

Strand: Direct

Name: barA [C]

Synonym: Smed_0617

Alternate gene names: 150395841

Gene position: 664192-665769 (Clockwise)

Preceding gene: 150395838

Following gene: 150395842

Centisome position: 17.56

GC content: 62.93

Gene sequence:

>1578_bases
ATGGCTGGCAAATGGTTATCCCTCGTGGATGACGCGGCCGCACCTTGGCTGCCACGCAGCGTTCTCGCCCCGGCAATCGT
ACGTCATGACTTCAGACGCCTGCGCACCGTCGCTGCCGTGTCCTTGACCGCGCTGCCGATCATCCCCCTCGCGCTGACGC
TGGCACTGCCCGTATCCGCCGCGCTGCCGGCGGGAGCGGCGCTTTGGGCGTCGGCCTCCCTGTTGGCCGCAGCGGCGGCT
ATTTCCGGCGGCCGTGCCGAGCCGATCGTCGCTGAGGTGGAAGTGGCGCCTGCGCCGGGCCTGCCAGACCTCGATACAGC
CTATGATCTGTTTGCGGGGTTGGTGACTGTCCACGATATCAGGGGCCACGTCCGGTCAGTACACGGCCGCGACGCCCGCG
AATATCTGAAACTGATGCGCGATCCGCAGGGGCGCGGCTTCATCGAGCAGATTCACGTTTCGGATCGTATCGCTTTCCTG
CGAGCGATCGATTCACTGCGCCTCGACAGCGAGCGCACGGCTGTGGACATTCGTCTCGAACGGACGTCGGCGGATGGTCC
CCAATTCGCTCATTTCTATTGCGAGATGACGCCCCTTCGGGACGCTGAAGGAAATCTCCTCGCGATTGTCGCCCAGTCGC
GCGATGTCTCCGAAGGCGCGCGTTTGCAGGCGGAGGCGGCAGCCAGGGCCGCGCACGCCGAGTCCGCAAACGATGCCAAG
ACCCGGTTCCTTGCCGCGGTCAGCCACGAATTGCGCACGCCGCTCAATGCCATTCTCGGTTTTTCCGACGTTCTTGCCGG
TGAATATTTCGGGAAACTCGAAAACGATCGCCAGCGCGAATATGTGTCGCTGATCCACCAGTCCGGCACGCATCTTCTCT
CGGTCGTCAACACGATGCTCGACATGAGCAAGATCGAAGCGGGCCGCTACGAGCTTCTCCGCGAACCTTTCCGCGTCGCC
GAGGCCATCGCCGCATGCGAGGCGATGCTGTCGCACCAGGCGCGCGAGAAGGGTGTGCGACTGGCGAGCCGGGTTACCCG
TTCGGTTGGCGAAATCAATGCAGACCAGCGCGCCGTCCAGCAAATTCTCATCAATCTCGTTGGTAACGCGATCAAGTTTA
CCGACCGGGGTGGGCTTGTCACCGTCGATGCCGCCCGGGAAGGCGCCTTGCTGAAGCTTACGGTCAGTGATACCGGAATC
GGCATCGCCGCGGACAAGCTGAAGATGCTTGGCCAACCTTTTGTCCAGATTCAGAATGATTATACCCGCCGCTACGAGGG
AACGGGACTTGGGCTGTCTCTGGTGAAGGGACTTGCCGAACTGCATGGCGGCGACGTTTCGATCCGTAGCGCCGAGGGTG
AGGGCACCGAGGTCGTGGTCTCCATTCCCTGCGATGTTTCGAGCATTGCCGGAGCGGAATGCGCGCGCGTGCCTGTCACG
GTGGAGTTTCCGCCGCGGTTGACGCTGCGCGGCGACGGAAAAAGCGATGTTGGTGAGCCGGCCGTGGTCGAGGCTCTCCG
CAAAGGAGAAATGGGTCGGGAAGGGGGACATGGCACCGCGCAAGCGAAAACAGCCTGA

Upstream 100 bases:

>100_bases
GTCACACGAGGCGGTCGTCCGCGACGACGCGGACAAGTCCGATAAACGCCTCCCAAGAACAACAAATGCGGCAGGGCAAG
GCGTGGGTCTATTGCGTGAC

Downstream 100 bases:

>100_bases
GCGTAACCGGGCGGCGCGGCGGGGTGCGGGTCTTGCCTTGCGCGGACTCGCTTTGGTCGGCCGTGGGCTGGTGCTCGCCG
GCCGCCTGGTCTCCCGTCAT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MAGKWLSLVDDAAAPWLPRSVLAPAIVRHDFRRLRTVAAVSLTALPIIPLALTLALPVSAALPAGAALWASASLLAAAAA
ISGGRAEPIVAEVEVAPAPGLPDLDTAYDLFAGLVTVHDIRGHVRSVHGRDAREYLKLMRDPQGRGFIEQIHVSDRIAFL
RAIDSLRLDSERTAVDIRLERTSADGPQFAHFYCEMTPLRDAEGNLLAIVAQSRDVSEGARLQAEAAARAAHAESANDAK
TRFLAAVSHELRTPLNAILGFSDVLAGEYFGKLENDRQREYVSLIHQSGTHLLSVVNTMLDMSKIEAGRYELLREPFRVA
EAIAACEAMLSHQAREKGVRLASRVTRSVGEINADQRAVQQILINLVGNAIKFTDRGGLVTVDAAREGALLKLTVSDTGI
GIAADKLKMLGQPFVQIQNDYTRRYEGTGLGLSLVKGLAELHGGDVSIRSAEGEGTEVVVSIPCDVSSIAGAECARVPVT
VEFPPRLTLRGDGKSDVGEPAVVEALRKGEMGREGGHGTAQAKTA

Sequences:

>Translated_525_residues
MAGKWLSLVDDAAAPWLPRSVLAPAIVRHDFRRLRTVAAVSLTALPIIPLALTLALPVSAALPAGAALWASASLLAAAAA
ISGGRAEPIVAEVEVAPAPGLPDLDTAYDLFAGLVTVHDIRGHVRSVHGRDAREYLKLMRDPQGRGFIEQIHVSDRIAFL
RAIDSLRLDSERTAVDIRLERTSADGPQFAHFYCEMTPLRDAEGNLLAIVAQSRDVSEGARLQAEAAARAAHAESANDAK
TRFLAAVSHELRTPLNAILGFSDVLAGEYFGKLENDRQREYVSLIHQSGTHLLSVVNTMLDMSKIEAGRYELLREPFRVA
EAIAACEAMLSHQAREKGVRLASRVTRSVGEINADQRAVQQILINLVGNAIKFTDRGGLVTVDAAREGALLKLTVSDTGI
GIAADKLKMLGQPFVQIQNDYTRRYEGTGLGLSLVKGLAELHGGDVSIRSAEGEGTEVVVSIPCDVSSIAGAECARVPVT
VEFPPRLTLRGDGKSDVGEPAVVEALRKGEMGREGGHGTAQAKTA
>Mature_524_residues
AGKWLSLVDDAAAPWLPRSVLAPAIVRHDFRRLRTVAAVSLTALPIIPLALTLALPVSAALPAGAALWASASLLAAAAAI
SGGRAEPIVAEVEVAPAPGLPDLDTAYDLFAGLVTVHDIRGHVRSVHGRDAREYLKLMRDPQGRGFIEQIHVSDRIAFLR
AIDSLRLDSERTAVDIRLERTSADGPQFAHFYCEMTPLRDAEGNLLAIVAQSRDVSEGARLQAEAAARAAHAESANDAKT
RFLAAVSHELRTPLNAILGFSDVLAGEYFGKLENDRQREYVSLIHQSGTHLLSVVNTMLDMSKIEAGRYELLREPFRVAE
AIAACEAMLSHQAREKGVRLASRVTRSVGEINADQRAVQQILINLVGNAIKFTDRGGLVTVDAAREGALLKLTVSDTGIG
IAADKLKMLGQPFVQIQNDYTRRYEGTGLGLSLVKGLAELHGGDVSIRSAEGEGTEVVVSIPCDVSSIAGAECARVPVTV
EFPPRLTLRGDGKSDVGEPAVVEALRKGEMGREGGHGTAQAKTA

Specific function: Member Of The Two-Component Regulatory System Uvry/Bara Involved In The Regulation Of Carbon Metabolism Via The Csra/Csrb Regulatory System. Phosphorylates Uvry, Probably Via A Four-Step Phosphorelay. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAC (PAS-associated C-terminal) domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=275, Percent_Identity=32, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789149, Length=240, Percent_Identity=34.5833333333333, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI145693157, Length=250, Percent_Identity=35.2, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI87081816, Length=263, Percent_Identity=34.6007604562738, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788713, Length=285, Percent_Identity=28.4210526315789, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=29.3333333333333, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786912, Length=275, Percent_Identity=29.4545454545455, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=27.5555555555556, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788393, Length=255, Percent_Identity=25.0980392156863, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=25.4464285714286, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786783, Length=237, Percent_Identity=23.6286919831224, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789808, Length=245, Percent_Identity=26.1224489795918, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87082128, Length=224, Percent_Identity=25, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790861, Length=218, Percent_Identity=27.0642201834862, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787894, Length=256, Percent_Identity=25.78125, Blast_Score=62, Evalue=8e-11,
Organism=Escherichia coli, GI1790346, Length=226, Percent_Identity=23.8938053097345, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=147, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000700
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56013; Mature: 55881

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKWLSLVDDAAAPWLPRSVLAPAIVRHDFRRLRTVAAVSLTALPIIPLALTLALPVSA
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPAGAALWASASLLAAAAAISGGRAEPIVAEVEVAPAPGLPDLDTAYDLFAGLVTVHDI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
RGHVRSVHGRDAREYLKLMRDPQGRGFIEQIHVSDRIAFLRAIDSLRLDSERTAVDIRLE
HHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RTSADGPQFAHFYCEMTPLRDAEGNLLAIVAQSRDVSEGARLQAEAAARAAHAESANDAK
ECCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHH
TRFLAAVSHELRTPLNAILGFSDVLAGEYFGKLENDRQREYVSLIHQSGTHLLSVVNTML
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
DMSKIEAGRYELLREPFRVAEAIAACEAMLSHQAREKGVRLASRVTRSVGEINADQRAVQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QILINLVGNAIKFTDRGGLVTVDAAREGALLKLTVSDTGIGIAADKLKMLGQPFVQIQND
HHHHHHHCCCEEEECCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHCCH
YTRRYEGTGLGLSLVKGLAELHGGDVSIRSAEGEGTEVVVSIPCDVSSIAGAECARVPVT
HHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCHHHHCCCCHHCCCEE
VEFPPRLTLRGDGKSDVGEPAVVEALRKGEMGREGGHGTAQAKTA
EECCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGKWLSLVDDAAAPWLPRSVLAPAIVRHDFRRLRTVAAVSLTALPIIPLALTLALPVSA
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPAGAALWASASLLAAAAAISGGRAEPIVAEVEVAPAPGLPDLDTAYDLFAGLVTVHDI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
RGHVRSVHGRDAREYLKLMRDPQGRGFIEQIHVSDRIAFLRAIDSLRLDSERTAVDIRLE
HHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RTSADGPQFAHFYCEMTPLRDAEGNLLAIVAQSRDVSEGARLQAEAAARAAHAESANDAK
ECCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHH
TRFLAAVSHELRTPLNAILGFSDVLAGEYFGKLENDRQREYVSLIHQSGTHLLSVVNTML
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
DMSKIEAGRYELLREPFRVAEAIAACEAMLSHQAREKGVRLASRVTRSVGEINADQRAVQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QILINLVGNAIKFTDRGGLVTVDAAREGALLKLTVSDTGIGIAADKLKMLGQPFVQIQND
HHHHHHHCCCEEEECCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHCCH
YTRRYEGTGLGLSLVKGLAELHGGDVSIRSAEGEGTEVVVSIPCDVSSIAGAECARVPVT
HHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCHHHHCCCCHHCCCEE
VEFPPRLTLRGDGKSDVGEPAVVEALRKGEMGREGGHGTAQAKTA
EECCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11481430; 7756693 [H]