Click here to switch to the map view.

The map label for this gene is kefA [H]

Identifier: 150395777

GI number: 150395777

Start: 599990

End: 602614

Strand: Direct

Name: kefA [H]

Synonym: Smed_0553

Alternate gene names: 150395777

Gene position: 599990-602614 (Clockwise)

Preceding gene: 150395775

Following gene: 150395779

Centisome position: 15.86

GC content: 62.21

Gene sequence:

>2625_bases
ATGACGAGCATGCGGATCCCCCATCGTCGCACGATTGCGGCGGGCCCGGCCTTCGAAAGAGCTTTGGACCAGGTCCGTTG
CCCCAGCACATGGCTTCGATGGTTATTCCTCGCGTTCTTTCTGGCATTTTCATCAGTAACTTTTGCCCAGGACGCAACAA
AACCGGAAGCCGGCGCGCCAACCGAAGCCCAGGAAAAGACCCGGGCGGATGCGGTGCCCGAAGTGCCACCGCAAGCTTTT
CCGGAGCAGCAGGCGCGTATCGATGCGTGGCAGGCGACACTGGAGCGCGTGGAGCGCGCCCTTCAGGCCGAGAGCATTGA
CGAGGCCACCCTGTCCGAACTGCGCAGCCAGCTCGCCAAGATCCCCGTGCAGGCTGCCGGGCTCAAGGTGAGCCTTCAGC
CACGGCTGCAGGTGGTCAACCAACGCATCGATCAGTTGAAGCCGGCCGATGAGCAGGCTGCCCGAAATCAGACCGAAGCG
ATCAAGCTCGAGCAGGCGCAGCTGCAATCAGAGGCGGCCGGGCTGCAGGGGGTCGTGCAGCAGGCGGATCTCATCGCTCT
TCGCGCGCAGCAGGATATCGATGCTATCGGTGAACGGCGGCGCGCCCTCTTTACAAGCTCCATCCTGCAGCGATCGCAAA
GCCTTGCCGATCCATCCTTTTGGCTCGAACTGGCCGCCGCGACTCCGGAGACGCTCGCAAATCAGTCCCGCGTCGTCGGG
CATTGGTTCGGCGTCCTGGTGGAGCGCACCGGCCGTTCGGCCGCCGGCATTCTCGTCGTTGTCGTCGGCTTCGCCGCCTT
TCTCCTTTCACCGGGCCGACGCTGGCTGTCCAGGCGCACGAGCCGCGATCCTTCGGTGCTCTACCCGTCCAGGCTCGCCA
AGGCGACCGCGGCAGCAGCCATCACACTTGCCAACCTCCTGATCCCGGCAATCGCCTTCTTCATGCTTTATCAGGCGATG
TCCATTCTGAGGGTCCTTCCTGTCGATCTGGCGACGGTCCTCAGACCGGTTTTTTTCGGCTTGACCTTCGCATCCTTTTT
TTACGGCCTTTCAATTGCTGTGCTCGCGCCCGAACGGCCGAGCTGGCGGCTCGTCGATATTACCGATGCGGCAGCCGAGC
GGCTGCTCCCCGTCATCGTCGCGATGGCAGTGGTCAATGCCGGCGGCCTTGCCCTCGATGCCTTCCTGAAAGCTACCTAT
GCGCCCTTGTCCTTTGCCGTTGCGGCACAAGGCCTCGGCGCCATCACGCTCGGCATTCTCGCCATGGTGGCGCTCAGATT
CGTGGCCCGCGAGGAAAACGACGAGGATACGCAGAGAACGAGTTCGGCCTGGCGCCTGCTCATTCCGGTGACCTGGGCGG
TCGCGGCGCTGACCGTCGTTGCGCCACTCGCCGGCTTTGTAGCCTTTGGCCGCTTTGCCGCGCTGCAGATCGTCTTCACG
ACGGCGGTGCTGATGAGCGTGGTGCTTATGCTGCGCCTGGCGGATGAGGCGATTTCATACGGGTTTTCCGTCCAGACGCG
GATCGGCGGCTTCATGCGGCAGGCGATCGGCCTCAAGACCAACACGATAAGCCAGATCGGCGTGATTCTCGCAGGTTTCG
CGCGGCTGGTCGTGTTGATGATTGCCGTAATGGCTCTTCTCGCGCCGTGGGGCATCCAGTCGGAGGACCTGGTCGGCACC
TTCACTTCCGCCTTTTTCGGTTTCGAGATCGCCGGCTTTTCCTTCTCGCCATCCGCCATTCTCGGCGCTATCGTCGTCTT
CATCATCGTAATCGTCGCCACGCGCGGCTTCCAGAGATGGCTCGACGGCCGGCTCCTGCCGCAAACGAGCCTCGACACAG
GACTGAGGACGTCGATCCACACCGGGGTCGGCTATATCGGGGCGGCGATCGCCGGGCTAGTGGCTTTCTCCTATGCGGGC
TTGAACCTTCAGAACGTCGCCATCGTTGCAAGCGCACTGTCCGTGGGTATCGGTTTCGGCCTTCAGTCCATCGTCAACAA
TTTCGTTTCGGGTATCATTCTCCTCGCCGAACGGCCCTTCAAAGTCGGCGATCGCATCGAGGTCGGCCAGAACATGGGGA
TCGTCCAGCGCGTCAGCGTCAGGGCGACCCAGATCCAGACCTTCGACAATGTGACGGTGATCGTACCCAATGCCGATCTT
ATCAGCGGCCAGGTGGTCAACTGGATGCATGGCGACTTCTCAGCGCGCCTGAAGATTCCCGTGGGGGTCTCCTATGATTC
GGACCCGGAGCAGGTAAAACGGGTGTTGCTGGAGATCGCAAAGGACAATCCACGCGTCCTGCAAATTCCGGAGCCGTTCG
TTTCCTTCACCGATTTCGGAGCCGACGCCCTGCAGTTCACCTTGTTCTGCCACGTCGGCAACATCAACGCCGATGCGGGC
GCGGCGAGCGATATGCGTTTCGAGATAGCGAAACGCTTTCGGGAGGAAAAAATCGAGATGCCCTTCGGCCAGCGCGACAT
CCATCTTCGCGACCTGGCGAGCATAGAAGGATTGGTCCGCGACCTGGTCGGCGCCCGCGCTGCCGCCCCGGCGACCGCTC
CTTCTCCCGCGGCAAAAGCCGGTGCGAAGCGTAAGAAAAGCCCGGCGGATGCCGCAGACGAATGA

Upstream 100 bases:

>100_bases
ATATTTCGGCCGGCAAAACTATTGTTCCCCTGCAAAATCACAGGCTCGGCTTTGCCACGCCCGGCCAACATACGATGAAG
CAAGTGGAATAGGACTGATA

Downstream 100 bases:

>100_bases
CATTGCGGCATGGAGAGCTGAGATCTCCTGATTGTGCAAGCGGCCCGCTGACCATCGAAAGCGGGCCGAAACACATATTG
CGCCGTGCGTCTTATCGGAC

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 874; Mature: 873

Protein sequence:

>874_residues
MTSMRIPHRRTIAAGPAFERALDQVRCPSTWLRWLFLAFFLAFSSVTFAQDATKPEAGAPTEAQEKTRADAVPEVPPQAF
PEQQARIDAWQATLERVERALQAESIDEATLSELRSQLAKIPVQAAGLKVSLQPRLQVVNQRIDQLKPADEQAARNQTEA
IKLEQAQLQSEAAGLQGVVQQADLIALRAQQDIDAIGERRRALFTSSILQRSQSLADPSFWLELAAATPETLANQSRVVG
HWFGVLVERTGRSAAGILVVVVGFAAFLLSPGRRWLSRRTSRDPSVLYPSRLAKATAAAAITLANLLIPAIAFFMLYQAM
SILRVLPVDLATVLRPVFFGLTFASFFYGLSIAVLAPERPSWRLVDITDAAAERLLPVIVAMAVVNAGGLALDAFLKATY
APLSFAVAAQGLGAITLGILAMVALRFVAREENDEDTQRTSSAWRLLIPVTWAVAALTVVAPLAGFVAFGRFAALQIVFT
TAVLMSVVLMLRLADEAISYGFSVQTRIGGFMRQAIGLKTNTISQIGVILAGFARLVVLMIAVMALLAPWGIQSEDLVGT
FTSAFFGFEIAGFSFSPSAILGAIVVFIIVIVATRGFQRWLDGRLLPQTSLDTGLRTSIHTGVGYIGAAIAGLVAFSYAG
LNLQNVAIVASALSVGIGFGLQSIVNNFVSGIILLAERPFKVGDRIEVGQNMGIVQRVSVRATQIQTFDNVTVIVPNADL
ISGQVVNWMHGDFSARLKIPVGVSYDSDPEQVKRVLLEIAKDNPRVLQIPEPFVSFTDFGADALQFTLFCHVGNINADAG
AASDMRFEIAKRFREEKIEMPFGQRDIHLRDLASIEGLVRDLVGARAAAPATAPSPAAKAGAKRKKSPADAADE

Sequences:

>Translated_874_residues
MTSMRIPHRRTIAAGPAFERALDQVRCPSTWLRWLFLAFFLAFSSVTFAQDATKPEAGAPTEAQEKTRADAVPEVPPQAF
PEQQARIDAWQATLERVERALQAESIDEATLSELRSQLAKIPVQAAGLKVSLQPRLQVVNQRIDQLKPADEQAARNQTEA
IKLEQAQLQSEAAGLQGVVQQADLIALRAQQDIDAIGERRRALFTSSILQRSQSLADPSFWLELAAATPETLANQSRVVG
HWFGVLVERTGRSAAGILVVVVGFAAFLLSPGRRWLSRRTSRDPSVLYPSRLAKATAAAAITLANLLIPAIAFFMLYQAM
SILRVLPVDLATVLRPVFFGLTFASFFYGLSIAVLAPERPSWRLVDITDAAAERLLPVIVAMAVVNAGGLALDAFLKATY
APLSFAVAAQGLGAITLGILAMVALRFVAREENDEDTQRTSSAWRLLIPVTWAVAALTVVAPLAGFVAFGRFAALQIVFT
TAVLMSVVLMLRLADEAISYGFSVQTRIGGFMRQAIGLKTNTISQIGVILAGFARLVVLMIAVMALLAPWGIQSEDLVGT
FTSAFFGFEIAGFSFSPSAILGAIVVFIIVIVATRGFQRWLDGRLLPQTSLDTGLRTSIHTGVGYIGAAIAGLVAFSYAG
LNLQNVAIVASALSVGIGFGLQSIVNNFVSGIILLAERPFKVGDRIEVGQNMGIVQRVSVRATQIQTFDNVTVIVPNADL
ISGQVVNWMHGDFSARLKIPVGVSYDSDPEQVKRVLLEIAKDNPRVLQIPEPFVSFTDFGADALQFTLFCHVGNINADAG
AASDMRFEIAKRFREEKIEMPFGQRDIHLRDLASIEGLVRDLVGARAAAPATAPSPAAKAGAKRKKSPADAADE
>Mature_873_residues
TSMRIPHRRTIAAGPAFERALDQVRCPSTWLRWLFLAFFLAFSSVTFAQDATKPEAGAPTEAQEKTRADAVPEVPPQAFP
EQQARIDAWQATLERVERALQAESIDEATLSELRSQLAKIPVQAAGLKVSLQPRLQVVNQRIDQLKPADEQAARNQTEAI
KLEQAQLQSEAAGLQGVVQQADLIALRAQQDIDAIGERRRALFTSSILQRSQSLADPSFWLELAAATPETLANQSRVVGH
WFGVLVERTGRSAAGILVVVVGFAAFLLSPGRRWLSRRTSRDPSVLYPSRLAKATAAAAITLANLLIPAIAFFMLYQAMS
ILRVLPVDLATVLRPVFFGLTFASFFYGLSIAVLAPERPSWRLVDITDAAAERLLPVIVAMAVVNAGGLALDAFLKATYA
PLSFAVAAQGLGAITLGILAMVALRFVAREENDEDTQRTSSAWRLLIPVTWAVAALTVVAPLAGFVAFGRFAALQIVFTT
AVLMSVVLMLRLADEAISYGFSVQTRIGGFMRQAIGLKTNTISQIGVILAGFARLVVLMIAVMALLAPWGIQSEDLVGTF
TSAFFGFEIAGFSFSPSAILGAIVVFIIVIVATRGFQRWLDGRLLPQTSLDTGLRTSIHTGVGYIGAAIAGLVAFSYAGL
NLQNVAIVASALSVGIGFGLQSIVNNFVSGIILLAERPFKVGDRIEVGQNMGIVQRVSVRATQIQTFDNVTVIVPNADLI
SGQVVNWMHGDFSARLKIPVGVSYDSDPEQVKRVLLEIAKDNPRVLQIPEPFVSFTDFGADALQFTLFCHVGNINADAGA
ASDMRFEIAKRFREEKIEMPFGQRDIHLRDLASIEGLVRDLVGARAAAPATAPSPAAKAGAKRKKSPADAADE

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=306, Percent_Identity=34.3137254901961, Blast_Score=173, Evalue=4e-44,
Organism=Escherichia coli, GI2367355, Length=304, Percent_Identity=30.9210526315789, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1789291, Length=255, Percent_Identity=24.7058823529412, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 94208; Mature: 94077

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00213 LIPOCALIN ; PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSMRIPHRRTIAAGPAFERALDQVRCPSTWLRWLFLAFFLAFSSVTFAQDATKPEAGAP
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TEAQEKTRADAVPEVPPQAFPEQQARIDAWQATLERVERALQAESIDEATLSELRSQLAK
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IPVQAAGLKVSLQPRLQVVNQRIDQLKPADEQAARNQTEAIKLEQAQLQSEAAGLQGVVQ
CCHHHCCEEEECCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
QADLIALRAQQDIDAIGERRRALFTSSILQRSQSLADPSFWLELAAATPETLANQSRVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCHHHHH
HWFGVLVERTGRSAAGILVVVVGFAAFLLSPGRRWLSRRTSRDPSVLYPSRLAKATAAAA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ITLANLLIPAIAFFMLYQAMSILRVLPVDLATVLRPVFFGLTFASFFYGLSIAVLAPERP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
SWRLVDITDAAAERLLPVIVAMAVVNAGGLALDAFLKATYAPLSFAVAAQGLGAITLGIL
CEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AMVALRFVAREENDEDTQRTSSAWRLLIPVTWAVAALTVVAPLAGFVAFGRFAALQIVFT
HHHHHHHHHHCCCCHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAVLMSVVLMLRLADEAISYGFSVQTRIGGFMRQAIGLKTNTISQIGVILAGFARLVVLM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IAVMALLAPWGIQSEDLVGTFTSAFFGFEIAGFSFSPSAILGAIVVFIIVIVATRGFQRW
HHHHHHHCCCCCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDGRLLPQTSLDTGLRTSIHTGVGYIGAAIAGLVAFSYAGLNLQNVAIVASALSVGIGFG
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LQSIVNNFVSGIILLAERPFKVGDRIEVGQNMGIVQRVSVRATQIQTFDNVTVIVPNADL
HHHHHHHHHHHHHHEECCCCCCCCCEECCCCCCHHHHHHHHHHHEEEECCEEEEECCCCH
ISGQVVNWMHGDFSARLKIPVGVSYDSDPEQVKRVLLEIAKDNPRVLQIPEPFVSFTDFG
HCCCHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCC
ADALQFTLFCHVGNINADAGAASDMRFEIAKRFREEKIEMPFGQRDIHLRDLASIEGLVR
HHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
DLVGARAAAPATAPSPAAKAGAKRKKSPADAADE
HHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSMRIPHRRTIAAGPAFERALDQVRCPSTWLRWLFLAFFLAFSSVTFAQDATKPEAGAP
CCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TEAQEKTRADAVPEVPPQAFPEQQARIDAWQATLERVERALQAESIDEATLSELRSQLAK
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IPVQAAGLKVSLQPRLQVVNQRIDQLKPADEQAARNQTEAIKLEQAQLQSEAAGLQGVVQ
CCHHHCCEEEECCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
QADLIALRAQQDIDAIGERRRALFTSSILQRSQSLADPSFWLELAAATPETLANQSRVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCHHHHH
HWFGVLVERTGRSAAGILVVVVGFAAFLLSPGRRWLSRRTSRDPSVLYPSRLAKATAAAA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ITLANLLIPAIAFFMLYQAMSILRVLPVDLATVLRPVFFGLTFASFFYGLSIAVLAPERP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
SWRLVDITDAAAERLLPVIVAMAVVNAGGLALDAFLKATYAPLSFAVAAQGLGAITLGIL
CEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AMVALRFVAREENDEDTQRTSSAWRLLIPVTWAVAALTVVAPLAGFVAFGRFAALQIVFT
HHHHHHHHHHCCCCHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAVLMSVVLMLRLADEAISYGFSVQTRIGGFMRQAIGLKTNTISQIGVILAGFARLVVLM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IAVMALLAPWGIQSEDLVGTFTSAFFGFEIAGFSFSPSAILGAIVVFIIVIVATRGFQRW
HHHHHHHCCCCCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDGRLLPQTSLDTGLRTSIHTGVGYIGAAIAGLVAFSYAGLNLQNVAIVASALSVGIGFG
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LQSIVNNFVSGIILLAERPFKVGDRIEVGQNMGIVQRVSVRATQIQTFDNVTVIVPNADL
HHHHHHHHHHHHHHEECCCCCCCCCEECCCCCCHHHHHHHHHHHEEEECCEEEEECCCCH
ISGQVVNWMHGDFSARLKIPVGVSYDSDPEQVKRVLLEIAKDNPRVLQIPEPFVSFTDFG
HCCCHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCC
ADALQFTLFCHVGNINADAGAASDMRFEIAKRFREEKIEMPFGQRDIHLRDLASIEGLVR
HHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
DLVGARAAAPATAPSPAAKAGAKRKKSPADAADE
HHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]