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The map label for this gene is ddl

Identifier: 148824171

GI number: 148824171

Start: 3348505

End: 3349626

Strand: Reverse

Name: ddl

Synonym: TBFG_12996

Alternate gene names: 148824171

Gene position: 3349626-3348505 (Counterclockwise)

Preceding gene: 148824172

Following gene: 148824169

Centisome position: 75.71

GC content: 67.56

Gene sequence:

>1122_bases
GTGAGTGCTAACGACCGGCGTGATCGGCGTGTCCGCGTTGCCGTCGTGTTCGGCGGGCGCAGCAACGAGCACGCCATCTC
GTGTGTGTCCGCCGGCAGCATCCTGCGCAACCTGGACTCGCGGCGGTTCGACGTGATCGCGGTGGGTATCACCCCGGCAG
GTTCGTGGGTGCTCACCGACGCCAACCCCGACGCCCTGACGATCACCAACCGGGAGCTTCCTCAGGTCAAATCAGGATCG
GGCACCGAGCTGGCGCTGCCGGCCGATCCGCGGCGTGGTGGCCAGTTGGTGTCGCTGCCGCCCGGGGCCGGCGAGGTTCT
GGAGTCGGTCGACGTGGTGTTCCCGGTACTGCACGGCCCGTACGGCGAGGACGGCACGATCCAGGGACTGCTCGAACTCG
CCGGGGTGCCCTACGTGGGCGCCGGTGTGCTGGCCAGTGCCGTCGGCATGGACAAGGAGTTCACCAAGAAGCTGCTCGCC
GCCGATGGACTTCCGGTGGGTGCGTACGCGGTGCTGCGTCCGCCGCGGTCGACACTGCACCGCCAGGAGTGCGAACGGCT
GGGCTTACCGGTGTTCGTCAAACCCGCCCGAGGCGGCTCGTCGATCGGTGTTAGCCGGGTGTCGAGTTGGGATCAACTGC
CCGCCGCGGTCGCGCGGGCCCGCCGGCATGACCCTAAGGTCATCGTCGAGGCCGCGATCAGCGGCCGCGAGCTGGAATGC
GGTGTGCTCGAAATGCCGGACGGCACACTGGAAGCCAGCACGCTGGGGGAGATCCGGGTGGCCGGGGTGCGGGGACGCGA
GGACTCTTTCTACGACTTCGCAACCAAGTATCTCGACGACGCAGCCGAATTGGACGTGCCCGCCAAGGTCGATGACCAGG
TCGCAGAGGCGATTCGTCAGCTGGCGATCCGGGCGTTCGCGGCTATCGACTGCCGGGGTCTGGCCAGGGTGGACTTCTTC
CTCACCGACGACGGTCCGGTGATCAACGAGATCAACACGATGCCGGGATTCACCACGATCTCGATGTACCCGCGGATGTG
GGCGGCCAGCGGTGTCGACTATCCGACCCTGCTGGCGACGATGATCGAGACGGCATTGGCCCGCGGCGTGGGCCTGCACT
AG

Upstream 100 bases:

>100_bases
GTCGCCGCACCAAGCCCGAATGAGCCTCGCACGGGCCGGCACGACCGAAAACTGCAGCTCGTGGGAGCCCGTCTGCCTGA
CCCGGTAGCCTCTGCAGGTT

Downstream 100 bases:

>100_bases
CCCGATGGTGCCGACCCGCCGCGCCCGGCGCTAGCGCCGCGCTTGCGATCGGCACTTAGCCCGATGGTGCCGACCCGCCG
CGCCCGGCGCTAGCGCCGCG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTDANPDALTITNRELPQVKSGS
GTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLA
ADGLPVGAYAVLRPPRSTLHRQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELEC
GVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQLAIRAFAAIDCRGLARVDFF
LTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLATMIETALARGVGLH

Sequences:

>Translated_373_residues
MSANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTDANPDALTITNRELPQVKSGS
GTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLA
ADGLPVGAYAVLRPPRSTLHRQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELEC
GVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQLAIRAFAAIDCRGLARVDFF
LTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLATMIETALARGVGLH
>Mature_372_residues
SANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTDANPDALTITNRELPQVKSGSG
TELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLAA
DGLPVGAYAVLRPPRSTLHRQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELECG
VLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQLAIRAFAAIDCRGLARVDFFL
TDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLATMIETALARGVGLH

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=364, Percent_Identity=43.6813186813187, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1786280, Length=260, Percent_Identity=36.5384615384615, Blast_Score=158, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_MYCBP (A1KMX5)

Other databases:

- EMBL:   AM408590
- RefSeq:   YP_979086.1
- ProteinModelPortal:   A1KMX5
- SMR:   A1KMX5
- STRING:   A1KMX5
- EnsemblBacteria:   EBMYCT00000021162
- GeneID:   4698165
- GenomeReviews:   AM408590_GR
- KEGG:   mbb:BCG_3002c
- eggNOG:   COG1181
- GeneTree:   EBGT00050000015718
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   MBOV410289:BCG_3002C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39681; Mature: 39549

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTD
CCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEEECCCCCEEEEC
ANPDALTITNRELPQVKSGSGTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGP
CCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
YGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLAADGLPVGAYAVLRPPRSTLH
CCCCCHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHH
RQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELEC
HHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCEECC
GVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQ
CEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LAIRAFAAIDCRGLARVDFFLTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLAT
HHHHHHHHHCCCCCEEEEEEECCCCCEEHHCCCCCCEEEEECCHHHHHCCCCCHHHHHHH
MIETALARGVGLH
HHHHHHHHCCCCC
>Mature Secondary Structure 
SANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTD
CCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEEECCCCCEEEEC
ANPDALTITNRELPQVKSGSGTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGP
CCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
YGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLAADGLPVGAYAVLRPPRSTLH
CCCCCHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHH
RQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELEC
HHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCEECC
GVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQ
CEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LAIRAFAAIDCRGLARVDFFLTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLAT
HHHHHHHHHCCCCCEEEEEEECCCCCEEHHCCCCCCEEEEECCHHHHHCCCCCHHHHHHH
MIETALARGVGLH
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA