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The map label for this gene is wecA [H]

Identifier: 150008228

GI number: 150008228

Start: 1866417

End: 1867556

Strand: Reverse

Name: wecA [H]

Synonym: BDI_1594

Alternate gene names: 150008228

Gene position: 1867556-1866417 (Counterclockwise)

Preceding gene: 150008229

Following gene: 150008222

Centisome position: 38.82

GC content: 49.3

Gene sequence:

>1140_bases
ATGCAAATAACATTACTACTACTGTCGGGCTTCCTTTTCTCCGTGCTGTTCGGGATGGTGATCATCCCGAGGATCTTGGT
GATCTCCCACAAGAAACGGTTATATGATGTCCCGGATTCCCGTAAGGTACATACGACGCCGGTCCCCCGTCTGGGCGGCC
TGTCGTTTTTTCCGGTGATCCTGATGTCCATGTTCCTTGTGATAGGCTTCCGCCTGTACTTTTGGGATATGGGCTCATCG
AGCCTGTCGTTCAACATGCTCTATGAGTATCTCTTTCTGTTCGTGGGCATGACGTTACTCTATCTAGTGGGTGTCTGCGA
CGATCTAGTGGGTGTTGGCTACCGTTACAAGTTCGCCGTCCAGATCGTGTCGGCGTTATTATTAGTACTGTCCGGTAATT
GGTTCGGTTCCTTGGGAGGCTTGTTCGGTGTGTACTCGGTTCCCGCTTGGGTGGGGATCCCCGTCACGGTCTTTATCGTG
GTGTATATCACGAACGCCATTAACTTGATAGACGGTATCGATGGCTTGGCCTCCGGTTTGTGCAGTATCGCCCTGTCGGT
CTTGAGCGTGATCTTCTTTATCCGCATGCAGTATGTGTACGCCTTGTTGGCCATCTGTACGCTGGGAGTCCTCATGCCTT
TTTGGTGTTACAACGTCTTTGGCAACGCAAACCGTGGCCATAAGCTCTTTATGGGTGATGCGGGTAGTCTTACGCTGGGC
TACGTGATCAGTTTCCTGATCATCCATCTGTGCGTGTCGAACCAAGTCTCACCGGAGTTACCAAATCCCTATATGGTGAT
CGCTTTCTCCACGGTCTTGGTCCCTTTACTCGATGTGATCCGTGTGGTGCTTCATCGCCTTCGTAATCATCGTAACCCGT
TCTTGCCCGATAAGAATCATTTCCATCACAAGCTGCTTCGTACGGGTATGCGTGTACGGATGGTGATGGTGACGATTCTG
TGCGTATCTGTTTTTTTCATCGTATTGAATGTCTTATTGGCCATGAAGGTAAACGCCACCTACCTCTTGCTCTTGAACTT
GGTTTTATGGTCTATCCTTCACTTGACGATCAACCGCTTCATAACGAGGCATCATAAAGAGCAAGAGCTGAAAGAGATAG
GGGGTATTTTTACAAAGTAA

Upstream 100 bases:

>100_bases
ACGGCAAGAAATACGGCTACGGCAAGGGATACGGTTATGGCTACGGCTATGGTTACGGTTTCGAGGCCGGTGACAAGAAG
AAATAACTAATACGCAAATC

Downstream 100 bases:

>100_bases
GTCGTGAAACCGGACAATCTAGCGAGTGAATGACGAGCCGGTATCTATTTTATAAGACGAGATACTCCTCCCTTGCGTCA
AAGCAAGGGCAAGCCTTGCG

Product: putative LPS biosynthesis glycosyltransferase

Products: UMP; Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

Alternate protein names: UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-Phosphate GlcNAc-1-phosphate transferase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MQITLLLLSGFLFSVLFGMVIIPRILVISHKKRLYDVPDSRKVHTTPVPRLGGLSFFPVILMSMFLVIGFRLYFWDMGSS
SLSFNMLYEYLFLFVGMTLLYLVGVCDDLVGVGYRYKFAVQIVSALLLVLSGNWFGSLGGLFGVYSVPAWVGIPVTVFIV
VYITNAINLIDGIDGLASGLCSIALSVLSVIFFIRMQYVYALLAICTLGVLMPFWCYNVFGNANRGHKLFMGDAGSLTLG
YVISFLIIHLCVSNQVSPELPNPYMVIAFSTVLVPLLDVIRVVLHRLRNHRNPFLPDKNHFHHKLLRTGMRVRMVMVTIL
CVSVFFIVLNVLLAMKVNATYLLLLNLVLWSILHLTINRFITRHHKEQELKEIGGIFTK

Sequences:

>Translated_379_residues
MQITLLLLSGFLFSVLFGMVIIPRILVISHKKRLYDVPDSRKVHTTPVPRLGGLSFFPVILMSMFLVIGFRLYFWDMGSS
SLSFNMLYEYLFLFVGMTLLYLVGVCDDLVGVGYRYKFAVQIVSALLLVLSGNWFGSLGGLFGVYSVPAWVGIPVTVFIV
VYITNAINLIDGIDGLASGLCSIALSVLSVIFFIRMQYVYALLAICTLGVLMPFWCYNVFGNANRGHKLFMGDAGSLTLG
YVISFLIIHLCVSNQVSPELPNPYMVIAFSTVLVPLLDVIRVVLHRLRNHRNPFLPDKNHFHHKLLRTGMRVRMVMVTIL
CVSVFFIVLNVLLAMKVNATYLLLLNLVLWSILHLTINRFITRHHKEQELKEIGGIFTK
>Mature_379_residues
MQITLLLLSGFLFSVLFGMVIIPRILVISHKKRLYDVPDSRKVHTTPVPRLGGLSFFPVILMSMFLVIGFRLYFWDMGSS
SLSFNMLYEYLFLFVGMTLLYLVGVCDDLVGVGYRYKFAVQIVSALLLVLSGNWFGSLGGLFGVYSVPAWVGIPVTVFIV
VYITNAINLIDGIDGLASGLCSIALSVLSVIFFIRMQYVYALLAICTLGVLMPFWCYNVFGNANRGHKLFMGDAGSLTLG
YVISFLIIHLCVSNQVSPELPNPYMVIAFSTVLVPLLDVIRVVLHRLRNHRNPFLPDKNHFHHKLLRTGMRVRMVMVTIL
CVSVFFIVLNVLLAMKVNATYLLLLNLVLWSILHLTINRFITRHHKEQELKEIGGIFTK

Specific function: Catalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP- C55) [H]

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family. WecA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790218, Length=294, Percent_Identity=27.2108843537415, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012750
- InterPro:   IPR000715
- InterPro:   IPR018480 [H]

Pfam domain/function: PF00953 Glycos_transf_4 [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 42737; Mature: 42737

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS01348 MRAY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITLLLLSGFLFSVLFGMVIIPRILVISHKKRLYDVPDSRKVHTTPVPRLGGLSFFPVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHHH
LMSMFLVIGFRLYFWDMGSSSLSFNMLYEYLFLFVGMTLLYLVGVCDDLVGVGYRYKFAV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QIVSALLLVLSGNWFGSLGGLFGVYSVPAWVGIPVTVFIVVYITNAINLIDGIDGLASGL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSIALSVLSVIFFIRMQYVYALLAICTLGVLMPFWCYNVFGNANRGHKLFMGDAGSLTLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHH
YVISFLIIHLCVSNQVSPELPNPYMVIAFSTVLVPLLDVIRVVLHRLRNHRNPFLPDKNH
HHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
FHHKLLRTGMRVRMVMVTILCVSVFFIVLNVLLAMKVNATYLLLLNLVLWSILHLTINRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ITRHHKEQELKEIGGIFTK
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQITLLLLSGFLFSVLFGMVIIPRILVISHKKRLYDVPDSRKVHTTPVPRLGGLSFFPVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHHH
LMSMFLVIGFRLYFWDMGSSSLSFNMLYEYLFLFVGMTLLYLVGVCDDLVGVGYRYKFAV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QIVSALLLVLSGNWFGSLGGLFGVYSVPAWVGIPVTVFIVVYITNAINLIDGIDGLASGL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSIALSVLSVIFFIRMQYVYALLAICTLGVLMPFWCYNVFGNANRGHKLFMGDAGSLTLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHH
YVISFLIIHLCVSNQVSPELPNPYMVIAFSTVLVPLLDVIRVVLHRLRNHRNPFLPDKNH
HHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
FHHKLLRTGMRVRMVMVTILCVSVFFIVLNVLLAMKVNATYLLLLNLVLWSILHLTINRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ITRHHKEQELKEIGGIFTK
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-Acetyl-D-Glucosamine; Undecaprenyl Phosphate [C]

Specific reaction: UDP-N-Acetyl-D-Glucosamine + Undecaprenyl Phosphate = UMP + Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]