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The map label for this gene is mtr

Identifier: 148824045

GI number: 148824045

Start: 3176843

End: 3178222

Strand: Direct

Name: mtr

Synonym: TBFG_12870

Alternate gene names: 148824045

Gene position: 3176843-3178222 (Clockwise)

Preceding gene: 148824044

Following gene: 148824046

Centisome position: 71.8

GC content: 62.68

Gene sequence:

>1380_bases
ATGGAAACGTACGACATCGCGATCATCGGAACCGGTTCGGGCAACAGCATTCTCGACGAACGCTATGCCAGCAAGCGGGC
GGCGATCTGCGAGCAGGGCACCTTCGGCGGCACCTGCCTCAATGTCGGGTGCATCCCCACAAAAATGTTCGTCTACGCCG
CCGAGGTGGCCAAGACCATCCGAGGCGCGTCGCGTTACGGTATCGACGCGCACATCGACCGGGTGCGATGGGACGACGTC
GTCTCGCGCGTCTTCGGGCGCATCGATCCGATCGCGCTGAGCGGCGAGGACTATCGAAGGTGTGCGCCCAACATCGACGT
GTACCGCACACACACCCGTTTCGGGCCGGTTCAGGCCGATGGCCGCTACCTGTTGCGCACTGACGCGGGTGAAGAGTTCA
CCGCCGAGCAGGTGGTGATAGCCGCCGGATCGCGGCCGGTGATTCCGCCGGCCATCCTCGCGTCCGGCGTCGACTATCAC
ACCAGCGATACCGTCATGCGGATCGCCGAGTTGCCGGAGCACATCGTGATCGTCGGAAGCGGCTTCATTGCAGCGGAATT
CGCACATGTGTTTTCCGCTCTGGGCGTACGGGTCACCCTGGTGATCCGGGGCAGCTGCTTACTACGGCATTGTGACGACA
CCATCTGCGAACGGTTCACCCGCATCGCATCGACCAAATGGGAGCTGCGCACCCATCGCAACGTTGTGGACGGCCAGCAG
CGCGGCTCGGGCGTCGCGCTGCGGCTAGACGATGGTTGCACCATCAACGCCGACCTACTGTTGGTAGCGACAGGCCGGGT
GTCCAACGCCGACCTGCTGGATGCCGAGCAGGCCGGTGTCGATGTCGAGGACGGCCGGGTGATAGTCGACGAGTACCAAC
GGACTTCGGCGCGTGGGGTTTTTGCGCTGGGCGATGTCTCGTCGCCGTACTTGCTCAAGCATGTCGCCAACCACGAGGCC
CGCGTCGTGCAGCACAATCTGCTCTGCGACTGGGAGGACACCCAGTCGATGATCGTCACCGACCACCGATACGTACCGGC
TGCGGTATTCACCGATCCTCAGATCGCTGCCGTCGGACTCACTGAAAACCAAGCTGTGGCAAAGGGACTCGATATTTCGG
TCAAGATACAGGACTATGGTGACGTCGCGTACGGCTGGGCGATGGAGGACACCAGTGGAATCGTCAAGCTCATCACCGAG
CGCGGCTCTGGGCGCTTACTGGGCGCACACATCATGGGTTACCAGGCATCCTCGCTCATCCAACCGTTGATCCAGGCGAT
GAGCTTTGGGCTGACCGCCGCCGAAATGGCCCGCGGCCAGTACTGGATTCATCCGGCGCTGCCGGAGGTGGTGGAAAACG
CGCTGCTTGGCCTGCGTTGA

Upstream 100 bases:

>100_bases
GGCTTATCGCCGACTGGATCTCTGGTTGGACGACTACCTCGGCACACACAACGACACCGACGCTTCGGCATCGTCGGGGA
AAGGGTGATGGCCCCTACAA

Downstream 100 bases:

>100_bases
CCGCAACGGCGAGCCGTCGTCCGGCAAGCGATTTGCATCCCGTCAGCGCCTTACCTACAGTCGGGACATCGCGTTCTGCC
CCGTGCTGGAAGGACCGACA

Product: mycothione reductase

Products: NA

Alternate protein names: Mycothiol-disulfide reductase; NADPH-dependent mycothione reductase

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
METYDIAIIGTGSGNSILDERYASKRAAICEQGTFGGTCLNVGCIPTKMFVYAAEVAKTIRGASRYGIDAHIDRVRWDDV
VSRVFGRIDPIALSGEDYRRCAPNIDVYRTHTRFGPVQADGRYLLRTDAGEEFTAEQVVIAAGSRPVIPPAILASGVDYH
TSDTVMRIAELPEHIVIVGSGFIAAEFAHVFSALGVRVTLVIRGSCLLRHCDDTICERFTRIASTKWELRTHRNVVDGQQ
RGSGVALRLDDGCTINADLLLVATGRVSNADLLDAEQAGVDVEDGRVIVDEYQRTSARGVFALGDVSSPYLLKHVANHEA
RVVQHNLLCDWEDTQSMIVTDHRYVPAAVFTDPQIAAVGLTENQAVAKGLDISVKIQDYGDVAYGWAMEDTSGIVKLITE
RGSGRLLGAHIMGYQASSLIQPLIQAMSFGLTAAEMARGQYWIHPALPEVVENALLGLR

Sequences:

>Translated_459_residues
METYDIAIIGTGSGNSILDERYASKRAAICEQGTFGGTCLNVGCIPTKMFVYAAEVAKTIRGASRYGIDAHIDRVRWDDV
VSRVFGRIDPIALSGEDYRRCAPNIDVYRTHTRFGPVQADGRYLLRTDAGEEFTAEQVVIAAGSRPVIPPAILASGVDYH
TSDTVMRIAELPEHIVIVGSGFIAAEFAHVFSALGVRVTLVIRGSCLLRHCDDTICERFTRIASTKWELRTHRNVVDGQQ
RGSGVALRLDDGCTINADLLLVATGRVSNADLLDAEQAGVDVEDGRVIVDEYQRTSARGVFALGDVSSPYLLKHVANHEA
RVVQHNLLCDWEDTQSMIVTDHRYVPAAVFTDPQIAAVGLTENQAVAKGLDISVKIQDYGDVAYGWAMEDTSGIVKLITE
RGSGRLLGAHIMGYQASSLIQPLIQAMSFGLTAAEMARGQYWIHPALPEVVENALLGLR
>Mature_459_residues
METYDIAIIGTGSGNSILDERYASKRAAICEQGTFGGTCLNVGCIPTKMFVYAAEVAKTIRGASRYGIDAHIDRVRWDDV
VSRVFGRIDPIALSGEDYRRCAPNIDVYRTHTRFGPVQADGRYLLRTDAGEEFTAEQVVIAAGSRPVIPPAILASGVDYH
TSDTVMRIAELPEHIVIVGSGFIAAEFAHVFSALGVRVTLVIRGSCLLRHCDDTICERFTRIASTKWELRTHRNVVDGQQ
RGSGVALRLDDGCTINADLLLVATGRVSNADLLDAEQAGVDVEDGRVIVDEYQRTSARGVFALGDVSSPYLLKHVANHEA
RVVQHNLLCDWEDTQSMIVTDHRYVPAAVFTDPQIAAVGLTENQAVAKGLDISVKIQDYGDVAYGWAMEDTSGIVKLITE
RGSGRLLGAHIMGYQASSLIQPLIQAMSFGLTAAEMARGQYWIHPALPEVVENALLGLR

Specific function: Catalyzes the NAD(P)H-dependent reduction of mycothione (the oxidized disulfide form of mycothiol) to mycothiol

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI50301238, Length=441, Percent_Identity=29.0249433106576, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=26.6524520255864, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI22035672, Length=449, Percent_Identity=27.8396436525612, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI291045266, Length=448, Percent_Identity=25.4464285714286, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33519430, Length=441, Percent_Identity=24.7165532879819, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI33519428, Length=441, Percent_Identity=24.7165532879819, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI33519426, Length=441, Percent_Identity=24.7165532879819, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI148277065, Length=441, Percent_Identity=24.7165532879819, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI148277071, Length=441, Percent_Identity=24.7165532879819, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI291045268, Length=448, Percent_Identity=23.2142857142857, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=30.2540415704388, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786307, Length=431, Percent_Identity=28.538283062645, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=28.3582089552239, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87082354, Length=431, Percent_Identity=25.0580046403712, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32565766, Length=474, Percent_Identity=27.8481012658228, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=27.6018099547511, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=27.6018099547511, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=28.2105263157895, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325166, Length=449, Percent_Identity=26.0579064587973, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=26.1410788381743, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=22.5469728601253, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21358499, Length=480, Percent_Identity=25.2083333333333, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17737741, Length=482, Percent_Identity=25.7261410788382, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 650 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): MTR_MYCTU (O07927)

Other databases:

- EMBL:   AF002193
- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   B70590
- RefSeq:   NP_337433.1
- RefSeq:   YP_177910.1
- HSSP:   P06715
- ProteinModelPortal:   O07927
- SMR:   O07927
- EnsemblBacteria:   EBMYCT00000000665
- EnsemblBacteria:   EBMYCT00000071006
- GeneID:   887773
- GeneID:   925355
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2922
- KEGG:   mtu:Rv2855
- TIGR:   MT2922
- TubercuList:   Rv2855
- GeneTree:   EBGT00050000014575
- HOGENOM:   HBG515043
- OMA:   DTIMRIS
- ProtClustDB:   PRK07846
- BioCyc:   MetaCyc:MONOMER-9685
- GO:   GO:0005737
- GO:   GO:0040007
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017817
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PRINTS:   PR00368
- TIGRFAMs:   TIGR03452

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.15

Molecular weight: Translated: 49946; Mature: 49946

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 444-444

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METYDIAIIGTGSGNSILDERYASKRAAICEQGTFGGTCLNVGCIPTKMFVYAAEVAKTI
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
RGASRYGIDAHIDRVRWDDVVSRVFGRIDPIALSGEDYRRCAPNIDVYRTHTRFGPVQAD
HCCHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCEEEEECCCCCEECC
GRYLLRTDAGEEFTAEQVVIAAGSRPVIPPAILASGVDYHTSDTVMRIAELPEHIVIVGS
CCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHCCCEEEEEEC
GFIAAEFAHVFSALGVRVTLVIRGSCLLRHCDDTICERFTRIASTKWELRTHRNVVDGQQ
CHHHHHHHHHHHHCCEEEEEEEECCHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCC
RGSGVALRLDDGCTINADLLLVATGRVSNADLLDAEQAGVDVEDGRVIVDEYQRTSARGV
CCCEEEEEECCCCEECCCEEEEEECCCCCCCCCCHHHCCCCCCCCEEEEEHHHCCCCCCE
FALGDVSSPYLLKHVANHEARVVQHNLLCDWEDTQSMIVTDHRYVPAAVFTDPQIAAVGL
EEECCCCCHHHHHHHCCCCCEEEHHHEEECCCCCCCEEEECCCCCCEEEECCCCEEEEEC
TENQAVAKGLDISVKIQDYGDVAYGWAMEDTSGIVKLITERGSGRLLGAHIMGYQASSLI
CCCCHHHCCCEEEEEEECCCCEEEEEEECCCCCHHHHEECCCCCCEEEEEEECCCHHHHH
QPLIQAMSFGLTAAEMARGQYWIHPALPEVVENALLGLR
HHHHHHHHCCCCHHHHHCCCEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
METYDIAIIGTGSGNSILDERYASKRAAICEQGTFGGTCLNVGCIPTKMFVYAAEVAKTI
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
RGASRYGIDAHIDRVRWDDVVSRVFGRIDPIALSGEDYRRCAPNIDVYRTHTRFGPVQAD
HCCHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCEEEEECCCCCEECC
GRYLLRTDAGEEFTAEQVVIAAGSRPVIPPAILASGVDYHTSDTVMRIAELPEHIVIVGS
CCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHCCCEEEEEEC
GFIAAEFAHVFSALGVRVTLVIRGSCLLRHCDDTICERFTRIASTKWELRTHRNVVDGQQ
CHHHHHHHHHHHHCCEEEEEEEECCHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCC
RGSGVALRLDDGCTINADLLLVATGRVSNADLLDAEQAGVDVEDGRVIVDEYQRTSARGV
CCCEEEEEECCCCEECCCEEEEEECCCCCCCCCCHHHCCCCCCCCEEEEEHHHCCCCCCE
FALGDVSSPYLLKHVANHEARVVQHNLLCDWEDTQSMIVTDHRYVPAAVFTDPQIAAVGL
EEECCCCCHHHHHHHCCCCCEEEHHHEEECCCCCCCEEEECCCCCCEEEECCCCEEEEEC
TENQAVAKGLDISVKIQDYGDVAYGWAMEDTSGIVKLITERGSGRLLGAHIMGYQASSLI
CCCCHHHCCCEEEEEEECCCCEEEEEEECCCCCHHHHEECCCCCCEEEEEEECCCHHHHH
QPLIQAMSFGLTAAEMARGQYWIHPALPEVVENALLGLR
HHHHHHHHCCCCHHHHHCCCEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036