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The map label for this gene is mhpE [C]

Identifier: 150005191

GI number: 150005191

Start: 3378964

End: 3380496

Strand: Reverse

Name: mhpE [C]

Synonym: BVU_2661

Alternate gene names: 150005191

Gene position: 3380496-3378964 (Counterclockwise)

Preceding gene: 150005192

Following gene: 150005190

Centisome position: 65.47

GC content: 32.62

Gene sequence:

>1533_bases
ATGAAAATATTAGATTGTACTCTTCGTGATGGTGGATATTACACCAATTGGGATTTTAACTCAAAAATTGTCGACGCTTA
TATACTAGCCATGAACGAACTTCCTATTGACTATTTGGAAGTTGGTTATAGAAATAAACCAAGTAAAGAGTATATGGGAA
AATTTGGATATACTCCTGTATCTGTTTTAAAACATTTAAGGAATATATCCACAAAAAAAATAGCAATAATGCTAAATGAA
AAAAACACGACCCCTGAAGATCTCAATCATTTACTTCTGCCCATAATAGGATTGGTAGACATGATACGTATTGCCATTGA
TCCTCAAAATATAGACCGTGCAATAGTTCTAGCAAAAGCTATAAAAACAATGGGATTTGAAGTTGGTTTTAATGTAATGT
ATATGTCAAAATGGGCAGAAATGAATGGATTTTTGTCTAAATTGAAGGCTATTGACAAAATTGCTGATTTATTTTGTATG
GTCGATTCTTTTGGAGGTATCACACCTAAAGAAGTGAAAAATCTACTTAAAGAAGTACGTAAATATACTCATGTTCCCGT
TGGATTCCACGGACACGACAACCTACAATTAGGATTGATTAACTCTATTACCGCAATAGATGATGGCATAGACTTTATAG
ACGCAACCATTACAGGTATGGGACGTGGTGCAGGTAACCTAAAGATGGAATTATTACTTACATATCTTAACAAACATCAT
GGATTAAATGTTGATTTCAATGTGCTTGGAAATATAATAACCACTTTTACTCCATTACTTGAAAAATATCAATGGGGAAC
AAATCTGCCATACATGCTTTCTGGAGCAAATAATATTCCACAAAAAGAAGTTATGGATTGGGTAACAAATCGTGTTTATT
CATTTAATAGTATTATACGAGCGCTTGATAACAGAAAAAATAAAATGGAGGATAATGCCAAATATCCTTTACTTAATATA
AAGAACAAATTTGATAGAGTAGTAATTATTGGTGGTGGATTTAATGCGATAGAACATAAAGAAGCTATCAAGTCTTTCAT
TGAAACACATACTAATACAGCTATTATTTTTGCCACAGCTCGATATGCACGAGAATATCTTGATGTAAATGCACCTCATT
TTTATTGTCTTGTTGGTAACGAAGGGCATAGATTGACACATAATATAAATCCCCAAAATCTTTCAGGAATATGTGTATTA
CCGCCATATCCGCGTCCTATGGGTACAGAAGTTCCCGAGTATGCCAAAAATGTTACATTTGAACTCGAAAACATAACTTT
CATTGATCAATACAAAGATTCTGTAACAACAATAGCATTACAATTAGCAATTTTACTTACTGATCAAGACATTTATCTTG
TTGGCTATGACGGATATCCGGGGAATGTACTTTCTGAAAAAGAAATGGCACTAACAAATGAAAACAGAACTATATTTGCT
ACATATACCACCATTAGTGGAAAAATACTAAAATCACTTACTCCGTCTATATACAAAGAAATTGAAGTCGTGTCTGTATA
CCAGTTTATATAA

Upstream 100 bases:

>100_bases
TTCAATACAGAAATGATATAATATTAAAAGGAATTGATAATGTAAAAAAATACAGTATTGAAACTGTAGCTAAACAATAT
TTTGAATTATATAAAACATT

Downstream 100 bases:

>100_bases
TCCAACACTTGTACTATACAAATTCCATATTATACAAAAAATTATATACAAATGAATAATAAAATATCTTTTTTCCTTCC
AACCCGCAAAGGAAGTGAAC

Product: putative aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKILDCTLRDGGYYTNWDFNSKIVDAYILAMNELPIDYLEVGYRNKPSKEYMGKFGYTPVSVLKHLRNISTKKIAIMLNE
KNTTPEDLNHLLLPIIGLVDMIRIAIDPQNIDRAIVLAKAIKTMGFEVGFNVMYMSKWAEMNGFLSKLKAIDKIADLFCM
VDSFGGITPKEVKNLLKEVRKYTHVPVGFHGHDNLQLGLINSITAIDDGIDFIDATITGMGRGAGNLKMELLLTYLNKHH
GLNVDFNVLGNIITTFTPLLEKYQWGTNLPYMLSGANNIPQKEVMDWVTNRVYSFNSIIRALDNRKNKMEDNAKYPLLNI
KNKFDRVVIIGGGFNAIEHKEAIKSFIETHTNTAIIFATARYAREYLDVNAPHFYCLVGNEGHRLTHNINPQNLSGICVL
PPYPRPMGTEVPEYAKNVTFELENITFIDQYKDSVTTIALQLAILLTDQDIYLVGYDGYPGNVLSEKEMALTNENRTIFA
TYTTISGKILKSLTPSIYKEIEVVSVYQFI

Sequences:

>Translated_510_residues
MKILDCTLRDGGYYTNWDFNSKIVDAYILAMNELPIDYLEVGYRNKPSKEYMGKFGYTPVSVLKHLRNISTKKIAIMLNE
KNTTPEDLNHLLLPIIGLVDMIRIAIDPQNIDRAIVLAKAIKTMGFEVGFNVMYMSKWAEMNGFLSKLKAIDKIADLFCM
VDSFGGITPKEVKNLLKEVRKYTHVPVGFHGHDNLQLGLINSITAIDDGIDFIDATITGMGRGAGNLKMELLLTYLNKHH
GLNVDFNVLGNIITTFTPLLEKYQWGTNLPYMLSGANNIPQKEVMDWVTNRVYSFNSIIRALDNRKNKMEDNAKYPLLNI
KNKFDRVVIIGGGFNAIEHKEAIKSFIETHTNTAIIFATARYAREYLDVNAPHFYCLVGNEGHRLTHNINPQNLSGICVL
PPYPRPMGTEVPEYAKNVTFELENITFIDQYKDSVTTIALQLAILLTDQDIYLVGYDGYPGNVLSEKEMALTNENRTIFA
TYTTISGKILKSLTPSIYKEIEVVSVYQFI
>Mature_510_residues
MKILDCTLRDGGYYTNWDFNSKIVDAYILAMNELPIDYLEVGYRNKPSKEYMGKFGYTPVSVLKHLRNISTKKIAIMLNE
KNTTPEDLNHLLLPIIGLVDMIRIAIDPQNIDRAIVLAKAIKTMGFEVGFNVMYMSKWAEMNGFLSKLKAIDKIADLFCM
VDSFGGITPKEVKNLLKEVRKYTHVPVGFHGHDNLQLGLINSITAIDDGIDFIDATITGMGRGAGNLKMELLLTYLNKHH
GLNVDFNVLGNIITTFTPLLEKYQWGTNLPYMLSGANNIPQKEVMDWVTNRVYSFNSIIRALDNRKNKMEDNAKYPLLNI
KNKFDRVVIIGGGFNAIEHKEAIKSFIETHTNTAIIFATARYAREYLDVNAPHFYCLVGNEGHRLTHNINPQNLSGICVL
PPYPRPMGTEVPEYAKNVTFELENITFIDQYKDSVTTIALQLAILLTDQDIYLVGYDGYPGNVLSEKEMALTNENRTIFA
TYTTISGKILKSLTPSIYKEIEVVSVYQFI

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 57704; Mature: 57704

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILDCTLRDGGYYTNWDFNSKIVDAYILAMNELPIDYLEVGYRNKPSKEYMGKFGYTPV
CCEEEEEECCCCEEECCCCCCHHHHHHEEHHCCCCHHHHHCCCCCCCCHHHHHCCCCCHH
SVLKHLRNISTKKIAIMLNEKNTTPEDLNHLLLPIIGLVDMIRIAIDPQNIDRAIVLAKA
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHH
IKTMGFEVGFNVMYMSKWAEMNGFLSKLKAIDKIADLFCMVDSFGGITPKEVKNLLKEVR
HHHCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KYTHVPVGFHGHDNLQLGLINSITAIDDGIDFIDATITGMGRGAGNLKMELLLTYLNKHH
HHHCCCCEECCCCCEEEEECHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
GLNVDFNVLGNIITTFTPLLEKYQWGTNLPYMLSGANNIPQKEVMDWVTNRVYSFNSIIR
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ALDNRKNKMEDNAKYPLLNIKNKFDRVVIIGGGFNAIEHKEAIKSFIETHTNTAIIFATA
HHHCCHHHCCCCCCCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEH
RYAREYLDVNAPHFYCLVGNEGHRLTHNINPQNLSGICVLPPYPRPMGTEVPEYAKNVTF
HHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCHHHHCCCEE
ELENITFIDQYKDSVTTIALQLAILLTDQDIYLVGYDGYPGNVLSEKEMALTNENRTIFA
EECCEEEEHHHHHHHHHHHHEEEEEEECCEEEEEEECCCCCCCCCCCHHEEECCCCEEEE
TYTTISGKILKSLTPSIYKEIEVVSVYQFI
EEEECCHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILDCTLRDGGYYTNWDFNSKIVDAYILAMNELPIDYLEVGYRNKPSKEYMGKFGYTPV
CCEEEEEECCCCEEECCCCCCHHHHHHEEHHCCCCHHHHHCCCCCCCCHHHHHCCCCCHH
SVLKHLRNISTKKIAIMLNEKNTTPEDLNHLLLPIIGLVDMIRIAIDPQNIDRAIVLAKA
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHH
IKTMGFEVGFNVMYMSKWAEMNGFLSKLKAIDKIADLFCMVDSFGGITPKEVKNLLKEVR
HHHCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KYTHVPVGFHGHDNLQLGLINSITAIDDGIDFIDATITGMGRGAGNLKMELLLTYLNKHH
HHHCCCCEECCCCCEEEEECHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
GLNVDFNVLGNIITTFTPLLEKYQWGTNLPYMLSGANNIPQKEVMDWVTNRVYSFNSIIR
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ALDNRKNKMEDNAKYPLLNIKNKFDRVVIIGGGFNAIEHKEAIKSFIETHTNTAIIFATA
HHHCCHHHCCCCCCCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEH
RYAREYLDVNAPHFYCLVGNEGHRLTHNINPQNLSGICVLPPYPRPMGTEVPEYAKNVTF
HHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCHHHHCCCEE
ELENITFIDQYKDSVTTIALQLAILLTDQDIYLVGYDGYPGNVLSEKEMALTNENRTIFA
EECCEEEEHHHHHHHHHHHHEEEEEEECCEEEEEEECCCCCCCCCCCHHEEECCCCEEEE
TYTTISGKILKSLTPSIYKEIEVVSVYQFI
EEEECCHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA