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The map label for this gene is mscS [H]

Identifier: 150005718

GI number: 150005718

Start: 4037109

End: 4037954

Strand: Direct

Name: mscS [H]

Synonym: BVU_3211

Alternate gene names: 150005718

Gene position: 4037109-4037954 (Clockwise)

Preceding gene: 150005715

Following gene: 150005719

Centisome position: 78.19

GC content: 38.3

Gene sequence:

>846_bases
ATGAATTTATTATTATTGAATGCAGCCTCAGGCAGTAAACTTGAGCAAGCACTGGAGAAACTGGTAGATTTTGGAATGGA
CGCTGGTAAGGATATATTTGTCGCCATTCTGATATATTCAGTCGGTCGTTTTATCATTAAGCAGATTAGTGTATTAATAG
CCAAAGTTCTTGAAAAACGGAAGATAGAAACCAGTGTACAGACTTTCTTGAAAAGTTTAGTGAAAATTTTATTGAACATG
GTACTTGCTTTTGCCATAATCAGCAAGTTGGGAGTGGAAACGACCAGTTTTGCTGCACTTTTGGCGTCAGCCGGTGTGGC
TGTAGGTATGGCACTTTCCGGCAATCTTTCCAATTTCGCAGGTGGTTTGATTATACTTGTTTTTAAACCGTTTAAAGTAG
GCGATTATATAGATGGACCTGGAGTAAGTGGTACAATAAAAGAGATACAGATTTTCCATACCATACTTTCCACTCTTGAT
AATCGGATGATATACGTTCCCAACGGGATGCTCAGTGGAAATGCTGTGATTAATTACAGCAAACAGGAAATGCGCCGTGT
TGAATGGGTATTCGGAGTAGAATATGGAGAGGATGTACAAAGGGTAAGAACCGTATTGCAACGTATTATTTATGCGGACA
AACGTATTTTGGATACTCCTGCCCCTATCATTGTTTTAGGCTCATTAAGTGCGAGTAGCGTTGACATTACTGTCCGTGTT
TGGGTAAAAACAGCTGATTACTGGAATGTTTTATATGATATTAATGAGATTGTTTATACGACATTTAATAAAGAAGGAAT
CGGATTCCCTTTCCCACAACTTACTGTGCATCAATCTTCAAAATAA

Upstream 100 bases:

>100_bases
ATTTATTGGAACATTGCGAAACTATACTGTCAGGATGTGCTGGCATATTTGCAATATGATCGTGACAAGCTGATTAATTT
TTATTTGTTAAACGAAAAAA

Downstream 100 bases:

>100_bases
TAGAAACATATTCAACTTCTTAAATAAATGATCATGAAAAAATTAATTTGTTTTTTTACGATTTTCGTATTTTGGGGAAT
CACTGATAGTATAGCTCAGG

Product: putative transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNLLLLNAASGSKLEQALEKLVDFGMDAGKDIFVAILIYSVGRFIIKQISVLIAKVLEKRKIETSVQTFLKSLVKILLNM
VLAFAIISKLGVETTSFAALLASAGVAVGMALSGNLSNFAGGLIILVFKPFKVGDYIDGPGVSGTIKEIQIFHTILSTLD
NRMIYVPNGMLSGNAVINYSKQEMRRVEWVFGVEYGEDVQRVRTVLQRIIYADKRILDTPAPIIVLGSLSASSVDITVRV
WVKTADYWNVLYDINEIVYTTFNKEGIGFPFPQLTVHQSSK

Sequences:

>Translated_281_residues
MNLLLLNAASGSKLEQALEKLVDFGMDAGKDIFVAILIYSVGRFIIKQISVLIAKVLEKRKIETSVQTFLKSLVKILLNM
VLAFAIISKLGVETTSFAALLASAGVAVGMALSGNLSNFAGGLIILVFKPFKVGDYIDGPGVSGTIKEIQIFHTILSTLD
NRMIYVPNGMLSGNAVINYSKQEMRRVEWVFGVEYGEDVQRVRTVLQRIIYADKRILDTPAPIIVLGSLSASSVDITVRV
WVKTADYWNVLYDINEIVYTTFNKEGIGFPFPQLTVHQSSK
>Mature_281_residues
MNLLLLNAASGSKLEQALEKLVDFGMDAGKDIFVAILIYSVGRFIIKQISVLIAKVLEKRKIETSVQTFLKSLVKILLNM
VLAFAIISKLGVETTSFAALLASAGVAVGMALSGNLSNFAGGLIILVFKPFKVGDYIDGPGVSGTIKEIQIFHTILSTLD
NRMIYVPNGMLSGNAVINYSKQEMRRVEWVFGVEYGEDVQRVRTVLQRIIYADKRILDTPAPIIVLGSLSASSVDITVRV
WVKTADYWNVLYDINEIVYTTFNKEGIGFPFPQLTVHQSSK

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=247, Percent_Identity=38.4615384615385, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1786670, Length=197, Percent_Identity=28.9340101522843, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI2367355, Length=268, Percent_Identity=23.8805970149254, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787591, Length=241, Percent_Identity=26.9709543568465, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30928; Mature: 30928

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLLLNAASGSKLEQALEKLVDFGMDAGKDIFVAILIYSVGRFIIKQISVLIAKVLEKR
CCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIETSVQTFLKSLVKILLNMVLAFAIISKLGVETTSFAALLASAGVAVGMALSGNLSNFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCCHHHC
GGLIILVFKPFKVGDYIDGPGVSGTIKEIQIFHTILSTLDNRMIYVPNGMLSGNAVINYS
CCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECC
KQEMRRVEWVFGVEYGEDVQRVRTVLQRIIYADKRILDTPAPIIVLGSLSASSVDITVRV
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEECCCCCCEEEEEEE
WVKTADYWNVLYDINEIVYTTFNKEGIGFPFPQLTVHQSSK
EEEECHHHHHHHHHHHHHEEEECCCCCCCCCCCEEECCCCC
>Mature Secondary Structure
MNLLLLNAASGSKLEQALEKLVDFGMDAGKDIFVAILIYSVGRFIIKQISVLIAKVLEKR
CCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIETSVQTFLKSLVKILLNMVLAFAIISKLGVETTSFAALLASAGVAVGMALSGNLSNFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCCHHHC
GGLIILVFKPFKVGDYIDGPGVSGTIKEIQIFHTILSTLDNRMIYVPNGMLSGNAVINYS
CCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECC
KQEMRRVEWVFGVEYGEDVQRVRTVLQRIIYADKRILDTPAPIIVLGSLSASSVDITVRV
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEECCCCCCEEEEEEE
WVKTADYWNVLYDINEIVYTTFNKEGIGFPFPQLTVHQSSK
EEEECHHHHHHHHHHHHHEEEECCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]