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The map label for this gene is dusA [H]

Identifier: 148827810

GI number: 148827810

Start: 1169598

End: 1170581

Strand: Direct

Name: dusA [H]

Synonym: CGSHiGG_06425

Alternate gene names: 148827810

Gene position: 1169598-1170581 (Clockwise)

Preceding gene: 148827809

Following gene: 148827812

Centisome position: 61.98

GC content: 40.24

Gene sequence:

>984_bases
ATGCTTCAAAATCAACCGCACTTTTATCGCGGCCGTTTTTCTGTTGCACCAATGTTAGATTGGACAACACGCCATTGTCG
TTATTTTCATCGCCAATTTTCAAAGCATGCGTTACTTTATACTGAAATGGTCACTGCACCTGCGATTATCCACGCAAAAT
ATGACCACTTGGATTTTGATTTACAAGAAAATCCCGTTGCGTTACAGCTGGGAGGAAGTGATCCTGAACAACTCAAACAT
TGTGCAAGACTTGCTGAGGAACGAGGCTATCACGAAATTAATCTTAATGTAGGATGCCCTTCTGATCGCGTACAAAATGG
AATGTTTGGTGCTTGCTTAATGGCGAAAGCAGATTTGGTGGCAGACTGTGTAGAACAAATGCAAAGTGCGGTCAAAATTC
CAGTCACTGTGAAAACGCGTATTGGCATTGATGAATTAGACAGCTATGAATTTTTATGTGATTTTATTGAAAAAGTTCAA
GGAAAAGGTTGCCAAGAGTTTATTATCCACGCACGAAAAGCTTGGCTTTCAGGGTTAAGTCCCAAAGAAAATAGAGAAAT
TCCGCCATTAGATTATACTCGGGTGTATCAACTAAAACGTGATTTTCCACATCTAACTATTGCAATTAATGGCGGTATAA
AAACCATTGAAGAAATGAAGCAACATTTGCAATATGTGGACGGCGTGATGGTTGGGCGAGAGGCTTATCAAAATCCAAGT
TTACTTGGGCAAATTGACCAAGCCTTATTTGATCCTAACGCACCAATTGTTACCGCTTATGAAGCGGTACAGTCCATGCT
TCCTTATATTGAACAACAACTTAGCCAAGGCATACACCTTAATCATATCGTCCGTCATATGTTAGGTGCATTCCAAAATT
GCAAAGGTGCTCGTCAATGGCGACGCTATTTAAGCGAAAATGCGTTTAAACAAGGTGCAGGAATTGAAGTGGTTGAAACA
GCCTTAAGCTTTGTAGAAAGCTAA

Upstream 100 bases:

>100_bases
CAAAACCATTTTACCACTTTGCTGCAGGCTGTTTTCGCCAGCAAATGCAAGCTCGCCAAGCCGAAGAATTACGCAGCAAA
ACCGAACAAGAGAAATAAAA

Downstream 100 bases:

>100_bases
TCCTAAAAACAAAATAAGCCGCAAAAGTGCGGCTTATTTTTCAGCTGTTTTTCTTACGCAATACGCATTTGAAAAATCAC
CAATTCAGCTTGACAACAAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFDLQENPVALQLGGSDPEQLKH
CARLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLVADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQ
GKGCQEFIIHARKAWLSGLSPKENREIPPLDYTRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPS
LLGQIDQALFDPNAPIVTAYEAVQSMLPYIEQQLSQGIHLNHIVRHMLGAFQNCKGARQWRRYLSENAFKQGAGIEVVET
ALSFVES

Sequences:

>Translated_327_residues
MLQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFDLQENPVALQLGGSDPEQLKH
CARLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLVADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQ
GKGCQEFIIHARKAWLSGLSPKENREIPPLDYTRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPS
LLGQIDQALFDPNAPIVTAYEAVQSMLPYIEQQLSQGIHLNHIVRHMLGAFQNCKGARQWRRYLSENAFKQGAGIEVVET
ALSFVES
>Mature_327_residues
MLQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFDLQENPVALQLGGSDPEQLKH
CARLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLVADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQ
GKGCQEFIIHARKAWLSGLSPKENREIPPLDYTRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPS
LLGQIDQALFDPNAPIVTAYEAVQSMLPYIEQQLSQGIHLNHIVRHMLGAFQNCKGARQWRRYLSENAFKQGAGIEVVET
ALSFVES

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=246, Percent_Identity=27.6422764227642, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI40807366, Length=252, Percent_Identity=26.5873015873016, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=70.440251572327, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1789660, Length=246, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788462, Length=245, Percent_Identity=26.9387755102041, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=27.3584905660377, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=242, Percent_Identity=26.4462809917355, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24580595, Length=260, Percent_Identity=24.6153846153846, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920448, Length=260, Percent_Identity=24.6153846153846, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37250; Mature: 37250

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFD
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
LQENPVALQLGGSDPEQLKHCARLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHH
ADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQGKGCQEFIIHARKAWLSGLS
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PKENREIPPLDYTRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPS
CCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLGQIDQALFDPNAPIVTAYEAVQSMLPYIEQQLSQGIHLNHIVRHMLGAFQNCKGARQW
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHH
RRYLSENAFKQGAGIEVVETALSFVES
HHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFD
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
LQENPVALQLGGSDPEQLKHCARLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHH
ADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQGKGCQEFIIHARKAWLSGLS
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PKENREIPPLDYTRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPS
CCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLGQIDQALFDPNAPIVTAYEAVQSMLPYIEQQLSQGIHLNHIVRHMLGAFQNCKGARQW
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHH
RRYLSENAFKQGAGIEVVETALSFVES
HHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]