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The map label for this gene is mcl2 [H]

Identifier: 148824267

GI number: 148824267

Start: 3449814

End: 3450737

Strand: Reverse

Name: mcl2 [H]

Synonym: TBFG_13092

Alternate gene names: 148824267

Gene position: 3450737-3449814 (Counterclockwise)

Preceding gene: 148824271

Following gene: 148824265

Centisome position: 77.99

GC content: 64.07

Gene sequence:

>924_bases
ATGACCTCAATGTATGAACAGGTCGATACCAACACGGCGGATCCGGTGGCCGGGTCTCGTATCGATCCGGTTCTCGCGCG
GAGTTGGTTACTGGTCAACGGCGCGCACGGCGACCGGTTCGAGTCCGCCGCGCACTCACGCGCCGACATCGTCGTGCTCG
ATATCGAGGACGCGGTCGCTCCCAAAGACAAGCATGCCGCCCGGGACAACGCCGTGCGTTGGTTTGGTGACGGAAACGCT
GACTGGGTGCGCATCAACGGCTTCGGCACACCTTGGTGGGCGGACGATCTAGCGATGTTGGCCGATAGCCCCGTTGGCGG
AGTGATGCTGGCGATGGTCGAATCGGTGGACCATGTCACCGAGACCGCGAAACGACTACCTAACGTGCCGATCGTTGCAC
TGGTCGAAACGGCGCGGGGTCTGGAGCGCATCAACGAGATTGCTGCGGCCAAGGGCACCTTCCGGCTCGCCTTCGGTATC
GGCGACTTTCGCCGGGACACCGGCTTTGGCGAAGACCCGGCAACATTGGCCTACGCGCGGTCGCGCTTTACCATCGCGGC
CCGGGCCGCCGGTCTGCCCAGCGCGATCGACGGGCCGACCATCGGCTCCAACGCGCTCAAGCTGATCGAGGCCACGGCCG
TCTCCGCCGAGTTTGGCATGACCGGCAAGATCTGCCTTTCGCCGGACCAATGTCCGGTGGTGAATGAGGGGCTATCCCCG
TCGCAGGACGAAATCGTCTGGGCGAAGGAATTTTTCGCTGAGTTCGCCCGCGACGGGGGCGAGATCCGCAACGGGTCTGA
CCTGCCGCGCATTGCCCGGGCCACCAAGATCCTCGATCTGGCCCGCGCCTACGGCATCGAAGTGTCCGACTTCGAGGACG
AACCGGTTCACATGCCGGCTCCGACTGACACCTACCACTACTAG

Upstream 100 bases:

>100_bases
ATGGACGTGGGTTTTGCGGGGCATCCTGCCAATCCGATTTGCGACGTCGCTACGGTTTAAGGAGAACAATGTCGACATCG
ACTGAGCGAAGAGACACTAG

Downstream 100 bases:

>100_bases
GCGGCGTATCGAGCGAGCGCGATGCCGATTCGGACCTCGAGTTCGCTGACGGTGACCGCAGGCAGGTGCGGCGGGGCGCC
GGACCGGTGTCGACTGCCTG

Product: hypothetical protein

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTSMYEQVDTNTADPVAGSRIDPVLARSWLLVNGAHGDRFESAAHSRADIVVLDIEDAVAPKDKHAARDNAVRWFGDGNA
DWVRINGFGTPWWADDLAMLADSPVGGVMLAMVESVDHVTETAKRLPNVPIVALVETARGLERINEIAAAKGTFRLAFGI
GDFRRDTGFGEDPATLAYARSRFTIAARAAGLPSAIDGPTIGSNALKLIEATAVSAEFGMTGKICLSPDQCPVVNEGLSP
SQDEIVWAKEFFAEFARDGGEIRNGSDLPRIARATKILDLARAYGIEVSDFEDEPVHMPAPTDTYHY

Sequences:

>Translated_307_residues
MTSMYEQVDTNTADPVAGSRIDPVLARSWLLVNGAHGDRFESAAHSRADIVVLDIEDAVAPKDKHAARDNAVRWFGDGNA
DWVRINGFGTPWWADDLAMLADSPVGGVMLAMVESVDHVTETAKRLPNVPIVALVETARGLERINEIAAAKGTFRLAFGI
GDFRRDTGFGEDPATLAYARSRFTIAARAAGLPSAIDGPTIGSNALKLIEATAVSAEFGMTGKICLSPDQCPVVNEGLSP
SQDEIVWAKEFFAEFARDGGEIRNGSDLPRIARATKILDLARAYGIEVSDFEDEPVHMPAPTDTYHY
>Mature_306_residues
TSMYEQVDTNTADPVAGSRIDPVLARSWLLVNGAHGDRFESAAHSRADIVVLDIEDAVAPKDKHAARDNAVRWFGDGNAD
WVRINGFGTPWWADDLAMLADSPVGGVMLAMVESVDHVTETAKRLPNVPIVALVETARGLERINEIAAAKGTFRLAFGIG
DFRRDTGFGEDPATLAYARSRFTIAARAAGLPSAIDGPTIGSNALKLIEATAVSAEFGMTGKICLSPDQCPVVNEGLSPS
QDEIVWAKEFFAEFARDGGEIRNGSDLPRIARATKILDLARAYGIEVSDFEDEPVHMPAPTDTYHY

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=290, Percent_Identity=30, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI87081764, Length=238, Percent_Identity=24.3697478991597, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 33100; Mature: 32969

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSMYEQVDTNTADPVAGSRIDPVLARSWLLVNGAHGDRFESAAHSRADIVVLDIEDAVA
CCCHHHHHCCCCCCCCCCCCCCHHHHCCEEEEECCCCCHHHHHHCCCCCEEEEECCCCCC
PKDKHAARDNAVRWFGDGNADWVRINGFGTPWWADDLAMLADSPVGGVMLAMVESVDHVT
CCHHHHHCCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
ETAKRLPNVPIVALVETARGLERINEIAAAKGTFRLAFGIGDFRRDTGFGEDPATLAYAR
HHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCHHHHHHH
SRFTIAARAAGLPSAIDGPTIGSNALKLIEATAVSAEFGMTGKICLSPDQCPVVNEGLSP
HHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCC
SQDEIVWAKEFFAEFARDGGEIRNGSDLPRIARATKILDLARAYGIEVSDFEDEPVHMPA
CHHHEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCEECCC
PTDTYHY
CCCCCCC
>Mature Secondary Structure 
TSMYEQVDTNTADPVAGSRIDPVLARSWLLVNGAHGDRFESAAHSRADIVVLDIEDAVA
CCHHHHHCCCCCCCCCCCCCCHHHHCCEEEEECCCCCHHHHHHCCCCCEEEEECCCCCC
PKDKHAARDNAVRWFGDGNADWVRINGFGTPWWADDLAMLADSPVGGVMLAMVESVDHVT
CCHHHHHCCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
ETAKRLPNVPIVALVETARGLERINEIAAAKGTFRLAFGIGDFRRDTGFGEDPATLAYAR
HHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCHHHHHHH
SRFTIAARAAGLPSAIDGPTIGSNALKLIEATAVSAEFGMTGKICLSPDQCPVVNEGLSP
HHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCC
SQDEIVWAKEFFAEFARDGGEIRNGSDLPRIARATKILDLARAYGIEVSDFEDEPVHMPA
CHHHEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCEECCC
PTDTYHY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA