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The map label for this gene is rnhA [H]

Identifier: 150395753

GI number: 150395753

Start: 571035

End: 571496

Strand: Direct

Name: rnhA [H]

Synonym: Smed_0529

Alternate gene names: 150395753

Gene position: 571035-571496 (Clockwise)

Preceding gene: 150395752

Following gene: 150395756

Centisome position: 15.1

GC content: 60.82

Gene sequence:

>462_bases
ATGAAACACGTCGACATCTACACGGACGGTGCCTGCTCGGGAAATCCCGGGCCGGGCGGGTGGGGCGCGGTCCTGCGCTA
TGGCGACGTGGAAAAGGAGATGTCCGGCGGAGAAGCGGAGACGACGAACAACCGTATGGAACTTCTTGCCGCGATCTCGG
CACTGAACGCGCTCAGGCAGCCCTGCGAGGTCGATCTCCATACCGACAGCAAATATGTCATGGACGGCATCTCCAAATGG
ATACACGGCTGGAAGCGCAACGGCTGGAAAACCGGCGACAGAAAACCCGTGAAGAACGGCGAATTATGGCAGGCGCTCGA
CGCGGCGAGAGACCGCCACAACGTGACCTGGCACTGGGTCAAGGGTCACGCGGGACATCCGGAAAATGAACGGGCGGACG
AATTGGCCCGCAAGGGCATGGAACCGTTCAAGAGAGCGCATCGCGCCGATGCGGTGAAATAG

Upstream 100 bases:

>100_bases
TGGTCAAGAAGGATCCGCTCGAATATCTGACGAAGATCCGCTTCCATCGCGCCATCGTCTCCAGCGCCGAGTACGGCCTG
CGACGCGAAGAGGCATCGGC

Downstream 100 bases:

>100_bases
AAAGCTCTCCATTTGCCGCCGCGCCGTCATCCCGCTGCCGCGACCATCGCCCCGCCTGCGGGGAGAAGATAGCCGGCAGG
CCGGATGAGGGGGCAGGTTC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MKHVDIYTDGACSGNPGPGGWGAVLRYGDVEKEMSGGEAETTNNRMELLAAISALNALRQPCEVDLHTDSKYVMDGISKW
IHGWKRNGWKTGDRKPVKNGELWQALDAARDRHNVTWHWVKGHAGHPENERADELARKGMEPFKRAHRADAVK

Sequences:

>Translated_153_residues
MKHVDIYTDGACSGNPGPGGWGAVLRYGDVEKEMSGGEAETTNNRMELLAAISALNALRQPCEVDLHTDSKYVMDGISKW
IHGWKRNGWKTGDRKPVKNGELWQALDAARDRHNVTWHWVKGHAGHPENERADELARKGMEPFKRAHRADAVK
>Mature_153_residues
MKHVDIYTDGACSGNPGPGGWGAVLRYGDVEKEMSGGEAETTNNRMELLAAISALNALRQPCEVDLHTDSKYVMDGISKW
IHGWKRNGWKTGDRKPVKNGELWQALDAARDRHNVTWHWVKGHAGHPENERADELARKGMEPFKRAHRADAVK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=144, Percent_Identity=36.8055555555556, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1786408, Length=137, Percent_Identity=66.4233576642336, Blast_Score=199, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI115532542, Length=146, Percent_Identity=41.0958904109589, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17505482, Length=109, Percent_Identity=39.4495412844037, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45552509, Length=153, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137476, Length=153, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17124; Mature: 17124

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHVDIYTDGACSGNPGPGGWGAVLRYGDVEKEMSGGEAETTNNRMELLAAISALNALRQ
CCCEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
PCEVDLHTDSKYVMDGISKWIHGWKRNGWKTGDRKPVKNGELWQALDAARDRHNVTWHWV
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
KGHAGHPENERADELARKGMEPFKRAHRADAVK
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKHVDIYTDGACSGNPGPGGWGAVLRYGDVEKEMSGGEAETTNNRMELLAAISALNALRQ
CCCEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
PCEVDLHTDSKYVMDGISKWIHGWKRNGWKTGDRKPVKNGELWQALDAARDRHNVTWHWV
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
KGHAGHPENERADELARKGMEPFKRAHRADAVK
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]