Click here to switch to the map view.

The map label for this gene is ilvG [C]

Identifier: 148827926

GI number: 148827926

Start: 1323226

End: 1323852

Strand: Direct

Name: ilvG [C]

Synonym: CGSHiGG_07155

Alternate gene names: 148827926

Gene position: 1323226-1323852 (Clockwise)

Preceding gene: 148827923

Following gene: 148827927

Centisome position: 70.12

GC content: 43.06

Gene sequence:

>627_bases
ATGACAGGTGCGCAACTTATCATGGCGTGCTTGAAAGCACATCATGTCACGACTCTTTTCGGCTATCCCGGTGGCGCAAT
TATGCCAACCTACGATGCCCTTTATGACGCAGGCTTAGATCACTTACTTTGTCGTAATGAACAAGGGACTGCAATGGCTG
CGATTGGCTATGCTCGTTCCACAGGAAAAGTTGGCGTCTGTATTGCAACATCAGGCCCTGGTGCCACTAATTTAGTTATT
GGATTAGGCGATGCCATGATGGACTCCATTCCAGTTGTCACTATTGAACCTTGGCGTGAACAAATCAGAGATTTAAAAGC
AAAATTAGATTTCACTTATGTTGAAAATCAAGGCAATCGCCCGATTGATCCTTGGGCATTACTCAACAGCCTATCTAATC
GCAAACCAAATAATGCGATAATTTGTACTGATGTGGGGCAGCATAAAATGTGGTCAGTGCAACACATTTTACGCGTAGCA
CGTCACTGCGGATTTACCGTTACAACAATGGAAATGACACTGATTGAAACCCAAGTGCGGTTAAAAATCACTGTAAAATC
TGACCGCACTTTTGATTTGTTAGTAAATCAGTTGGCGAAGTTACCGGATGTGATAACAATTAGATAG

Upstream 100 bases:

>100_bases
ACTTTTTTTCGTTTTTTTCTCTCCAAGCCCTACCAATGACTCGAAATTTCGCTACAATGCTGAACTTATCCCAAAATAAA
AACAACAGGACGCAAACACA

Downstream 100 bases:

>100_bases
GTAAACTATATGCCTGTTATCATTTTAGATCGAAATGTCATTAGTCTTATTAAAGATGTGAATTCAGGAAAAGTTATAAG
TGATAGCAATAAGCTAAATA

Product: UDP-2,3-diacylglucosamine hydrolase

Products: 2-acetolactate; CO2

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARSTGKVGVCIATSGPGATNLVI
GLGDAMMDSIPVVTIEPWREQIRDLKAKLDFTYVENQGNRPIDPWALLNSLSNRKPNNAIICTDVGQHKMWSVQHILRVA
RHCGFTVTTMEMTLIETQVRLKITVKSDRTFDLLVNQLAKLPDVITIR

Sequences:

>Translated_208_residues
MTGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARSTGKVGVCIATSGPGATNLVI
GLGDAMMDSIPVVTIEPWREQIRDLKAKLDFTYVENQGNRPIDPWALLNSLSNRKPNNAIICTDVGQHKMWSVQHILRVA
RHCGFTVTTMEMTLIETQVRLKITVKSDRTFDLLVNQLAKLPDVITIR
>Mature_207_residues
TGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARSTGKVGVCIATSGPGATNLVIG
LGDAMMDSIPVVTIEPWREQIRDLKAKLDFTYVENQGNRPIDPWALLNSLSNRKPNNAIICTDVGQHKMWSVQHILRVAR
HCGFTVTTMEMTLIETQVRLKITVKSDRTFDLLVNQLAKLPDVITIR

Specific function: Truncated acetolactase synthase; no longer catalytically active [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=96, Percent_Identity=48.9583333333333, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790104, Length=96, Percent_Identity=47.9166666666667, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1786717, Length=97, Percent_Identity=41.2371134020619, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=96, Percent_Identity=46.875, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012001 [H]

Pfam domain/function: PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6

Molecular weight: Translated: 22851; Mature: 22720

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARS
CCHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHCCCHHHEECCCCCCEEEEEEEECC
TGKVGVCIATSGPGATNLVIGLGDAMMDSIPVVTIEPWREQIRDLKAKLDFTYVENQGNR
CCCEEEEEEECCCCCCEEEEEECHHHHHCCCEEEEHHHHHHHHHHHHHCCEEEEECCCCC
PIDPWALLNSLSNRKPNNAIICTDVGQHKMWSVQHILRVARHCGFTVTTMEMTLIETQVR
CCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEE
LKITVKSDRTFDLLVNQLAKLPDVITIR
EEEEEECCCCHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
TGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARS
CHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHCCCHHHEECCCCCCEEEEEEEECC
TGKVGVCIATSGPGATNLVIGLGDAMMDSIPVVTIEPWREQIRDLKAKLDFTYVENQGNR
CCCEEEEEEECCCCCCEEEEEECHHHHHCCCEEEEHHHHHHHHHHHHHCCEEEEECCCCC
PIDPWALLNSLSNRKPNNAIICTDVGQHKMWSVQHILRVARHCGFTVTTMEMTLIETQVR
CCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEE
LKITVKSDRTFDLLVNQLAKLPDVITIR
EEEEEECCCCHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: pyruvate

Specific reaction: 2 pyruvate = 2-acetolactate + CO2

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]