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The map label for this gene is prtC [H]

Identifier: 150009776

GI number: 150009776

Start: 3862588

End: 3863847

Strand: Direct

Name: prtC [H]

Synonym: BDI_3191

Alternate gene names: 150009776

Gene position: 3862588-3863847 (Clockwise)

Preceding gene: 150009762

Following gene: 150009777

Centisome position: 80.28

GC content: 50.08

Gene sequence:

>1260_bases
TTGAAAAAGAAGGATTTTGAAATAATGGCGCCTGTCGGTTCCTATGAATCGTTGATGGCGGCTATACAAGGAGGGGCCGA
CTCCATTTATTTCGGTATCGAAGGATTGAATATGCGTTCTCGCTCGTCGAATAATTTCACGACGGAGGATTTGCGTAAGA
TCGTCGCTATTTGCGGCGAACATGGTATCAAGAGTTATCTCACGGTTAATACGGTTATTTATGGTGAGGATCTTCCGTTG
ATGAGGGAGATTATAGATGCCGCTAAGGATGCGGGTGTCTCCGCCATTATCGCCGCCGATGTAGCCGCTATGAGTTACGC
AAATAGCATCGGGCAAGAGGTGCACTTATCCACGCAATTGAATATCTCGAACGTGGAGGCGTTGAAGTTTTACGCCCGCT
TCGCCGATGTGGTCGTATTGGCTCGCGAATTGAACTTAAAGCAGGTGCATGAGATTTATCAGGAGATCGTGAAGCAGCAG
ATCAAGGGCCCGAAAGGGGAATTGATCCGCATAGAGATGTTCGCTCACGGGGCTTTGTGCATGGCCGTGTCCGGTAAATG
CTATTTGAGTTTGCATGAGATGAACGCTTCCGCCAACCGGGGGGCTTGCATGCAAATTTGTCGTCGTGCCTATACGGTGC
ATGATAAGGATAGCCAGATCGAGCTGGATGTGGAGAATCAGTATATCATGTCGCCGAAGGACTTGAAGACGATTCATTTC
ATGAATAAGATGATGGATGCCGGCGTACGTGTGTTCAAGCTGGAGGGGCGTGCCCGCGGGCCGGAGTATGTGCGTCTCGT
GACCGAATGTTATAAAGAGGCGGTCAAGGCTTATTGCGAAGGTACCTTCGATGAGGAAAAGATAGCGGTTTGGGACGAGC
GTCTGCGCAGGGTCTTTAATCGCGGTTTCTGGGATGGCTACTATCTGGGACAACGATTGGGTGAATGGAGCAGTAAATAT
GGCTCCGGTGCTACCCGTAAGAAAGTGTACGTGGCACGGGGTATCAAATACTTCAGCGGAATCGGTGTCGCCGAGTTTGA
GATGGAGTCCGGTTCCTTGAGAGTAGGCGATGAGATCTTGGTAACGGGTCCCACGACCGGAGCCGTTATGCAGACCGTCG
ATGAGATCCGGGTAGACCTGAAACCGGTGGAGGAGACCGTGAAAGGGGAACGCTTCTCGATAAAGATGTCCGAGAAGATC
CGCCCGTCCGATCGTCTTTATAAAATGGAGAAAGTACAAATCGAAAAAGAACTACGATAA

Upstream 100 bases:

>100_bases
TTGCCTGTCTTTCCTCTTTCTGTGGCCTGTGGGTCGTTTATAAGAAATCTTCTCAAATATTATTCGTACCTTTGCTACCG
TAATATTAAAATATATAACC

Downstream 100 bases:

>100_bases
TGAAGGAATATATTTTTAAACTGACAGACAAGGTTCGCGATTACGAGTGCGATTTGCAAGGCGTGGTGAATAACTCCAAT
TACCAGCATTATATGGAGCA

Product: collagenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKKKDFEIMAPVGSYESLMAAIQGGADSIYFGIEGLNMRSRSSNNFTTEDLRKIVAICGEHGIKSYLTVNTVIYGEDLPL
MREIIDAAKDAGVSAIIAADVAAMSYANSIGQEVHLSTQLNISNVEALKFYARFADVVVLARELNLKQVHEIYQEIVKQQ
IKGPKGELIRIEMFAHGALCMAVSGKCYLSLHEMNASANRGACMQICRRAYTVHDKDSQIELDVENQYIMSPKDLKTIHF
MNKMMDAGVRVFKLEGRARGPEYVRLVTECYKEAVKAYCEGTFDEEKIAVWDERLRRVFNRGFWDGYYLGQRLGEWSSKY
GSGATRKKVYVARGIKYFSGIGVAEFEMESGSLRVGDEILVTGPTTGAVMQTVDEIRVDLKPVEETVKGERFSIKMSEKI
RPSDRLYKMEKVQIEKELR

Sequences:

>Translated_419_residues
MKKKDFEIMAPVGSYESLMAAIQGGADSIYFGIEGLNMRSRSSNNFTTEDLRKIVAICGEHGIKSYLTVNTVIYGEDLPL
MREIIDAAKDAGVSAIIAADVAAMSYANSIGQEVHLSTQLNISNVEALKFYARFADVVVLARELNLKQVHEIYQEIVKQQ
IKGPKGELIRIEMFAHGALCMAVSGKCYLSLHEMNASANRGACMQICRRAYTVHDKDSQIELDVENQYIMSPKDLKTIHF
MNKMMDAGVRVFKLEGRARGPEYVRLVTECYKEAVKAYCEGTFDEEKIAVWDERLRRVFNRGFWDGYYLGQRLGEWSSKY
GSGATRKKVYVARGIKYFSGIGVAEFEMESGSLRVGDEILVTGPTTGAVMQTVDEIRVDLKPVEETVKGERFSIKMSEKI
RPSDRLYKMEKVQIEKELR
>Mature_419_residues
MKKKDFEIMAPVGSYESLMAAIQGGADSIYFGIEGLNMRSRSSNNFTTEDLRKIVAICGEHGIKSYLTVNTVIYGEDLPL
MREIIDAAKDAGVSAIIAADVAAMSYANSIGQEVHLSTQLNISNVEALKFYARFADVVVLARELNLKQVHEIYQEIVKQQ
IKGPKGELIRIEMFAHGALCMAVSGKCYLSLHEMNASANRGACMQICRRAYTVHDKDSQIELDVENQYIMSPKDLKTIHF
MNKMMDAGVRVFKLEGRARGPEYVRLVTECYKEAVKAYCEGTFDEEKIAVWDERLRRVFNRGFWDGYYLGQRLGEWSSKY
GSGATRKKVYVARGIKYFSGIGVAEFEMESGSLRVGDEILVTGPTTGAVMQTVDEIRVDLKPVEETVKGERFSIKMSEKI
RPSDRLYKMEKVQIEKELR

Specific function: Expresses collagenase activity. Hydrolyzes type I collagen. May play a role in virulence [H]

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI87081910, Length=320, Percent_Identity=32.8125, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789548, Length=303, Percent_Identity=29.3729372937294, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788397, Length=374, Percent_Identity=24.5989304812834, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 47233; Mature: 47233

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS01276 PEPTIDASE_U32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKDFEIMAPVGSYESLMAAIQGGADSIYFGIEGLNMRSRSSNNFTTEDLRKIVAICGE
CCCCCCEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
HGIKSYLTVNTVIYGEDLPLMREIIDAAKDAGVSAIIAADVAAMSYANSIGQEVHLSTQL
CCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCEEEEEEEE
NISNVEALKFYARFADVVVLARELNLKQVHEIYQEIVKQQIKGPKGELIRIEMFAHGALC
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEE
MAVSGKCYLSLHEMNASANRGACMQICRRAYTVHDKDSQIELDVENQYIMSPKDLKTIHF
EEECCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEECCHHHHHHHH
MNKMMDAGVRVFKLEGRARGPEYVRLVTECYKEAVKAYCEGTFDEEKIAVWDERLRRVFN
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RGFWDGYYLGQRLGEWSSKYGSGATRKKVYVARGIKYFSGIGVAEFEMESGSLRVGDEIL
CCCCCHHHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHCCCEEEEEECCCCEEECCEEE
VTGPTTGAVMQTVDEIRVDLKPVEETVKGERFSIKMSEKIRPSDRLYKMEKVQIEKELR
EECCCCCHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKDFEIMAPVGSYESLMAAIQGGADSIYFGIEGLNMRSRSSNNFTTEDLRKIVAICGE
CCCCCCEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
HGIKSYLTVNTVIYGEDLPLMREIIDAAKDAGVSAIIAADVAAMSYANSIGQEVHLSTQL
CCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCEEEEEEEE
NISNVEALKFYARFADVVVLARELNLKQVHEIYQEIVKQQIKGPKGELIRIEMFAHGALC
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEE
MAVSGKCYLSLHEMNASANRGACMQICRRAYTVHDKDSQIELDVENQYIMSPKDLKTIHF
EEECCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEECCHHHHHHHH
MNKMMDAGVRVFKLEGRARGPEYVRLVTECYKEAVKAYCEGTFDEEKIAVWDERLRRVFN
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RGFWDGYYLGQRLGEWSSKYGSGATRKKVYVARGIKYFSGIGVAEFEMESGSLRVGDEIL
CCCCCHHHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHCCCEEEEEECCCCEEECCEEE
VTGPTTGAVMQTVDEIRVDLKPVEETVKGERFSIKMSEKIRPSDRLYKMEKVQIEKELR
EECCCCCHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]