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The map label for this gene is iscS [H]

Identifier: 148824216

GI number: 148824216

Start: 3395595

End: 3396776

Strand: Reverse

Name: iscS [H]

Synonym: TBFG_13041

Alternate gene names: 148824216

Gene position: 3396776-3395595 (Counterclockwise)

Preceding gene: 148824217

Following gene: 148824215

Centisome position: 76.77

GC content: 66.07

Gene sequence:

>1182_bases
ATGGCCTACCTGGATCACGCTGCCACCACCCCGATGCACCCCGCCGCCATCGAGGCGATGGCGGCCGTGCAGCGCACCAT
CGGCAATGCGTCGTCGCTGCACACCAGCGGGCGCTCGGCGCGCCGGCGGATCGAGGAGGCCCGTGAGCTGATCGCGGACA
AGCTAGGCGCTCGTCCGTCCGAGGTGATCTTCACCGCGGGCGGCACCGAAAGCGACAACCTGGCTGTCAAAGGTATCTAT
TGGGCACGCCGCGATGCGGAGCCGCACCGCCGTCGCATCGTCACCACCGAGGTGGAACACCACGCCGTACTGGACTCGGT
GAACTGGCTCGTGGAACACGAAGGCGCCCATGTGACCTGGCTGCCGACCGCCGCCGACGGCTCGGTGTCGGCAACTGCGC
TGCGCGAGGCACTGCAGAGCCACGACGACGTCGCGCTGGTATCGGTGATGTGGGCCAACAACGAGGTCGGAACTATTCTA
CCGATCGCCGAAATGTCAGTTGTCGCCATGGAATTCGGCGTGCCGATGCACAGTGATGCCATTCAGGCGGTGGGACAGCT
CCCGCTTGACTTCGGGGCCAGCGGGCTGTCGGCGATGAGCGTGGCCGGGCACAAATTCGGTGGCCCGCCAGGAGTGGGTG
CGTTGCTGCTGCGCCGCGACGTCACCTGCGTGCCCCTTATGCACGGCGGTGGGCAGGAGCGCGATATTCGTTCCGGCACA
CCCGATGTCGCCAGTGCAGTTGGAATGGCGACGGCCGCGCAGATCGCGGTGGACGGACTCGAGGAAAACAGCGCGCGGTT
ACGGCTGCTGCGGGATCGTCTGGTCGAGGGTGTGCTGGCTGAGATTGACGATGTTTGCCTTAACGGCGCCGATGACCCGA
TGCGGCTAGCGGGTAACGCGCACTTCACTTTCCGTGGCTGCGAAGGCGATGCGCTGTTGATGTTGTTGGACGCTAACGGA
ATCGAGTGCTCAACCGGATCGGCCTGCACGGCAGGTGTAGCGCAGCCCTCGCATGTGTTGATTGCAATGGGCGTCGACGC
GGCCAGCGCCCGCGGATCATTGCGTCTCTCGCTGGGGCACACCAGTGTTGAGGCTGATGTCGATGCCGCGTTGGAGGTGC
TTCCCGGGGCGGTGGCACGTGCACGGCGGGCCGCCCTAGCCGCCGCGGGAGCATCCCGATGA

Upstream 100 bases:

>100_bases
CTAAGTGGTCTGGCCCCAGCCAGATTGCCCAGCCGCTGCTGCGGCCGTGGCCTGCGCGCACGTTCTGCGTCGCCGCCCGG
GCCAGTATTCTAGGTGCGTC

Downstream 100 bases:

>100_bases
AAGTTCTCGCCGCGATGAGCGGTGGCGTCGACTCGTCTGTTGCTGCCGCCCGCATGGTCGATGCCGGACACGAGGTGGTC
GGAGTGCACATGGCGCTATC

Product: cysteine desulfurase iscS

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAYLDHAATTPMHPAAIEAMAAVQRTIGNASSLHTSGRSARRRIEEARELIADKLGARPSEVIFTAGGTESDNLAVKGIY
WARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTWLPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTIL
PIAEMSVVAMEFGVPMHSDAIQAVGQLPLDFGASGLSAMSVAGHKFGGPPGVGALLLRRDVTCVPLMHGGGQERDIRSGT
PDVASAVGMATAAQIAVDGLEENSARLRLLRDRLVEGVLAEIDDVCLNGADDPMRLAGNAHFTFRGCEGDALLMLLDANG
IECSTGSACTAGVAQPSHVLIAMGVDAASARGSLRLSLGHTSVEADVDAALEVLPGAVARARRAALAAAGASR

Sequences:

>Translated_393_residues
MAYLDHAATTPMHPAAIEAMAAVQRTIGNASSLHTSGRSARRRIEEARELIADKLGARPSEVIFTAGGTESDNLAVKGIY
WARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTWLPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTIL
PIAEMSVVAMEFGVPMHSDAIQAVGQLPLDFGASGLSAMSVAGHKFGGPPGVGALLLRRDVTCVPLMHGGGQERDIRSGT
PDVASAVGMATAAQIAVDGLEENSARLRLLRDRLVEGVLAEIDDVCLNGADDPMRLAGNAHFTFRGCEGDALLMLLDANG
IECSTGSACTAGVAQPSHVLIAMGVDAASARGSLRLSLGHTSVEADVDAALEVLPGAVARARRAALAAAGASR
>Mature_392_residues
AYLDHAATTPMHPAAIEAMAAVQRTIGNASSLHTSGRSARRRIEEARELIADKLGARPSEVIFTAGGTESDNLAVKGIYW
ARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTWLPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTILP
IAEMSVVAMEFGVPMHSDAIQAVGQLPLDFGASGLSAMSVAGHKFGGPPGVGALLLRRDVTCVPLMHGGGQERDIRSGTP
DVASAVGMATAAQIAVDGLEENSARLRLLRDRLVEGVLAEIDDVCLNGADDPMRLAGNAHFTFRGCEGDALLMLLDANGI
ECSTGSACTAGVAQPSHVLIAMGVDAASARGSLRLSLGHTSVEADVDAALEVLPGAVARARRAALAAAGASR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=384, Percent_Identity=39.3229166666667, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=33.3333333333333, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=40.5684754521964, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1787970, Length=388, Percent_Identity=26.0309278350515, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789175, Length=403, Percent_Identity=28.287841191067, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=36.4583333333333, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=30.5295950155763, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=36.1979166666667, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI20129463, Length=384, Percent_Identity=39.0625, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40948; Mature: 40817

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYLDHAATTPMHPAAIEAMAAVQRTIGNASSLHTSGRSARRRIEEARELIADKLGARPS
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
EVIFTAGGTESDNLAVKGIYWARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTW
CEEEECCCCCCCCEEEEEEEEECCCCCHHHHEEEEEEHHHHHHHHHHHHHEEECCCEEEE
LPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTILPIAEMSVVAMEFGVPMHSDA
EECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEHHHHHEEHHHHCCCCCHHH
IQAVGQLPLDFGASGLSAMSVAGHKFGGPPGVGALLLRRDVTCVPLMHGGGQERDIRSGT
HHHHHCCCHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCC
PDVASAVGMATAAQIAVDGLEENSARLRLLRDRLVEGVLAEIDDVCLNGADDPMRLAGNA
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
HFTFRGCEGDALLMLLDANGIECSTGSACTAGVAQPSHVLIAMGVDAASARGSLRLSLGH
EEEEECCCCCEEEEEEECCCCEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECC
TSVEADVDAALEVLPGAVARARRAALAAAGASR
CCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AYLDHAATTPMHPAAIEAMAAVQRTIGNASSLHTSGRSARRRIEEARELIADKLGARPS
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
EVIFTAGGTESDNLAVKGIYWARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTW
CEEEECCCCCCCCEEEEEEEEECCCCCHHHHEEEEEEHHHHHHHHHHHHHEEECCCEEEE
LPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTILPIAEMSVVAMEFGVPMHSDA
EECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEHHHHHEEHHHHCCCCCHHH
IQAVGQLPLDFGASGLSAMSVAGHKFGGPPGVGALLLRRDVTCVPLMHGGGQERDIRSGT
HHHHHCCCHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCC
PDVASAVGMATAAQIAVDGLEENSARLRLLRDRLVEGVLAEIDDVCLNGADDPMRLAGNA
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
HFTFRGCEGDALLMLLDANGIECSTGSACTAGVAQPSHVLIAMGVDAASARGSLRLSLGH
EEEEECCCCCEEEEEEECCCCEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECC
TSVEADVDAALEVLPGAVARARRAALAAAGASR
CCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]