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The map label for this gene is dfrA [H]

Identifier: 150392861

GI number: 150392861

Start: 411443

End: 412468

Strand: Direct

Name: dfrA [H]

Synonym: SaurJH1_0387

Alternate gene names: 150392861

Gene position: 411443-412468 (Clockwise)

Preceding gene: 150392860

Following gene: 150392866

Centisome position: 14.16

GC content: 35.28

Gene sequence:

>1026_bases
ATGAATAATAAAGTATTAGTAACCGGTGGTACAGGGTTTGTTGGCATGCGAATTATTTCACGATTATTAGAACAAGGTTA
TGAGGTACAAACAACGATACGTGATTTAAGTAAAGCTGATAAAGTAATTAAAACAATGCAAGACAATGGCATTTCCACAG
AGCGATTAATGTTTGTCGAAGCGGATTTATCACAAGATGAACATTGGGATGAAGCAATGAAAGATTGTAAATATGTCTTG
AGTGTAGCATCTCCGGTGTTTTTCGGTAAAACAGACGATGCAGAAGTGATGGCGAAGCCTGCCATTGAAGGCATACAACG
TATTTTAAGAGCTGCAGAACATGCTGGCGTGAAGCGTGTGGTGATGACTGCAAACTTTGGTGCAGTTGGTTTTAGCAATA
AAGATAAAAATTCAATCACAAATGAAAGTCATTGGACAAATGAAGATGAACCAGGCTTATCAGTATATGAAAAATCAAAA
TTGTTAGCTGAAAAGGCAGCGTGGGATTTTGTTGAGAATGAAAATACAATAGTAGAATTTGCCACAATCAATCCAGTTGC
AATTTTTGGACCATCATTAGATGCACACGTTTCAGGAAGTTTCCACTTGTTAGAAAACTTATTAAATGGTTCAATGAAAC
GTGTACCGCAAATTCCATTGAATGTTGTTGATGTGAGAGACGTAGCTGAACTACACATTTTGGCAATGACAAATGAACAG
GCTAATGGCAAGCGATTTATTGCTACAGCTGATGGACAAATTAATTTGTTGGAAATTGCAAAATTAATTAAAGAAAAGAG
ACCTGAAATAGCTCAAAAAGTTTCTACTAAAAAATTACCAGACTTTATTTTGAGTCTAGGTGCTAAATTTAATCATCAAG
CTAAAGAAGGTAAACTTTTATTAGATATGAACCGCAATGTAAGTAACGAACGTGCAAAAACATTACTTGGTTGGGAACCG
ATTGCGACACAAGAAGAAGCAATTTTAGCAGCAATTGATAGTATGGCTAAATTTCATTTAATATAA

Upstream 100 bases:

>100_bases
AAGTAAGATTAAATACTAGCTACTAAAGTCTATTTATATACGTCAAAATATAATGACTGCTATAATGAGTAATCAATAGA
CACAAAGAGGAGATTATGTG

Downstream 100 bases:

>100_bases
TACAAAAACACCGTCCATAAGTAAGTGGCTTTCCTCTTGATAAAAGAGGCATTCACTCAATATGTGACGGTGTTTTATAT
ATTTACGTTAACACTATTGC

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIKTMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVL
SVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
LLAEKAAWDFVENENTIVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEP
IATQEEAILAAIDSMAKFHLI

Sequences:

>Translated_341_residues
MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIKTMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVL
SVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
LLAEKAAWDFVENENTIVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEP
IATQEEAILAAIDSMAKFHLI
>Mature_341_residues
MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIKTMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVL
SVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
LLAEKAAWDFVENENTIVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEP
IATQEEAILAAIDSMAKFHLI

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=327, Percent_Identity=34.5565749235474, Blast_Score=174, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6320750, Length=341, Percent_Identity=30.2052785923754, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321399, Length=335, Percent_Identity=28.6567164179104, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324421, Length=338, Percent_Identity=28.1065088757396, Blast_Score=122, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6321281, Length=318, Percent_Identity=29.559748427673, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 37801; Mature: 37801

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIKTMQDNGISTERLMFVE
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
ADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRV
CCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
VMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSKLLAEKAAWDFVENENTIVEF
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
ATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
EECCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHCCCCCCEEEEHHHHHHEEEEEEECCC
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLL
CCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
LDMNRNVSNERAKTLLGWEPIATQEEAILAAIDSMAKFHLI
EEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIKTMQDNGISTERLMFVE
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
ADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRV
CCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
VMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSKLLAEKAAWDFVENENTIVEF
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
ATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
EECCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHCCCCCCEEEEHHHHHHEEEEEEECCC
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLL
CCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
LDMNRNVSNERAKTLLGWEPIATQEEAILAAIDSMAKFHLI
EEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]