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The map label for this gene is pycB [H]

Identifier: 150009281

GI number: 150009281

Start: 3187695

End: 3189620

Strand: Direct

Name: pycB [H]

Synonym: BDI_2683

Alternate gene names: 150009281

Gene position: 3187695-3189620 (Clockwise)

Preceding gene: 150009280

Following gene: 150009282

Centisome position: 66.25

GC content: 50.67

Gene sequence:

>1926_bases
ATGAAAAGAGAAATCAAATTCAGTTTGGTCTTCAGAGACATGTGGCAATCCGCCGGAAAGTATGTCCCGACCGTAGACCA
ACTAACCAGAGTCGCTCCGGCAATTATCGAGATGGGCTGTTTCGCCCGGGTGGAAACGAATGGCGGAGGATTTGAACAAG
TCAACTTATTATTCGGCGAGAATCCTAATAAAGCGGTCCGTGAATGGACAAAACCTTTCCATGCGGCAGGCATTCAGACG
CATATGTTGGACCGTGCGTTAAACGGCCTTCGCATGAGCCCGGTACCCGACGATGTACGTCAGCTATTTTATAAGGTAAA
GAAAGCGCAAGGAACGGATATCGCCCGCACGTTCTGCGGCCTGAACGACGTACGGAATATCGCCCCATCTATTAAATACG
CAAAAGAGGCCGGTATGATCTCACAATGTTCCTTGTGTATCACGCATTCTCCGGTACATACGGTTGAGTATTATACCAAG
ATGGCATTCGAGTTGATCGAACTCGGAGCGGATGAGATTTGTATCAAGGATATGGCCGGTATCGGACGTCCGTACACATT
AGGCCGTATCGTAGCCAATATCAAGGAAAAATATCCAGAGATCCCTATCCAATATCATAGCCATGCCGGTCCCGGCTTTA
ACGTAGCTTCCATCATGGAGGTTTGTAACGCGGGTTGCGACTATATCGACGTAGGTATGGAGCCGCTTTCTTGGGGTACA
GGGCACGCGGACTTGCTGACCGTACAGGCTATGTTGAAGGACGCCGGTTATAAGGTGCCCGAGATCAATATGGAGGCTTA
TATGAAGGTTCGTGCCTTGGTTCAAGAATTCATGGATGATTTCTTGGGATTGTATATCAGCCCGAAGAACCGGTTGATGA
ATTCCTTGCTAATCGGACCGGGACTTCCGGGCGGTATGATGGGTAGCTTGATGGCAGACCTTGAGAAGAACCTAGAGACG
ATCAACAAGAGCAATATAAAGAACAACAAACCGCTGATGTCTCAAGACCAGTTATTGATCAAGTTGTTCGACGAGGTAGC
TTACGTATGGCCACGTGTCGGTTATCCTCCATTGGTAACTCCATTCAGCCAATACGTGAAGAACCTAGCTTTGATGAACG
TCATGCAGATGGAGAAGGGGAAAGCCCGCTGGAGCATGATCGCCGATGATATCTGGGATATGATCCTTGGAAAAGCCGGA
CGACTCCCAGGTCCGTTGGCTCCCGAGATTATCGAGAAGGCGCAAGCCGAGGGACGTAAGTTCTTCGAAGGTAATCCTCA
AGACAATTACCCGGACGCGTTGGATAAGTACCGCAAGTTGATGAACGAGAAACAATGGGAAGTTGGCGAGGACGAGGAAG
AACTCTTCGAATACGCCATGCACCCCGCTCAATACGAGGCATATCGCTCTGGAAAGGCCAAGGTCGAGTTTAAGGCAGAC
GTGGCAAAACGTAAGGCAGAGAAAGCGAACGCCGGTAAACCGACGGTACCCGCCACTCCTGCCGCACCGGCTCCGGCACC
TGCCGCTGCATTAACGATGCCGACGACTCCGCAAGTCATGACCGTAGATGTAAACGGACAGGCTTACCATGTGACCGTCG
CTTTTGGTGATACCAACAGCTCAACTCCGGAAGTTAAACCGACAGTGGCTCCCGCCCCGACAGCCCCAGAGGTAACAAAC
GTTCCCGCAGGCGCTGGGAAAGAGGTGCTTTCTCCGCTGGAAGGTAAATTCTTCCTAGTAAAGAACGCTTCCGATACCCC
GGTGAAGGTGGGCGACGTAGTGAAAGAAGGGGATGTCCTTTGCTACGTGGAAGCCATGAAAACATACAATGCCGTACGTG
CCGAGTTCGGGGGAACGATCACGGCGATCTGCCTGTCTTCCGGTGATGCCGTATCTGAGGATGATGTATTAATGACGATT
CAATAA

Upstream 100 bases:

>100_bases
ACAGGAGACAGCCGCCATCGTATCGGCAATCAGTGCCGTGACCCGTGGACAGGGGAAAGTTATCAAAATTGAGAAAGTAT
AAATAATACATATTAATATC

Downstream 100 bases:

>100_bases
TGAACGAGATATTTCAAAACTTATATGAGATGACTGCGTTCAGCAATATCATTGCTGAACCGCAGTTCTTGATCATGTAT
GCCATAGCGTTCGTATTGCT

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MKREIKFSLVFRDMWQSAGKYVPTVDQLTRVAPAIIEMGCFARVETNGGGFEQVNLLFGENPNKAVREWTKPFHAAGIQT
HMLDRALNGLRMSPVPDDVRQLFYKVKKAQGTDIARTFCGLNDVRNIAPSIKYAKEAGMISQCSLCITHSPVHTVEYYTK
MAFELIELGADEICIKDMAGIGRPYTLGRIVANIKEKYPEIPIQYHSHAGPGFNVASIMEVCNAGCDYIDVGMEPLSWGT
GHADLLTVQAMLKDAGYKVPEINMEAYMKVRALVQEFMDDFLGLYISPKNRLMNSLLIGPGLPGGMMGSLMADLEKNLET
INKSNIKNNKPLMSQDQLLIKLFDEVAYVWPRVGYPPLVTPFSQYVKNLALMNVMQMEKGKARWSMIADDIWDMILGKAG
RLPGPLAPEIIEKAQAEGRKFFEGNPQDNYPDALDKYRKLMNEKQWEVGEDEEELFEYAMHPAQYEAYRSGKAKVEFKAD
VAKRKAEKANAGKPTVPATPAAPAPAPAAALTMPTTPQVMTVDVNGQAYHVTVAFGDTNSSTPEVKPTVAPAPTAPEVTN
VPAGAGKEVLSPLEGKFFLVKNASDTPVKVGDVVKEGDVLCYVEAMKTYNAVRAEFGGTITAICLSSGDAVSEDDVLMTI
Q

Sequences:

>Translated_641_residues
MKREIKFSLVFRDMWQSAGKYVPTVDQLTRVAPAIIEMGCFARVETNGGGFEQVNLLFGENPNKAVREWTKPFHAAGIQT
HMLDRALNGLRMSPVPDDVRQLFYKVKKAQGTDIARTFCGLNDVRNIAPSIKYAKEAGMISQCSLCITHSPVHTVEYYTK
MAFELIELGADEICIKDMAGIGRPYTLGRIVANIKEKYPEIPIQYHSHAGPGFNVASIMEVCNAGCDYIDVGMEPLSWGT
GHADLLTVQAMLKDAGYKVPEINMEAYMKVRALVQEFMDDFLGLYISPKNRLMNSLLIGPGLPGGMMGSLMADLEKNLET
INKSNIKNNKPLMSQDQLLIKLFDEVAYVWPRVGYPPLVTPFSQYVKNLALMNVMQMEKGKARWSMIADDIWDMILGKAG
RLPGPLAPEIIEKAQAEGRKFFEGNPQDNYPDALDKYRKLMNEKQWEVGEDEEELFEYAMHPAQYEAYRSGKAKVEFKAD
VAKRKAEKANAGKPTVPATPAAPAPAPAAALTMPTTPQVMTVDVNGQAYHVTVAFGDTNSSTPEVKPTVAPAPTAPEVTN
VPAGAGKEVLSPLEGKFFLVKNASDTPVKVGDVVKEGDVLCYVEAMKTYNAVRAEFGGTITAICLSSGDAVSEDDVLMTI
Q
>Mature_641_residues
MKREIKFSLVFRDMWQSAGKYVPTVDQLTRVAPAIIEMGCFARVETNGGGFEQVNLLFGENPNKAVREWTKPFHAAGIQT
HMLDRALNGLRMSPVPDDVRQLFYKVKKAQGTDIARTFCGLNDVRNIAPSIKYAKEAGMISQCSLCITHSPVHTVEYYTK
MAFELIELGADEICIKDMAGIGRPYTLGRIVANIKEKYPEIPIQYHSHAGPGFNVASIMEVCNAGCDYIDVGMEPLSWGT
GHADLLTVQAMLKDAGYKVPEINMEAYMKVRALVQEFMDDFLGLYISPKNRLMNSLLIGPGLPGGMMGSLMADLEKNLET
INKSNIKNNKPLMSQDQLLIKLFDEVAYVWPRVGYPPLVTPFSQYVKNLALMNVMQMEKGKARWSMIADDIWDMILGKAG
RLPGPLAPEIIEKAQAEGRKFFEGNPQDNYPDALDKYRKLMNEKQWEVGEDEEELFEYAMHPAQYEAYRSGKAKVEFKAD
VAKRKAEKANAGKPTVPATPAAPAPAPAAALTMPTTPQVMTVDVNGQAYHVTVAFGDTNSSTPEVKPTVAPAPTAPEVTN
VPAGAGKEVLSPLEGKFFLVKNASDTPVKVGDVVKEGDVLCYVEAMKTYNAVRAEFGGTITAICLSSGDAVSEDDVLMTI
Q

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=238, Percent_Identity=27.3109243697479, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI106049295, Length=238, Percent_Identity=27.3109243697479, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI106049292, Length=238, Percent_Identity=27.3109243697479, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562816, Length=669, Percent_Identity=22.7204783258595, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319695, Length=668, Percent_Identity=23.502994011976, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321376, Length=669, Percent_Identity=22.8699551569507, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI281363050, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652224, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652222, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652220, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652218, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652212, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652210, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652214, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19921944, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652216, Length=251, Percent_Identity=31.0756972111554, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 70490; Mature: 70490

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREIKFSLVFRDMWQSAGKYVPTVDQLTRVAPAIIEMGCFARVETNGGGFEQVNLLFGE
CCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECC
NPNKAVREWTKPFHAAGIQTHMLDRALNGLRMSPVPDDVRQLFYKVKKAQGTDIARTFCG
CCCHHHHHHHCHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
LNDVRNIAPSIKYAKEAGMISQCSLCITHSPVHTVEYYTKMAFELIELGADEICIKDMAG
HHHHHHHHHHHHHHHHCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
IGRPYTLGRIVANIKEKYPEIPIQYHSHAGPGFNVASIMEVCNAGCDYIDVGMEPLSWGT
CCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCC
GHADLLTVQAMLKDAGYKVPEINMEAYMKVRALVQEFMDDFLGLYISPKNRLMNSLLIGP
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHCCC
GLPGGMMGSLMADLEKNLETINKSNIKNNKPLMSQDQLLIKLFDEVAYVWPRVGYPPLVT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PFSQYVKNLALMNVMQMEKGKARWSMIADDIWDMILGKAGRLPGPLAPEIIEKAQAEGRK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
FFEGNPQDNYPDALDKYRKLMNEKQWEVGEDEEELFEYAMHPAQYEAYRSGKAKVEFKAD
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCEEEEHHH
VAKRKAEKANAGKPTVPATPAAPAPAPAAALTMPTTPQVMTVDVNGQAYHVTVAFGDTNS
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCEEEEEEEECCCCC
STPEVKPTVAPAPTAPEVTNVPAGAGKEVLSPLEGKFFLVKNASDTPVKVGDVVKEGDVL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCEEHHHHHCCCCEE
CYVEAMKTYNAVRAEFGGTITAICLSSGDAVSEDDVLMTIQ
EEHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKREIKFSLVFRDMWQSAGKYVPTVDQLTRVAPAIIEMGCFARVETNGGGFEQVNLLFGE
CCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECC
NPNKAVREWTKPFHAAGIQTHMLDRALNGLRMSPVPDDVRQLFYKVKKAQGTDIARTFCG
CCCHHHHHHHCHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
LNDVRNIAPSIKYAKEAGMISQCSLCITHSPVHTVEYYTKMAFELIELGADEICIKDMAG
HHHHHHHHHHHHHHHHCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
IGRPYTLGRIVANIKEKYPEIPIQYHSHAGPGFNVASIMEVCNAGCDYIDVGMEPLSWGT
CCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCC
GHADLLTVQAMLKDAGYKVPEINMEAYMKVRALVQEFMDDFLGLYISPKNRLMNSLLIGP
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHCCC
GLPGGMMGSLMADLEKNLETINKSNIKNNKPLMSQDQLLIKLFDEVAYVWPRVGYPPLVT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PFSQYVKNLALMNVMQMEKGKARWSMIADDIWDMILGKAGRLPGPLAPEIIEKAQAEGRK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
FFEGNPQDNYPDALDKYRKLMNEKQWEVGEDEEELFEYAMHPAQYEAYRSGKAKVEFKAD
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCEEEEHHH
VAKRKAEKANAGKPTVPATPAAPAPAPAAALTMPTTPQVMTVDVNGQAYHVTVAFGDTNS
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCEEEEEEEECCCCC
STPEVKPTVAPAPTAPEVTNVPAGAGKEVLSPLEGKFFLVKNASDTPVKVGDVVKEGDVL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCEEHHHHHCCCCEE
CYVEAMKTYNAVRAEFGGTITAICLSSGDAVSEDDVLMTIQ
EEHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]