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The map label for this gene is purB [H]

Identifier: 148827816

GI number: 148827816

Start: 1176705

End: 1178075

Strand: Direct

Name: purB [H]

Synonym: CGSHiGG_06455

Alternate gene names: 148827816

Gene position: 1176705-1178075 (Clockwise)

Preceding gene: 148827815

Following gene: 148827817

Centisome position: 62.35

GC content: 38.29

Gene sequence:

>1371_bases
ATGCAACTCTCTACACTGACCGCACTTTCCCCTCTTGATGGTCGTTATCAAGATAAAGTAACGCCATTACGTGCTATTTT
TAGCGAATTTGGGTTAATGAAATTCCGTGTAGCTGTGGAAGTTCGTTGGTTACAAAAATTAGCTTCAACGGCAGACATTA
CCGAAGTACCGCCATTTTCTACGCAAGCAAATGCTTTTTTAGATGGAATTGTGGCTAATTTTAATGAGGCCGATGCTGCG
CGTATTAAAGAAATTGAGCGAACAACCAATCACGATGTAAAGGCCGTTGAATATTTTCTGAAAGAGAAAATTCAAAATGA
AATTGAGCTAGTAAAAGTTTCTGAATTTATTCACTTCGCTTGCACCTCAGAAGATATTAACAACCTCTCTCACGCATTAA
TGTTAAGTACCGCTCGTGACGAAGTGATTTTACCAGAATGGCAAAAACTGATTGATGAAATCACTCGTCTTGCAGAAGAA
TATAAAACGATCCCATTACTTTCTCGCACACATGGTCAGCCAGCTTCACCAAGCACAGTGGGCAAAGAAATGGCAAATGT
CGTTTACCGCCTAAAACGTCAATTTAAACAACTCCAAAACGCGGAAATCTTAGGTAAAATCAATGGTGCAGTAGGTAACT
ACAATGCGCATTTATCGGCATATCCTAATATTGATTGGCATAAATTCAGCGAAGAATTTGTCACTTCATTAGGTATTCAA
TGGAATCCTTATACCACACAAATTGAGCCGCACGATTACATCGCTGAATTTTTTGATGCTGTAGTACGCTTTAATACCAT
TATCATTGATTTTGATCGTGATTTATGGGGATACATTGCATTAAATCACTTTAAACAACGAACTATTGCAGGCGAAATCG
GTTCTTCCACCATGCCACATAAAGTCAATCCAATTGATTTTGAAAACTCAGAGGGCAATCTAGGATTAGCTAATGCAGTG
ATGACCCACTTAGGTCAAAAGTTACCAATTTCTCGCTGGCAGCGTGACTTAACAGATTCAACCGTATTACGTAATTTAGG
CGTAGGATTAGGTTATTGTTTGATCGCTTATGCTTCAACTCGCAAAGGCATCAGTAAACTTGAAGTAAATCAACCGCACT
TATTAGAAGAACTCAATCAAAACTGGGAAGTTTTGGCTGAACCAATTCAAACCATAATGCGCCGCTATGGAATAGAAAAA
CCTTACGAAAAATTAAAAGAATTAACGCGAGGTAAACGAGTGACAGAACAGGCTATGCGTGAATTTATTGATAAGTTGGA
TATTCCACAAGAAGAAAAATTACGTTTACAAAAATTAACACCCGCTACTTATATTGGGGCTGCAGTGGAATTAGTAGAGA
AGCTATCATAG

Upstream 100 bases:

>100_bases
GGAACAAAATGGCAAATTTTATTTTTCCGCAGAAAATTACTTGCCACCGCAAAACAGATTTATTCTTCTATTTATTAATA
CAATTCTGGAGCAAACATTT

Downstream 100 bases:

>100_bases
GAATTTGGCTCTTTTTCCAACAATATTCTTTACAAGAACATAGCTTTTCTCTATAATCCTGCGCCTATGTTAGTCAATCT
AACAAATTTCTGGTTGGTGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFSTQANAFLDGIVANFNEADAA
RIKEIERTTNHDVKAVEYFLKEKIQNEIELVKVSEFIHFACTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEE
YKTIPLLSRTHGQPASPSTVGKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
WNPYTTQIEPHDYIAEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYASTRKGISKLEVNQPHLLEELNQNWEVLAEPIQTIMRRYGIEK
PYEKLKELTRGKRVTEQAMREFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS

Sequences:

>Translated_456_residues
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFSTQANAFLDGIVANFNEADAA
RIKEIERTTNHDVKAVEYFLKEKIQNEIELVKVSEFIHFACTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEE
YKTIPLLSRTHGQPASPSTVGKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
WNPYTTQIEPHDYIAEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYASTRKGISKLEVNQPHLLEELNQNWEVLAEPIQTIMRRYGIEK
PYEKLKELTRGKRVTEQAMREFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS
>Mature_456_residues
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFSTQANAFLDGIVANFNEADAA
RIKEIERTTNHDVKAVEYFLKEKIQNEIELVKVSEFIHFACTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEE
YKTIPLLSRTHGQPASPSTVGKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
WNPYTTQIEPHDYIAEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYASTRKGISKLEVNQPHLLEELNQNWEVLAEPIQTIMRRYGIEK
PYEKLKELTRGKRVTEQAMREFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=455, Percent_Identity=77.1428571428572, Blast_Score=755, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51992; Mature: 51992

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TQANAFLDGIVANFNEADAARIKEIERTTNHDVKAVEYFLKEKIQNEIELVKVSEFIHFA
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEEYKTIPLLSRTHGQPASPSTV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHH
GKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYAST
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RKGISKLEVNQPHLLEELNQNWEVLAEPIQTIMRRYGIEKPYEKLKELTRGKRVTEQAMR
HCCHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHH
EFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TQANAFLDGIVANFNEADAARIKEIERTTNHDVKAVEYFLKEKIQNEIELVKVSEFIHFA
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEEYKTIPLLSRTHGQPASPSTV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHH
GKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYAST
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RKGISKLEVNQPHLLEELNQNWEVLAEPIQTIMRRYGIEKPYEKLKELTRGKRVTEQAMR
HCCHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHH
EFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]