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The map label for this gene is aspS

Identifier: 150008039

GI number: 150008039

Start: 1629936

End: 1631693

Strand: Reverse

Name: aspS

Synonym: BDI_1402

Alternate gene names: 150008039

Gene position: 1631693-1629936 (Counterclockwise)

Preceding gene: 150008040

Following gene: 150008038

Centisome position: 33.91

GC content: 48.41

Gene sequence:

>1758_bases
ATGTATAGAACAAGAACTTGTGGCGACTTGCGCCTTGCGGATGAAGGTCTGGTGGTTACACTGGCCGGTTGGGTGCAGAA
AACCCGCAAAATGGGAGGAATGACATTTGTCGATATCCGCGACCGTTATGGTATTACTCAGTTGGTATTTAATCAGGAGG
TTGACGCAGCTCTTTGCGAAAAGGCGAATAAACTGGGCCGTGAATTCGTGATTCAGGTTACGGGTACGGTTCGTGAGCGT
TCCAGCAAGAATGCGCATATCCCGACAGGTGATATTGAGTTGATCGTTTCTGAATTAAATGTCCTGAACACTGCGCTTAC
CCCTCCGTTCACGATCGAGGAGGAGACAGATGGCGGTGATGACTTGCGTATGAAATATCGTTATCTGGACTTGCGTCGTG
CTTGCGTTCGTAAGAATCTGGAACTTCGTCATAGAATGGCGTTCGAGGTTCGCCGCTATTTGGATGAGCAAGGTTTCTTG
GAGGTAGAGACTCCGGTTCTTGTAAATTCTACCCCGGAGGGTGCCCGTGACTTCGTGGTTCCTTCCCGTATGAACCCGGG
ACAGTTCTACGCTTTGCCTCAGTCTCCACAGACCTTGAAGCAATTGTTGATGGTTTCCGGTTTCGACCGTTATTTCCAGA
TCGTAAAATGTTTCCGTGACGAGGATTTGCGTGCGGATCGCCAGCCGGAGTTCACGCAGATCGACTGTGAGATGAGCTTC
GTGGAGCAAGAGGATGTCTTGAATATGTTCGAGGGCATGGCGAAACATTTGTTTAAGGTAATCCGTGGTGTCGAGTTCAA
GGAGTCGTTTATGCGTATGACTTGGCAAGATGCTATGAAACAATATGGTAGCGATAAACCGGATTTGCGTTTCGGCATGA
AGTTCGTCGAGTTGATGGACATCATGAAAGGCCATGGTTTCTCCGTATTCGATAACGCTGCTTACATAGGGGGTATCTGT
GCGGAAGGTGCTGCAAGCTATACCCGTAAACAACTGGATGCGCTGACTGAATTCGTGAAACGTCCGCAGGTAGGTGCTAA
AGGTTTGGTTTACGCCCGTGTGGAAGCGGATGGTAACGTGAAATCAAGCGTAGATAAATTCTATAGCCAAGAGGTTCTTC
AAGAAATGAAGAATGCGTTCGGCGCTAAGCCGGGTGATTTGATTTTGATCCTTTCCGGCGACGATGCGATGAAGACTCGT
AAGCAACTTTGCGAGTTGCGTTTAGAGATGGGTAATCAATTAGGCCTTCGTGACAAGGATAAGTTCGTTTGCTTGTGGGT
AATCGACTTCCCGCTGTTCGAATGGAACGAGGACGATCAACGTTTCTACGCTATGCACCATCCGTTTACCTCTCCGAATC
CGGATGATATACCGTTGTTGGACACGGATCCGGGCGCTGTTCGTGCGAATGCGTATGATATGGTTATCAATGGCGTTGAA
GTGGGTGGTGGTTCTATCCGTATCCACGACAGCCAATTACAGGATAAGATGTTCAAGCTGTTAGGATTCACCGAAGAAAG
AGCGCAAGAGCAATTCGGTTTCTTGATGAATGCGTTCAAGTACGGAGCGCCTCCTCATGGAGGTTTGGCTTATGGCTTGG
ATCGTTTCGTGTCTTTGTTCGCCGGTTTGGATTCTATCCGCGACTGTATCGCTTTCCCGAAGAACAATTCTGGACGAGAT
GTTATGCTCGATGCTCCGGGTGTCTTGGATCCGGCCCAGTTGGACGAACTGAATTTGATCGTTGATATCAAGAAATAA

Upstream 100 bases:

>100_bases
AAAGAATAATTATTAAGATTTATAGTATTATCTTTGTGGTTGCGGTTTGTAATAAACCGAGGTATAAAGATTGGTAAATC
TAGCAAATTAAATAATAAAG

Downstream 100 bases:

>100_bases
TCCAATCTGAAATTCCATTCGAATGGGAATCTTATTCCATCTGAATGGAATTTTCTTTTCTTCCGAAAGGAATTACTTTT
TCTCCCGAACATAAATCAAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MYRTRTCGDLRLADEGLVVTLAGWVQKTRKMGGMTFVDIRDRYGITQLVFNQEVDAALCEKANKLGREFVIQVTGTVRER
SSKNAHIPTGDIELIVSELNVLNTALTPPFTIEEETDGGDDLRMKYRYLDLRRACVRKNLELRHRMAFEVRRYLDEQGFL
EVETPVLVNSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
VEQEDVLNMFEGMAKHLFKVIRGVEFKESFMRMTWQDAMKQYGSDKPDLRFGMKFVELMDIMKGHGFSVFDNAAYIGGIC
AEGAASYTRKQLDALTEFVKRPQVGAKGLVYARVEADGNVKSSVDKFYSQEVLQEMKNAFGAKPGDLILILSGDDAMKTR
KQLCELRLEMGNQLGLRDKDKFVCLWVIDFPLFEWNEDDQRFYAMHHPFTSPNPDDIPLLDTDPGAVRANAYDMVINGVE
VGGGSIRIHDSQLQDKMFKLLGFTEERAQEQFGFLMNAFKYGAPPHGGLAYGLDRFVSLFAGLDSIRDCIAFPKNNSGRD
VMLDAPGVLDPAQLDELNLIVDIKK

Sequences:

>Translated_585_residues
MYRTRTCGDLRLADEGLVVTLAGWVQKTRKMGGMTFVDIRDRYGITQLVFNQEVDAALCEKANKLGREFVIQVTGTVRER
SSKNAHIPTGDIELIVSELNVLNTALTPPFTIEEETDGGDDLRMKYRYLDLRRACVRKNLELRHRMAFEVRRYLDEQGFL
EVETPVLVNSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
VEQEDVLNMFEGMAKHLFKVIRGVEFKESFMRMTWQDAMKQYGSDKPDLRFGMKFVELMDIMKGHGFSVFDNAAYIGGIC
AEGAASYTRKQLDALTEFVKRPQVGAKGLVYARVEADGNVKSSVDKFYSQEVLQEMKNAFGAKPGDLILILSGDDAMKTR
KQLCELRLEMGNQLGLRDKDKFVCLWVIDFPLFEWNEDDQRFYAMHHPFTSPNPDDIPLLDTDPGAVRANAYDMVINGVE
VGGGSIRIHDSQLQDKMFKLLGFTEERAQEQFGFLMNAFKYGAPPHGGLAYGLDRFVSLFAGLDSIRDCIAFPKNNSGRD
VMLDAPGVLDPAQLDELNLIVDIKK
>Mature_585_residues
MYRTRTCGDLRLADEGLVVTLAGWVQKTRKMGGMTFVDIRDRYGITQLVFNQEVDAALCEKANKLGREFVIQVTGTVRER
SSKNAHIPTGDIELIVSELNVLNTALTPPFTIEEETDGGDDLRMKYRYLDLRRACVRKNLELRHRMAFEVRRYLDEQGFL
EVETPVLVNSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
VEQEDVLNMFEGMAKHLFKVIRGVEFKESFMRMTWQDAMKQYGSDKPDLRFGMKFVELMDIMKGHGFSVFDNAAYIGGIC
AEGAASYTRKQLDALTEFVKRPQVGAKGLVYARVEADGNVKSSVDKFYSQEVLQEMKNAFGAKPGDLILILSGDDAMKTR
KQLCELRLEMGNQLGLRDKDKFVCLWVIDFPLFEWNEDDQRFYAMHHPFTSPNPDDIPLLDTDPGAVRANAYDMVINGVE
VGGGSIRIHDSQLQDKMFKLLGFTEERAQEQFGFLMNAFKYGAPPHGGLAYGLDRFVSLFAGLDSIRDCIAFPKNNSGRD
VMLDAPGVLDPAQLDELNLIVDIKK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=40.3333333333333, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI45439306, Length=322, Percent_Identity=23.2919254658385, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI5031815, Length=256, Percent_Identity=27.734375, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI194272210, Length=256, Percent_Identity=27.734375, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788173, Length=594, Percent_Identity=47.4747474747475, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI1790571, Length=281, Percent_Identity=26.6903914590747, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789256, Length=279, Percent_Identity=27.5985663082437, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=587, Percent_Identity=36.6269165247019, Blast_Score=338, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI71994340, Length=300, Percent_Identity=26.3333333333333, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=300, Percent_Identity=26.3333333333333, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=300, Percent_Identity=26.3333333333333, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=323, Percent_Identity=21.9814241486068, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=642, Percent_Identity=32.2429906542056, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6321807, Length=290, Percent_Identity=26.551724137931, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=244, Percent_Identity=26.6393442622951, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=253, Percent_Identity=25.6916996047431, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24584738, Length=594, Percent_Identity=36.026936026936, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI17136276, Length=269, Percent_Identity=26.7657992565056, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640851, Length=261, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24640849, Length=261, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_PARD8 (A6LBU6)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302782.1
- ProteinModelPortal:   A6LBU6
- SMR:   A6LBU6
- STRING:   A6LBU6
- GeneID:   5306555
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1402
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   PDIS435591:BDI_1402-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66429; Mature: 66429

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRTRTCGDLRLADEGLVVTLAGWVQKTRKMGGMTFVDIRDRYGITQLVFNQEVDAALCE
CCCCCCCCCCEECCCCEEEEHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCHHHHHHH
KANKLGREFVIQVTGTVRERSSKNAHIPTGDIELIVSELNVLNTALTPPFTIEEETDGGD
HHHHCCCEEEEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCC
DLRMKYRYLDLRRACVRKNLELRHRMAFEVRRYLDEQGFLEVETPVLVNSTPEGARDFVV
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCCCC
PSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
CCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHH
VEQEDVLNMFEGMAKHLFKVIRGVEFKESFMRMTWQDAMKQYGSDKPDLRFGMKFVELMD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IMKGHGFSVFDNAAYIGGICAEGAASYTRKQLDALTEFVKRPQVGAKGLVYARVEADGNV
HHHCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
KSSVDKFYSQEVLQEMKNAFGAKPGDLILILSGDDAMKTRKQLCELRLEMGNQLGLRDKD
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCC
KFVCLWVIDFPLFEWNEDDQRFYAMHHPFTSPNPDDIPLLDTDPGAVRANAYDMVINGVE
CEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCEECCCEEEEEEEEE
VGGGSIRIHDSQLQDKMFKLLGFTEERAQEQFGFLMNAFKYGAPPHGGLAYGLDRFVSLF
ECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
AGLDSIRDCIAFPKNNSGRDVMLDAPGVLDPAQLDELNLIVDIKK
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHCCEEEEEEECC
>Mature Secondary Structure
MYRTRTCGDLRLADEGLVVTLAGWVQKTRKMGGMTFVDIRDRYGITQLVFNQEVDAALCE
CCCCCCCCCCEECCCCEEEEHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCHHHHHHH
KANKLGREFVIQVTGTVRERSSKNAHIPTGDIELIVSELNVLNTALTPPFTIEEETDGGD
HHHHCCCEEEEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCC
DLRMKYRYLDLRRACVRKNLELRHRMAFEVRRYLDEQGFLEVETPVLVNSTPEGARDFVV
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCCCC
PSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
CCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHH
VEQEDVLNMFEGMAKHLFKVIRGVEFKESFMRMTWQDAMKQYGSDKPDLRFGMKFVELMD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IMKGHGFSVFDNAAYIGGICAEGAASYTRKQLDALTEFVKRPQVGAKGLVYARVEADGNV
HHHCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
KSSVDKFYSQEVLQEMKNAFGAKPGDLILILSGDDAMKTRKQLCELRLEMGNQLGLRDKD
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCC
KFVCLWVIDFPLFEWNEDDQRFYAMHHPFTSPNPDDIPLLDTDPGAVRANAYDMVINGVE
CEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCEECCCEEEEEEEEE
VGGGSIRIHDSQLQDKMFKLLGFTEERAQEQFGFLMNAFKYGAPPHGGLAYGLDRFVSLF
ECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
AGLDSIRDCIAFPKNNSGRDVMLDAPGVLDPAQLDELNLIVDIKK
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA