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The map label for this gene is ptsH [H]

Identifier: 148825744

GI number: 148825744

Start: 704749

End: 705006

Strand: Direct

Name: ptsH [H]

Synonym: CGSHiEE_03465

Alternate gene names: 148825744

Gene position: 704749-705006 (Clockwise)

Preceding gene: 148825743

Following gene: 148825745

Centisome position: 38.87

GC content: 39.53

Gene sequence:

>258_bases
ATGTATTCAAAAGATGTGGAAATTATTGCACCAAACGGTTTACACACGCGCCCTGCTGCACAATTTGTGAAAGAAGCGAA
AGCATTTTCTTCTGAAATTACAGTAACCTCAGGGGGGAAAAGTGCAAGTGCAAAGAGCTTATTTAAGTTGCAAACATTAG
TTTTAACGCAGGGAACAATACTTACCATTTCTGCTGATGGAGAAGACGAGCAACAGGCTGTAGACCATTTAGTCGCGTTA
ATTCCAACTTTAGAATAA

Upstream 100 bases:

>100_bases
GCTTTTTAGTGTTTATTCTTGATTATTTTGTTTTGAAAAAGGATACTAGGGCAATTTTCTGGTTTTATATTTTTAATTAT
TGATTAGTCGAGGTAGCATT

Downstream 100 bases:

>100_bases
TTCTTACTTAGCCATAAAATTGTGAAATTTTATGGCTATTGTTTATTTATTACTTTGTTAATTGTTTCGGAAGGTATCTA
TGATTTCTGGCATTCTTGCA

Product: ribosome-associated GTPase

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein [H]

Number of amino acids: Translated: 85; Mature: 85

Protein sequence:

>85_residues
MYSKDVEIIAPNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLVLTQGTILTISADGEDEQQAVDHLVAL
IPTLE

Sequences:

>Translated_85_residues
MYSKDVEIIAPNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLVLTQGTILTISADGEDEQQAVDHLVAL
IPTLE
>Mature_85_residues
MYSKDVEIIAPNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLVLTQGTILTISADGEDEQQAVDHLVAL
IPTLE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788755, Length=85, Percent_Identity=77.6470588235294, Blast_Score=136, Evalue=2e-34,

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 9028; Mature: 9028

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00369 PTS_HPR_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSKDVEIIAPNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLVLTQGTI
CCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHEECCCEE
LTISADGEDEQQAVDHLVALIPTLE
EEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYSKDVEIIAPNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLVLTQGTI
CCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHEECCCEE
LTISADGEDEQQAVDHLVALIPTLE
EEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]