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The map label for this gene is crr [H]

Identifier: 148825746

GI number: 148825746

Start: 706873

End: 707373

Strand: Direct

Name: crr [H]

Synonym: CGSHiEE_03475

Alternate gene names: 148825746

Gene position: 706873-707373 (Clockwise)

Preceding gene: 148825745

Following gene: 148825747

Centisome position: 38.99

GC content: 36.53

Gene sequence:

>501_bases
ATGGGCTTATTTGACAAGTTATTTGGTTCAAAAGAAAACAAATCTGTGGAAGTTGAAATTTATGCACCGATCTCTGGCGA
GATCGTTAATATTGAAGATGTTCCCGATGTGGTTTTCTCTGAAAAAATTGTTGGTGATGGTGTTGCTGTTCGTCCTATTG
GTAACAAAATCGTTGCACCAGTGGATGGTGTGATTGGTAAAATTTTTGAAACCAATCACGCTTTTTCAATGGAATCAAAA
GAAGGCGTAGAATTATTTGTACACTTTGGTATTGATACTGTTGAGTTAAAGGGGGAAGGCTTTACTCGTATTGCACAAGA
AGGGCAATCTGTTAAGCGTAGCGATACTGTGATTGAATTTGATCTTGCGCTATTAGAATCAAAAGCAAAATCTGTCTTAA
CACCAATCGTAATTTCAAATATGGACGAAATTTCTTGTATCGTAAAAAAATCAGGCGAAGTAGTGGCTGGAGAATCAGTG
GTATTAGCCTTAAAGAAATAA

Upstream 100 bases:

>100_bases
AATTAATTGAAGAATTTTTGCTGGAAAATTCATTAAATTAAACAAAATTATTTGATTTTACGTTAAAATAGCAGCCATCT
TGAATGGATAGGAGATTAAA

Downstream 100 bases:

>100_bases
TTTGTTTACGGTAAAAAAAGTCCGCCTTTGTGCGGACTTTTTATTTATTATAGTTACCTAGGGTAAGTATACCCTAGGCT
AATTTAAGTGCTACCCCGTT

Product: PTS system glucose-specific transporter subunit

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MGLFDKLFGSKENKSVEVEIYAPISGEIVNIEDVPDVVFSEKIVGDGVAVRPIGNKIVAPVDGVIGKIFETNHAFSMESK
EGVELFVHFGIDTVELKGEGFTRIAQEGQSVKRSDTVIEFDLALLESKAKSVLTPIVISNMDEISCIVKKSGEVVAGESV
VLALKK

Sequences:

>Translated_166_residues
MGLFDKLFGSKENKSVEVEIYAPISGEIVNIEDVPDVVFSEKIVGDGVAVRPIGNKIVAPVDGVIGKIFETNHAFSMESK
EGVELFVHFGIDTVELKGEGFTRIAQEGQSVKRSDTVIEFDLALLESKAKSVLTPIVISNMDEISCIVKKSGEVVAGESV
VLALKK
>Mature_165_residues
GLFDKLFGSKENKSVEVEIYAPISGEIVNIEDVPDVVFSEKIVGDGVAVRPIGNKIVAPVDGVIGKIFETNHAFSMESKE
GVELFVHFGIDTVELKGEGFTRIAQEGQSVKRSDTVIEFDLALLESKAKSVLTPIVISNMDEISCIVKKSGEVVAGESVV
LALKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=169, Percent_Identity=72.7810650887574, Blast_Score=235, Evalue=9e-64,
Organism=Escherichia coli, GI1786894, Length=149, Percent_Identity=45.6375838926174, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1790159, Length=161, Percent_Identity=33.5403726708075, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17882; Mature: 17751

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLFDKLFGSKENKSVEVEIYAPISGEIVNIEDVPDVVFSEKIVGDGVAVRPIGNKIVAP
CCCHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHCCCCCEEEEECCCEEECC
VDGVIGKIFETNHAFSMESKEGVELFVHFGIDTVELKGEGFTRIAQEGQSVKRSDTVIEF
HHHHHHHHHCCCCEECCCCCCCEEEEEEECCEEEEECCCCHHHHHHCCCCCCCCCCEEEE
DLALLESKAKSVLTPIVISNMDEISCIVKKSGEVVAGESVVLALKK
HHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCEEECCEEEEEEEC
>Mature Secondary Structure 
GLFDKLFGSKENKSVEVEIYAPISGEIVNIEDVPDVVFSEKIVGDGVAVRPIGNKIVAP
CCHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHCCCCCEEEEECCCEEECC
VDGVIGKIFETNHAFSMESKEGVELFVHFGIDTVELKGEGFTRIAQEGQSVKRSDTVIEF
HHHHHHHHHCCCCEECCCCCCCEEEEEEECCEEEEECCCCHHHHHHCCCCCCCCCCEEEE
DLALLESKAKSVLTPIVISNMDEISCIVKKSGEVVAGESVVLALKK
HHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]